A gas phase component analysis device and a gas phase component analysis method that can prevent degradation of the device due to an unnecessary component and can obtain excellent detection sensitivity are provided.

A gas phase component analysis device (1) includes a heating unit (2) configured to heat a specimen to generate a gas phase component composite, a first column (31) into which the gas phase component composite is introduced, a second column (32) that is a separation column connected with the first column (31) through a connection unit (33), an isothermal oven (3) housing the first column (31), the second column (32), and the connection unit (33), a detection unit (4) configured to detect a gas phase component having passed through the second column (32), and a suction unit (5) connected with the connection unit (33).

A resin tube connection device includes a resin tube, a resin expanding portion and a metal sleeve. The resin expanding portion has a sealing surface being able to come into contact with a contact surface. Further, the resin expanding portion has a second flow path that communicates with a first flow path of the resin tube and opens in the sealing surface. The sealing surface projects farther than an end surface of the metal sleeve in an axial direction. The maximum length of a contact region where the resin expanding portion and the metal sleeve come into contact with each other in the axial direction in an outer surface is larger than the thickness of a resin layer in a radial direction.

Provided is a method for simultaneously detecting Vitamin K1 and Vitamin K2 in traces of blood. The method includes: constructing a two-dimensional liquid chromatography-tandem mass spectrometer, establishing an analytical method, and detecting at least three mixed standard solutions using the constructed two-dimensional liquid chromatography-tandem mass spectrometer to obtain a first detection result; fitting standard curve equations respectively corresponding to Vitamin K1 and Vitamin K2; and mixing and centrifuging a blood sample to which an extraction reagent and a certain amount of internal standard substance are added, collecting a supernatant, blowing the supernatant to dry with nitrogen, redissolving the residue, and detecting the dry supernatant using the constructed two-dimensional liquid chromatography-tandem mass spectrometer to obtain a second detection result. In this manner, concentrations of Vitamin K1 and Vitamin K2 in the blood sample are obtained.

A method for monitoring, evaluating and controlling a cyclic chromatographic purification process that involves at least two adsorbers. According to the method, one step is monitoring of the chromatogram, including the measurement of at least one current concentration-proportional signal in the liquid. Another step is conducting an evaluation of the chromatogram, including a comparison of at least one of the current concentration-proportional signals measured in the monitoring step with a threshold value thereof. A further step is controlling the chromatographic purification process by adapting the termination of the currently running phase as a function of the comparison of the evaluation step and initiating the next phase. Finally, according to the method, the sequence of steps is carried out in given order at least twice.

Methods and systems for removing gases and/or pressure formed during the sterilization, e.g., the gamma irradiation, of prepacked chromatography systems (column plus attached tube and valve set) are described. The methods include purging the gas and/or pressure through specially designed tube and valve sets without breaching the sterility of the prepacked sterile chromatography system. The systems include a sterile or aseptic pre-packed chromatography column including a column having an inlet and an outlet, a tubing and valve set attached to the inlet and the outlet, and a pump configured to pump sterile or aseptic liquid from the fluid source along the tubing and valve set, into the column tube inlet and out of the column tube outlet along a first flow path, thereby removing any entrapped gas and/or pressure from the chamber.

Methods and systems for removing gases and/or pressure formed during the sterilization, e.g., the gamma irradiation, of prepacked chromatography systems (column plus attached tube and valve set) are described. The methods include purging the gas and/or pressure through specially designed tube and valve sets without breaching the sterility of the prepacked sterile chromatography system. The systems include a sterile or aseptic pre-packed chromatography column including a column having an inlet and an outlet, a tubing and valve set attached to the inlet and the outlet, and a pump configured to pump sterile or aseptic liquid from the fluid source along the tubing and valve set, into the column tube inlet and out of the column tube outlet along a first flow path, thereby removing any entrapped gas and/or pressure from the chamber.

One aspect of the invention provides a gas chromatography union including: a first port; a second port in fluid communication with the first port; and a first pair of wings defining a first recess. Another aspect of the invention provides a union including: a first port; a second port in fluid communication with the first port; and at least a first protrusion defining a first recess. Another aspect of the invention provides a coupler including: a first port; a second port in fluid communication with the first port; and a first connector positioned on a portion of a surface of the coupler.

A liquid junction assembly for providing a flow connection between two tubular conduits. The assembly includes respective bodies configured to define elongated passages of respective first and second cross sections to receive and locate the respective tubular conduits, a plate with at least one hole therethrough of a third cross section smaller than the first and second cross sections, and a seat for the plate, defined in a face of one or both of the bodies. The bodies and the plate are assembled with the plate in the seat and the elongated passages and the hole aligned along a common axis.

The invention provides compression fittings and methods of assembling compression fittings. In exemplary embodiments, compression fittings are provided that include a fitting body, a ferrule and a tube. For example, the fitting body can be removably coupled to the ferrule when the tube is disposed therethrough.

One aspect of the invention provides a gas chromatography union including: a first port; a second port in fluid communication with the first port; and a first pair of wings defining a first recess. Another aspect of the invention provides a union including: a first port; a second port in fluid communication with the first port; and at least a first protrusion defining a first recess. Another aspect of the invention provides a coupler including: a first port; a second port in fluid communication with the first port; and a first connector positioned on a portion of a surface of the coupler.