A light sheet fluorescence microscope includes a light source configured to emit excitation light suitable for inducing fluorescent light emitted from a specimen, a detector configured to detect the fluorescent light from the specimen, and an optical system configured to illuminate the specimen with a light sheet formed from the excitation light, and to guide the fluorescent light from the illuminated specimen to the detector. The optical system includes an objective facing the specimen, the objective being configured to collect the fluorescent light emitted from the specimen. The light source is further configured to emit manipulation light suitable for photomanipulating the specimen. The optical system is further configured to direct the manipulation light through a spatially limited sub-area of an entrance pupil of the objective onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet.

A microscope for examining a sample received in a sample carrier having a lid includes: a sample chamber for receiving the sample carrier; a microscope stage arranged below the sample chamber, the microscope stage being arranged to have the sample carrier arranged thereon; and a sample carrier handling device that is at least partially arranged within the sample chamber and that removes the lid from the sample carrier to provide access to the sample.

A microscope for examining a sample received in a sample carrier having a lid includes: a sample chamber for receiving the sample carrier; a microscope stage arranged below the sample chamber, the microscope stage being arranged to have the sample carrier arranged thereon; and a sample carrier handling device that is at least partially arranged within the sample chamber and that removes the lid from the sample carrier to provide access to the sample.

The purpose of the present invention is to collect a plurality of microscopic objects dispersed in a liquid by light irradiation, and also trap them. A collecting device for bacteria collects a plurality of bacteria dispersed in a sample liquid. The collecting device is provided with a laser beam source that emits laser beam and a honeycomb polymer film constituted so as to be able to hold the liquid. Walls prescribing pores for trapping the plurality of bacteria dispersed in the liquid are formed on the honeycomb polymer film, and also a thin film that includes a material for converting light from the laser beam source to heat is formed on the honeycomb polymer film. The thin film heats the liquid of the sample through the conversion of the laser beam from the laser beam source to heat, thereby causing a convection in the liquid.

The purpose of the present invention is to collect a plurality of microscopic objects dispersed in a liquid by light irradiation, and also trap them. A collecting device for bacteria collects a plurality of bacteria dispersed in a sample liquid. The collecting device is provided with a laser beam source that emits laser beam and a honeycomb polymer film constituted so as to be able to hold the liquid. Walls prescribing pores for trapping the plurality of bacteria dispersed in the liquid are formed on the honeycomb polymer film, and also a thin film that includes a material for converting light from the laser beam source to heat is formed on the honeycomb polymer film. The thin film heats the liquid of the sample through the conversion of the laser beam from the laser beam source to heat, thereby causing a convection in the liquid.

An optical assembly in a laser scanning microscope, having an optical scanning unit providing a first pupil plane, a first beam deflecting device, made of a first scanner arranged on the first pupil plane, for scanning excitation radiation in a first coordinate direction, a first focusing device generating a second pupil plane, optically conjugated to the first pupil plane, and a second beam deflecting device for deflecting the excitation radiation. The second deflecting device is arranged on the second pupil plane. A second focusing device to generate a third pupil plane, is optically conjugated to the first pupil plane and the second pupil plane. A third beam deflecting device is arranged on the third pupil plane, and a variable beam deflecting device is provided to switch an optical beam path between a first beam path and a second beam path.

Systems and methods are provided for Quantitative Phase Microscopes (QPM) having laser systems including one or more of laser scissors and laser tweezers. In one embodiment, the system includes one or more structural elements, such as a stage and dichroic plate for operation of a QPM with laser scissors/tweezers. Another embodiment is directed to a method of operating a QPM system having laser scissors/tweezers. One or more solutions are provided for biodmedical applications of a QPM system including simulation and analysis of trauma on cellular structures and organelles. Processes are also provided for simulation and analysis of traumatic injury, including imaging and analysis of astrocytes.

Systems and methods are provided for Quantitative Phase Microscopes (QPM) having laser systems including one or more of laser scissors and laser tweezers. In one embodiment, the system includes one or more structural elements, such as a stage and dichroic plate for operation of a QPM with laser scissors/tweezers. Another embodiment is directed to a method of operating a QPM system having laser scissors/tweezers. One or more solutions are provided for biodmedical applications of a QPM system including simulation and analysis of trauma on cellular structures and organelles. Processes are also provided for simulation and analysis of traumatic injury, including imaging and analysis of astrocytes.

Provided herein are systems and methods for simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system. The microscope can comprise in part an imaging light source and a stimulation light source. Light from the imaging light source and the stimulation light source can be spectrally separated to reduce cross talk between the stimulation light and the imaging light.

Provided herein are systems and methods for simultaneous imaging and stimulation using a microscope system. The microscope system can have a relatively small size compared to an average microscope system. The microscope can comprise in part an imaging light source and a stimulation light source. Light from the imaging light source and the stimulation light source can be spectrally separated to reduce cross talk between the stimulation light and the imaging light.