Systems and methods for mounting of material samples via a vacuum system and controlling fluid flow through a tube of the vacuum system are disclosed. In some examples, the vacuum system may be a castable and/or cold mounting vacuum system that facilitates mounting and/or encapsulation of material samples in epoxy resin under low, vacuum, and/or near vacuum pressure. In some examples, the vacuum system may comprise a flow control device configured to control epoxy flow through a dispensing tube that connects to a hollow vacuum chamber. In some examples, the vacuum chamber may have an opening defined by a rim sandwiched between upper and lower portions of a sealing ring. A movable lid may be configured to press down on the upper portion of the sealing ring when in a closed position, so as to seal the opening.

A holding portion includes a first holding portion and a second holding portion that respectively hold a first cassette holder and a second cassette holder, a chemical liquid bottle holding portion including a first chemical liquid bottle holding portion and a second chemical liquid bottle holding portion, and a paraffin-filled bottle holding portion including a first paraffin-filled bottle holding portion and a second paraffin-filled bottle holding portion. The first cassette holder is inserted into the chemical liquid bottle provided to the first chemical liquid bottle holding portion and the paraffin-filled bottle provided to the first paraffin-filled bottle holding portion. Furthermore, the second cassette holder is inserted into the chemical liquid bottle provided to the second chemical liquid bottle holding portion and the paraffin-filled bottle provided to the second paraffin-filled bottle holding portion.

A spheroid tissue microarray comprises an array of tissue spheroids embedded within a porous mold. The product may be impregnated with a wax or resin and sectioned, and contains spheroids which are precisely located in a regular geometric grid. A method of manufacturing a spheroid tissue microarray comprises the steps of: forming a mold of porous material from liquid mold material in a casting mold, and allowing the liquid mold material to set; removing the porous mold from the casting mold; topping up the porous mold with further liquid mold material, and allowing recesses to form in the surface of the mold by the drawing-in of liquid mold material through shrinkage as the liquid mold material sets; placing tissue spheroids into the recesses in the surface of the porous mold; and sealing the tissue spheroids within the mold by topping off with liquid mold material and allowing the liquid mold material to set. An alternative method comprises the steps of: forming a mold of porous material from liquid mold material in a casting mold; allowing the liquid mold material to set; removing the porous mold from the casting mold; placing spheroids in recesses at the bases of wells in the mold of porous material; and sealing the spheroids within the porous mold by adding further porous material on top of the spheroids; wherein the recesses at the bases of the wells in the porous material are formed by protrusions of the casting mold carrying further, nipple-shaped, protrusions.

Provided is a method for fixing particles on a two-dimensional filter medium with open pores. The particles are to be fixed on the upper side of the filter medium. The method involves contacting the particles with an adhesive solution from the lower side of the filter medium through the pores. The method makes it possible to fix particles in a simple and inexpensive manner to the filter medium, such that subsequent microscopic or SEM/EDX investigation is impaired as little as possible.

A TEM electromechanical in-situ testing method of one-dimensional materials is provided. A multi-function sample stage which can compress, buckle and bend samples is designed and manufactured. A carbon film on a TEM grid of Cu is eliminated, and the TEM grid of Cu is cut in half through the center of the circle. The samples are dispersed ultrasonically in alcohol and dropped on the edge of the semicircular grid of Cu with a pipette. A single sample is fixed on the edge of a substrate of the sample stage with conductive silver epoxy by using a micromechanical device under an optical microscope, and conductive silver paint is applied to the surface of the substrate of the sample stage; and an electromechanical in-situ testing is conducted in a TEM. This provides a simple and efficient sample preparation and testing method for a TEM electromechanical in-situ observing experiment.

A composite core material and methods for making same are disclosed herein. The composite core material comprises mineral filler discontinuous portions disposed in a continuous encapsulating resin. Further, the method for forming a composite core material comprises the steps of forming a mixture comprising mineral filler, an encapsulating prepolymer, and a polymerization catalyst; disposing the mixture onto a moving belt; and polymerizing said encapsulating prepolymer to form a composite core material comprising mineral filler discontinuous portions disposed in a continuous encapsulating resin.

Systems and methods for mounting of material samples via a vacuum system and controlling fluid flow through a tube of the vacuum system are disclosed. In some examples, the vacuum system may be a castable and/or cold mounting vacuum system that facilitates mounting and/or encapsulation of material samples in epoxy resin under low, vacuum, and/or near vacuum pressure. In some examples, the vacuum system may comprise a flow control device configured to control epoxy flow through a dispensing tube that connects to a hollow vacuum chamber. In some examples, the vacuum chamber may have an opening defined by a rim sandwiched between upper and lower portions of a sealing ring. A movable lid may be configured to press down on the upper portion of the sealing ring when in a closed position, so as to seal the opening.

Vacuum systems for epoxy mounting of material samples are disclosed. In some examples, a vacuum system may be a castable and/or cold mounting vacuum system that facilitates mounting and/or encapsulation of material samples in epoxy resin under low, vacuum, and/or near vacuum pressure. In some examples, the vacuum system may comprise a flow control device configured to control epoxy flow through a dispensing tube that connects to a hollow vacuum chamber. In some examples, the vacuum chamber may have an opening encircled by a rim sandwiched between upper and lower portions of a sealing ring. A movable lid may be configured to press down on the upper portion of the sealing ring when in a closed position, so as to seal the opening.

The invention provides a method for preparing an expanded biological specimen suitable for microscopic analysis. Expanding the biological sample can be achieved by anchoring biomolecules to a polymer network and swelling, or expanding, the polymer network, thereby moving the biomolecules apart as further described below. As the biomolecules are anchored to the polymer network isotropic expansion of the polymer network retains the spatial orientation of the biomolecules resulting in an expanded, or enlarged, biological specimen.

A method for visualizing neurons is provided. The method comprises a staining step and a visualizing step. The staining step comprises following steps: placing a neural tissue sample in an acrolein solution in the dark for fixation; placing the fixed neural tissue sample in a Golgi-Cox solution in the dark; replacing the Golgi-Cox solution; incubating the neural tissue sample placed in the replaced Golgi-Cox solution at a range of 36° C. to 38° C.; gradiently dehydrating the neural tissue sample; and embedding the dehydrated neural tissue sample with Petropoxy 154 resin. The visualizing step comprises: performing data acquisition and image reconstruction on the neural tissue sample using X-ray microscopy.