A mobile phase monitor includes a measurement unit, an arithmetic unit, a storage, and a discrimination unit. The measurement unit measures a weight of a mobile phase container. The arithmetic unit produces a calibration curve indicating a relationship between a measurement value of the measurement unit and a liquid amount of the mobile phase accommodated in the mobile phase container. The arithmetic unit calculates the liquid amount of the mobile phase from the measurement value of the measurement unit based on the produced calibration curve. The storage stores a plurality of calibration curves respectively corresponding to a plurality of types of mobile phases. The discrimination unit discriminates a type of the mobile phase accommodated in the mobile phase container by searching for the produced calibration curve from the plurality of calibration curves stored in the storage.

A method of performing mass spectrometry includes accumulating, over an accumulation time, ions produced from components eluting from a chromatography column and transferring the accumulated ions to a mass analyzer. During an acquisition, a mass spectrum of detected ions derived from the transferred ions is acquired. An elution profile is obtained from a series of acquired mass spectra including the acquired mass spectrum and a plurality of previously-acquired mass spectra. The elution profile includes a plurality of detection points representing intensity of the detected ions as a function of time. A current signal state of the elution profile is classified based on a subset of detection points included in the plurality of detection points. The accumulation time for a next acquisition of a mass spectrum is set based on the classified current signal state of the elution profile.

The disclosure includes an intelligent automatic control system for mine gas chromatographs, comprising a CPU. The system may comprise a touch screen coupled to the CPU, a computer and a relay unit electrically coupled to the CPU, and a remote transmission module and a remote mobile control terminal communicatively coupled to the CPU. A digital output terminal may be electrically coupled through the relay unit to a component selected from the group consisting of a solenoid valve, at least one heater, a chromatograph motor, a six-way injection valve, a ten-way injection valve, a chromatograph automatic injection pump, FID ignition coils, a TCD bridge solenoid valve, at least one gas generator solenoid valve, and a standard gas/sample gas conversion valve. The system may comprise at least one temperature sensor, at least one gas pressure sensor, a TCD bridge module, and at least one pressure-controlling switch electrically coupled to the CPU.

Disclosed herein are chromatography instrument support systems, as well as related apparatuses, methods, computing devices, and computer-readable media. For example, in some embodiments, a chromatography instrument support apparatus may include: first logic to generate one or more peak locations for a chromatogram data set and to generate one or more baselines for the chromatogram data set, wherein an individual peak has an associated baseline, and wherein the first logic includes a machine-learning computational model that outputs estimated peak locations and estimated baselines; second logic to cause the display of the one or more peak locations and the one or more baselines concurrently with the display of the chromatogram data set; and third logic to, for individual peaks, generate an associated integrated value representing an area above the associated baseline and under a portion of the chromatogram data set corresponding to the individual peak.

A container assembly for use with a high-pressure liquid chromatography (HPLC) instrument is disclosed, in which the container assembly, when coupled to a source of pressurized gas, provides fluid medium to the HPLC instrument at positive pressure. The container assembly has an external exterior container shell, an internal fluid container for holding fluid medium, an interstitial volume between the external exterior container shell and the internal fluid container, a port for fluidly connecting the volume to a pressurized gas source, and a port for fluidly connecting the internal fluid container to the HPLC instrument. As a pressurized gas in the interstitial volume increases, fluid medium flows out of the port connected to the internal fluid bag and container assembly at a positive pressure. A system incorporating the container assembly, and method of use of the same, are also disclosed.

In a quantitative determination device 10 for brominated flame-retardant compounds, a storage section 41 holds a relative response factor 411 representing a relationship of a measured intensity of a compared compound to that of a reference compound selected from target compounds. A standard-sample measurer 43 acquires the intensity of the reference compound by measuring a standard sample, using an analyzer 10, 20. A target-sample measurer 45 acquires the intensities of the reference and compared compounds by measuring a target sample, using the analyzer. A reference-compound quantity determiner 46 determines a quantitative value of the reference compound in the target sample. A compared-compound quantity determiner 47 determines a quantitative value of the compared compound based on the quantity of the reference compound in the standard sample, intensity of the reference compound acquired by the standard-sample measurer, intensity of the compared compound acquired by the target-sample measurer, and relative response factor of the compared compound.

[Problem to be solved] To provide a method for analyzing amino acids capable of easily analyzing D/L-amino acids in a sample with high reproducibility, particularly a simultaneous analytical method for L-amino acids and D-amino acids constituting a protein.

[Solution] A method for analyzing amino acids by liquid chromatography, in which a sample containing a plurality of kinds of amino acids is derivatized with a derivatization reagent, and the obtained derivatized sample is circulated on a column together with a mobile phase, wherein the mobile phase is composed of a plurality of mobile phases, and at least one mobile phase is a mixed solvent system, wherein two or more kinds of derivatized samples are prepared using two or more kinds of derivatization reagents, wherein different analytical conditions in which mixing ratio of the plurality of the mobile phases is changed with a passage of time are set for each kind of the derivatization reagent, and a solvent mixing ratio in the mobile phase being the mixed solvent system is set for the each kind of the derivatization reagent, and wherein the two or more kinds of derivatized samples are analyzed by automatically switching between the different analytical conditions and the solvent mixing ratio to separate and quantify derivatized L-amino acids and derivatized D-amino acids.

In an automatic analyzer where a plurality of types of separation column are selectively used for performing a chromatographic analysis, an automatic analyzer configured to install a separation column of an intended type at an aimed position, and a method for installing the separation column in the automatic analyzer are provided. Identification mechanisms, a computer, and a display unit are included. The identification mechanisms read identifiers included in separation columns when the separation columns are installed at installation positions. The computer determines whether identification information of the identifiers read by the identification mechanisms matches an installation pattern registered in advance or not. The display unit notifies an alarm when the identification information is determined not to match the installation pattern by the computer.

A gas component detection device includes a column, a sensor, a flow path, and a flow path switch. The column separates a component of gas to be detected. The sensor is, for example, a semiconductor sensor, is connected to the downstream side of the column, and detects a component of gas to be detected. The flow path connects the column and the sensor. The flow path switch is arranged between a flow path and a flow path. The flow path switch switches between and executes a measurement mode in which gas to be detected discharged from the column flows into the sensor, and a discharge mode in which gas to be detected discharged from the column is discharged to the outside.

An operational condition of a liquid chromatography (LC) system (2110) is detected and displayed without user intervention. A plurality of pressure measurements over time are received from a pressure sensor (2119) of the LC system. A processor (2140) calculates values from the measurements for six parameters including a beginning pressure (P.sub.B), an ending pressure (P.sub.E), an average pressure (T.sub.1) for a first half of the separation, an average pressure (T.sub.2) for a second half of the separation, a ratio T.sub.1/P.sub.B, and a ratio T.sub.2/P.sub.B. The values of the six parameters are classified as one of one or more operational conditions of the LC system using a machine learning model. The machine learning model is created from values of the six parameters calculated from known separations for each of the one or more operational conditions. The operational condition found from the classification is displayed on a display device (2141).