Medical Instrumentation Utilizing Narrowband Imaging

Abstract

An illumination source comprised of individual light emitting diodes (LEDs) specifically formed to operate at wavelengths associated with the absorption spectrum of certain biomolecule(s) of interest present in the region of the body being examined.

Advantageously, LEDs may be configured to generate a high intensity, narrowband beam that is well-suited for these medical imaging purposes where the ability to provide a proper diagnosis relies on the ability to create a high contrast image for review by the medical professionals. The inventive illumination source may also include a conventional white light source that is used as before for general observation purposes, with the one or more narrowband LEDs activated when there is a need to create a high contrast image of a particular ROI.

Claims

1. An illumination source useful in performing digital imaging in conjunction with medical scopic instrumentation, the illumination source comprising at least one narrowband first-wavelength LED operating at a first center wavelength λ.sub.1 associated with a first absorbance peak of an anatomical region of interest (ROI) under study; and at least one narrowband second-wavelength LED operating at a second center wavelength λ.sub.2 associated with a second absorbance peak of the anatomical region of interest (ROI) under study, the energizing of the at least one narrowband first-wavelength LED and the at least one narrowband second-wavelength LED are controlled in a manner that creates a high contrast digital image of the ROI.

2. The illumination source as defined in claim 1 wherein the illumination source further comprises a white light source for alternative illumination of the ROI.

3. The illumination source as defined in claim 1 wherein the source further comprises a photoreceiving element positioned to receive reflected light from the ROI.

4. The illumination source as defined in claim 3 wherein the photoreceiving element comprises a combination of a CMOS detector and wavelength-dependent filters.

5. The illumination source as defined in claim 1 wherein each narrowband LED exhibits a FWHM of no greater than 30 nm.

6. The illumination source as defined in claim 1 wherein the at least one narrowband first-wavelength LED comprises a plurality of separate LEDs, disposed to illuminate selected areas of the ROI.

7. The illumination source as defined in claim 1 wherein the at least one narrowband second-wavelength LED comprises a plurality of separate LEDs, disposed to illuminate selected areas of the ROI.

8. The illumination source as defined in claim 1 wherein the illumination source further comprises a white light source, used for an examination of a general part of the anatomy.

9. The illumination source as defined in claim 1 wherein the LEDs of different wavelengths are disposed proximate to each other in an array at defined locations around a periphery of a centrally-disposed photoreceiving element.

10. The illumination source as defined in claim 1 wherein the illumination source is utilized in conjunction with scopic system for viewing vasculature, the first and second center wavelengths selected to be proximate to absorbance peaks of hemoglobin.

11. The illumination source as defined in claim 10 wherein the at least one narrowband first-wavelength LED operates at a wavelength λ.sub.1≈540 nm and is referred to as an at least one green LED, and the at least one narrowband second-wavelength LED operates at a wavelength λ.sub.2≈415 nm and is referred to as an at least one blue LED.

12. The illumination source as defined in claim 11 wherein the at least one green LED comprises a plurality of green LEDs all operating at a wavelength λ.sub.2≈540 nm.

13. The illumination source as defined in claim 11 wherein the at least one blue LED comprises a plurality of blue LEDs all operating at a wavelength λ.sub.2≈415 nm.

14. The illumination source as defined in claim 10 wherein the at least one green LED comprises a plurality of green LEDs all operating at a wavelength λ.sub.1≈540 nm, and the at least one blue LED comprises a plurality of blue LEDs all operating at a wavelength λ.sub.2≈415 nm.

15. The illumination source as defined in claim 1 wherein the illumination source is utilized in conjunction with a dermatoscope, the first and second center wavelengths selected to be proximate to absorbance peaks of skin pigments.

16. The illumination source as defined in claim 15 wherein the at least one narrowband first-wavelength LED operates at a wavelength λ.sub.1≈625 nm and is referred to as an at least one red LED, and the at least one narrowband second-wavelength LED operates at a wavelength λ.sub.2≈580 nm and is referred to as an at least one yellow LED.

17. The illumination source as defined in claim 16 wherein the at least one red LED comprises a plurality of red LEDs all operating at a wavelength λ.sub.1≈625 nm.

18. The illumination source as defined in claim 16 wherein the at least one yellow LED comprises a plurality of yellow LEDs all operating at a wavelength λ.sub.2≈580 nm.

19. The illumination source as defined in claim 16 wherein the at least one red LED comprises a plurality of red LEDs all operating at a wavelength λ.sub.1≈625 nm, and the at least one yellow LED comprises a plurality of yellow LEDs all operating at a wavelength λ.sub.2≈580 nm.

