Apparatus and method for separating whole cells from a mixture, e.g., including liquid, other cell types, nucleic acid material, or other components. Focused acoustic energy may be used to move whole cells in a chamber so that the cells exit the chamber via a first outlet rather than a second outlet. A filter may, or need not, be used to assist in separation.

An airborne, gas, or liquid particle sensor with multiple particle sensor blocks in a single particle counter. Each sensor would sample a portion of the incoming airstream, or possibly a separate airstream. The various counters could be used separately or in concert.

The disclosed apparatus, systems and methods relate to technology that provides a method for the assessment of the polymerization of a sample, e.g., whole blood or blood plasma coagulation, by a non-contact acoustic tweezing device via the application of a sweeping frequency to the levitating sample and the corresponding assessment of extracted sample parameters.

Provided herein are techniques for label free cell sorting. The systems and methods provided herein may use machine learning based image classification techniques to identify cells of interest within a sample of cells. The cells of interest may then be separated from the sample using mechanical, pneumatic, piezoelectric, and/or electronic devices.

The Nondestructive Fluid Sensing System is a device that rapidly scans fluids to determine physical and chemical properties of the sample fluid. The Nondestructive Fluid Sensing System can detect the presence of a sample fluid with various optical and electrical sensors, and determines physical and chemical properties. The system features several innovations that increase sample throughput, reduces sample cross contamination, and eliminates waste products typically used in chemical tests. The system may be applied to various industries including manufacturing quality control, and healthcare.

Apparatus and methods are described use with a bodily sample that contains cells. A microscope (24) is focused, such that a focal plane of the microscope (24) at least approximately coincides with a level at which at least some cells belonging to the sample are at least partially disposed. At least one on-focus microscopic image of the sample, while the focal plane of the microscope (24) approximately coincides with the level. The microscope (24) is focused such that the focal plane of the microscope is offset with respect to the level, at least one off-focus microscopic image of the sample is acquired, while the focal plane of the microscope (24) is offset with respect to the level. A property of at least a portion of the sample is determined, at least partially based upon the on-focus and off-focus images. Other applications are also described.

A data receiver thread is continuously executed to receive in which signals indicating a fluid parameter. A predetermined time quantity of the signals is repeatedly buffered. Upon completion of the buffering of each predetermined time quantity of the signals, a data processing thread is initiated that executes on the just completed buffered predetermined time quantity of signals. Upon completion of each data processing thread, data from the just completed data processing thread is passed to a data plotting thread. Results of the data plotting thread are displayed on a portable electronic device while the data receiver thread is being executed.

An in-line holographic microscope can be used to analyze on a frame-by-frame basis a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. Through a combination of applying a combination of Lorenz-Mie analysis with selected hardware and software methods, this analysis can be carried out in near real time. An efficient particle identification methodology automates initial position estimation with sufficient accuracy to enable unattended holographic tracking and characterization.

A method for evaluating a biological material for unassociated virus-like particles virus size having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-like particle and fluorescent antibody stain.

Fluid may be pumped within a microfluidic channel across a cell/particle sensor using a microscopic resistor. The microscopic resistor may be selectively actuated so as to heat the fluid within the microfluidic channel to a temperature below a nucleation energy of the fluid so as to regulate a temperature of the fluid for at least when the cell/particle sensor is sensing the fluid.