An imaging flow cytometer device includes a housing holding a multi-color illumination source configured for pulsed or continuous wave operation. A microfluidic channel is disposed in the housing and is fluidically coupled to a source of fluid containing objects that flow through the microfluidic channel. A color image sensor is disposed adjacent to the microfluidic channel and receives light from the illumination source that passes through the microfluidic channel. The image sensor captures image frames containing raw hologram images of the moving objects passing through the microfluidic channel. The image frames are subject to image processing to reconstruct phase and/or intensity images of the moving objects for each color. The reconstructed phase and/or intensity images are then input to a trained deep neural network that outputs a phase recovered image of the moving objects. The trained deep neural network may also be trained to classify object types.

A display panel (1) comprising a body of optical material, the body having at least one optical image recorded therein in an encoded manner, wherein the image is selectively reconstructable and viewable (5, 6, 7) by illuminating the panel (1) using at least one light source (3a, 3b, 3c) under selected illumination conditions, wherein the image is reconstructable and viewable (5, 6, 7) such that at least one first optical property or parameter of the reconstructed image (e.g. its geometry, position in space, colour, its dynamic appearance) is selectable in value from amongst variable values of the at least one first optical property or parameter, or whose value is actively modifiable over time, as a function of or in dependence on the value of at least one second optical property or parameter of the illumination conditions of the at least one light source (e.g. its/their position(s) or spacing(s) relative to the panel (1), its/their colour, brightness/optical intensity, polarisation, direction of light ray propagation, application of a scanning technique to illuminate the panel (1)) which is selectable from amongst variable values thereof or which is actively modifiable in value over time.

A holographic imaging system may include an optical source configured to output a source beam, a splitter configured to split the source beam into a reference beam and an object beam that is incident on a target to form a scattered object beam, and a pre-filter comprising a telecentric lens and a spatial filter. The pre-filter may be configured to receive the scattered object beam and filter diffuse light from the scattered object beam to form a filtered scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, and an imaging array configured to receive the interference beam and generate raw holographic data based on the interference beam.

A system for generating holographic images or videos comprising a camera array, a plurality of processors, and a central computing system. A method for generating holographic images can include receiving a set of images and processing the images.

An imaging system for imaging a fluid sample includes a light source configured to generate a beam of light, an angled element disposed along an optical path of the beam of light, and a sample cartridge holder configured to receive a sample cartridge and configured to hold the sample cartridge in a first position in which an imaging region of the sample cartridge is disposed along the optical path. The system further includes a sensor configured to capture the beam of light after it passes through the angled element and the imaging region of the sample cartridge. The imaging region of the sample cartridge is configured to receive the sample fluid. A sample cartridge having a cover plate and a fluidics layer is also disclosed. The fluidics layer includes an opening, a fluid channel, and an imaging region configured to receive a whole blood sample.

The invention relates to a digital holography method for detecting the vibration amplitude of an object (15) having a vibration frequency ω, comprising: generating object illumination waves (W.sub.t) and reference waves (W.sub.LO); acquiring interferograms between the reference wave (W.sub.LO) and a signal wave (W.sub.s) by means of a bandwidth ω s detector (19), the reference wave comprising two components E.sub.LO1, E.sub.LO1 of frequencies ω.sub.1, ω.sub.2 that are respectively staggered in relation to the laser frequency ω.sub.L by a quantity δ.sub.1=γ.sub.1ω.sub.s and δ2=qω+γ2ω.sub.s, where q is an integer and −0.5≦γ1, γ.sub.2≦0.5; and calculating the vibration amplitude of the object from the optical beats spectrum deduced from the complex amplitude of an interferogram.

A vehicle such as a helicopter may scan a scene using a transmitter mounted on a rotating part like a rotor and a receiver mounted on a body of the vehicle. Based on a Doppler shift caused by the rotation of the rotating part, patterns may be recorded and used to develop a holographic image of the scene.

A holographic imaging system may include an optical source configured to output a source beam and a splitter configured to split the source beam into a reference beam and an object beam that may be incident on a target to form a scattered object beam. The system may also include a combiner configured to combine the filtered scattered object beam with the reference beam to form an interference beam, an imaging array configured to receive the interference beam and generate frames of raw holographic data based on measurements of the interference beam over time, and an image data processor. The image data processor may be configured to receive the frames of raw holographic data from the imaging array, remove data components within the frames that are associated with the particle motion having a motion frequency that is less than a movement frequency threshold to form conditioned raw holographic data, and generate an image based on the conditioned raw holographic data.

The present invention relates to an automatic real-time label-free holography-activated sorting of the cell's technique. The technique provides high-discriminative power on the level of the individual cell. The technique includes rapid automated cell processing during cell visualization and flow, with high discriminative power on the level of the individual cell. The technique may be useful in detection of cancer and to identify different stages of oncogenesis.

A system for generating holographic images or videos comprising a camera array, a plurality of processors, and a central computing system. A method for generating holographic images can include receiving a set of images and processing the images.