MICROORGANISM INSPECTION APPARATUS AND METHOD

Abstract

An inspection apparatus includes a stirring/mixing means for stirring and mixing a sample solution in a batch-type sample container, an excitation light source including a light source that irradiates an irradiation plane of the sample container in the vertical direction, a light-receiving means arranged on the lateral face side of the sample container, and a control means for calculating the number of microorganisms contained in a sample in the sample solution.

Claims

1: A microorganism inspection apparatus for measuring the number of microorganisms in a sample solution, comprising: stirring/mixing means for adding a sample and a fluorescent staining reagent into a batch-type sample container formed of a light-transmitting material, and stirring and mixing a sample solution; an excitation light source including a light source that irradiates an irradiation plane of the sample container with an excitation light beam in a vertical direction while the sample solution is stirred by the stirring/mixing means; light-receiving means arranged on a lateral face side of the sample container, the light-receiving means being configured to detect a fluorescence beam emitted in response to the excitation light beam from the excitation light source; and control means for converting the beam detected by the light-receiving means into an electric signal to detect the number of light emissions, and calculating the number of microorganisms contained in the sample in the sample container from the number of light emissions.

2: The microorganism inspection apparatus according to claim 1, wherein the excitation light source is arranged above or below the sample container.

3: The microorganism inspection apparatus according to claim 1, wherein: a reflective member is provided in the sample container, the excitation light source is arranged on a lateral side of the sample container, and an excitation light beam emitted from the excitation light source irradiates the irradiation plane in the vertical direction by being reflected by the reflective member.

4: The microorganism inspection apparatus according to claim 1, further comprising an optical guide bar between the excitation light source and the irradiation plane.

5: The microorganism inspection apparatus according to claim 1, further comprising a shielding plate on a side opposite to the excitation light source across the irradiation plane.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0030] FIG. 1 is a perspective view illustrating the entire microorganism inspection apparatus according to an embodiment of the present invention.

[0031] FIG. 2 is a diagram illustrating the electrical control configuration of the inspection apparatus according to the present embodiment.

[0032] FIG. 3 is a side view of a measurement unit according to the present embodiment.

[0033] FIG. 4 is a view illustrating exemplary shapes of a diaphragm.

[0034] FIG. 5 is a chart illustrating a measurement flow according to the present embodiment.

[0035] FIG. 6 is a side view of a measurement unit according to another embodiment of the present invention.

[0036] FIG. 7 is a side view of a measurement unit according to further another embodiment of the present invention.

[0037] FIG. 8 is a side view of a measurement unit according to still another embodiment of the present invention.

[0038] FIG. 9 is a side view of a measurement unit according to yet another embodiment of the present invention.

[0039] FIG. 10 is a side view of a measurement unit according to yet another embodiment of the present invention.

[0040] FIG. 11 is a side view of a measurement unit according to yet another embodiment of the present invention.

[0041] FIG. 12 is a schematic planar cross-sectional view of a measurement unit of conventional art.

[0042] FIG. 13A is a schematic cross-sectional view illustrating a flat plate with a predetermined thickness having a thread cutting hole formed therein as an embodiment of a means for converting a light beam into a collimated light beam.

[0043] FIG. 13B is a schematic cross-sectional view illustrating an LED light source with a front face provided with a convex lens as an embodiment of a means for converting a light beam into a collimated light beam.

[0044] FIG. 14 is a view illustrating a detection method performed with a conventional inspection apparatus.

DESCRIPTION OF EMBODIMENTS

[0045] To describe the characteristics of the invention of the present application, a summary of conventional art described in Patent Literature 3 will be described first. FIG. 12 is a schematic planar cross-sectional view of a measurement unit of the conventional art.

[0046] An inspection apparatus of the conventional art includes a batch-type sample container 105 formed of a light-transmitting, transparent material (for example, glass, quartz, or acrylic resin), and a measurement unit 106 that optically counts the number of microorganisms in the sample container 105. The sample container 105 includes a rotor 107 to stir a sample solution S in the sample container 105. The rotor 107 is configured to be rotationally driven by a stirrer provided in a body (not illustrated).

[0047] The inspection apparatus illustrated in FIG. 12 is formed such that it has a width of 300 mm, a depth of 300 mm, a height of 100 mm, and a weight in the range of about 2 to 4 kg. Therefore, the inspection apparatus can be carried around while being put in a handheld trunk or a backpack (not illustrated), for example, and thus can perform measurement on a vessel or outside.

[0048] In addition, the batch-type sample container 105 formed of a light-transmitting, transparent material is formed in the shape of a prism having a bottom face with a size of 50 mm×50 mm and a height of 60 mm, and has an internal volume set to 100 ml (milliliter) when the water level in the container is 40 mm. The shape of the sample container 105 is not limited to such a prism shape, and may be a cylindrical shape or a cubic shape as long as an internal volume of about 100 ml (milliliter) can be secured.

[0049] The measurement unit 106 includes a sample container housing unit 109 that houses and holds the sample container 105, a light source unit 113 that emits an excitation light beam toward the sample container 105, and a light-receiving unit 119 for observing microorganisms stained by a luminescence reagent and floating in the sample container 105 with the excitation light beam emitted from the light source unit 113. The light-receiving unit 119 is electrically connected to a CPU (not illustrated) that counts the number of microorganisms in the sample solution S and performs an information processing operation or a statistical processing operation on the measurement results, for example.