20. An illumination source for use in performing digital imaging in conjunction with medical instrumentation, the illumination source including at least one narrowband LED operating at a center wavelength associated with an absorbance peak of a biomolecule present in an anatomical region of interest (ROI) under study, enhancing a contrast between a specific set of features in the ROI and surrounding material, generating a high contrast digital image of the ROI.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Referring now to the drawings, where like element include like reference numbers in several views:

[0014] FIG. 1 depicts examples of medical instrumentation used to perform optical imaging;

[0015] FIG. 2 is a simplified isometric view of an exemplary illumination source formed in accordance with the present invention;

[0016] FIG. 3 is a block diagram side view of the illumination source of FIG. 2;

[0017] FIG. 4 is a front view of an exemplary arrangement of narrowband LEDs within the inventive illumination source;

[0018] FIG. 5 is a front view of an alternative arrangement of narrowband LEDs within the inventive illumination source;

[0019] FIG. 6 shown yet another arrangement of narrowband LEDs within an illumination source formed in accordance with the principles of the present invention;

[0020] FIG. 7 is a photographic reproduction of a prior art digital image captured with white light; and

[0021] FIG. 8 is a photographic reproduction of the same ROI as shown in FIG. 7, in this case illuminated with narrowband LEDs of a particular wavelength associated with an absorbance peak of the biomolecule(s) present in the ROI.

DETAILED DESCRIPTION

[0022] As mentioned above, clear, high-contrast images of selected specimens are vital for diagnostic impressions, particularly when performing pre-cancer and cancer screening. In accordance with the principles of the present invention, it is proposed to use narrowband light sources, operating at specific pre-determined wavelengths, to produce extremely high contrast images of the portion of the anatomy under study (that is, the “region of interest” or ROI).

[0023] FIG. 1 illustrates exemplary types of medical instrumentation that are used to perform optical imaging and include an illumination source that may be formed to include the LED-based system of the present invention. In particular, FIG. 1 depicts a side view of an exemplary colposcope 1, used in the examination of the cervix (e.g., to study the vasculature system of the cervix). While the specific instrument shown in FIG. 1 is rather compact (and thus portable), many colposcopy systems are large combinations of elements situated in an examination room. An exemplary dermatoscope 2 is also shown in FIG. 1. This type of medical instrumentation is used to view the skin (often with some type of oil or lotion applied to the surface of the skin before bringing the dermatoscope in contact.

[0024] Medical instrumentation such as that shown in FIG. 1 is typically based upon the use of a “white light” (full visible spectrum) source to aid the medical professional performing the examination to clearly see the “region of interest” (referred to hereinafter as the “ROI”). It has been known for years, however, that light at certain wavelengths can assist in improving the visualization of blood vessels, skin pigments, mucous, and the like. For example, imaging the cervix with “green” or “blue” light has been found to produce higher contrast images of the underlying vasculature than illumination with white light, since the absorbance spectrum of hemoglobin (a major component of the vessels) includes peaks in the visible part of the spectrum at wavelengths of about 415 nm (“blue” filtered light) and about 540 nm (“green” filtered light). Similar green/blue filters are also used in the study of oral mucosa and submucosa for the presence of premalignant lesions. Abnormal lesions or melanocytes on the surface of the skin (or in the tissue layers immediately beneath the surface) may be better distinguished by using “red” filtered light (a wavelength of about 625 nm) or “yellow” filtered light (a wavelength of about 580 nm).

[0025] In the prior art, the medical imaging apparatus utilized various “color” filters in combination with the standard white light source to alter the color of the ROI. As mentioned above, since there is no standard wavelength or spectral bandwidth defined for these filters, different clinical settings may apply “green” filters (using “green” as just one example) that transmit so-called green light at different wavelengths, perhaps with different bandwidths. Moreover, many of these filters may be wideband devices (e.g., bandwidths over 50 nm) that are too broad in spectral response to create an image that clearly delineates boundaries between normal and abnormal tissue. As a result, the use of such filters may produce less effective images in some cases, or lead to less consensus between different images of differing qualities. Additionally, the utilization of these filters in combination with a white light source inevitably diminishes the intensity of the transmitted beam, and captured images often appear darker than they should.

[0026] In accordance with the principles of the present invention, it is proposed to eliminate the use of such color-based filters and, instead, provide an illumination source comprised of individual light emitting diodes (LEDs) specifically formed to operate at the wavelengths of interest (e.g., “green”, “blue”, “red”, “yellow”, etc.). Advantageously, LEDs may be configured to generate a high intensity, narrowband beam that is well-suited for these medical imaging purposes where the ability to provide a proper diagnosis relies on the ability to create a high contrast image for review by the medical professionals.

