Methods and systems for controlling a surgical microscope. Moveable optics of the surgical microscope are controlled using two sets of control parameters, to reduce jitter and image instability. Shifts in the image due to changes in temperature or due to the use of optical filter can also be compensated. Misalignment between the mechanical axis and the optical axis of the surgical microscope can also be corrected.

A medical/surgical microscope with two cameras configured to capture two dimensional images of specimens being observed. The medical/surgical microscope is secured to a control apparatus configured to adjust toe-in of the two cameras to insure the convergence of the images. The medical/surgical microscope includes a computer system with a non-transitory memory apparatus for storing computer program code configured for digitally rendering real-world medical/surgical images. The medical/surgical microscope has an illumination system with controls for focusing and regulating the lighting of a specimen. The medical/surgical microscope is configured for real-time video display with the function of recording and broadcasting simultaneously during surgery.

Methods and systems are provided for synchronizing image capture at a multi-detector imaging system. In one example, a method includes coordinating cycling of each microscope assembly of the multi-detector imaging system through a selection of illumination channels, each microscope assembly configured to obtain an image of a portion of one of more than one microplate wells simultaneously, to generate complete images of the more than one microplate wells concurrently.

Methods and systems are provided for an imaging system. In one example, the imaging system includes a plurality of microscope assemblies arranged radially around a central axis of the imaging system, coupled to vertically oriented plates with a set of objectives arranged at tops of the plurality of microscope assemblies and wherein the plates are oriented to extend away from the central axis in a radial direction to form an x-shaped configuration.

Methods and systems are provided for a microscope assembly. In one example, the microscope assembly include an objective arranged at a top of a plate and aligned with a first side of the plate and a tube lens positioned below the objective along the first side of the plate and spaced away from the objective. The assembly further includes a laser auto-focus oriented parallel with a height of the plate and a light source coupled to a central region of the front face of the plate, between the tube lens and the laser auto-focus.

A mobile phone-based miniature microscopic image acquisition device, and image stitching and recognition methods are provided. The acquisition device comprises a support, wherein a mobile phone fixing table is provided on the support. A microscope head is provided below a camera of a mobile phone. A slide holder is provided below the microscope head, and an lighting source is provided below the slide holder. A scanning movement is performed between the slide holder and the microscope head along X and Y axes, so that images of a slide are acquired into the mobile phone. The slide sample images acquired into the mobile phone can be stitched and recognized, and can be uploaded to the cloud to be processed by cloud AI, thereby significantly improving the accuracy and efficiency of cell recognition, greatly reducing the medical cost, and ensuring more remote medical institutions can apply such technology for diagnosis.

A machine learning based imaging system comprises an imaging apparatus for attachment to an imaging sensor of a mobile computing apparatus such as camera of a smartphone. A machine learning (or AI) based analysis system is trained on images captured with the imaging apparatus attached, and once trained may be deployed with or without the imaging apparatus. The imaging apparatus comprise an optical assembly that may magnify the image, an attachment arrangement and a chamber or a wall structure that forms a chamber when placed against an object. The inner surface of the chamber is reflective apart and has a curved profile to create uniform lighting conditions on the one or more objects being imaged and uniform background lighting to reduce the dynamic range of the captured images.

A cell observation system according to the present invention includes: a first image-acquisition device disposed in an incubator and acquires a first image of cells in a culturing vessel; a second image-acquisition device disposed outside the incubator; a processing device connected to the first image-acquisition device and the second image-acquisition device; and a display displays at least a region of the first image, as well as the second image. The second image-acquisition device includes a second image-acquisition unit that acquires a second image of the interior of the culturing vessel, that removed from the incubator. The processing device extracts target cells in the first image, calculates positions at which the extracted target cells are present and stores the positions in the memory, and causes the display to display the positions at which the target cells are present in a superimposed manner on an indication indicating the culturing vessel.

An observation system includes an observation apparatus including a housing having an arrangement surface for placement of a sample, the sample including a culture medium, and an external illumination unit which is disposed outside the housing and includes at least one light source configured to emit illumination light. At least a part of the arrangement surface is formed of a transparent member having an optically transparent property. The observation apparatus includes an imaging unit which is provided in the housing and includes an image sensor configured to image, via the transparent member, the sample illuminated by the illumination light from the external illumination unit to acquire images of at least three colors.

An arrangement of a lens less imaging microscopy system having an antimicrobial and anti-bacterial surface. The system provides a modular layout, i.e., having exchangeable or interchangeable modules along with autoclave ability of individual modules. The lens less imaging system may include a light engine module, sample containing specific module, camera module, chip dock module that are swappable, respectively. The lens less imaging system may also include a software module that allows recognition of attachments and detachments of the various modules. The lens less imaging system has higher flexibility and affordability due to the swappable modules.