The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.

A multi-way valve has an upper part, a lower part and a central part connected to the upper part via an interposing first membrane and the lower part via an interposing second membrane, wherein the upper and lower parts contain recesses on the upper and lower part faces facing the membranes, where a control fluid is introducible into the recesses, the central part contains a pair of openings in regions opposite the recesses on the central part faces lying adjacent to the membranes, where while introducing the control fluid into a recess, the membrane closes respective opposite openings in the central part, and where the membrane otherwise recedes into the recess, thereby releasing the opposite openings, where valve connections are mounted on the upper or lower part such that the central part can have a very thin design while preventing dead regions in the channel systems.

A multi-way valve has an upper part, a lower part and a central part connected to the upper part via an interposing first membrane and the lower part via an interposing second membrane, wherein the upper and lower parts contain recesses on the upper and lower part faces facing the membranes, where a control fluid is introducible into the recesses, the central part contains a pair of openings in regions opposite the recesses on the central part faces lying adjacent to the membranes, where while introducing the control fluid into a recess, the membrane closes respective opposite openings in the central part, and where the membrane otherwise recedes into the recess, thereby releasing the opposite openings, where valve connections are mounted on the upper or lower part such that the central part can have a very thin design while preventing dead regions in the channel systems.

Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.

Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.