A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of vapor deposition coated frit(s) in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having coated frit(s) within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

A biocompatible tube and fitting system that can be used in a liquid chromatography system is described. The tube can have a polymer tip and can be used in conjunction with one or more fitting assembly.

A crescent PLOT column is disclosed, including a capillary column having an inlet, an outlet, a bore, and an inner surface surrounding the bore and extending between the inlet and the outlet. A layer of particles is localized on a radial portion of the inner surface. The layer of the particles includes a radial thickness decreasing from a center of the radial portion to a periphery of the radial portion, forming a crescent shape in a radial frame of reference. A method for preparing the crescent PLOT column is disclosed, including loading the capillary column with a fluid including a carrier and particles such that the fluid is contained within the capillary column. The capillary column and the fluid contained within the capillary column are subjected to a centrifugal force. The carrier is removed, and a layer of the particles is localized on the radial portion of the inner surface.

A thermal desorption tube collection system uses a thermoelectric cooler to collect and concentrate gas samples. In some modes, the operation of the cooler is reversed to flow the concentrated sample directly into a separator such as a gas chromatography system. Components resolved in time by a thermal desorption separator accumulate in a sample cell and are analyzed by electromagnetic radiation-based spectroscopic techniques. Also presented are methods for analyzing biogas samples.

An apparatus and method for reclaiming and filtering helium for reuse in analytical instruments. The method includes inputting the output gas stream from a gas chromatograph split vent and subjecting the input gas stream to a mini-particulate filter. The gas stream is also subjected to a molecular sieve filter and a finishing filter to isolate the helium carrier gas. The finishing filter removes trace contaminants that are not caught in the previous filters. The method utilizes a pump and controller to maintain a constant pressure, preferably between 80 and 100 psi, to avoid back-flow contamination and to ensure movement of the gas stream through the filters. Additionally, the use of a relief valve prevents back pressure from entering into the gas chromatograph. The filtered helium gas may be stored for future use or re-introduced directly to the input carrier gas stream of a gas chromatograph.

Liquid chromatography and gas chromatography components with a conformal protective coating on internal surfaces thereof and methods for producing the same are provided. The conformal protective coating is formed via an atomic layer deposition (ALD) process. The components and methods of the present disclosure find use in providing protective coatings for liquid chromatography and gas chromatography components that are compatible with bioanalytical separations and sensitive trace analyses.

A multi-walled tube that is useful as an analytical column in which chemical mixtures can be separated into their individual components is described. In order to be acceptable as an analytical column, the inner surface of the multi-walled tube must support effective separation, but not react chemically with or contaminate the solvent or the analyte (sample to be separated). Grade 316 stainless steel is typically preferred for this purpose. Moreover, the inner diameter (ID) surface of the multi-walled column is preferably very smooth (less than 10 micro inch Ra) with no interruptions in the surface such as scratches, pits, or asperities. However, since the column is designed to be attached to chromatographic equipment using standard size connection features, the size of standard fittings define the outer diameter (OD) of the column.

A mechanism is provided in a data processing system comprising a processor and a memory, the memory comprising instructions that are executed by the processor to specifically configure the processor to implement a repetitive portion identification and weighting engine. A machine learning model is trained for weighting repetitive portions of patient electronic medical records (EMRs). A repetitive portion identification component applies a plurality of templates to clinical notes of a patient EMR to identify one or more candidate portions that match at least one of the plurality of templates. A content analysis component performs content analysis on the one or more candidate portions to determine whether each given candidate portion is relevant. A weighting component assigns a relative weight to each given candidate portion based on relevance. A cognitive summary graphical user interface (GUI) generation component generates cognitive summary reflecting at least a subset of the one or more candidate portions of the patient EMR. The mechanism outputs the cognitive summary in a GUI to a user.

Disclosed is a chromatography column cylinder (200) formed from a polymer composite comprising an outer layer (220) and inner layer (210) intended to be in direct contact with the intended contents of the cylinder in a volume (202), wherein the outer layer supports the inner layer, for example when there is negative pressure in the volume (202). In one embodiment, one of said layers is molded directly onto a surface of the other layer such that there is no gap between the two layers. Disclosed also is a method for forming a chromatography column cylinder.

A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel.