The invention is concerned with methods for producing a useful and highly uniform optical component which is useful in the construction of an optical sensor. Also discussed are the optical component itself, an optical sensor comprising the optical component, a process for producing the optical sensor and a process for detecting and/or quantifying the amount of an analyte in a sample using the optical sensor.

Novel chemical sensors that improve detection and quantification of CO.sub.2 are critical to ensuring safe and cost-effective monitoring of carbon storage sites. Fiber optic (FO) based chemical sensor systems are promising field-deployable systems for real-time monitoring of CO.sub.2 in geological formations for long-range distributed sensing. In this work, a mixed-matrix composite integrated FO sensor system was developed that reliably operates as a detector for gas-phase and dissolved CO.sub.2. A mixed-matrix composite sensor coating on the FO sensor comprising plasmonic nanocrystals and zeolite embedded in a polymer matrix. The mixed-matrix composite FO sensor showed excellent reversibility/stability in a high humidity environment and sensitivity to gas-phase CO.sub.2 over a large concentration range. The sensor exhibited the ability to sense CO.sub.2 in the presence of other geologically relevant gases, which is of importance for applications in geological formations. A prototype FO sensor configuration which possesses a robust sensing capability for monitoring dissolved CO.sub.2 in natural water was demonstrated. Reproducibility was confirmed over many cycles, both in a laboratory setting and in the field.

Systems, methods, and modules for detecting a biomarker in a sample are described. A system for detecting presence or absence of a biomarker in a sample includes: a light source for producing electromagnetic radiation for interrogating the sample; a biosensor module including: a waveguide for guiding the electromagnetic radiation, the waveguide exposed to the sample; and a recognition element affixed to the waveguide and configured to bind to the biomarker; a detector for receiving the electromagnetic radiation from the waveguide and detecting a signal corresponding to an interaction of the electromagnetic radiation with the biomarker bound to the recognition element, in accordance with at least one detection modality; and a computing device for analyzing data related to the signal in order to detect presence or absence of the biomarker in the sample.

Some embodiments of the present disclosure provide an optical sensor. The optical sensor includes a semiconductive substrate; a light sensing region on the semiconductive substrate; a waveguide region configured to guide light from a wave insert portion through a waveguide portion and to a sample holding portion; and an interconnect region below the waveguide region, and the interconnect region being disposed above the light sensing region. The waveguide portion includes a first dielectric layer comprising a first refractive index and at least one second dielectric layer comprising a second refractive index, wherein the second refractive index is smaller than the first refractive index.

Disclosed herein are embodiments of sensor devices comprising a sensing component able to determine the presence of, detect, and/or quantify detectable species in a variety of environments and applications. The sensing components disclosed herein can comprise MOF materials, plasmonic nanomaterials, redox-active molecules, a metal, or any combinations thereof. In some exemplary embodiments, optical properties of the plasmonic nanomaterials and/or the redox-active molecules combined with MOF materials can be monitored directly to detect analyte species through their impact on external conditions surrounding the material or as a result of charge transfer to and from the plasmonic nanomaterial and/or the redox-active molecule as a result of interactions with the MOF material.

An apparatus for determining the presence or concentration of target molecules comprises: a radiation source; a surface; a waveguide; a detector; and a spectral filter. The radiation source is operable to produce electromagnetic radiation. The surface defines a two dimensional array of receptor sites. The waveguide is arranged to receive the electromagnetic radiation produced by the radiation source, divide the electromagnetic radiation and direct a portion of the electromagnetic radiation to each one of a two dimensional array of receptor sites. The detector comprises a two dimensional array of sensing elements, each sensing element arranged to receive electromagnetic radiation from a different one of the two dimensional array of receptor sites. The spectral filter is provided between the surface and the detector.

This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.

Chalcogenide waveguides with high width-to-height aspect ratios and a smooth exposed surfaces can serve as mid-infrared evanescent-absorption-based sensors for detecting and identifying volatile organic compounds and/or determining their concentration, optionally in real-time. The waveguide sensors may be manufactured using a modified sputtering process in which the sputtering target and waveguide substrate are titled and/or laterally offset relative to each other and the substrate is continuously rotated.

A cartridge for use with an instrument to perform measurement of a fluid, including a digital microfluidics substrate comprising a plurality of electrowetting electrodes operative to perform droplet operations on a liquid droplet in a droplet operations gap; a top plate separated from the digital microfluidics substrate to form a droplet operations gap and comprising openings for injecting liquids into the droplet operations gap; a fiber assembly comprising a fiber optic probe projecting into the droplet operations gap and having a sensing end situated in proximity with one or more of the electrowetting electrodes.

Building blocks are provided for on-chip chemical sensors and other highly-compact photonic integrated circuits combining interband or quantum cascade lasers and detectors with passive waveguides and other components integrated on a III-V or silicon. A MWIR or LWIR laser source is evanescently coupled into a passive extended or resonant-cavity waveguide that provides evanescent coupling to a sample gas (or liquid) for spectroscopic chemical sensing. In the case of an ICL, the uppermost layer of this passive waveguide has a relatively high index of refraction that enables it to form the core of the waveguide, while the ambient air, consisting of the sample gas, functions as the top cladding layer. A fraction of the propagating light beam is absorbed by the sample gas if it contains a chemical species having a fingerprint absorption feature within the spectral linewidth of the laser emission.