Outlet fittings are provided. Outlet fittings of interest include an elongate structure and an opening at a proximal end for receiving a flow stream from the distal end of a flow cell. In addition, the outlet fittings described herein are configured to reduce the formation of bubbles at the interface between the outlet fitting and the flow cell. In certain cases, outlet fittings do not include a planar surface in contact with the received flow stream. Flow cytometers and methods employing the subject outlet fittings are also provided.

The present disclosure provides circulation system and method for detecting immune adherence of porcine erythrocytes. The system includes: a flow chamber, a gasket, a mobile-phase liquid storage bottle, a peristaltic pump, silicone hoses, and a cell culture dish provided with a glass slide, where the gasket is placed on an edge of the glass slide, and the flow chamber is covered on the gasket; the flow chamber, the mobile-phase liquid storage bottle, and the peristaltic pump are connected in sequence with the silicone hoses to form the circulation system; and a porcine erythrocyte suspension of immune adherence-sensitized GFP-E. coli is injected into the mobile-phase liquid storage bottle. In the present disclosure, the circulation system and method for detecting immune adherence of porcine erythrocytes can be used for detection and analysis of working parameters such as a mobile phase flow velocity, a shear force, and a maximum retention time.

A flow cytometer can include: at least one light emitter configured to emit light in a light path; a rectangular flow cell having flow cell width that is substantially lateral to the light path and a flow cell depth that is longitudinal to the light path, wherein the light path has an interrogation width at the flow cell that is narrower than the flow cell width; and a spherical reflector positioned adjacent to the rectangular flow cell and having a concave reflective surface that has a reflective direction that is positioned substantially orthogonal with the light path such that reflected light is reflected along a reflected path that is substantially orthogonal with the light path. At least one light absorbing member is positioned at least partially around the reflected path to absorb reflected light at an angle to the reflected path.

Apparatus and method for separating whole cells from a mixture, e.g., including liquid, other cell types, nucleic acid material, or other components. Focused acoustic energy may be used to move whole cells in a chamber so that the cells exit the chamber via a first outlet rather than a second outlet. A filter may, or need not, be used to assist in separation.

The present invention relates generally to the field of plastic microparticles. In particular, the present invention relates to the detection of plastic microparticles in a water-based sample. An embodiment of the present invention relates to a process for detecting and characterizing plastic microparticles in a water-based sample comprising the analysis of the sample by spectral flow cytometry. In accordance with the present invention, the process described herein may comprise the processing of the recorded flow cytometry data by a machine learning algorithm that can distinguish and categorize each particle based on its unique spectrum to characterize, for example, the plastic microparticles.

A flow cell of the flow cytometer of the present invention includes: a sample flow path through which a sample fluid containing a sample flows; and a sample fluid supply portion which communicates with an upstream end of the sample flow path in the sample fluid flow direction and supplies the sample fluid to the sample flow path, wherein the sample fluid supply portion includes a plurality of sample opening portions which supply a sample fluid to the sample flow path, a cleaning liquid supply opening portion to which a second tube is connectable and which supplies a cleaning liquid for cleaning the sample fluid supply portion, and a cleaning liquid discharge opening portion to which a first tube is connectable and which discharges the cleaning liquid from the sample fluid supply portion.

Provided herein are techniques for label free cell sorting. The systems and methods provided herein may use machine learning based image classification techniques to identify cells of interest within a sample of cells. The cells of interest may then be separated from the sample using mechanical, pneumatic, piezoelectric, and/or electronic devices.

Light detection systems are provided. Aspects of the light detection systems include first and second light receivers in fixed positions relative to each other, a plurality of wavelength separators configured to pass light from the first and second light receivers having a predetermined spectral range, and a plurality of light detection modules. Baseplates having a stage for mounting a light receiver, a plurality of recesses for fixing a plurality of light detection modules in rigid alignment relative to the stage, and a heat dissipation opening positioned within each recess are also provided. In addition, particle analysis systems, methods and kits for practicing the invention are disclosed.

A data receiver thread is continuously executed to receive in which signals indicating a fluid parameter. A predetermined time quantity of the signals is repeatedly buffered. Upon completion of the buffering of each predetermined time quantity of the signals, a data processing thread is initiated that executes on the just completed buffered predetermined time quantity of signals. Upon completion of each data processing thread, data from the just completed data processing thread is passed to a data plotting thread. Results of the data plotting thread are displayed on a portable electronic device while the data receiver thread is being executed.

Fluid may be pumped within a microfluidic channel across a cell/particle sensor using a microscopic resistor. The microscopic resistor may be selectively actuated so as to heat the fluid within the microfluidic channel to a temperature below a nucleation energy of the fluid so as to regulate a temperature of the fluid for at least when the cell/particle sensor is sensing the fluid.