A sample inspection device includes a filter unit having a plurality of optical filters configured to transmit lights of different wavelength ranges, a light source unit configured to irradiate light to any one of the optical filters, a stage for placing a sample, and a reflector oriented to reflect light transmitted through one of the optical filters to the stage and to reflect light emitted from the sample on the stage to another one of the optical filters. The device also includes a detection unit for detecting light emitted from the sample on the stage through light transmitted through the another one of the optical filters, an actuator for moving the filter unit, and a control unit configured to control the actuator to move the filter unit when the detection unit receives the light passing through the another one of the optical filters.

Systems and methods include a fluorescence measurement apparatus. A single-wavelength light source generates an excitation light source. A sample holder holds a sample and includes a surface transparent to the excitation light source. Mounts attached to the single-wavelength light source(s) or the sample holder change an incident angle of the excitation light source on the surface. Optical components positioned in a path of a fluorescence emission emitted from the surface guide the fluorescence emission to a detector that obtains spectra from at least first and second angles-of-incidence. A device records spectra obtained by the detector from the first and second angles-of-incidence, normalizes and analyzes intensities of the spectra, subtracts a first spectrum corresponding to the first angle-of-incidence from a second spectrum corresponding to the second angle-of-incidence to obtain a difference, identifying a sample type of the sample based on an API gravity mapped to the difference.

An assay device is disclosed comprising a housing and a test portion, electronic circuitry and an optical assembly each a least partially located in the housing. The test portion comprises one or more test zones adapted to receive an analyte and a fluorescent label associated with the analyte, the fluorescent label being excitable by excitation light and adapted to emit emission light upon excitation by excitation light. The electronic circuitry comprises one or more light sources and one or more light detectors. The optical assembly comprises one or more excitation light guides adapted to guide excitation light from the one or more light sources to the one or more test zones, and/or one or more emission light guides adapted to guide emission light from the one or more test zone to the one or more light detectors.

A waveguide sensor system is provided. The system includes a light source and a waveguide formed from a light transmitting material. Light from the light source enters the waveguide at an input area and travels within the waveguide by total internal reflection to an analyte area and light to be analyzed travels within the waveguide from the analyte area by total internal reflection to an output area. An optical sensor is coupled to the output area and is configured to interact with the light to be analyzed. The system includes a plurality of pores located along the outer surface within the analyte area and formed in the light transmitting material of the waveguide, and the pores are configured to enhance light interaction with the analyte within the analyte area.

A system and method for detecting leakage oil with a high degree of accuracy while avoiding the complexity of the system and the influence of noise light comprise: ultraviolet light sources arranged to irradiate an oil-filled device from a plurality of different incidence angles, and are switched on and off at the respective incidence angles in sequence, and include a wavelength exciting oil; an imaging device to photograph the oil-filled device irradiated with ultraviolet light emitted from the ultraviolet light sources when the ultraviolet light sources are switched on; a recorder to record respective images photographed by the imaging device; and a display to display the respective images. The respective images are compared, a site where a light emitting position does not change always is judged as a leakage oil site, and a site emitting light or not emitting light occasionally is judged as a noise light site.

An apparatus for detecting fluorescent light emitted from a sample comprises: a light source, which is configured to emit excitation light of an excitation wavelength towards a sample comprising fluorophores such that fluorescent light is induced; a photo-detector for detecting light incident on the photo-detector; and an interference filter arranged on the photo-detector, wherein the interference filter is configured to selectively collect and transmit light towards the photo-detector based on an angle of incidence of the light towards the interference filter, wherein the interference filter is configured to selectively transmit supercritical angle fluorescence from the sample towards the photo-detector and suppress undercritical angle fluorescence from the sample.

A detection device includes a light emitting element, an accommodation frame, a light detector, and a movable light splitter. The light emitting element provides an excitation beam. The accommodation frame accommodates an object under test, and a portion of the excitation beam whose dominant light emitting wavelength falls within a first waveband range forms a fluorescent beam after passing through the object under test. The light detector receives a portion of the fluorescent beam whose dominant light emitting wavelength falls within a second waveband range. The movable light splitter forms a plurality of sub-beams from an incident beam. The sub-beams have respectively different dominant light emitting wavelengths and exits at different emitting angles. The incident beam is at least one of the excitation beam and the fluorescent beam.

Disclosed are optical trains for imaging systems. More particularly described are imaging systems configured to limit optical aberrations. Also disclosed are methods of limiting optical aberrations in imaging systems.

An assay device is disclosed comprising a housing and a test portion, electronic circuitry and an optical assembly each a least partially located in the housing. The test portion comprises one or more test zones adapted to receive an analyte and a fluorescent label associated with the analyte, the fluorescent label being excitable by excitation light and adapted to emit emission light upon excitation by excitation light. The electronic circuitry comprises one or more light sources and one or more light detectors. The optical assembly comprises one or more excitation light guides adapted to guide excitation light from the one or more light sources to the one or more test zones, and/or one or more emission light guides adapted to guide emission light from the one or more test zone to the one or more light detectors.

Various claims of a multi-laser system are disclosed. In some claims, the multi-laser system includes a plurality of lasers, a plurality of laser beams, a beam positioning system, a thermally stable enclosure, and a temperature controller. Various claims of an optical system for directing light for optical measurements such laser-induced fluorescence and spectroscopic analysis are disclosed. In some claims, the optical system includes a beam shaping element configured to generate an illumination profile having a flat top.