An analysis device having a liquid chromatograph prevents a plurality of streams from being unusable at the same time Whether a usable stream is 0 or not among streams 1, 2, and 3 is determined. In a case where none of the streams is usable, the control unit skips a sample introduction in the cycle; and where there is one usable stream in the cycle, the stream is used. In a case where there are multiple streams that are usable in the cycle, and there are multiple streams of which the remaining number of uses of a separation column is the minimum, the stream having the smallest stream number is used; and where there is one stream of which the remaining number of uses of a separation column is the minimum, the stream to which the separation column having the minimum remaining number of uses is connected and used.

A temperature control chamber includes a heat impact unit configured for heat impacting a sensor unit in accordance with a heat profile, the sensor unit configured for sensing heat impact response data over time in response to being heat impacted with the heat profile, and a determining unit configured for determining information indicative of a heat transfer characteristic in the temperature control chamber based on the sensed heat impact response data.

A method for scientific instrument support includes obtaining a chromatographic data. The method includes one of (a) calculating an intensity score for the chromatographic data and identifying an injection miss when the intensity score is below a score threshold or (b) applying a machine learning model to classify the chromatographic data. The method further includes notifying a user of an injection miss or injection error.

The disclosure provides systems and methods useful for predicting or detecting a malfunction in a chromatography process in real-time. In some embodiments, the disclosure provides systems and methods for detecting an atypical profile in a process chromatogram in ion-exchange chromatography of a biologic product.

Techniques and apparatus for evaluating analytical device performance and data quality are described. In one embodiment, for example, an apparatus may include at least one memory, and logic coupled to the at least one memory. The logic may be configured to generate an analysis method to be performed by an analytical device, the analysis method comprising a plurality of method segments comprising at least one performance assessment process and at least one sample analysis process, and link the at least one performance assessment process with the at least one sample analysis process. Other embodiments are described.

A chromatography system includes a gradient delay volume defined as an overall fluid volume between where gradient is proportioned until an inlet of a chromatography column, a pump pumping a flow of gradient; and at least one valve located downstream from the pump, the at least one valve having a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the at least one valve, the at least one valve having at least two positions. A first position of the at least two positions of the at least one valve increases the gradient delay volume of the chromatography system relative to when the at least one valve is in a second position.

To make it easy to address the case in which a chromatograph does not appropriately operate. A chromatograph (liquid chromatograph 100) for analyzing a sample by supplying an eluent and the sample and separating a component contained in the sample to detect the component, the chromatograph including: a detection portion (controller 170) configured to detect a fault in the analysis; and an operation controller (controller 170) configured to cause a constituent element related to the analysis to perform at least one of an operation for identifying a factor of the fault and an operation for avoiding the fault.

Certain embodiments of the invention provides a method for monitoring level of saturation of a chromatography media in a column, which method comprises measuring a first pressure at the inlet of an unloaded column; measuring a second pressure at the inlet from a loaded column; and comparing the first and second pressure measurement to determine the level of saturation of the chromatography media. Embodiments of the invention also provide related methods for controlling a chromatography system and methods for controlling a periodic counter current chromatography system, as well as a chromatography system suitable for use with the novel methods.

Embodiments of the present disclosure are directed to methods and systems for assessing integrity of chromatography columns, systems, and processes. The methods and systems can comprise one or more of extracting a block and signal combination for analysis, performing a transition analysis, performing one or more statistical process controls, and/or implementing in-process controls based on the statistical process controls.

The present invention relates to a method for operating a chromatography column comprising (a) providing a first value of a lifetime (first lifetime value) of said chromatography column; (b) performing a chromatographic separation of a sample on said chromatography column; (c) providing a value of a weighted aging factor determined based on at least one aging parameter selected from sample type, sample dilution, and sample volume; and (d) determining a second value of said lifetime (second lifetime value) of said chromatography column based on said first lifetime value and said weighted aging factor. The present invention also relates to further methods, databases, devices, and uses related thereto.