A method for performing a procedure on a patient includes acquiring a three-dimensional image of a location of interest on the patient and a two-dimensional image of the location of interest can be acquired. A computer system can relate the three-dimensional image with the two-dimensional image to form a holographic image dataset. The computer system can register the holographic image dataset with the patient. The augmented reality system can render a hologram based on the holographic image dataset from the patient. The hologram can include a projection of the three-dimensional image and a projection of the two-dimensional image. The practitioner can view the hologram with the augmented reality system and perform the procedure on the patient. The practitioner can employ the augmented reality system to visualize a point on the projection of the three-dimensional image and a corresponding point on the projection of the two-dimensional image during the procedure.

A method for performing a procedure on a patient includes acquiring a three-dimensional image of a location of interest on the patient and a two-dimensional image of the location of interest can be acquired. A computer system can relate the three-dimensional image with the two-dimensional image to form a holographic image dataset. The computer system can register the holographic image dataset with the patient. The augmented reality system can render a hologram based on the holographic image dataset from the patient. The hologram can include a projection of the three-dimensional image and a projection of the two-dimensional image. The practitioner can view the hologram with the augmented reality system and perform the procedure on the patient. The practitioner can employ the augmented reality system to visualize a point on the projection of the three-dimensional image and a corresponding point on the projection of the two-dimensional image during the procedure.

Provided are a method and an apparatus for printing a hologram by using a mask. The hologram printing method according to an embodiment generates a hologram fringe pattern, splits the hologram fringe pattern on a hogel basis, generates the split hogels, masks a part of the generated hogel, and prints the masked hogel on a hologram medium. Accordingly, an empty space which occurs between hogels when a hologram is printed is prevented from being generated by using a mask, so that a fill factor can be effectively enhanced, and eventually, image quality of a hologram can be enhanced.

In order to quantitatively characterize biological objects, for example individual cells, a stimulus is applied to a biological object (8) in a contactless fashion. A measurement and a further measurement are performed on the biological object (8) in order to ascertain a response of the biological object (8) to the stimulus, wherein the measurement and the further measurement comprise detecting Raman scattering on and/or in the biological object (8) and/or capturing data using digital holographic microinterferometry (DHMI). The biological object (8) is characterized according to a result of the measurement and is sorted if needed. The stimulus can be applied by means of a laser beam that creates optical tweezers or an optical trap, by means of ultrasonic waves or an electric or magnetic radio frequency field.

A method for analyzing microorganisms arranged in a sample is provided, the sample including a viability marker to modify an optical property of the microorganisms in different ways depending on whether they are dead or alive, the method including illumination of the sample and acquisition of an image of the latter by an image sensor, the image sensor then being exposed to an exposure light wave; determining positions of different microorganisms from the acquired image; applying a propagation operator to calculate at least one characteristic value of the exposure light wave at each radial position and at a plurality of distances from the detection plane representing a change in the characteristic value between the image sensor and the sample; and identifying living microorganisms according to each profile.

According to an aspect of the present inventive concept there is provided an imaging system for imaging of a sample, comprising a light source, an interference filter and a detector, the light source generates illumination light of a single wavelength to induce elastic scattering of the light by the sample, the interference filter selectively reduces transmittance of light having an incident angle on the interference filter corresponding to non-scattered light, the detector is configured to detect a two-dimensional representation of the elastically scattered light transmitted by the interference filter.

A method for observing a sample (10), the sample lying in a plane of the sample defining radial coordinates, the method comprising the following steps: a) illuminating the sample using a light source (11), able to emit an incident light wave (12) that propagates toward the sample along a propagation axis (Z); b) acquiring, using an image sensor (16), an image (I.sub.0) of the sample (10), said image being formed in a detection plane (P.sub.0), the sample being placed between the light source (11) and the image sensor (16), such that the incident light wave sees an optical path difference, parallel to the propagation axis (Z), by passing through the sample; c) processing the image acquired by the image sensor;
wherein the processing of the acquired image comprises taking into account vectors of parameters, respectively defined at a plurality of radial coordinates, in the plane of the sample, each vector of parameters being associated with one radial coordinate, and comprising a term representative of an optical parameter of the sample, at least one optical parameter being an optical path difference induced by the sample at the radial coordinate, the vectors of parameters describing the sample.

The present disclosure provides a conference device, a method of controlling the conference device, and a computer storage medium. The conference device includes a display, an image sensor, a holographic projector, and a controller configured to identify, by using an image data from the image sensor, a modification action performed at a target location for a holographic image projected by the holographic projector, modify holographic projection data based on the modification action, and convert modified holographic projection data into modified two-dimensional imaging data.

The present disclosure provides a conference device, a method of controlling the conference device, and a computer storage medium. The conference device includes a display, an image sensor, a holographic projector, and a controller configured to identify, by using an image data from the image sensor, a modification action performed at a target location for a holographic image projected by the holographic projector, modify holographic projection data based on the modification action, and convert modified holographic projection data into modified two-dimensional imaging data.

An apparatus and method to produce a hologram of an object includes an electromagnetic radiation assembly configured to receive a received electromagnetic radiation, such as light, from the object. The electromagnetic radiation assembly is further configured to diffract the received electromagnetic radiation and transmit a diffracted electromagnetic radiation. An image capture assembly is configured to capture an image of the diffracted electromagnetic radiation and produce the hologram of the object from the captured image.