An optical device, such as a microscope, is disclosed that can be assembled from flat materials. The optical device can be assembled via a series of folds of a flat material. The optical microscope can include a stage for supporting a sample, an optic stage, and a light source. The optic stage can include one or more lenses. The optical microscope can be capable of obtaining simultaneous images from different forms of microscopy. The optical microscope may have bright field and filter field viewing capabilities wherein a user shifts from bright field to filter field by lateral movement of the stage containing a lens and a light source that cooperate to provide either the bright field or the filter field.

Embodiments described herein relate to a large area lens-free imaging device. One example is a lens-free device for imaging one or more objects. The lens-free device includes a light source positioned for illuminating at least one object. The lens-free device also includes a detector positioned for recording interference patterns of the illuminated at least one object. The light source includes a plurality of light emitters that are positioned and configured to create a controlled light wavefront for performing lens-free imaging.

The present subject matter described an optical microscopy device (2) for a portable imaging system, such as a smartphone. The optical microscopy device (2) comprises an optical lens assembly with ten to sixteen lens elements. The optical lens assembly has an optical magnification in a range of about 1X to about 3X, an airy radius in a range of about 3.2 micron to about 15 micron, a depth of field in a range of about 28 micron to about 133 micron, a numerical aperture in a range of about 0.025 to about 0.176, a half field of view in a range of about 10 degrees to about 39 degrees, and a length in a range of about 6.8 millimeter (mm) to about 18 mm.

The present subject matter described an optical microscopy device (3) for a portable imaging system, such as a smartphone. The optical microscopy device (3) comprises an optical lens assembly with eight to fifteen lens elements. The optical lens assembly has an optical magnification in a range of about 1× to about 7.8×, an airy radius in a range of about 3 micron to about 23.25 micron, a depth of field in a range of about 20 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.115, a half field of view in a range of about 12 degrees to about 30 degrees, and a length in a range of about 6.5 millimeter (mm) to about 57 mm.

Among other things, an imaging device has a photosensitive array of pixels, and a surface associated with the array is configured to receive a specimen with at least a part of the specimen at a distance from the surface equivalent to less than about half of an average width of the pixels.

A method for obtaining a Fourier ptychography image using a smartphone comprises the steps of: (a) sequentially providing illumination of different angles to the sample by sequentially displaying, according to a first pattern composed of point light sources at different positions, the point light sources of the first pattern on a display of the smartphone; (b) obtaining an image for each illumination angle of the sample using a camera of the smartphone whenever illumination of different angles is provided by the point light sources of the first pattern; and (c) restoring a first Fourier ptychography image using a plurality of images for each illumination angle obtained using the camera of the smartphone.

A method for a system and method for morphometric detection of malignancy associated change (MAC) is disclosed including the acts of obtaining a sample; imaging cells to produce 3D cell images for each cell; measuring a plurality of different structural biosignatures for each cell from its 3D cell image to produce feature data; analyzing the feature data by first using cancer case status as ground truth to supervise development of a classifier to test the degree to which the features discriminate between cells from normal or cancer patients; using the analyzed feature data to develop classifiers including, a first classifier to discriminate normal squamous cells from normal and cancer patients, a second classifier to discriminate normal macrophages from normal and cancer patients, and a third classifier to discriminate normal bronchial columnar cells from normal and cancer patients.

A mobile phone-based dark field microscope (MDFM) apparatus suitable for quantifying nanoparticle signals is provided. The MDFM apparatus includes an electrically operated light source, a dark-field condenser, a slide housing configured to receive an analytical slide, and an adapter housing configured to receive an objective lens and receive a portable electronic communication device. The slide housing positions the analytical slide between the objective lens and the dark-field condenser. The adapter housing registers the objective lens with a camera lens of the portable electronic communication device. A method for performing a biological quantitative study using the dark-field microscope apparatus is further provided.

A microscopy imaging system and methods for improving an assaying of a sample is disclosed.

Provided is a solid-state illumination system for use in a microscopy system utilizing a light sensor of a mobile phone camera module. The system includes a bright-field illumination source with an array of light-emitting diodes (LEDs). The array of LEDs is configured to produce transmission light within a range of view of the light sensor of the mobile phone camera module. The system also includes a dark-field illumination source including a ring of LEDs. The ring of LEDs is configured to produce light outside of the range of collection of the camera module lens. The system also includes a diffuser configured to diffuse the transmission light and a diffusive black material coupled to the diffuser. The diffusive black material is configured to pass through at least some of the transmission light while blocking reflections of the scattering light.