The present invention provides metallic nanocluster (NC)—antibody (Ab) conjugate, wherein the NC has an average size lower than 3 nm and the conjugate: (a) comprises one single NC, (b) the antibody maintains the binding ability; and (c) has a catalytic activity selected from peroxidase-like and photocatalytic activity.

The invention also provides a process for the preparation of the conjugate as well as compositions, kits and uses in therapy and diagnostics.

Advantageously, the conjugate of the invention shows improved catalytic activity, providing a remarkable improved immunoassay's sensitivity.

A CuInS.sub.2@ZnS nanomaterial synthesized with thiosalicylic acid and sodium citrate as dual-stabilizers is taken as a chemiluminescent luminophore, and Tris buffer containing both N.sub.2H.sub.4.H.sub.2O and H.sub.2O.sub.2 is taken as the triggering solution; introducing the H.sub.2O.sub.2 into the triggering solution can bring out greatly enhanced CL emission and obviously shortened CL process, enable the CuInS.sub.2@ZnS nanomaterial with strong flash-type and near-infrared CL; the luminophore of CuInS.sub.2@ZnS nanomaterial is synthesized by a one-pot method; compared with acridinium ester (a classical flash-type chemiluminescent substance), the CuInS.sub.2@ZnS nanomaterial is simple in synthesis method, mild in conditions and short in the required time, the synthesized CuInS.sub.2@ZnS nanomaterial is not easy to decompose under light, and the CL waveband is in the near-infrared region.

The present disclosure relates to a conjugated quantum dot nanoparticles, to methods of making such conjugated quantum dot nanoparticles, and to methods of detecting DNA methylation using such conjugated quantum dot nanoparticles.

A method to determine the throughput speed v of a pore, comprising the steps of feeding, by means of a driving force F, a filiform calibration element through the pore, the calibration element having a plurality of markers spaced apart by known distances and configured to produce an interaction event that transmits a signal away from the pore upon interaction with the pore, detecting a plurality of interaction events, and determining a time interval Δt between successive interaction events, and/or a frequency ω of interaction events.

Apparatus and methods for the detection of proteins in biological fluids such as urine using a label-free assay is described. Specific proteins are detected by their binding to highly specific capture reagents such as SOMAmers that are attached to the surface of a substrate. Changes to these capture reagents and their local environment upon protein binding modify the behavior of color centers (e.g., fluorescence, ionization state, spin state, etc.) embedded in the substrate beneath the bound capture reagents. These changes can be read out, for example, optically or electrically, for an individual color center or as an average response of many color centers.

A quantum dot microscope apparatus is provided. A further aspect employs a tilted or tapered end or tip on a microscopic probe. Another aspect of the present apparatus employs a probe including a quantum dot with only one tunneling lead connected to a power source. A manufacturing aspect includes creating a tapered or asymmetrically shaped specimen-facing end of a probe where a quantum dot is located on the end. A further manufacturing aspect includes using focused ion-beam milling to create a tip or end of a quantum dot microscope probe.

The present disclosure provides a method for detection of an analyte in a sample, where the sample is introduced into an analytic chamber along with droplets of an emulsion or gel beads. In another aspect, the present disclosure provides designs for formulations of emulsion drops or gel beads such that they are useful for detection of analytes in a massively parallel manner. Formulations that contain specific combinations of fluorescent particles allow optical determination of the identity of each fluorescent particle. The combinations are based on particle fluorescence emission wavelength, fluorescence excitation wavelength, and particle count.

The invention encompasses a real time, point of care, diagnostic system, including a lateral flow test cassette, a data reader, and an application for processing test results and methods of diagnosis of a disease or virus.

Provided herein are compositions and methods for identifying or quantitating one or more analytes in sample. The composition can comprise an affinity molecule reversibly conjugated to a label moiety via a double-stranded nucleic acid linker or via an adaptor molecule. The affinity molecule and the label moiety can be linked to different strands of the double-stranded nucleic acid linker. Compositions can be used in any biological assays for detection, identification and/or quantification of target molecules or analytes, including multiplex staining for molecular profiling of individual cells or cellular populations. For example, the compositions can be adapted for use in immunofluorescence, fluorescence in situ hybridization, immunohistochemistry, western blot, and the like.

Provided are a novel immuno-optomagnetic point-of-care (PoC) assay and in particular, a method for detecting an analyte using magnetic nanoparticles and quantum dots (QD) having antibodies which are interfaced with the fabricated PoC biochip platform for quantitative analysis, and an immuno-optomagnetic detection method. The method also relates to methods of making such a plurality of conjugated magnetic quantum dot nanoparticles, methods of detecting analytes using such a plurality of conjugated quantum dot nanoparticles.