An optical detection apparatus includes a substrate, a light source, a light sensor, a light-guiding structure. The substrate has a top surface. The light source on the top surface is configured to generate detection light toward an object over the light source. The light sensor is located on the top surface. The light-guiding structure is above the top surface and at least partially above the light source. A central axis of the light-guiding structure is vertical to the top surface, and the light source and the light sensor are at opposite sides relative to the central axis. The light-guiding structure is configured to deflect the detection light from one of the opposite sides at which the light source is located to another one of the opposite side at which the light sensor is located, such that the detection light reflected by the object moves toward the light sensor.

This invention provides devices for use in various analytical applications including single-molecule analytical reactions. Methods for detecting analytes optically by propagating optical energy by waveguides within a substrate are provided. Analytical devices are provided which have both shallow and deep waveguides in which illumination light is transported through the deep waveguides and coupled into the shallow waveguides. The shallow waveguides provide evanescent field illumination to analytes, such as single-molecule analytes, within nanometer scale wells. Integrated devices including integrated detectors such as CMOS detectors are included.

Disclosed is a glucose measuring apparatus including a pressure measurer having an elastic part or a pressure sensor, that measures a pressure applied to an object, a film that comprises a first optical waveguide configured to be close to the object, a near infrared ray (NIR) irradiator that irradiates an NIR to the first optical waveguide if the measured pressure is greater than or equal to a preset value, an NIR receiver that receives an attenuated total reflection NIR (ATR-NIR) from the first optical waveguide, and an analyzer that measures a blood glucose level based on the ATR-NIR, wherein the film is an independent module that can be combined with and separated from the glucose measuring apparatus.

A photoacoustic apparatus, comprising: at least one optical amplifier, configured to produce light; at least one photonic integrated circuit, configured as a tunable light filter; light guiding means, wherein the at least one optical amplifier, at least one photonic integrated circuit and light guiding means are configured as an optical cavity to produce laser light having an optical path within the optical cavity; and at least one acoustic sensor configured to detect sound produced by analyte introduced into the optical path of the laser light.

Chalcogenide waveguides with high width-to-height aspect ratios and a smooth exposed surfaces can serve as mid-infrared evanescent-absorption-based sensors for detecting and identifying volatile organic compounds and/or determining their concentration, optionally in real-time. The waveguide sensors may be manufactured using a modified sputtering process in which the sputtering target and waveguide substrate are titled and/or laterally offset relative to each other and the substrate is continuously rotated.

A photoacoustic remote sensing system for imaging a subsurface structure in a sample, comprising exactly one laser source configured to generate a pulsed or intensity-modulated excitation beam configured to generate ultrasonic pressure signals in the sample at an excitation location, and an interrogation beam incident on the sample at the excitation location, a portion of the interrogation beam returning from the sample that is indicative of the generated ultrasonic pressure signals, an optical system configured to focus the excitation beam and the interrogation beam below a surface of the sample, a detector configured to detect the returning portion of the interrogation beam, and a processor configured to calculate an image of the sample based on a detected intensity modulation of the returning portion of the interrogation beam from below the surface of the sample.

An integrated semiconductor device for manipulating and processing bio-entity samples and methods are described. The device includes a lower substrate, at least one optical signal conduit disposed on the lower substrate, at least one cap bonding pad disposed on the lower substrate, a cap configured to form a capped area, and disposed on the at least one cap bonding pad, a fluidic channel, wherein a first side of the fluidic channel is formed on the lower substrate and a second side of the fluidic channel is formed on the cap, a photosensor array coupled to sensor control circuitry, and logic circuitry coupled to the fluidic control circuitry, and the sensor control circuitry.

A method of optical analysis comprises receiving light at an optical spectrometer module from a light source, distributing the received light into two or more light beams with a light distribution component of the optical spectrometer module, concurrently exposing each of a reference and one or more test samples to one of the two or more light beams, and concurrently measuring a property of the light associated with each of the reference sample and one or more test samples with a corresponding detector.

A cartridge for use with an instrument to perform measurement of a fluid, including a digital microfluidics substrate comprising a plurality of electrowetting electrodes operative to perform droplet operations on a liquid droplet in a droplet operations gap; a top plate separated from the digital microfluidics substrate to form a droplet operations gap and comprising openings for injecting liquids into the droplet operations gap; a fiber assembly comprising a fiber optic probe projecting into the droplet operations gap and having a sensing end situated in proximity with one or more of the electrowetting electrodes.

Disclosed is an optical flow cell (100, 300) and a method (400) for bioprocessing applications. The optical flow cell (100, 300) comprises a fluid inlet (102, 302), a fluid outlet (104, 304), and a fluid flow channel (106, 306) provided between said fluid inlet (102, 302) and said fluid outlet (104, 304). The optical flow cell (100, 300) also comprises an output optical waveguide (108, 308) configured to emit light into said fluid flow channel (106, 306), and a collector optical waveguide (110, 310) configured to collect light from said fluid flow channel (106, 306). An optical pathlength adjuster (120, 320) for varying the optical pathlength (130, 330) between said output optical waveguide (108, 308) and said collector optical waveguide (110, 310) is also provided.