Methods, apparatus, systems and articles of manufacture to make and/or process diagnostic tests are disclosed. An example test device, such as a lateral flow immunoassay device, includes a conjugate pad including conjugates for a target analyte; and a test grid including a plurality of zones in a two-dimensional grid, the test grid including: a first test zone in the plurality of zones including at least one of first immobilized antibodies or first immobilized antigens to attach to the target analyte labeled with a first conjugate of the conjugates; and a second test zone in the plurality of zones including at least one of second immobilized antibodies or second immobilized antigens to attach to the target analyte labeled with a second conjugate of the conjugates.

The present application discloses methods of making anti-hepsin antibodies, anti-hepsin antibodies, methods of screening the activity of anti-hepsin antibodies, pharmaceutical compositions of anti-hepsin antibodies, kits containing anti-hepsin antibodies, and methods of using anti-hepsin antibodies to diagnose a cancer.

Provided herein are assays that provide digital measurement methods to detect proteins and other biomolecules, e.g., at low- to mid-attomolar concentrations.

The present disclosure provides methods and kits for detecting Group A Streptococcus in biological samples. More particularly, the present disclosure provides methods for enhancing the specificity and sensitivity of Group A Streptococcus immunoassays by including N-propionyl-D-glucosamine, 2-N-butanoyl-D-glucosamide, Bis-(2-(D-2-deoxy-glucosaminyl))-PEG3-amide, m-PEG4-glucosamine, m-PEG6-glucosamine, or m-PEG10-glucosamine. The methods and kits disclosed herein are thus useful for reliable and early diagnosis of streptococcal infections in a subject.

The present invention relates to methods for detecting a target molecule in a liquid sample. The methods described herein comprise applying at least a portion of the liquid sample on a solid substrate that comprises a non-fouling polymer layer, decreasing the atmospheric pressure surrounding the solid substrate containing the portion of the liquid sample for a time sufficient for a majority of the liquid to evaporate from the portion of the liquid sample applied to the solid substrate, contacting the liquid sample with one or more binding agents that binds to the target molecule after the majority of the liquid has evaporated from the liquid sample, and detecting the presence of the one or more binding agents on the solid substrate, wherein the presence of the one or more binding agents indicates the presence of the target molecule in the liquid sample.

The present invention relates to a cartridge for sandwich ELISA pre-loaded with antigen customized detection reagent to more efficiently perform the sandwich ELISA and a sandwich ELISA device using the cartridge. A cartridge for sandwich ELISA according to the present invention is arranged in rows and columns, and includes a body in which wells accommodating reagents are formed, a pin-assy including pins that have a capture antibody coated on one ends thereof, and a provider that moves the pin-assy to provide the well with the pin to allow the reagents and the capture antibody to be into contact and react with each other.

An object of the present invention is to provide a method of assisting diagnosis of inflammatory bowel disease, which can specifically determine inflammatory bowel disease.

The present invention relates to “a method of assisting diagnosis of inflammatory bowel disease, the method including subjecting a subject-derived specimen to a reduction treatment and subsequently measuring a human prohaptoglobin amount in the specimen by using an antibody 1 which is an antibody that specifically binds to an amino acid sequence set forth in SEQ ID NO: 1, and determining that a subject has inflammatory bowel disease by using the human prohaptoglobin amount as an indicator, and relates to an examination kit for assisting diagnosis of inflammatory bowel disease, including the antibody 1 a reducing agent”.

Provided is a method for the quantitative and/or qualitative determination of bioindicators, including the following steps: a) immobilizing capture molecules for the bioindicators on a substrate; b) bringing the bioindicators of a sample into contact with the capture molecules; c) immobilizing the bioindicators on the substrate by binding to capture molecules; d) bringing the bioindicators into contact with probes containing at least one detection molecule, and e) removing non-specifically bound molecules and particles; and f) binding the probes to the bioindicators, wherein the probes are capable of emitting a specific detection signal and steps b) and d) can take place simultaneously or d) before b), and wherein probes and capture molecules are used which have affine molecules or molecule parts that bind to at least one specific binding site of the bioindicators and these affine molecules or molecule parts of the probes and capture molecules do not overlap one another.

Disclosed herein is a method of detecting the presence of a target analyte in a sample. Disclosed herein are also a system, an article, and a kit for detecting the presence of a target analyte in a sample. Disclosed herein is also the use of the system, or the article, or the kit for biomolecule, bioorganelle, bioparticle, cell and microorganism detection.

An optical probe for a bio-sensor selectively conjugated to a target analyte and configured to retro-reflect incident light thereto is disclosed. The optical probe for the bio-sensor includes: a transparent core particle; a total-reflection inducing layer covering a portion of a surface of the core particle, the inducing layer is made of a material having a refractive index lower than a refractive index of the core; a modifying layer formed on the total-reflection inducing layer; and an analyte-sensing substance bound to the modifying layer, the sensing substance is selectively conjugated to the target analyte. This optical probe may serve as an excellent optical probe for both a non-spectral light source and a spectral light source.