A time-resolved fluorescence immunochromatographic test paper card for testing clothianidin. A binding pad includes a detection microsphere and a quality control microsphere thereon, wherein the detection microsphere is a fluorescent microsphere coated with a clothianidin monoclonal antibody on the surface thereof, the quality control microsphere is a fluorescent microsphere coated with a rabbit anti-tag protein on the surface thereof. An NC film is provided with a detection line and a control line. The lengths of the detection line and the control line are the same as the width of the NC film. A first clamping part is arranged at one end of the sample pad. A second clamping part and a third clamping part are respectively arranged at two ends of the binding pad. A fourth clamping part is arranged at one end of the NC film, the other end of the NC film is joined with an absorption pad.

A variable region sequence of a specific antibody against clothianidin is provided. A gene encoding a heavy chain variable region of the antibody has an amino acid sequence shown in SEQ ID NO: 2. An intact recombinant antibody against clothianidin is provided. The intact recombinant antibody includes a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein a gene encoding the heavy chain variable region has an amino acid sequence shown in SEQ ID NO: 2. An immunostrip containing the antibody for rapid detection of clothianidin residue is also provided. The sequence genes obtained are linked to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, respectively, and an intact recombinant antibody is expressed and obtained by using mammalian cells with a double-plasmid system.

The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.

Described herein are murine monoclonal antibodies and methods useful for determining and quantitating the presence of Cry1Ca delta endotoxin. The claimed antibodies specifically bind the core toxin region making them suitable for detecting the native full length Cry1Ca toxin as well as the amino core toxin and N-terminal 29 residue truncated forms.

Disclosed are novel bioactive peptides derived as antagonists to a fire ant receptor for a pheromone biosynthesis-activating neuropeptide/pyrokinin (PBAN/pyrokinin) gene derived neuropeptide ligand. Also disclosed are methods of controlling fire ants with the bioactive peptides disclosed herein. Methodological approaches to screening peptide libraries for the presence of PBAN/pyrokinin ligands are also provided herein.

This disclosure provides a method and a kit for pesticide detection. By expressing acetylcholinesterases on cell surface, rapid pesticide screening, identification and quantification of pesticides or insecticides may be achieved.

A hybridoma cell strain secreting anti-dinitolmide monoclonal antibodies deposited at China General Microbiological Culture Collection Center (CGMCC) at No. 1 West Beichen Road, Chaoyang District, Beijing 100101, China on Nov. 28, 2019, with deposit number of CGMCC No. 19165. It is classified as a monoclonal cell strain. The monoclonal antibody secreted by the hybridoma cell strain DAS3H10 has a good affinity and high sensitivity to dinitolmide. Because of IC.sub.50 to dinitolmide up to 9.01 ng/mL, the monoclonal antibody could be used to prepare dinitolmide immunoassay kit and colloidal gold test strip and provides a powerful detection method and means for the detection of dinitolmide in animal-derived foods.

This invention provides polypeptides that were identified as interacting with Vip3 toxin. This invention also provides a method of identifying agents that bind to Vip3 interacting polypeptides, which agents may act as insecticidal agent, cytotoxic agents and/or modulate the activity of Vip3 interacting polypeptides.

A time-resolved fluorescence immunochromatographic test paper card for testing clothianidin. A binding pad includes a detection microsphere and a quality control microsphere thereon, wherein the detection microsphere is a fluorescent microsphere coated with a clothianidin monoclonal antibody on the surface thereof, the quality control microsphere is a fluorescent microsphere coated with a rabbit anti-tag protein on the surface thereof. An NC film is provided with a detection line and a control line. The lengths of the detection line and the control line are the same as the width of the NC film. A first clamping part is arranged at one end of the sample pad. A second clamping part and a third clamping part are respectively arranged at two ends of the binding pad. A fourth clamping part is arranged at one end of the NC film, the other end of the NC film is joined with an absorption pad.

A time-resolved fluorescence immunochromatography kit for the simultaneous detection of a mixed pollutant of aflatoxin and carbaryl, a preparation method therefor, and an application thereof. The kit comprises a time-resolved fluorescence immunochromatography test strip and a sample reaction vial containing a europium-labeled anti-aflatoxin monoclonal antibody and a europium-labeled anti-carbaryl monoclonal antibody lyophilized product, detection lines of the time-resolved fluorescence immunochromatography test strip being coated with an aflatoxin-bovine serum albumin conjugate and a carbaryl-ovalbumin conjugate respectively, and the anti-carbaryl monoclonal antibody is produced by secretion of a hybridoma cell line Jnw1D2 with a preservation number of CCTCC NO. 0201654. The kit can be used for the simultaneous detection of aflatoxin and carbaryl content in a sample, and features simple and quick operations and a high sensitivity.