A method of performing a thin layer chromatography comprises a step of providing a three-dimensional machine configured to move a rigid nozzle under control by a computer along a stationary adsorbent layer, the nozzle combines the ends of individual tubing carrying individually-controlled flows of eluent components, a step of individually operating a plurality of pumps for pumping individual eluent components through the respective tubing towards the rigid nozzle, a step of operating the three-dimensional machine for moving the rigid nozzle adjacent and along the outer surface of the adsorbent layer while continuing to pump individual eluent components, a step of operating a camera connected to the computer to observe a migration of the eluent front on the adsorbent layer, and a step of individually adjusting flow rates of individual pumps by the computer using a dynamic position of the eluent front as observed by the camera.

A derivatization apparatus and method for coating a sample carrier with a reagent solution. The derivatization apparatus consists of a closed system in which a small quantity of reagent solution is sprayed into a closed container via a spray nozzle. A sample carrier is located in the closed container and is coated as homogeneously as possible with the reagent solution.

A chromatographic medium having a separating agent layer, which is used to separate target substances, a filling agent layer, which is used to fix the target substances before the target substances are separated, and a permeation layer, which is used to enable permeation of the target substances separated by the separating agent layer, wherein the filling agent layer comes into contact with the separating agent layer via a plane that intersects the direction of development of the target substances in the chromatographic medium and is positioned on the upstream side in the direction of development, the separating agent layer exhibits separability of the target substances and optical responsiveness to ultraviolet rays, and the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber 1 in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase.

A method for simulataneously separating and detecting aldehydes or ketones from a plurality of samples containing the samein a simple and rapid manner by using a rotary microdevice capable of integrating derivatization and TLC separation of aldehydes and ketones, and the method providing reliable TLC separation, control of moving speed of an eluent on a TLC plate, and improved TLC resolution.

A method for screening adulteration of fibrate anti-hyperlipidemia chemicals in tea by combined method of high performance thin layer chromatography (HPTLC) and bioluminescence, which belongs to the field of food inspection. The method includes: firstly, formulating standard solutions of bezafibrate and ciprofibrate, and preparing a tea sample; pre-washing a thin-layer plate and then performing HPTLC spotting; performing HPTLC separation to move original mixed target objects mixed originally onto different positions of the thin-layer plate according to different molecular structures, so as to form a physical isolation; and subsequently, simultaneous detection of multiple targets in the tea sample could be realized conveniently through luminous bacteria coupled with the thin-layer plate by an immersed manner. The present disclosure establishes a method capable of detecting anti-hyperlipidemia chemicals in tea rapidly and quantitatively by the combined detection method of HPTLC and bioluminescence, which has the advantages of being economic, rapid, simple and convenient.

(EN) In the chromatographic chamber (3), to which the tip (11) enters from below. The tip (11) serves as the end of separate supply lines (5a, 5b, 5c . . . 5x), and each supply line (5a, 5b, 5c . . . 5x) is intended to deliver a separate eluent component. The first supply line (5a) comprises the first reservoir (6a) connected to the first pump (7a), to which the first flexible tube (8a) is connected terminated with the rigid tube (9a). The tip (11) is the first turning point (21) and then, with the use of the three-dimensional machine (2), it is passed along the line to the second turning point (22) and back again, while the individual components are pumped with variable efficiency controlled by the computer (20). This results in a quantitative and qualitative composition in time. At the same time, the position of the moving front is registered with the digital camera (19), and the signals of the eluent front migration distance are registered by the computer (20), and based on this information, the pumps (7a, 7b . . . 7x) that the individual components of the eluent are controlled accordingly. After reaching the final migration of the eluent front, the delivery of the components is stopped, and then the plate (18) is removed from the chromatographic chamber (3) and dried under the hood. As a result, the developed chromatogram is obtained. (19), and the signals of the eluent front migration distance are registered by the computer (20), and based on this information, the pumps (7a, 7b . . . 7x) that deliver individual components of the eluent are controlled accordingly. After reaching the final migration of the eluent front, the delivery of the components is stopped, and then the plate (18) is removed from the chromatographic chamber (3) and dried under the hood. As a result, the developed chromatogram is obtained. (19), and the signals of the eluent front migration distance are registered by the computer (20), and based on this information, the pumps (7a, 7b . . . 7x) that deliver individual components of the eluent are controlled accordingly. After reaching the final migration of the eluent front, the delivery of the components is stopped, and then the plate (18) is removed from the chromatographic chamber (3) and dr

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber 1 in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase.

A method for the automatic chromatography of thin-layer plates for thin-layer chromatography with a development chamber 1 in which a thin-layer plate D is completely enclosed, sealed-off and isolated from the external environment. In the development chamber, a front space containing an inner atmosphere is located on the front face of the separation layer of the thin-layer plate. The depth of the front space is about 2 mm and a maximum 3 mm. An inlet is provided at one end of the front space and an outlet is provided at the other end of the front space. During the chromatographic development, a stream of gas of particular composition determined by the user is created throughout the entire front space, the entire inner atmosphere being set in motion, without stagnant or stationary gas phase.

The present invention discloses an assay plate, which has a plate body made of polymeric material modified by coating a compound A thereon, and allows a molecule such as protein or peptide, or a group to bind to the plate body by hydrophobic bonding for use in biomedical assay.