Provided is, for example, a method for analyzing steroid hormones contained in a body hair sample from an animal, wherein body hair collected from an animal is used as the body hair sample, and the method includes: an extraction step of extracting a plurality of types of steroid hormones from the body hair sample by using a 45 vol % to 55 vol % aqueous acetonitrile solution containing 0.1 M trifluoroacetic acid; and a measurement step of measuring the amounts of the plurality of types of steroid hormones extracted.

The invention described herein relates to methods of monitoring genotoxic stress in a test subject, specifically by detecting the expression level of microvesicle-associated H2AX from a biological sample.

The invention described herein relates to methods of monitoring genotoxic stress in a test subject, specifically by detecting the expression level of microvesicle-associated H2AX from a biological sample.

The invention relates to methods of protecting against chronic stress in a subject and treating stress sensitive disorders in a subject by antagonizing ghrelin or ghrelin receptor.

Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as Cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N-ethylcarbodiimide and 10 mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73 pM and less than 10% relative standard deviation.

There is described a method for assaying for loss of viability of a photosynthetic organism (preferably a microorganism) in an aqueous liquid. In a preferred embodiment, the method comprises the step of using fluorescence to correlate the photorepair index for the organism in the aqueous liquid to survivorship of the organism (preferably a microorganism) after it is exposed to ultraviolet radiation, thereby assessing viability.

The present disclosure describes pan-SUMO trapping proteins and fusion proteins comprising the pan-SUMO trapping proteins that are stable and bind SUMO-modified proteins with high avidity. The proteins described herein can be used to detect the localization of SUMO-modified proteins cells. The proteins described herein can be used to identify biomarkers for diseases associated with oxidative stress. They can also be used to diagnose and monitor diseases associated with genotoxic and/or proteotoxic stress conditions.

The present invention relates to the field of biomarkers. More specifically, the present invention relates to the use of biomarkers to predict post-traumatic stress disorder (PTSD). In one embodiment, a method for predicting PTSD in a subject comprises the steps of (a) measuring the DNA methylation level of a CpG dinucleotide in the 3′ untranslated region of SKA2; (b) identifying the genotype at a SNP within the 3′ UTR of SKA2, and (c) predicting PTSD in the subject using a prediction algorithm.

Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N-ethylcarbodiimide and 10 mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73 pM and less than 10% relative standard deviation.

The present invention relates to a method for mimicking the effects of ionizing radiations on cells, wherein cells are contacted with bleomycin.