Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided.

An in-vitro platelet evaluation method, including creating a platelet suspension sample and flowing the platelet suspension sample through an evaluation system to determine a platelet aggregation area and a platelet rolling velocity.

Embodiments of a platelet testing system include an analyzer console device and a blood testing cartridge configured to releasably install into the console device. The cartridge device is configured with one or more measuring chambers and one or more mixing chambers that are fluidically connected within the cartridge device that enable the mixing of saline and a blood sample to a desired dilution. Additionally, the cartridge device is further configured with a cartridge slider that provides a reagent bead to the saline and blood mixture at a desired time. As such, one or more platelet activation assays can be conducted by measuring, through cartridge electrodes of the cartridge device, the detectable changes in platelet activity within the blood and saline mixture.

Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided.

The invention relates to a device for examining particles in a liquid to be examined, comprising a flow passage through which the liquid to be examined is moved. The flow passage has at least one inlet through which at least one sheath fluid flows into the flow passage such that the at least one sheath fluid forms at least one sheath flow in the flow passage. The device further comprises a wave generating device for piezoacoustically generating sound waves which propagate through the flow passage transversely to the flow direction of the liquid to be examined and form wave nodes on a monitoring plane such that particles to be examined of the liquid to be examined are moved onto the monitoring plane and accumulate thereon on the basis of the pressure effect of the sound waves in the transverse direction.

Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.

Devices and methods for measuring analytes and target particles in a sample are disclosed. In some embodiments, the disclosure provides a cartridge device. In other embodiments, the disclosure provides a method of using a cartridge device as disclosed herein for analyzing analytes and target particles in a sample. In further embodiments, the disclosure provides an analyzer including a cartridge device and a control unit device. The control unit device is configured to receive, operate, and/or actuate the cartridge device. In some embodiments, the disclosure provides a method of using an analyzer as disclosed herein for analyzing analytes and target particles in a sample.

The present disclosure includes provides methods for analyzing biological samples to identify, classify, and/or quantify platelets in the sample. The present disclosure also provides systems and methods for analyzing a blood sample to determine presence of platelet clumps in the sample. Also provided are systems configured for performing the disclosed methods and computer readable medium storing instructions for performing steps of the disclosed methods.

A sample analyzer with an optical detection device and a sample analysis method of the sample analyzer are disclosed. The optical detection device includes a fluid chamber, a light source and a light detector. The fluid chamber includes an illumination zone. An analyte flows through the illumination zone so as to form a sample stream. The light source illuminates the illumination zone to excite cell articles, reacted with a reagent, of the sample stream to emit a light signal. The light detector detects the fluorescent lights and transforms it into an electric signal. The light detector can include a silicon photomultiplier.

In some instances, an apparatus can include a light sensitive imaging sensor having a surface to receive a fluid sample, a body to be moved relative to the light sensitive imaging sensor and having a surface to touch a portion of the fluid sample, and a carrier to move the body toward the surface of the light sensitive imaging sensor to cause the surface of the body to touch the portion of the fluid sample, so that as the surface of the body touches the portion of the fluid, the surface of the body (i) is parallel to the surface of the light sensitive imaging sensor, and (ii) settles on top of the fluid sample independently of motion of the carrier.