Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.

Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

Aspects of the present disclosure include methods and systems for automated analysis of clinical fluid samples, such as urine, blood, or cerebral spinal fluid, where the number of fluid samples in increased or optimized without negatively impacting the accuracy of the analysis of a given fluid sample.

An imaging flow cytometer includes: a laser unit that emits first and second laser light to first and second spots; a first and second imaging sections that image the first and second spots; first and second detection devices that detect a particle that passes through the first and second spots; a first particle detection section that issues an imaging timing instruction signal to the first and second imaging sections; an image storage section that receives an image imaged by the first and second imaging sections; and a sorting determination section that determines whether the particle is an objective particle. The first and second imaging sections clip an image of the particle based on the imaging timing instruction signal.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

Provided herein are techniques for label free cell sorting. The systems and methods provided herein may use machine learning based image classification techniques to identify cells of interest within a sample of cells. The cells of interest may then be separated from the sample using mechanical, pneumatic, piezoelectric, and/or electronic devices.

Aspects of the present disclosure include systems for irradiating particles in a flow stream. Systems according to certain embodiments include a light source having a first laser configured for continuous irradiation of a flow stream and a second laser configured for irradiation of the flow stream in discrete intervals where each discrete interval of irradiation by the second laser is triggered by irradiation of a particle in the flow stream with the first laser. Methods for irradiating a sample in a flow stream with the subject light sources are also described. Computer readable storage medium for practicing the subject methods are provided. Kits having one or more lasers are also provided.

A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.

The present disclosure relates to quality control for point-of-care medical diagnostic systems. In various embodiments, the system includes an on-board storage containing a synthetic quality control material, a plurality of sub-systems having a plurality of operating parameters and including a material analyzer, a database storing quality control results that include results of the material analyzer analyzing the synthetic quality control material over time, one or more processors, and at least one memory storing instructions which, when executed by the one or more processors, cause the system to, automatically without user intervention: generate a control chart based on the quality control results, determine that a parameter of the plurality of operating parameters is out-of-tolerance based on the control chart, and adjust at least one of the plurality of sub-systems without user intervention to bring the out-of-tolerance parameter to within tolerance.

In certain embodiments a device is provided for electrorotation flow. In certain embodiments the device comprises a microfluidic channel comprising a plurality of electrodes disposed to provide dielectrophoretic (DEP) forces that are perpendicular to hydrodynamic flows along the channel; and a fluid within the channel providing the hydrodynamic flow along the channel; wherein the device is configured to apply focusing voltages to the electrodes that provide an electric field minimum in the channel and that focus cells, particles, and/or molecules or molecular complexes within the channel; and where the device is configured to apply rotation-inducing voltages to the electrodes that induce rotation of the cells, particles, molecules and/or molecular complexes as they flow through the channel.