The disclosure features calibration fluids that include a plurality of beads, such as cellulose, silica, poly(methyl-methacrylate) (PMMA), melamine, cross-linked agarose, polyvinylacetate (PVA), and/or polystyrene beads, where the beads are sized and colored to represent at least one type of blood cell; and a carrier fluid, that can include a polymer or polymerizing matrix, e.g., a water-soluble resin, e.g., an acrylic or polyurethane water-soluble resin or a starch or a cellulose, serum, and/or one or more or sugars. The disclosure also features methods of using the calibration fluids to calibrate automated sample preparation systems, such as automated hematology analyzer systems, e.g., image-based hematology analyzer systems.

The disclosure features calibration fluids that include a plurality of beads, such as cellulose, silica, poly(methyl-methacrylate) (PMMA), melamine, cross-linked agarose, polyvinylacetate (PVA), and/or polystyrene beads, where the beads are sized and colored to represent at least one type of blood cell; and a carrier fluid, that can include a polymer or polymerizing matrix, e.g., a water-soluble resin, e.g., an acrylic or polyurethane water-soluble resin or a starch or a cellulose, serum, and/or one or more or sugars. The disclosure also features methods of using the calibration fluids to calibrate automated sample preparation systems, such as automated hematology analyzer systems, e.g., image-based hematology analyzer systems.

The present invention relates to a transfer platform for a biological sample analyzer, comprising a movable carrier plate and an object stage which is placed on the carrier plate and separable from the carrier plate. The side of the carrier plate facing the object stage is an upper surface, and the reverse side is a lower surface. The side of the object stage facing the carrier plate is a lower surface, and the reverse side is an upper surface. The object stage and the carrier plate are respectively provided with magnetic blocks that magnetically attract each other. When the object stage is placed in a predetermined area on the carrier plate, the suction of the magnetic blocks automatically positions the object stage relative to the carrier plate, thereby achieving the technical effect of blind positioning. The present invention can be applied to test analyzers for specific proteins, cholesterol, heme, routine urine test, dry biochemical test, etc. The present invention has the advantages of convenient operation, time and effort saving, high efficiency and the like. Meanwhile, the present invention is simple in structure and low in cost, and is suitable for wide promotion.

Disclosed is the synthesis procedure for benzazolo[3,2-a]quinolinium chloride salts and the inclusion of chloro-substituent, amino-substituent, and nitro-substituent resulting in several compounds. The compounds are further used as markers due to their fluorescent properties including in hypoxic environments.

This disclosure further describes anti-cancer screening of two BQS, namely, 7-benzyl-3-aminobenzimidazo[3,2-a]quinolinium chloride (ABQ-48: NSC D-763307) and the corresponding 7-benzyl-3-nitrobenzimidazo[3,2-a]quinolinium chloride (NBQ 48: NSC D-763303).

Disclosed is the synthesis procedure for benzazolo[3,2-a]quinolinium chloride salts and the inclusion of chloro-substituent, amino-substituent, and nitro-substituent resulting in several compounds. The compounds are further used as markers due to their fluorescent properties including in hypoxic environments.

This disclosure further describes anti-cancer screening of two BQS, namely, 7-benzyl-3-aminobenzimidazo[3,2-a]quinolinium chloride (ABQ-48: NSC D-763307) and the corresponding 7-benzyl-3-nitrobenzimidazo[3,2-a]quinolinium chloride (NBQ 48: NSC D-763303).

A method of analyzing a glycated protein, including reacting a color developer represented by the following Formula (1) with a biological sample; and measuring a color reaction of the color developer,

##STR00001## wherein, in Formula (1), X is an alkali metal ion. The biological sample may be a serum sample or a plasma sample. The biological sample in a liquid state may be added to the color developer in a dry state.

A method of analyzing a glycated protein, including reacting a color developer represented by the following Formula (1) with a biological sample; and measuring a color reaction of the color developer,

##STR00001## wherein, in Formula (1), X is an alkali metal ion. The biological sample may be a serum sample or a plasma sample. The biological sample in a liquid state may be added to the color developer in a dry state.

A method of determining concentration of an analyte in a body fluid uses a mobile device having a camera and an ambient light detector. The ambient light detector has an ambient light sensor and/or an additional camera. An ambient light check is performed. In the ambient light check, ambient light is used to determine an item of ambient light information. If a validity criterion is determined to be met, the concentration of the analyte is determined by evaluating an image of at least a part of a reagent test region of an optical test strip having a sample of body fluid applied thereto. The image is captured by the camera. A mobile device, a kit, a computer program and a computer-readable storage medium are also disclosed.

Zwitterionic luminescent polymeric nanoparticles, which include at least one luminescent dye and at least one random copolymer, and the method of preparing the zwitterionic luminescent polymeric nanoparticles. Also, the use of these zwitterionic luminescent polymeric nanoparticles in the medical field and in the biological research field for in vitro or in vivo detection or tracking of a target biological molecule.

It is an object of the present invention to provide improved lateral flow test devices that can provide sensitive and accurate quantitative test results, and methods for the manufacture thereof.