Patent classifications
G01N2021/653
OPTICAL ANALYSIS DEVICE AND BIOMOLECULAR ANALYSIS DEVICE
In a multi-color CARS microscope, it has been difficult to accurately bring optical axes of pump light and Stokes light into correspondence and to stably acquire a spectral signal.
Accordingly, in an optical analysis device, CARS light is generated from, a sample by using a residual component of the pump light introduced to an optical waveguide and the Stokes light generated in an optical waveguide.
Device for generating a polychromatic photon beam having substantially constant energy
Some embodiments relate to a generation device that includes: a pulsed laser source generating primary photons having at least one wavelength within pulses having time dissymmetry, a forming device(s) controlling the primary photons so as to generate a selective-polarization, focused input beam, and an optical fiber wherein the primary photons induce secondary photons having different wavelengths resulting from a raman conversion cascade and forming a wide-spectrum output beam having substantially constant energy.
Quantitative nonlinear optical microscopy using a shaped beam
A nonlinear optical microscope is provided, including source of a pulsed laser beam; a spatial light modulator for modulating the spatial profile of the pulsed laser beam; an objective for guiding the modulated beam towards a slide intended to carry a specimen; and a detector for collecting signals originating from the specimen, wherein the spatial light modulator is designed to modulate the intensity and/or the phase of the pulsed laser beam on the rear pupil of the objective to produce a beam that is axially extended and confined in one or two lateral directions after focusing by the objective, and wherein the slide is placed on a motorized stage of a histology slide scanner assembly.
Methods and apparatuses for label-free particle analysis
An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles.
Optical detection device, optical detection method, and program
A phase sensitive detection mechanism that uses electrical processing is realized, and an optical detection device, an optical detection method, and a program that are capable of detecting faint light at high speed and with high sensitivity are provided by a simple configuration. A light source section generates a first pulsed light. A filter section transmits a second pulsed light formed from a portion of a frequency spectrum exhibited by the first pulsed light, and reflects a third pulsed light formed from another portion of the frequency spectrum exhibited by the first pulsed light. A phase modulation section phase modulates the second pulsed light at plural phases. A multiplexing section produces a fourth pulsed light by multiplexing the third pulsed light with the second pulsed light phase modulated by the phase modulation section. A detector spectrally disperses and detects scattered light generated by radiating the fourth pulsed light onto a target object. An extraction section uses specific calculation processing to synchronize with the phase modulation in the phase modulation section, so as to extract a frequency spectrum of scattered light scattered based on the second pulsed light phase modulated by the phase modulation section from the frequency spectrum of the scattered light detected by the detector.
SYSTEM AND METHOD FOR PERFORMING A PHOTOLUMINESCENCE ANALYSIS ON A MEDIUM
Systems and methods for performing a photoluminescence analysis on a medium such as the fundus of a patient's eye are provided. An imaging device, a spectral analyser and an excitation light source are provided. An optical assembly defines an imaging light path between the patient's eye and the imaging device and a spectral analysis light path between an analysis spot on the patient's eye and the spectral analyser. The excitation light source generates an excitation light beam comprising an excitation wavelength selected to excite components present in the patient's eye for the generation of fluorescent light at a photoluminescence wavelength, and is optically coupled to the spectral analysis light path for projecting the excitation light beam on the analysis spot. An optical filter is coupled to the spectral analyser, and has a low light transmissivity at the excitation wavelength and a high light transmissivity at the photoluminescence wavelength.
HYPERSPECTRAL NONLINEAR MICROSCOPY
In an example embodiment, a method includes emitting broad bandwidth radiation with high spatial coherence. The method includes applying a time-varying modulation to the broad bandwidth radiation. The method includes identifying optical interactions caused by the time-varying modulation of the broad bandwidth radiation. The method includes identifying one or more signals included in the optical interactions. The method includes extracting one or more respective spectral signatures associated with each respective signal of the one or more signals. The method includes determining a respective characteristic of an optically interacting material that corresponds to a respective spectral signature of the extracted spectral signatures. The method includes identifying one or more optically interacting materials by classifying one or more of the characteristics.
Generating synchronized laser pulses at variable wavelengths
The invention relates to an apparatus for generating laser pulses. It is an object of the invention to provide a method for generating synchronized laser pulse trains at variable wavelengths (e.g., for coherent Raman spectroscopy/microscopy), wherein the switching time for switching between different wavelengths should be in the sub-μs range. For this purpose the apparatus according to the invention comprises a pump laser (1), which emits pulsed laser radiation at a specified wavelength, an FDML laser (3), which emits continuous wave laser radiation at a cyclically variable wavelength, and a nonlinear conversion medium (4), in which the pulsed laser radiation of the pump laser (1) and the continuous wave laser radiation of the FDML laser (3) are superposed. In the nonlinear conversion medium (4) the pulsed laser radiation of the pump laser (1) and the continuous wave laser radiation of the FDML laser (3) are converted in an optical parametric process into pulsed laser radiation at a signal wavelength and an idler wavelength that differs therefrom. Furthermore the invention relates to a method for generating laser pulses.
TOTAL INTERNAL REFLECTION ENABLED WIDE-FIELD COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY
A system is provided. The system has a femtosecond oscillator to generate pulses used for pump and probe beams. A photonic crystal fiber is disposed in a path of the probe beam and produces pulses for a chirped probe beam. A high NA objective receives the pump and the chirped probe beam, redirects the received beams through a dielectric substrate towards an interface between a sample and the dielectric substrate to cause total internal reflection (TIR) at the sample-substrate interface, and produces corresponding evanescent waves in a portion of the sample adjacent to the sample-substrate interface, and collects a backward-propagating beam of pulses of responsive light. The portion of the sample illuminated by the evanescent waves emits responsive light. The dielectric substrate is transparent to the responsive light, the pump and the chirped probe beam. An image is produced having a specific image size using the received backward-propagating beam.
Use of vibrational spectroscopy for microfluidic liquid measurement
An apparatus for sorting cells includes a measurement volume that contains a cell to be measured, a light source that provides light to cause an emission by a fluorescent label attached to the cell, and an optic device that directs the light through the measurement volume. The apparatus flows the cells through the measurement volume such that as the cell flows through the measurement volume, it interacts with the light, resulting in a change in light originating from the measurement volume, the change in light is a fluorescence emission. Another optic device directs a portion of the light originating from the measurement volume to a detector, which detects the portion of the light. A processor operably coupled to the detector generates an estimate of DNA quantity in the cell based on the change in light originating from the measurement volume, and determines a characteristic of the cell from the estimate.