The present disclosure relates to a speckles detection system for detecting one or more speckles on a surface of an optical element of an image capturing device. The system comprises: one or more light sources configured to emit a light beam towards the optical element, the optical element being configured to reflect light from the light sources when speckles are located on the surface of the optical element. The system further comprises one or more light receivers configured to receive the light beam reflected by the optical elements such that speckles on the surface of the optical elements are detected. Methods for detecting one or more speckles on a surface of an optical element of an image capturing device are also provided.

This disclosure relates to portable speckle imaging system and method for automated speckle activity map based dynamic speckle analysis. The embodiments of present disclosure herein address unresolved problem of capturing variations in speckle patterns where noise is completely removed and dependency on intensity of variations in speckle patterns is eliminated. The method of the present disclosure provides a correlation methodology for analyzing laser speckle images for applications such as seed viability, fungus detection, surface roughness analysis, and/or the like by capturing temporal variation from frame to frame and ignoring the intensity of speckle data after denoising, thereby providing an effective mechanism to study speckle time series data. The system and method of the present disclosure performs well in terms of time efficiency and visual cues and requires minimal human intervention.

A method for antibacterial susceptibility testing includes preparing a set of two or more samples of a plurality of bacterial cells from a patient; adding a different amount of a selected drug to each sample; illuminating at least a portion of a sample using a coherent illumination source; capturing a series of speckle images over time of at least a portion of the illuminated sample; and determining an inhibition status of the sample using a machine-learning classifier applied to the series of speckle images. The steps of illuminating the sample, capturing a series of images, and determining an inhibition status are repeated for each sample of the set of two or more samples. The method may include transforming the series of speckle images to a frequency series of speckle images; and determining the inhibition status of the sample uses the machine-learning classifier applied to the frequency series of speckle images.

Provided is a water examination device including: a main body; a fluid accommodation unit formed in the main body; a wave source for irradiating waves toward the fluid accommodation unit; a detector for detecting a laser speckle at every set time period that is set in advance, the laser speckle being generated due to multiple scattering of the waves in the fluid; a controller for estimating existence of impurities in the fluid in real-time by using the detected laser speckle; and a calibration unit for controlling the wave source or the detector such that an intensity of light irradiated from the wave source and measured by the detector is within a certain range set in advance.

One aspect of the invention relates to a method for intraoperative detection of parathyroid gland viability in a surgery, comprising obtaining speckle contrast images of a parathyroid gland of a patent; and displaying the speckle contrast images of the parathyroid gland in real-time.

Provided is a water examination device including: a main body; a cup accommodation unit formed inward from a surface of the main body such that a cup containing a fluid is accommodated therein; a wave source for irradiating a wave toward the cup accommodation unit; a detector for detecting a laser speckle generated when the irradiated wave is multiple-scattered in the fluid, at every time point set in advance; and a controller for estimating whether foreign substances exist in the fluid in real-time by using the detected laser speckle.

An observation method of a sample containing a target substance, the observation method including an imaging step in which a step of obtaining a speckle image including, as a speckle, light emitted from a luminescent substance in which a medium is brought into contact with the sample is performed a plurality of times so as to obtain a plurality of speckle images, the medium containing a probe that contains the luminescent substance emitting light and that repeatedly binds to and dissociates from the target substance directly and specifically, and an observation image generation step of generating an observation image of the target substance in the sample from the plurality of speckle images, wherein a half-life of a probe-target complex formed by binding between the probe and the target substance is equal to or more than 10 milliseconds and equal to or less than 3 seconds.

An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value K.sub.n of a speckle image I.sub.n at each time t.sub.n, determines a maximum value K.sub.max among the speckle contrast values K.sub.1 to K.sub.N, and normalizes the speckle contrast value K.sub.n at each time t.sub.n by the maximum value K.sub.max to obtain a normalized speckle contrast value K.sub.n′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value K.sub.n′ at each time t.sub.n.

A processing apparatus includes: a light source unit configured to emit partially coherent light having a coherence length that is equal to or more than inverse of a scattering coefficient of a light scattering body and shorter than half of inverse of a reduced scattering coefficient of the light scattering body; an illumination unit configured to irradiate an illumination area on a surface of the light scattering body, with the partially coherent light emitted from the light source unit; a detection unit configured to detect, in a detection area including the illumination area, a signal of scattered and returned light from the light scattering body; and an interference component extracting unit configured to extract an interference component by excluding a noninterference component from the signal of the scattered and returned light detected by the detection unit.

Additive manufacturing, such as laser sintering or melting of additive layers, can produce parts rapidly at small volume and in a factory setting. To ensure the additive manufactured parts are of high quality, a real-time non-destructive evaluation (NDE) technique is required to detect defects while they are being manufactured. The present invention describes an in-situ (real-time) inspection unit that can be added to an existing additive manufacturing (AM) tool, such as an FDM (fused deposition modeling) machine, or a direct metal laser sintering (DMLS) machine, providing real-time information about the part quality, and detecting flaws as they occur. The information provided by this unit is used to a) qualify the part as it is being made, and b) to provide feedback to the AM tool for correction, or to stop the process if the part will not meet the quality, thus saving time, energy and reduce material loss.