A Raman spectrometric imaging method, including: placing a sample on a two-dimensional translation stage; emitting a first light beam by a first optical comb light source; dividing the first light beam into a pump light beam and a depletion light beam to illuminate the sample; guiding the pump light beam to illuminate a region of the sample to excite molecules of the sample in the region; guiding the depletion light beam to the region of the sample to make excited molecules at a periphery of the region to return into a vibrational ground state; emitting a second light beam as a probe light beam by a second optical comb light source to the remaining excited molecules to generate a CARS signal; recording the CARS signal for imaging; moving the two-dimensional translation stage to scan other regions of the sample to form an image of the sample.

Object: To provide a remote substance identification device that can identify an unidentified substance, such as a harmful substance, from a remote location. Solution: Provided are a remote substance identification device and method, the device comprising a laser device 10 that emits a laser beam to an irradiated space; a wavelength conversion device 20 that converts a wavelength of the laser beam emitted from the laser device into a plurality of different wavelengths and that emits laser beams of the different wavelengths to the irradiated space; a light collecting-detecting device 30, 40, 50 that collects and detects resonance Raman-scattered light generated from an irradiated object due to resonance Raman scattering; and a processor 60 that identifies the irradiated object on the basis of a result detected by the collecting-detecting device 30, 40, 50.

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

A system for measurement is provided. The system includes a scanning interface module for placement on a skin for non-invasive scanning. The scanning interface module includes a cavity to be pressed on the skin to make a vertical portion in the cavity, and a nonlinear optical system for scanning the vertical portion via an input side and an output side of the cavity that are laterally facing each other.

According to exemplary embodiments of the present disclosure, it is possible to provide method, system, arrangement, computer-accessible medium and device to stimulate individual neurons in brain slices in any arbitrary spatio-temporal pattern, using two-photon uncaging of photo-sensitive compounds such as MNI-glutamate and/or RuBi-Glutamate with beam multiplexing. Such exemplary method and device can have single-cell and three-dimensional precision. For example, by sequentially stimulating up to a thousand potential presynaptic neurons, it is possible to generate detailed functional maps of inputs to a cell. In addition, it is possible to combine this exemplary approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity. Further exemplary embodiments of the present disclosure can include a light-weight, compact portable device providing for uses in a wide variety of applications.

In an embodiment an apparatus includes at least one optoelectronic laser configured to provide excitation radiation to a sample, the excitation radiation being generated by an electric current flowing through the at least one optoelectronic laser, a transistor configured to modulate the electric current flowing through the at least one optoelectronic laser in order to switch on and off generation of the excitation radiation and a spectrometer configured to analyze Raman light scattered from the sample in response to exposing the sample to the excitation radiation, wherein the Raman light includes one or more spectral components, wherein the spectrometer includes a diffraction element configured to split the Raman light into the spectral components, and wherein the diffraction element includes at least a photonic crystal or a plasmonic Fabry Perot filter.

A method for coherent multidimensional spectroscopy may comprise illuminating a location in a sample with a set of m coherent light pulses, each coherent light pulse having an initial frequency ω.sub.m and an initial wave vector {right arrow over (k)}.sub.m, wherein m≥2, to generate a coherent output signal having an initial frequency ω.sub.output=Σ±ω.sub.m and an initial wavevector wave vector {right arrow over (k)}.sub.output=Σ±{right arrow over (k)}.sub.m; scanning a first coherent light pulse of the set of m coherent light pulses across a set of i frequency values, wherein i≥2, the set of i frequency values including the first coherent light pulse having initial frequency ω.sub.1; scanning, simultaneously, a second coherent light pulse of the set of m coherent light pulses across a set of i correlated frequency values, the set of i correlated frequency values including the second coherent light pulse having initial frequency ω.sub.2, wherein each correlated frequency value is associated with a corresponding frequency value of the set of i frequency values as a correlated frequency grouping; and detecting the coherent output signal. Each correlated frequency value is selected so that the coherent output signal generated at each correlated frequency grouping equals the initial frequency ω.sub.output and the coherent output signal generated at each correlated frequency grouping equals the initial wavevector {right arrow over (k)}.sub.output.

Provided is a Raman-active nanoparticle including: a spherical plasmonic metal core; a plasmonic metal shell having surface irregularities; and a self-assembled monolayer which binds to each of the core and the shell, is positioned between the core and the shell, and includes a Raman reporter satisfying the following Chemical Formula 1:

NO.sub.2—Ar—SH  (Chemical Formula 1)

wherein Ar is a (C6-C12) arylene group.

A system for multimodal nonlinear optical imaging is provided. Each mode uses a high NA objective to cause total internal reflection excitation at a sample-substrate interface. The system has a femtosecond oscillator to generate pulses used for two beams. The objective receives at least one beam, redirects the received at least one beam through a dielectric substrate to cause the TIR and produces corresponding evanescent waves in a portion of the sample adjacent to the sample-substrate interface, and collects a backward-propagating beam of pulses of responsive light. The portion of the sample illuminated by the evanescent waves emits responsive light. Different modes or combinations of the distinct modalities may be selected to access complementary chemical and structural information for various chemical species near the sample-substrate interface. Each mode may have mode-specific control such as selective beam blocking, power ratios and filtering.

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.