Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

Double sided flow cell and other substrate imaging systems, such as imaging systems used in nucleic acid sequencing and similar processes. In one example, the imaging system includes a flipper to facilitate imaging different surfaces of the flow cell or other substrate. In another example, the imaging system includes two optical systems for imaging different surfaces of the flow cell or other substrate. In another example, the imaging system is an immersion system. In these and other examples, the system may include an auto-focus sub-system configured to accurately focus the optics on one surface of the double sided flow cell without interference from the other surface of the flow cell.

Double sided flow cell and other substrate imaging systems, such as imaging systems used in nucleic acid sequencing and similar processes. In one example, the imaging system includes a flipper to facilitate imaging different surfaces of the flow cell or other substrate. In another example, the imaging system includes two optical systems for imaging different surfaces of the flow cell or other substrate. In another example, the imaging system is an immersion system. In these and other examples, the system may include an auto-focus sub-system configured to accurately focus the optics on one surface of the double sided flow cell without interference from the other surface of the flow cell.

A flow cell of the flow cytometer of the present invention includes: a sample flow path through which a sample fluid containing a sample flows; and a sample fluid supply portion which communicates with an upstream end of the sample flow path in the sample fluid flow direction and supplies the sample fluid to the sample flow path, wherein the sample fluid supply portion includes a plurality of sample opening portions which supply a sample fluid to the sample flow path, a cleaning liquid supply opening portion to which a second tube is connectable and which supplies a cleaning liquid for cleaning the sample fluid supply portion, and a cleaning liquid discharge opening portion to which a first tube is connectable and which discharges the cleaning liquid from the sample fluid supply portion.

This invention relates to retrievable measuring cell for optical measurements in gas, the cell being defined by a gas conducting pipe having an input end adapted to be connected to a gas flow input intruding gas into the cell and an output end adapted to be connected to a gas flow output. The pipe ends also being adapted to be coupled to optical components including an optical transmitter transmitting light into said cell and an optical receiver adapted to receive light having passed through said cell, the optical beam in said cell having a predetermined shape, the optical components including a light source, at least two minors and a light receiver being mounted in known positions on an external frame covered by the pipe ends. The cell has an elongated shape corresponding to the optical beam shape.

This invention relates to retrievable measuring cell for optical measurements in gas, the cell being defined by a gas conducting pipe having an input end adapted to be connected to a gas flow input intruding gas into the cell and an output end adapted to be connected to a gas flow output. The pipe ends also being adapted to be coupled to optical components including an optical transmitter transmitting light into said cell and an optical receiver adapted to receive light having passed through said cell, the optical beam in said cell having a predetermined shape, the optical components including a light source, at least two minors and a light receiver being mounted in known positions on an external frame covered by the pipe ends. The cell has an elongated shape corresponding to the optical beam shape.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting and analyzing blood cells in biological samples. A small measured quantity of a biological sample, such as whole blood, is placed in a mixing bowl on the disposable test cartridge after being inserted into the cell analyzer. The analayzer also deposits a known amount of diluent/stain in the mixing bowl and mixes it with the blood. The analyzer takes a measured amount of the mixture and dispenses in a sample cup on the cartridge in fluid communication with an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping as it is transferred into the imaging chamber by the analyzer. Images of all of the cellular components within the imaging chamber are counted and analyzed to obtain a complete blood count.

This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting and analyzing blood cells in biological samples. A small measured quantity of a biological sample, such as whole blood, is placed in a mixing bowl on the disposable test cartridge after being inserted into the cell analyzer. The analayzer also deposits a known amount of diluent/stain in the mixing bowl and mixes it with the blood. The analyzer takes a measured amount of the mixture and dispenses in a sample cup on the cartridge in fluid communication with an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping as it is transferred into the imaging chamber by the analyzer. Images of all of the cellular components within the imaging chamber are counted and analyzed to obtain a complete blood count.

The present invention provides a characterization system (100) for performing optical characterization of a liquid sample in a process plant, comprising a sample section (103) for holding the liquid sample, an inlet comprising an inlet valve (121) adapted to control a flow of the liquid sample into the sample section (103), an outlet comprising an outlet valve (122) adapted to control a flow of the liquid sample out of the sample section (103), a pressurizer (130) adapted to pressurize the sample section (103), an agitator (140, 800) adapted to agitate at least a part of the liquid sample inside the sample section (103) when the sample section (103) is pressurized by the pressurizer (130), a measuring device (150) adapted to perform optical characterization of the liquid sample inside the sample section (103) while the liquid sample is pressurized and agitated during or after agitation by the agitator, wherein the inlet valve and the outlet valve are connected to a line pipe (202) and the characterization system is adapted to receive the liquid sample from the line pipe through the inlet valve, characterize the liquid sample in the sample section (103), and optionally return at least a part of the liquid sample to the line pipe through the outlet valve.