A system and method for the label-free analysis of cells includes a purification device configured to receive a heterogeneous population of cells, the purification device temporarily trapping therein a subpopulation of cells from the heterogeneous population of cells and a cell analysis device positioned downstream of the purification device and configured to measure one or more cellular parameters including cell count, measured cell size, and/or cell morphology. In an alternative embodiment, the subpopulation of cells is analyzed while they are trapped within the purification device.

[Solving Means] A flow channel device includes a vessel layer and a flow channel layer. The vessel layer includes an injection vessel section into which a fluid including a sample is injected and a collection vessel section that collects the fluid. The flow channel layer includes a flow channel connected to the injection vessel section and the collection vessel section and is bonded to the vessel layer.

Disclosed are a microfluidic system (1) configured to receive cell populations and further configured to simultaneously isolate and quantify at least one sub-population of cells for each cell population, and related methods of using the system. The system comprises a substrate having networks of microchannels comprising a first sorting unit configured to isolate, by magnetic attraction, cells of interest in the population in at least one first sorting microchannel. The network comprises a second unit for simultaneous sorting and counting comprising at least one second sorting microchannel defined by a closed wall having an inner face provided with at least one functionalised electrode configured to trap a sub-population. The second unit further comprising means for counting the sub-population by impedance spectroscopy. The second sorting microchannel can have at least one pair of opposing functionalised electrodes and at least one pair of second micro-coils for trapping the cells of interest arranged in the wall facing the electrodes and controlling successive attraction/release cycles that alternate between the micro-coils.

The device (100) comprises a cavity (101) and at least two microporous membranes (102), wherein the microporous membranes (102) are arranged in series in the cavity (101) and divide the cavity (101) into a plurality of chambers (1011); each of the microporous membranes (102) is provided with micropores (103), and two adjacent chambers (1011) are in communication via the micropores (103); and each of the chambers (1011) is provided with an electrode (1012).