Systems and methods herein provide improved, high-throughput multiphoton imaging of thick samples with reduced emission scattering. The systems and methods use structured illumination to modify the excitation light. A reconstruction process can be applied to the resulting images to recover image information free of scattering. The disclosed systems and methods provide high throughput, high signal-to-noise ratio, and high resolution images that are depth selective.

A two-photon stimulated emission depletion composite microscope, comprising a two-photon imaging unit (100) and an STED imaging unit (200), wherein the two-photon imaging unit (100) can be used for a relatively thick sample, and the STED super-resolution imaging unit can be used for a region of interest on a surface of a sample, and the microscope makes light spots generated by an excitation light and a depletion light after being focused by an objective lens (OL) accurately coincide in a three-dimensional distribution. The two-photon stimulated emission depletion composite microscope (10) integrates two functions of STED imaging and two-photon imaging and makes the two types of light spots generated by an excitation light and a depletion light after being focused by an objective lens accurately coincide in a three-dimensional distribution, thereby providing a powerful tool for cutting-edge biomedical research.

A non-linear optical pumping detection apparatus and a non-linear optical absorption cross-section measurement method, which can simultaneously measure degenerate and non-degenerate two-photon absorption cross-section spectra. The measurement process is automatic, efficient and fast. The working wavelength band is from 380 nm to near infrared 1064 nm, and the non-linear performance measurement of the super-continuous wide spectra can be realized. A zoom optical system with a larger entrance pupil diameter is adopted as a weak signal acquisition lens. So the weak signal can be effectively extracted from background noise. Meanwhile, the mean square root diameter of an on-axis image point of the zoom optical system is 100 to 150 microns, the divergence angle 2α of the on-axis image point is 30.6 degrees, which well match the optical fiber coupling condition, thereby improving the coupling efficiency of the space light coupling into the optical fiber, and greatly improving the measurement sensitivity.

A method of imaging a sample providing light from a light source, directing the provided light into an extended focus, scanning the extended focus across a wavefront modulating element that modulates amplitudes of the light along the extended focus, providing the modulated light to the sample, detecting light emitted from the sample in response to excitation by the modulated light, and generating an image of the sample based on the detected fluorescence emission light.

For correcting aberration-induced imaging errors of an optical system which includes an objective (14) and an adaptive optic (18), light (5) and a sample (20) are selected such that the light (5), in acting upon the sample (20), reduces a measurement signal (28) from the sample (20), wherein a relative variation of the measurement signal (28) depends on the intensity of the light (5). The measurement signal (28) from a focal area of the optical system in the sample (20) is registered over a first and a later second period of time (38, 37) to determine a first measurement value and a second measurement value. Over a third period of time (39) which overlaps with the first and/or the second period of time, the light (5) is focused into the focal area by means of the optical system. A measure value for the relative variation of the measurement signal (28) is determined from the first and the second measurement values and used in controlling the adaptive optic (18) as a metric to be optimized.

A system and method for adaptive illumination, the imaging system comprising an excitation source having a modulator, which generates a pulse intensity pattern having a first wavelength when the excitation source receives a modulation pattern. The modulation pattern is a data sequence of a structural image of a sample. An amplifier of the imaging system is configured to receive and amplify the pulse intensity pattern from the modulator. A frequency shift mechanism of the imaging system shifts the first wavelength of the pulse intensity pattern to a second wavelength. A laser scanning microscope of the imaging system receives the pulse intensity pattern having the second wavelength.

Aspects of the subject disclosure may include, for example, identifying a request to facilitate communications between first and second processing nodes, determining that the communications are to be established via quantum teleportation between, and identifying a network path comprising a first path segment to obtain a quantum channel, wherein quantum entanglement is established between the first and second processing nodes based on transportation of a first quantum entangled object via the quantum channel. A classical communication channel is facilitated between the first and second processing nodes, adapted to exchange between the nodes, quantum state information of a measurement performed upon the first quantum entangled object. Information is exchanged between the first and second processing nodes via the quantum channel according to the transported first quantum entangled object and the exchanged quantum state information. Other embodiments are disclosed.

Near-infrared pulses are emitted from a pulsed light source. A scanner directs the near-infrared pulses as scanned light. An optical element directs the scanned light into the eye. The scanned light is scanned in two dimensions to form an image on the eye. Photon-pairs of the near-infrared pulses deliver a photon energy that is perceived as visible light.

A device component assembly including an upper support plate (USP) of glass and a lower support plate (LSP) of metal affixed to the USP, and a manufacturing method are provided. The USP and the LSP include openings of different shapes and sizes. The LSP includes gaps cut in different directions for reducing thermal expansion and tension generated during a temperature shift. Device components including optical, mechanical, electric, electronic, and optoelectronic components are mutually optically aligned and mounted on the USP and/or the LSP based on component requirements. The device components are mounted on the LSP through the openings of the USP. The optical components are affixed to the support plate(s) using a fastening material. One or more heat transfer members are affixed to the LSP for mounting the device component(s) thereon, after mutual optical alignment therebetween. The device component assembly is integrated in an optical or optoelectronic module or system.

A system and method for adaptive illumination, the imaging system comprising an excitation source having a modulator, which generates a pulse intensity pattern having a first wavelength when the excitation source receives a modulation pattern. The modulation pattern is a data sequence of a structural image of a sample. An amplifier of the imaging system is configured to receive and amplify the pulse intensity pattern from the modulator. A frequency shift mechanism of the imaging system shifts the first wavelength of the pulse intensity pattern to a second wavelength. A laser scanning microscope of the imaging system receives the pulse intensity pattern having the second wavelength.