Provided herein are methods of preserving a biological sample on a first substrate, the methods including: (a) disposing a biological sample on a first substrate; (b) delivering a spacer agent to the first substrate, wherein the spacer agent is applied around the biological sample on the first substrate, thereby generating a chamber around the biological sample; (c) delivering a mounting agent to the chamber; and (d) assembling the first substrate with a second substrate, thereby preserving the biological sample on the first substrate.

The present invention relates to a porous material for inclusion of cytological preparations such as for example the material taken from procedures of fine needle aspiration with high effectiveness level. The effectiveness consists in quantitative and qualitative advantages: the proposed porous material has a high affinity for the cellular material which is captured and kept in the meshes by forming a kind of tissue without losing cellular elements thus with a quantitative advantage with respect to the traditional methods. Moreover, the material proposed in the patent is provided with wide cells delimited by thin meshes, this allows a wide diffusion of the fixative by optimizing the morphology preservation of the cytological sample; such qualitative advantage translates into optimum yield of the ancillary methods for studying the pathology.

A staining method includes staining a biological sample with a coumarin fluorescent dye to provide a fluorescent-stained sample, and bringing the fluorescent-stained sample into contact with osmium tetroxide, further embedding the sample in an epoxy resin, and subsequently slicing the sample to provide a section sample including the fluorescent-stained sample.

A manufacturing method of an embedded sample block includes providing a carrier. The carrier has a sample accommodating area and a marking area. The sample accommodating area has a first groove and the marking area has second grooves. A sample is disposed in the first groove and a molding plate standing around the carrier is formed. The molding plate surrounds the sample accommodating area and the marking area and forms an opening exposing the sample, the first groove and the second grove. A molding material is formed inside the opening, such that the molding material covers the sample and is filled into the first and second grooves. The molding material is solidified and the molding plate is removed to obtain the embedded sample block. In addition, a sample sheet sliced from the embedded sample block is also mentioned.

A specimen for analyzing the shape of an antistatic antifouling layer and a method for preparing the same are provided. The specimen includes Pt coated on an antistatic antifouling layer comprising a conductive polymer formed on a polymer substrate, so that a contrast difference between the polymer substrate layer and the antistatic antifouling layer is caused by the diffusion of Pt by means of the conductive polymer and the dyeing effect of the antifouling layer. Accordingly, it is possible to clearly distinguish the shape of the antistatic antifouling layer formed on the polymer substrate by using TEM.

This disclosure provides a biological sample processing system and methods for processing biological samples. The processing may include one or both of removing wax from wax-embedded biological samples and applying a reagent to biological samples. Aspects of the dewaxing technique and the technique for applying the reagent may be combined to achieve energy efficiencies. Additional efficiency may be achieved by recirculating heated reagent. A hybrid power system may use a source of stored power to supplement external power at times of peak demand. Uniform distribution of liquids across a surface of the biological samples may be achieved by self-leveling using an inclinometer.

The disclosure provides improved materials and methods for optically clearing biological tissue that is subsequently used for deep tissue imaging analysis. Also provided is a description of a microscopic image acquisition methodology in which imagery of intact tissues are acquired to rapidly acquire microscopy data on a whole-organ scale to maximize cost effectiveness for biological microscopy and minimize time spent performing such analysis.

Methods and kits useful in expansion microscopy are described. In particular, the present disclosure relates to methods and kits for expanding or enlarging fixed samples of interest for microscopy by synthesizing a water-swellable compound within a fixed sample, which can be physically expanded, resulting in physical magnification of the sample. Furthermore, the methods and kits disclosed allow the use of fluorescent proteins expressed within the sample and/or the use of standard fluorophore-labeled secondary antibodies (referred to as conventional secondary antibodies) in expansion microscopy (ExM). Thus, conventional secondary antibodies and/or fluorescent proteins expressed within the sample can be used with conventional immunostaining for the optical imaging of a sample of interest with resolution better than the standard microscopy diffraction limit.

This disclosure is directed to solutions and methods of using the solutions. A first solution includes a polymer, a polyol, and a buffer. A second solution includes a polymer, a polyol, and an alcohol.

Milling with ultraviolet excitation (MUVE) realizes high-throughput multiplex imaging of large three-dimensional samples. The instrumentation may comprise a UV-source attachment, precision stage attachment, and/or a blade assembly, and the instrumentation may overcome several constraints inherent to current state-of-the-art three-dimensional microscopy. MUVE offers throughput that is orders of magnitude faster than other technology by collecting a two-dimensional array of pixels simultaneously. The proposed instrumentation also utilizes serial ablation and provides the opportunity for true whole-organ imaging at microscopic resolution.