A centrifugal field-flow fractionation device is provided with a controller for controlling introduction of a liquid sample by a sample introduction part and rotation of the rotor by a motor. The controller rotates the rotor at a first rotational speed when introducing the liquid sample into a flow path. The first rotational speed is faster than a second rotational speed. Therefore, when a liquid sample is introduced into the flow path, high centrifugal force can be applied to the liquid sample, which can suppress the variation of the particles in the liquid sample introduced to the flow path. The controller rotates the rotor at the second rotational speed slower than the first rotational speed with the liquid sample flow in the flow path stopped. Therefore, it is possible to prevent the constant application of high centrifugal force to the liquid sample, thereby suppressing the increase in the length of time required for analysis.

A microfluidic diagnostic chip may comprise a main fluid channel comprising a main pump, a secondary fluid channel branching off from the main fluid channel, and a secondary pump within the secondary fluid channel wherein the secondary pump is to pull a particle of analyte of a first size from a fluid passing through the main channel, the fluid comprising particles of analyte of the first size and of a number of larger sizes. A method of analyzing an analyte on a microfluidic chip may comprise pumping, with a main microfluidic pump, a fluid comprising an analyte particle through a main microfluidic channel fluidly coupled to a fluid slot and sorting the analyte particle within the fluid through a secondary microfluidic channel by pulling the analyte particle into the secondary microfluidic channel with a secondary microfluidic pump.

Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

An apparatus and a method for isolating and/or preparing particles are provided.

A flow path device includes a first portion, and a second portion. The first portion includes a resin first body and a first reinforcement. In the first body, a first connector connects a first outer portion and a first joint having a groove pattern defining a first flow path.

The first reinforcement is between and bonded to the first outer portion and the first joint, and includes first protrusions protruding from the first body and including two specific-shaped portions. The second portion includes a resin second body and a second reinforcement. In the second body, a second connector connects a second outer portion and a second joint, and through-holes connect to the first flow path. The second reinforcement is between and bonded to the second outer portion and the second joint, and includes second protrusions protruding from the second body and including two specific-shaped portions.

In example implementations, an apparatus is provided. The apparatus includes a channel, a camera, and a processor. The channel contains a fluid and an object. The fluid is to move the object through the channel. The camera system is to capture video images of the object in the channel. The processor is to track movement of the object in the channel via the video images based on known flow dynamics of the channel.

A device (100) for visualization of one or more components in a blood sample is disclosed. In one aspect, the device (100) includes an imaging module (110), wherein the imaging module (110) includes a controllable illumination source (102) capable of emitting light in plurality of discrete angles; a tube lens (105); one or more objective lens (104); and an image capturing module (106). Additionally, the device (100) includes a channel (103) configured to carry the blood sample, wherein the channel (103) is capable of sorting the one or more components in the blood sample.

The objective of the present invention is to provide a particle detection device and a particle detection method that can individually and continuously detect a wide range of particles. The objective is achieved by a particle detection device including: a particle separation channel through which particles are separated according to particle sizes in a perpendicular direction to the flow of fluid; and two or more particle recovery channels that are connected to and branched from the particle separation channel, in which each of the particle recovery channels includes a particle detection unit that includes an aperture and an electric detector.

The present invention provides a magnetic sorting microfluidic chip, including a substrate, a chip model material layer, a micro-channel unit and a magnetic sorting unit, where the chip model material layer is disposed on the substrate, and the micro-channel unit and the magnetic sorting unit are both disposed in the chip model material layer; the micro-channel unit includes a sorting channel and magnetic pole channels; the sorting channel is provided with a plurality of sorting channel inlets and a plurality of sorting channel outlets; and the magnetic sorting unit includes permanent magnets, high-permeability alloys, and magnetic pole arrays disposed in the magnetic pole channels, where the high-permeability alloys are configured to conduct magnetic fields of the permanent magnets to the magnetic pole arrays, so that the magnetic pole arrays generate magnetic fields having opposite polarities on left and right positions of the sorting channel.

A flow-type field-flow fractionation apparatus 1 includes a first heater 14 and a second heater 16. The first heater 14 heats a carrier fluid between a first pump 12 and a separation cell 3. The second heater 16 heats a focus fluid between a second pump 15 and the separation cell 3. Thus, the carrier fluid heated by the first heater 14 is sent by the first pump 12 and flows into the separation cell 3, and the focus fluid heated by the second heater 16 is sent by the second pump 15 and flows into the separation cell 3. This can stabilize temperatures of the carrier fluid and the focus fluid flowing into the separation cell 3. Then, when an analysis is performed using the flow-type field-flow fractionation apparatus 1, the analysis can be performed with high reproducibility.