A pump unit includes a pump and a metal ion trap. The pump includes a metal member that comes into contact with a mobile phase. The pump pumps a mobile phase through a flow path. The metal ion trap includes a filter element. The filter element includes a metal ion retention structure for retaining metal ions by interacting with the metal ions included in a mobile phase. The filter element is provided in the flow path for a mobile phase pumped by the pump. The pump unit may be provided in a chromatograph.

A pump unit includes a pump and a metal ion trap. The pump includes a metal member that comes into contact with a mobile phase. The pump pumps a mobile phase through a flow path. The metal ion trap includes a filter element. The filter element includes a metal ion retention structure for retaining metal ions by interacting with the metal ions included in a mobile phase. The filter element is provided in the flow path for a mobile phase pumped by the pump. The pump unit may be provided in a chromatograph.

In a pretreatment method, in a first step, a sample is dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol to prepare a first solution. In a second step, an organic base that has a lower boiling point than that of HFIP is added to the first solution to prepare a second solution. In a third step, the second solution is heated to obtain a substance in which an anhydrous oxide structure in the sample has been decomposed. In a fourth step, chloroform is added to the second solution to prepare a third solution.

In a pretreatment method, in a first step, a sample is dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol to prepare a first solution. In a second step, an organic base that has a lower boiling point than that of HFIP is added to the first solution to prepare a second solution. In a third step, the second solution is heated to obtain a substance in which an anhydrous oxide structure in the sample has been decomposed. In a fourth step, chloroform is added to the second solution to prepare a third solution.

In a pretreatment method, in first step, a sample is dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol to prepare a first solution. In second step, an organic base is added to the first solution to prepare a second solution. In third step, the second solution is heated to obtain a substance in which an anhydrous oxide structure in the sample has been decomposed. In a fourth step, an organic solvent that has a higher boiling point than that of 1,1,1,3,3,3-hexafluoro-2-propanol and is compatible (miscible) with 1,1,1,3,3,3-hexafluoro-2-propanol is added to the second solution to prepare a third solution.

In a pretreatment method, in first step, a sample is dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol to prepare a first solution. In second step, an organic base is added to the first solution to prepare a second solution. In third step, the second solution is heated to obtain a substance in which an anhydrous oxide structure in the sample has been decomposed. In a fourth step, an organic solvent that has a higher boiling point than that of 1,1,1,3,3,3-hexafluoro-2-propanol and is compatible (miscible) with 1,1,1,3,3,3-hexafluoro-2-propanol is added to the second solution to prepare a third solution.

The present invention relates to a method of measuring at least one product quality attributes, including critical quality attributes (e.g., oxidation, C-terminal lysine, aggregation, N-terminal glutamine cyclization.) by an online liquid chromatography system, wherein the liquid chromatography system is running a hydrophobic interaction chromatography (HIC) method.

The present invention relates to a method of measuring at least one product quality attributes, including critical quality attributes (e.g., oxidation, C-terminal lysine, aggregation, N-terminal glutamine cyclization.) by an online liquid chromatography system, wherein the liquid chromatography system is running a hydrophobic interaction chromatography (HIC) method.

Described is a radial flow column for conveying a liquid product through an adsorber material, more particularly for radial flow chromatography, having a housing and, arranged therein, an inner and an outer screen, each of which surrounds a longitudinal axis of the housing and between which a radial intermediate space for receiving the adsorber material is formed. The end-face end regions of the inner and outer screen are sealed with respect to the housing in order to convey the product radially through the screens and the adsorber material. Because the radial flow column comprises ring seals for radially sealing the end-face end regions and the ring seals with corresponding sealing faces permit an axial play of the screens in the housing, production tolerances of the screens with respect to one another and in relation to the housing, and longitudinal fluctuations of the screens, can be compensated in a material-preserving manner.

Described is a radial flow column for conveying a liquid product through an adsorber material, more particularly for radial flow chromatography, having a housing and, arranged therein, an inner and an outer screen, each of which surrounds a longitudinal axis of the housing and between which a radial intermediate space for receiving the adsorber material is formed. The end-face end regions of the inner and outer screen are sealed with respect to the housing in order to convey the product radially through the screens and the adsorber material. Because the radial flow column comprises ring seals for radially sealing the end-face end regions and the ring seals with corresponding sealing faces permit an axial play of the screens in the housing, production tolerances of the screens with respect to one another and in relation to the housing, and longitudinal fluctuations of the screens, can be compensated in a material-preserving manner.