[0027] When used as an illumination source for a colposcope, the inventive LED-based source utilizes one or more LEDs that emit at specifically-defined wavelengths that are referenced as “green” and “blue”. The green and blue wavelengths emitted by the LEDs is absorbed by the vessels, while being reflected by the surrounding tissue that lacks hemoglobin. This increases the contrast with which vessels appear in the image. The narrower the bandwidth of the blue and green light (i.e., bandwidths on the order of about 30 nm, or perhaps less) around hemoglobin's absorbance peaks, the greater is the contrast of the vessels in the resulting image. The high contrast between the tissues and vessels significantly improves the visualization of blood vessel patterns, where certain patterns are a known indicator of tissue abnormality. Therefore, the ability to create (and thereafter store) digital images with this level of clarity is a vital need for diagnostic impressions of pre-cancer and cancer (for studying oral mucosa and submucosa as well).

[0028] As will also be discussed below, inasmuch as the two different wavelengths penetrate to a different depth within the ROI, by controlling the sequence of illumination for these LEDs (e.g., a “green” exposure, followed by a “blue” exposure), variations in the vasculature at different levels within the tissue may be discerned, providing a “three-dimensional” imaging result.

[0029] When used as an illumination source for a dermatoscope, the wavelengths for “red” and “yellow” light beams are known to coincide with the absorbance peaks of medically-relevant pigments (e.g., melanocytes).

[0030] In accordance with the principles of the present invention, the number of separate LEDs used, as well as their relative placement within the illumination source, provides the ability to individually manipulate the brightness of the narrowband illumination such that high quality, high contrast images are captured with sufficient brightness and clarity.

[0031] In a specific embodiment of the present invention, a scopic diagnostic tool is utilized to illuminate a particular ROI with a collection of illumination sources operating at specific, well-defined wavelengths. In many cases, a first set of LEDs (all operating at a first defined wavelength λ.sub.1) and a second set of LEDs (all operating at a second defined wavelength λ.sub.2) are used as part of the imaging system for these scopes. The LEDs are particularly selected to exhibit a narrow bandwidth to produce a high contrast result, particularly to aid in delineating the boundary between normal and abnormal areas within the ROI. For example, LEDs operating at a “green” wavelength of λ.sub.1≈540 nm that exhibit a full-width-half-maximum (FWHM) of 30 nm, and LEDs operating at a “blue” wavelength of λ.sub.2≈415 nm that exhibit a FWHM of 12 nm can be used, where the FWHM is a well-understood figure of merit defining the distance from a given center wavelength where the output emission drops below half of the maximum emission value. The center wavelength of a given LED is preferably maintained within a narrow range to ensure that images collected using different instruments will be of similar quality.

[0032] FIG. 2 is a simplified isometric view of an exemplary illumination source 10 formed in accordance with the present invention to be utilized within medical instrumentation such as that illustrated in FIG. 1. In this particular configuration, illumination source 10 is formed to include a pair of opposing apertures 12, 14 through which a narrowband beam from the included LEDs is emitted and directed to an ROI. A central aperture 16 includes a photodetecting arrangement that captures the return light from the ROI. For example, the photodetecting arrangement may take the form of a CCD camera or, preferably, a CMOS detector with appropriate filtering to block stray light outside of the LED wavelengths. As will be discussed in detail below, one or more LEDs may be located at each aperture 12 and 14 (with a white light source in most cases co-located with the LEDs). Additional apertures may be disposed at different locations around the periphery of central aperture 16 to allow for multiple sets of LEDs to be used for narrowband imaging in accordance with the principles of the present invention.

[0033] FIG. 3 is a block diagram side view of an exemplary configuration of illumination source 10, in this illustration shown as being used in association with a particular ROI. In this example, a first narrowband LED 32 (operating at a first specifically-defined wavelength λ.sub.1) is positioned in alignment with aperture 12. When illumination source 10 is part of a colposcopic system, first narrowband LED 32 may be a “green” LED, emitting at a center wavelength λ.sub.1≈540 nm, with a FWHM value of 30 nm. When illumination source 10 is part of a dermatoscope, first narrowband LED 32 may be a “red” LED, emitting at a center wavelength λ.sub.1≈625 nm, with a FWHM value of 16 nm. Lensing elements 33 are positioned beyond the output from first LED 32 and used to enable the focusing of the narrowband output from first LED 32 toward the ROI.

