Examples relate to apparatuses, methods and computer programs for controlling a microscope system, and to a corresponding microscope system. An apparatus for controlling a microscope system comprises an interface for communicating with a camera module. The camera module is suitable for providing camera image data of a head of a user of the microscope system. The apparatus comprises a processing module configured to obtain the camera image data from the camera module via the interface. The processing module is configured to process the camera image data to determine information on an angular orientation of the head of the user relative to a display of the microscope system. The processing module is configured to provide a control signal for a robotic adjustment system of the microscope system based on the information on the angular orientation of the head of the user.

Quantitative methods for optimizing the permeabilization of cellular tissues for spatial transcriptomics are provided. Also provided is an instrument for quantitatively optimizing the permeabilization of cellular tissues used for spatial transcriptomics.

Devices and methods to measure cells and/or tissue's contractile force are disclosed. Included is a mount with rigid, and non-rigid posts sized to flex. Determined is force exerted by contractile cells and tissues in a multiwell plate. The device is designed to fit inside individual wells with posts directed downwards. Posts are attached to a 3D printed circular mount with tabs for depth within the well. The mount has a window for medium changes while the device is positioned inside the well. The cells are seeded within a hydrogel. As the hydrogel condenses, cells/tissue wrap around the post's outside pulling non-rigid post toward rigid post. Inverted light microscope is used to determine deflection of non-rigid post inside the multiwell plate. Movement of the non-rigid post is measured using an acrylic ruler on an underside of the multiwell plate. Contractile forces of cells/tissue are determined using cantilever mechanics.

In a method for processing microscope images, at least one microscope image is provided as input image for an image processing algorithm. An output image is created from the input image by means of the image processing algorithm. The creation of the output image comprises adding low-frequency components for representing solidity of image structures of the input image to the input image, wherein the low-frequency components at least depend on high-frequency components of these image structures and wherein high-frequency components are defined by a higher spatial frequency than low-frequency components. A corresponding computer program and microscope system are likewise described.

A system for performing adaptive focusing of a microscopy device comprises a microscopy device configured to acquire microscopy images depicting cells and one or more processors executing instructions for performing a method that includes extracting pixels from the microscopy images. Each set of pixels corresponds to an independent cell. The method further includes using a trained classifier to assign one of a plurality of image quality labels to each set of pixels indicating the degree to which the independent cell is in focus. If the image quality labels corresponding to the sets of pixels indicate that the cells are out of focus, a focal length adjustment for adjusting focus of the microscopy device is determined using a trained machine learning model. Then, executable instructions are sent to the microscopy device to perform the focal length adjustment.

The present disclosure relates to a reflective FPM using a parabolic mirror, and particularly to a reflective FPM using a parabolic mirror including: a first illuminator having a first panel that is provided with numerous LED light sources and composed of a first LED array irradiating a plurality of first LED beams to a measurement object sequentially at different angles through an objective lens; a second illuminator having a second panel that is provided with numerous LED light sources and composed of a second LED array irradiating a plurality of second LED beams to the measurement object sequentially at different angles, following irradiation from the first illuminator; a parabolic mirror reflecting each of second beams generated from the second illuminator, allowing being incident on the measurement object; a lens configured to collect a beam from the measurement object to which the first and second LED beams were irradiated; and a photodetector receiving light from the lens and acquires images for each of a plurality of first and second beams.

A microscope auxiliary apparatus attachable to a microscope includes an object movable portion for moving an object in an optical axis direction of the microscope, first and second operating unit movable portions for respectively moving, in the optical axis direction, first and second operating units for operating the object, a movement instructing unit for instructing the object movable portion or the first or second operating unit movable portions to move, and a switching unit for switching a mode to first and second modes. The first mode moves one of the movable portions in the optical axis direction according to an instruction from the movement instructing unit. The second mode links movements of at least two of the movable portions and moves the at least two in the optical axis direction according to the instruction from the movement instructing unit.

The invention discloses a microlens and an application thereof, wherein the microlens is a lipid particle. The microlens prepared by the invention is simple in preparation and extraction methods, and does not need extra processing. The lipid particle is capable of serving as an optical element inside a cell to exert an optical function and has a complete biocompatibility; and meanwhile, the lipid particle is naturally generated inside the cell, and has a natural position-close relationship with a microstructure inside the cell, and is capable of collecting and re-positioning an optical signal of the microstructure in a near field, so that an imaging quality of the cell microstructure of an optical microscope is effectively improved.

Provided are an image acquisition device which increases a small depth of field of an objective lens to acquire a high depth of field image and an image acquisition method using the same. The image acquisition device according to an exemplary embodiment of the present disclosure is an image acquisition device which acquires an image of a subject including an image collection unit; and an objective lens unit disposed below the image collection unit, and the image collection unit generates an image in which Z-axis signals are superposed within a range corresponding to a thickness of the subject, in an area to be captured of the subject.

A microscopy includes multiple cameras working together to capture image data of a sample having a group of organisms distributed over a wide area, under the influence of an excitation instrument. A first processor is coupled to each camera to process the image data captured by the camera. Outputs from the multiple first processors are aggregated and streamed serially to a second processor for tracking the organisms. The presence of the multiple cameras capturing images from the sample, configured with 50% or more overlap, can allow 3D tracking of the organisms through photogrammetry.