A musical sound signal generation device continuously connects any one of a connected zeroth delay unit and a connected second delay unit to a fractional delay block and connects at least any one of a new zeroth delay unit and a new second delay unit to at least any one of the fractional delay block other than the fractional delay block connected to a new first delay unit in response to setting any one of the connected zeroth delay unit and the connected second delay unit as the new first delay unit, setting a delay unit in a preceding stage of the new first delay unit as the new zeroth delay unit, and setting a delay unit in a subsequent stage of the new first delay unit as the new second delay unit in accordance with a change in a designated tone pitch.