The present disclosure discusses a method of separating and/or purifying acidic peptides by the use of a mobile phase having a pH greater than or about equal to the isoelectric point of one or more of the metal oxides in the flow path.

Methods for determining the relative abundance of viral capsid components in a sample of viral particles are disclosed. In embodiments, methods for determining the relative abundance of empty capsids, partially-full capsids and full capsids (e.g., containing a heterologous nucleic acid molecule) of adeno-associated virus are disclosed.

The invention discloses a detection method based on supercritical fluid chromatography (SFC) and post-column dicationic ionic liquid (DIL) charge complexation, which includes the following steps: (1) The supramolecular solvent (SUPRAS) was prepared by mixing heptanol, tetrahydrofuran, and water; (2) Sample pretreatment: the SUPRAS was used to extract the sample for subsequent analysis; (3) Analysis of perfluorinated compounds (PFCs) using SFC separation, post-column DIL-based charge complexation, and electrospray ionization-mass spectrometry (ESI-MS). The invention established a novel analytical method for the detection of PFCs in textiles incorporating post-chromatographic DIL-based charge complexation and SFC coupled with ESI-MS. The DIL reagent formed positively charged complexes with anionic analytes during the ESI process, facilitating MS detection in the positive ion mode with enhanced detection sensitivity.

Systems and methods that facilitate the automatic (or substantially automatic) preparation of a sample of a product containing molecules for analysis and automatic (or substantially automatic) performance of an assay of that sample. Thus, the preparation and analysis can be performed substantially in-real time, or, in other words, much more quickly than presently allowed by conventional systems and methods.

An odor evaluation device, including: a gas chromatograph including a separation column configured to temporally separate a plurality of components contained in an odoriferous analysis-target gas; a timing detector configured to detect, for each of the plurality of components, a timing at which the component exits from the separation column; a gas collector configured to collect, into a sample bag, a gas containing all or some of the components exiting from the separation column when the analysis-target gas is passed through the separation column; a timing setter configured to allow a setting of a timing at which a component contained in the gas to be collected into the sample bag exits from the separation column; and a gas introducer configured to introduce a dilution gas into a passage connecting the separation column and the sample bag.

A gas separation system includes a separation column for separating components contained in sample gas, a sample gas supplier fluidly connected to an inlet of the separation column for supplying sample gas to the separation column, a detector fluidly connected to an outlet of the separation column, a collection tube filled with an adsorbent having a property of adsorbing a target component in the sample gas under a condition of a first temperature or less and desorbing the adsorbed target component under a condition of a second temperature or more higher than the first temperature, a temperature adjuster for adjusting a temperature of the collection tube, a collection container for collecting the target component, and a switching mechanism for switching between a state in which the collection tube is connected to an outlet of the detector and a state in which the collection tube is connected to the collection container.

Methods and apparatus for controlling interdetector band broadening during the analysis of a sample injected into a chromatography system. A column flow is diluted with a dilution flow after the sample exits the chromatography system, and the diluted sample is analyzed by one or a combination of analysis instruments such as a light scattering detector, refractive index detector, an ultraviolet absorption detector.

A monitoring method of dissolved greenhouse gases in wastewater includes: S1, collecting a wastewater sample, performing mud-water separation, and collecting a supernatant after the mud-water separation; S2, adding dilute sulfuric acid solution to the supernatant collected in a headspace vial to adjust pH of the supernatant to 1-4, and then tightening a cap of the headspace vial; S3, inverting the headspace vial, and checking whether there are air bubbles in the headspace vial; S4, injecting 5-10 mL of pure nitrogen into the headspace vial through a syringe, and discharging 5-10 mL of the wastewater sample through a conduit; S5, placing the headspace vial in a water bath constant temperature shaker, and shaking the headspace vial for 20-30 minutes; S6, extracting gases from an upper part of the headspace vial, and measuring concentrations of the gases; S7, quantitatively calculating concentrations of the dissolved greenhouse gases in the wastewater sample.

Systems and methods that facilitate the automatic (or substantially automatic) preparation of a sample of a product containing molecules for analysis and automatic (or substantially automatic) performance of an assay of that sample. Thus, the preparation and analysis can be performed substantially in-real time, or, in other words, much more quickly than presently allowed by conventional systems and methods.