Embodiments of the present disclosure pertain to methods of detecting a thiol in an environment by exposing the environment to a probe molecule that contains a marker and a thiol responsive group. The thiol responsive group reversibly reacts with the thiol in the environment to form a probe-thiol adduct. This in turn causes a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct, which can then be correlated to the presence of the thiol in the environment. The correlation can occur by quantifying the thiol concentration in the environment. In addition, thiol detection can occur in real-time. Further embodiments of the present disclosure pertain to probe molecules that are utilized for detecting a thiol in an environment. In some embodiments, the probe molecule includes a marker and a thiol responsive group. In some embodiments, the probe molecule also includes an organelle targeting moiety.

A method uses an Dioleoylphosphatidylcholine (DOPC) surfactant based bio film that reacts with a material in a known manner, and a device that utilizes such a biofilm, to detect material of interest is provided. The principles of the present invention are particularly useful in detecting/measuring a material.

Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort including centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using 9-oxo-HODE as biomarker.

Technologies are generally described for gas filtration and detection devices. Example devices may include a graphene membrane and a sensing device. The graphene membrane may be perforated with a plurality of discrete pores having a size-selective to enable one or more molecules to pass through the pores. A sensing device may be attached to a supporting permeable substrate and coupled with the graphene membrane. A fluid mixture including two or more molecules may be exposed to the graphene membrane. Molecules having a smaller diameter than the discrete pores may be directed through the graphene pores, and may be detected by the sensing device. Molecules having a larger size than the discrete pores may be prevented from crossing the graphene membrane. The sensing device may be configured to identify a presence of a selected molecule within the mixture without interference from contaminating factors.

Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Methods and kits for diagnosis and staging of shock, and especially non-septic shock are presented in which protease activities and/or volatile compounds are measured from a biological sample to so identify and/or stage shock.

Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

A portable electronic device comprises a chemical sensor that is sensitive to a concentration of a chemical analyte and at least two auxiliary sensors that are sensitive to parameters that are different from the concentration of the chemical analyte. The portable electronic device comprises a control device that receives signals from the chemical sensor and from the auxiliary sensors at a plurality of points in time distributed over a measurement period and correlates the time dependencies of these signals to obtain a corrected reading of the first chemical sensor. The portable electronic device may be employed for breath analysis.

Methods and kits for diagnosis and staging of shock, and especially non-septic shock are presented in which protease activities and/or volatile compounds are measured from a biological sample to so identify and/or stage shock.