The present disclosure provides an automated sample analyzer having a continuous scanning optical assembly for performing an assay. The optical assembly allows for robust detection of light emitted from a reaction mixture in a dynamically changing environment, such as detection of light from a reaction mixture that is being rotated about an axis at high rotational velocity.

A device for monitoring the spatial and temporal dynamics of thrombin that includes a temperature-controlled sealed chamber with a transparent window and a light trap, the chamber being filled with a fluid medium and designed to accommodate a cuvette containing a test sample of blood plasma, and a coagulation activating insert placed into the cuvette, at least one illumination source and at least one first irradiation source and at least one second irradiation source capable of exciting a fluorescence signal of a special marker that forms in the sample during cleavage of a fluorogenic substrate, a camera, a pressure adjustment element capable of maintaining a pressure inside the chamber, the at least one first irradiation source provides irradiation of the sample in a direction perpendicular to the cuvette, and the at least one second irradiation source provides irradiation of the sample at an angle to the cuvette.

A microarray chip imaging detector comprises a housing configured to receive a microarray chip. The detector includes a laser assembly supported by the housing and oriented at an angle relative to the microarray chip, the laser assembly configured to transmit an excitation beam along a first axis to samples on the microarray chip. The detector also includes a camera supported by the housing and positioned along a second axis, the camera configured to receive fluorescent light emitted from fluorophores in the samples on the microarray chip, the second axis oriented at an angle less than 30 degrees relative to the first axis. The housing includes a plurality of baffles positioned between the microarray chip and the camera, and a plurality of laser beamstops to receive the excitation beam reflected off the microarray chip.

An optical sensor for determining the concentration of polycyclic aromatic hydrocarbons in a medium incudes a light source configured to emit transmitted light having a wavelength of less than 300 nm into the medium; a detector for receiving received light, wherein the detector is configured at least for receiving received light having a wavelength of 300 nm to 400 nm, wherein the transmitted light is converted into received light by means of fluorescence in the medium as a function of the concentration of polycyclic aromatic hydrocarbons, wherein a detector signal is generated from the received light; and a data processing unit configured to determine the concentration of polycyclic aromatic hydrocarbons using the detector signal, wherein the data processing unit controls the light source such that the light source emits modulated transmitted light according to a duty cycle. Methods of using the optical sensor are further disclosed.

A water quality analysis system is calibrated without using any organic fluorescence material such as phenanthrene. The water quality analysis system analyzes a component to be measured contained in a liquid sample by a fluorescence spectroscopy, and that comprises a light irradiation section that irradiates the liquid sample with a light having an excitation wavelength of the component to be measured, a light detection section that detects a fluorescence of the component to be measured emitted from the liquid sample, a calculation unit that calculates a concentration of the component to be measured by using a fluorescence intensity obtained by the light detection section, and a solid fluorescence reference member that is arranged on an optical path between the light irradiation section and the light detection section and that emits the fluorescence by the light from the light irradiation section at a time of calibration.

An optical sensor for determining the concentration of polycyclic aromatic hydrocarbons in a medium incudes a light source configured to emit transmitted light having a wavelength of less than 300 nm into the medium; a detector for receiving received light, wherein the detector is configured at least for receiving received light having a wavelength of 300 nm to 400 nm, wherein the transmitted light is converted into received light by means of fluorescence in the medium as a function of the concentration of polycyclic aromatic hydrocarbons, wherein a detector signal is generated from the received light; and a data processing unit configured to determine the concentration of polycyclic aromatic hydrocarbons using the detector signal, wherein the data processing unit controls the light source such that the light source emits modulated transmitted light according to a duty cycle. Methods of using the optical sensor are further disclosed.

Disclosed herein is an in-vitro method for detecting a target nucleic acid in a patient's sample. An exemplary method involves steps of bringing the sample in contact with a probe, that is a molecular beacon, is labeled with a fluorescent dye, and can hybridize with the target, then irradiating the probe with light exciting the fluorescent dye, then observing the sample with a detector, and then, if the detector detects light emitted by the probe, concluding that the target is in the sample. In order to rapidly detect the emitted light, prior to bringing the probe in contact with the sample, the probe can be immobilized in a carrier element, wherein the carrier element is in contact with an optical element which lets the emitted light pass to the detector.

The present invention relates to an apparatus for determining a concentration of a substance in a liquid medium of a test sample. The apparatus comprises a light source for directing a light beam of a first wavelength range towards the test sample, and a first detector for measuring intensity of irradiation emitted from the liquid medium at a second wavelength characteristic for the liquid medium. The apparatus further comprising a second detector for measuring intensity of irradiation emitted from the substance at a third wavelength characteristic for the substance, and a determining unit for determining the concentration of the substance in the liquid medium based on the measured intensities of the irradiation collected at the second wavelength and at the third wavelength.

Disclosed is an apparatus for determining a concentration of a substance in a liquid medium of a test sample. The apparatus comprises a light source for directing a light beam of a first wavelength range towards the test sample, and a first detector for measuring intensity of irradiation emitted from the liquid medium at a second wavelength characteristic for the liquid medium . The apparatus further comprising a second detector for measuring intensity of irradiation emitted from the substance at a third wavelength characteristic for the substance, and a determining unit for determining the concentration of the substance in the liquid medium based on the measured intensities of the irradiation collected at the second wavelength and at the third wavelength. Further, the apparatus is arranged such that the irradiation collected by the first detector is emitted from a first surface area of the test sample and the irradiation collected by the second detector is emitted from a second surface area, which first and second areas are at least partly covered by a surface area of the test sample illuminated by the light beam of the light source.

A fuel system includes a fuel line configured to allow a fuel to flow therethrough and a fluoroscopy device attached to the fuel line such that the fluoroscopy device can input excitation radiation into the fuel line and receive fluorescent radiation emitted from the fuel in the fuel line.