An optical microcavity device (10), an alignment structure for an optical device, and a method for aligning an optical device are disclosed. The optical microcavity device (10) comprises: a first optical reflector (20); a second optical reflector (30) opposed to the first optical reflector (20) along an optical axis (40), the first and second optical reflectors (20, 30) being spaced from each other forming an open space therebetween; wherein the first optical reflector (20) comprises a first cavity reflector (22) and a first alignment reflector (24), wherein the second optical reflector (30) comprises a second cavity reflector (32) and a second alignment reflector (34), the second cavity reflector (32) comprising a recess to provide an optical microcavity between the first and second cavity reflectors (20, 30), the optical microcavity having an optical cavity length of at most 50 μm and/or an optical mode volume of 100 μm3 or less; an EM radiation source (50) configured for illuminating the optical microcavity with EM radiation (52) to cause multi-pass interference within the optical microcavity; and an alignment system configured to: illuminate the first and second alignment reflectors (24, 34) of the first and second optical reflectors (20, 30) to generate an optical interference pattern (74); detect the optical interference pattern (74); and determine a relative orientation and/or separation of the first and second optical reflectors (20, 30) based on the detected optical interference pattern (74); the alignment system further comprising an actuator system (100, 102) configured to move the first and second optical reflectors (20, 30) relative to each other to change the relative orientation and/or separation of the first and second optical reflectors (20, 30) based on the determined relative orientation and/or separation. At least one of the first and second alignment reflectors (20, 30) may comprise an alignment structure comprising at least two reflective surface portions having different angular orientations.

One aspect relates to a bioreactor and/or mixing container that includes an outer wall and a spectroscopy cell arranged in and/or on the outer wall. The spectroscopy cell includes a first optical area and a second optical area arranged opposite the first optical area. The first optical area and the second optical area can be set at at least two different distances from one another. A specimen-receiving area is located between the first optical area and the second optical area.

A sensor is disclosed, comprising: a first optical reflector provided on a first support element; a second optical reflector provided on a second support element and arranged opposed to the first optical reflector along an optical axis, the opposed first and second optical reflectors being spaced from each other forming a sample space for containing a sample between the first and second optical reflectors; wherein the second optical reflector comprises a recess to provide an optical cavity with stable resonance in at least one mode and having an optical cavity length of at most 50 μm and/or an optical mode volume of 100 μm.sup.3 or less; at least one electromagnetic (EM) radiation source configured to illuminate the optical cavity with EM radiation; and a detector configured to detect EM radiation from the optical cavity; wherein the first support element and the second support element are bonded to each other and form a unitary structure.

A flow cell assembly (16) for a fluid analyzer (14) that analyzes a sample (12) includes (i) a base (350) that includes a base window (350B); (ii) a cap (352) having a cap window (352B) that is spaced apart from the base window (350B); and (iii) a gasket (360) that is secured to and positioned between the base (350) and the cap (352), the gasket (360) having a gasket body (360A) that includes a gasket opening (360B). The gasket body (360A), the base (350) and the cap (352) cooperate to define a flow cell chamber (362). Moreover, an inlet passageway (366) extends into the flow cell chamber (362) to direct the sample (12) into the flow cell chamber (362); and an outlet passageway (368) extends into the flow cell chamber (362) to allow the sample (12) to exit the flow cell chamber (362).

A system for containing a sample for analysis by a spectrometer, comprising a sample receptacle unit with a well for containment of the sample, the well comprising a well inner wall and well floor, a floor aperture in the well floor, and comprising a first spectroscopy element, the first spectroscopy element spanning the opening of floor aperture, wherein the well further comprises a sealing material at the interface of the inner wall with the first spectroscopy element, wherein radiation is free to pass through the floor aperture to the first spectroscopy element.

An optical detection assembly for monitoring a biological fluid in a vessel includes two fluid-adjustment structures, which are spaced apart and configured to receive at least a portion of a biological fluid-containing vessel therebetween. A light source (which may be associated with one of the fluid-adjustment structures) is configured to emit light through a thickness of the biological fluid in the vessel, while a light detector (which may be associated with the other one of the fluid-adjustment structures) is configured to receive at least a portion of the light from the light source after it has passed through the biological fluid in the vessel. At least a portion of at least one of the fluid-adjustment structures is configured to move with respect to at least a portion of the other one so as to change the thickness of the biological fluid in the monitored portion of the vessel.

Among other things, certain embodiments of the present disclosure are related to devices and methods of performing biological and chemical assays, such as but not limited to pH measurement of bio/chemical samples.

Devices and systems are provided herein relating to a novel and rapid assay for tissue staining. Methods for using the devices and systems for analyzing tissue samples are also disclosed.

Devices, methods and systems are provided for reducing the sample volume required for analysis. Inserts placed within a sample container, and substitute sample containers having smaller volume sample chambers are provided. Methods are provided for detection and quantification of target substances in reduced volume samples. Methods include placing a small-volume of sample in a small-volume insert. Methods include diluting a small-volume sample, and placing the diluted sample in a small-volume insert. Methods include reducing the volume of sample, and: increasing illumination; increasing dye concentration or amount; increasing the amount of an enzyme substrate; increasing the amounts, concentration, or labeling of antibodies for detection; increasing optical detector sensitivity; increasing the path length of light passing through the sample; decreasing the separation between sample and detector; altering the wavelength, or polarization, or number of wavelengths, passing through the sample; increasing electronic amplification of electrical signals; altering assay temperature; and other alterations.

Devices and systems are provided herein relating to a novel and rapid assay for tissue staining. Methods for using the devices and systems for analyzing tissue samples are also disclosed.