[0034] Also shown in FIG. 3 is a second narrowband LED 34, operating at a second specifically-defined wavelength and positioned behind aperture 14 of instrumentation 10. When illumination source 10 is part of a colposcope, second narrowband LED 34 may be a “blue” LED, emitting at a center wavelength μ.sub.2≈415 nm, with a FWHM value of 12 nm. When illumination source 10 is part of a dermatoscope, second narrowband LED 34 may be a “yellow” LED, emitting at a center wavelength μ.sub.2580 nm, with a FWHM value of 22 nm. Lensing elements 35 are positioned beyond the output from second LED 34 and used to enable the focusing of the narrowband output from second LED 34 toward the ROI.

[0035] A conventional white light source 31 is also shown in FIG. 3, where it is to be understood that the inclusion of white light source 31 is optional, but preferable, since in most cases the medical instrumentation would utilize white light source 31 to illuminate the ROI for a portion of an examination and then energize narrowband LEDs 32, 34 as necessary. Indeed, the turning “on” and “off” of LEDs 32 and 34 is typically under the control of the individual performing the examination, allowing for the capture of high contrast images at specific points in time during the examination procedures. As mentioned above, the activation of the narrowband LEDs may be controlled such that the first-wavelength LEDs 32 are energized for a period of time, and then the second-wavelength LEDs 34 are energized for a different period of time, where the separate activation may provide additional imaging clarity of subsurface elements associated with the different depths penetrated by the different wavelengths.

[0036] A photoreceiving element 40 is shown in FIG. 3 as positioned behind central aperture 16, with lensing elements 39 disposed at the entrance of photoreceiving element 40. In accordance with the optical imaging properties of medical instruments, the illumination reflected back towards illumination source 10 from the ROI is captured by photoreceiving element 40 and processed using various types of analysis, well known (and also currently evolving) in the art. Photoreceiving element 40 may comprise, for example, a CCD-based camera or a CMOS detector with appropriate wavelength filtering.

[0037] FIG. 4 is front view of the particular arrangement of LEDs 32 and 34 as shown in FIG. 3. FIG. 5 is a front view of an alternative illumination system 50 utilizing pairs of apertures disposed around central aperture 16. In this particular arrangement a first aperture 52 is positioned at the 0° location around the circular form of illumination system 50, with a second aperture 54 located at the 180° position. A second pair of apertures is disposed orthogonal to apertures 52 and 54, with one aperture 56 located at the 90° position and a remaining aperture 58 located at the 270° position. In this particular configuration, first-wavelength (λ.sub.1) LEDs 32-1 and 32-2 are disposed behind apertures 52 and 54 (respectively), and second-wavelength (λ.sub.2) LEDs 34-1 and 34-2 are disposed behind apertures 56 and 58 (respectively).

[0038] FIG. 6 shows yet a different arrangement. Here, an illumination system 60 maintains the same set of four apertures 52, 54, 56 and 58 as shown in FIG. 5, but in this case is configured to use (λ.sub.1,λ.sub.2) pairs of LEDs at each of the four quadrant locations as defined above with respect to the arrangement of FIG. 5. A first pair is identified as (LED 32.sub.1, LED 34.sub.1); a second pair is identified as (LED 32.sub.2, LED 34.sub.2); a third pair is identified as (LED 32.sub.3, LED 34.sub.3); and a fourth pair is identified as (LED 32.sub.4, LED 34.sub.4).

[0039] In each of these embodiments, a specific switching sequence may be used to control the illumination of the separate LEDs, where as mentioned above it is typically the individual performing the examination who controls when the LEDs are turned “on” and “off”. However, it is to be understood that a computer-based control of LED sequencing may also be implemented in certain applications.

[0040] The ability of the narrowband, wavelength-specific LEDs to provide a higher quality, sharper image of an exemplary ROI is shown by comparing a photographic reproduction of a prior art digital image displayed in FIG. 7 (captured using a traditional white light source) to the digital image displayed in FIG. 8, which was captured using green LEDs as an illumination source in accordance with the teachings of the present invention. The higher contrast result of FIG. 8 is evident in the detailed vasculature of the ROI, particularly in comparative regions A (for example).

[0041] It is to be noted that as mentioned above an exemplary LED-based illumination source formed in accordance with the present invention most likely also includes the standard white light illumination source, as still important to capture various other details of the ROI. In an exemplary procedure, for example, a white light illumination source may be used for most of examination, with the narrowband LED-based illumination source activated (as controlled by the clinician, perhaps) during specific periods of time when the vasculature, skin pigmentation, mucosa, or the like, need to be imaged in detail.

[0042] In general, the descriptions of the details and embodiments of the narrow band illumination system have been presented for purposes of illustration, but are not intended to be exhaustive or limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the describes embodiments. The terminology used herein was chosen to best explain the principles of the embodiments, the practical application or technical improvement over technologies found in the prior art.