A method of verifying the efficacy of a disinfection process is provided. The method includes flowing a liquid disinfectant into an indicator device that has a plurality of indicator microorganisms disposed therein, the indicator microorganisms comprising or capable of producing a detectable enzyme activity; contacting the indicator microorganisms with the liquid disinfectant for a predetermined minimum first period of time at a first temperature; after contacting the indicator microorganisms with the liquid disinfectant for the first period of time at the first temperature, contacting the indicator microorganisms for a second period of time with a detection medium comprising a detection reagent; and during or after the second period of time, detecting a quantity of the detectable enzyme activity that was not inactivated by the contact with the disinfectant. The indicator device is also provided.

This invention relates to methods and compositions for detecting, quantifying, or identifying mycotoxins. More particularly, the invention relates to methods and compositions for detecting, quantifying, or identifying a gliotoxin, or a derivative thereof, a mycotoxin of a Penicillium species, or a mycotoxin of a Chaetomium species, in the tissues or body fluid samples of patients.

An immunoadsorbent for purifying fumonisin B.sub.1, aflatoxin B.sub.1, ochratoxin A, zearalenone and sterigmatocystin; a complex affinity column and its preparation method; and a method for detecting the mycotoxins using the same are provided. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B.sub.1 monoclonal antibody, an anti-aflatoxin B.sub.1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B.sub.1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11. The complex affinity column can be used for purification and detection of a fumonisin B.sub.1 sample, an aflatoxin B.sub.1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.

The invention relates to an immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B.sub.1 and aflatoxin B.sub.1, and a complex affinity column. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B.sub.1 monoclonal antibody, an anti-aflatoxin B.sub.1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B.sub.1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11, and the hybridoma cell strain Fm7A11 has been preserved at China Center for Type Culture Collection, Wuhan University, Wuhan, China on Mar. 29, 2016 with the preservation number of CCTCC No. C201636. The complex affinity column can be used for purification and detection of a fumonisin B.sub.1 sample, an aflatoxin B.sub.1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.

This invention relates to methods and compositions for detecting, quantifying, or identifying mycotoxins. More particularly, the invention relates to methods and compositions for detecting, quantifying, or identifying a gliotoxin, or a derivative thereof, a mycotoxin of a Penicillium species, or a mycotoxin of a Chaetomium species, in the tissues or body fluid samples of patients.

An aptamer for specifically detecting patulin and a method for detecting patulin using the same. The aptamer for specifically detecting patulin is a single-stranded DNA aptamer serving as a bioreceptor capable of effectively detecting patulin. Since such an aptamer for specifically detecting patulin is capable of specifically binding to patulin which is a mycotoxin having a simple chemical structure and a low molecular weight, it can be effectively employed as a bioreceptor in various bioassays for specifically detecting patulin. The aptamer for specifically detecting patulin can achieve more effective detection of patulin in apples or apple juice.