Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

Systems and methods are provided for a UV-VIS spectrophotometer, such as a UV-VIS detector unit included in a high-performance liquid chromatography system. In one example, a system for the UV-VIS detector unit may include a first light source, a signal detector, a flow path positioned intermediate the first light source and the signal detector, a second light source, and a reference detector. The first light source, the signal detector, and the flow path may be aligned along a first axis, and the second light source and the reference detector may be aligned along a second axis, different than the first axis.

A detector (50) for a liquid chromatograph includes a tube (56) and a flow cell (52) arranged so that a mobile phase and a sample exiting from a column (10) in a liquid chromatograph (100) flow through the tube into the flow cell which is configured to allow for detection of a component in a sample flowing within the flow cell. The tube includes a first wetted member (56) made of a PEEK resin material. The flow cell has a passage surface formed by a second wetted member (521) including a non-metallic material having a lower electric resistivity than the first wetted member. The use of those wetted members facilitates the discharging of electric charges from the flow cell while preventing adsorption of sample components to the inner surface of the flow cell.

An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Apparatuses and methods for whole column imaging detection (WCID) capillary isoelectric focusing (CIEF). The apparatus includes a separation capillary having a separation inner diameter and a separation outer diameter; a base, wherein the separation capillary is anchored to the base; an inlet transfer capillary having an inlet inner diameter and an inlet outer diameter; and an outlet transfer capillary having an outlet inner diameter and an outlet outer diameter. The inlet transfer capillary, the separation capillary, and outlet transfer capillary are configured to be in fluidic communication with each other. The separation inner diameter exceeds the outlet inner diameter.

Apparatuses and methods for whole column imaging detection (WCID) capillary isoelectric focusing (CIEF). The apparatus includes a separation capillary having a separation inner diameter and a separation outer diameter; a base, wherein the separation capillary is anchored to the base; an inlet transfer capillary having an inlet inner diameter and an inlet outer diameter; and an outlet transfer capillary having an outlet inner diameter and an outlet outer diameter. The inlet transfer capillary, the separation capillary, and outlet transfer capillary are configured to be in fluidic communication with each other. The separation inner diameter exceeds the outlet inner diameter.

An apparatus includes a flow cell body, a plurality of electrodes, an imaging assembly, and one or more barrier features. The flow cell body defines one or more flow channels and a plurality of wells defined as recesses in the floor of each flow channel. Each well is fluidically coupled with the corresponding flow channel. The flow cell body further defines interstitial surfaces between adjacent wells. Each well defines a corresponding depth. Each electrode is positioned in a corresponding well of the plurality of wells. The electrodes are to effect writing of polynucleotides in the wells. The imaging assembly is to capture images of polynucleotides written in the wells. The one or more barrier features are positioned in the wells, between the wells, or above the wells. The one or more barrier features contain reactions in each well, reduce diffusion between the wells, or reduce optical cross-talk between the wells.

A chromatography detector comprises: a flow cell with a flow path where the sample and a solution flow, the flow cell including outlet tubing that discharges the sample and the solution from the flow path; a connecting member configured to connect the outlet tubing of the flow cell and external tubing outside the flow cell; a wall member including a through-hole; a securing member configured to secure the connecting member to the wall member; and a tray below the securing member. The securing member includes a holder configured to hold the connecting member, an attachment configured to be inserted through the through-hole of the wall member, and a fluid guide formed in such a manner as to, upon the solution leaking out of the connecting member, guide the leaked solution to the tray.

A chromatography detector comprises: a flow cell with a flow path where the sample and a solution flow, the flow cell including outlet tubing that discharges the sample and the solution from the flow path; a connecting member configured to connect the outlet tubing of the flow cell and external tubing outside the flow cell; a wall member including a through-hole; a securing member configured to secure the connecting member to the wall member; and a tray below the securing member. The securing member includes a holder configured to hold the connecting member, an attachment configured to be inserted through the through-hole of the wall member, and a fluid guide formed in such a manner as to, upon the solution leaking out of the connecting member, guide the leaked solution to the tray.