[0050] The sample container housing unit 109 is formed with holding plates 108a and 108b surrounding at least two faces of the sample container 105, and houses and holds the sample container 105 so as not to block a light beam emitted from the light source unit 113.

[0051] In addition, as illustrated in FIG. 12, the light source unit 113 is arranged so as to allow an excitation light beam to become incident on an irradiation plane G of the sample container 105 in the direction of the normal P to the irradiation plane G. The light source unit 113 includes an LED light source 110 arranged in the vicinity of the sample container housing unit 109, a means 111 for converting a light beam into a collimated light beam, arranged on the front face side of the LED light source 110 and adapted to convert a diffused light beam into a collimated light beam, and an excitation filter 112 that irradiates the sample container 105 with an excitation light beam of a slit-collimated light beam.

[0052] FIG. 13 are schematic cross-sectional views each illustrating an embodiment of the means 111 for converting a light beam into a collimated light beam. In the example illustrated in FIG. 13A, a flat plate 131 with a predetermined thickness, which has a thread cutting hole 132 with a predetermined diameter drilled therein, is formed as the means 111 for converting a light beam into a collimated light beam, and the thickness L of the flat plate 131 and the diameter of the thread cutting hole are appropriately set in accordance with the optical path length. This allows a scattered light beam with an incidence angle θ emitted from the LED light source 110 to be converted into a collimated light beam while passing through the thread cutting hole 132. In the example illustrated in FIG. 13A, the optimum conditions for θ and L are determined through a test of the SN ratio, and for example, provided that the thread cutting hole 132 has a size of M3 (the outside diameter of the threaded hole)×0.5 (pitch), the optimum conditions are 0 of 9.5° and L of 15 mm.

[0053] The means 111 for converting a light beam into a collimated light beam illustrated in FIG. 13B is the LED light source 110 with a front face provided with a convex lens 133. A scattered light beam emitted from the LED light source 110 is converted into a collimated light beam when output to the outside through the convex lens 133.

[0054] The light-receiving unit 119 is provided such that its light-receiving surface F is arranged at an angle perpendicular to an excitation light beam in the direction of the normal P from the light source unit 113. The light-receiving unit 119 includes a detector 114 arranged and configured to receive fluorescence along an optical axis perpendicular to an excitation light beam emitted from the LED light source 110 toward the sample container 105, a light-receiving filter 115 arranged on the front face side of the detector 114, a condensing lens 116 arranged on the front face side of the light-receiving filter 115, a slit 117 arranged on the front face side of the condensing lens 116, and a relay lens 118 arranged in the gap between the slit 117 and the sample container 105 and adapted to excite a fluorescent material contained in microorganisms and collect the resulting fluorescence emission to form an image.

[0055] In such an inspection apparatus, since the rotor 107 rotates on the bottom face of the sample container 105 as illustrated in FIG. 12, the sample solution S basically rotates in the direction along the plane of the bottom face. Usually, the measurement range is set vertically long in the vertical direction so as to allow for efficient detection of the number of microorganisms based on the direction of rotation of the sample solution S.

[0056] FIG. 14 is a view illustrating a detection method performed with the conventional inspection apparatus. A measurement range 140 is set vertically long to specifically allow for the detection of fluorescence emitted from organisms and passing through a region with a height of 10 mm and a width of 1 mm. Due to such a configuration, LED light sources 110 are arranged at three points in the conventional inspection apparatus to cover the entire measurement range 140 with a height of 10 mm. To detect a light beam from an intended observation point among light beams from the three points, the light-receiving unit includes optical components, such as the condensing lens 116, the slit 117, and the relay lens 118. Therefore, in the conventional art, it is necessary to dispose the light source unit 113 and a number of optical components as the light-receiving unit 119 around the sample container 105, which may result in an increased overall size of the inspection apparatus.

[0057] In view of the foregoing, an embodiment of the present invention will be described hereinafter. FIG. 1 is a perspective view illustrating the entire microorganism inspection apparatus according to an embodiment of the present invention.

[0058] An inspection apparatus 1 of the present embodiment includes a body unit 2 that incorporates a control mechanism, such as a CPU board, and performs an operation of processing measurement results, for example, an operation unit 3 that is arranged next to the body unit 2 and includes operation buttons, for example, and a display unit 4 for displaying the measurement results, for example. The display unit 4 includes a liquid crystal panel, for example. The operation unit 3 includes a power button 3a, a measurement start button 3b, an external output button 3c, and a setting button 3d. Pressing the power button 3a can control the on/off switching, and pressing the measurement start button 3b can start measurement. In addition, pressing the external output button 3c can transfer data to an external printer or personal computer, and pressing the setting button 3d can switch the type of measurement, such as the setting of the size of microorganisms as a measurement target, change the setting of a threshold, and change the setting of the measurement time.

[0059] The body unit 2 includes a measurement unit 6. The measurement unit 6 houses a batch-type sample container 5 and optically counts the number of microorganisms in a sample solution S in the sample container 5. The sample container 5 is formed of a transparent material, such as glass, quartz, or acrylic resin, for example, and thus is configured to transmit light. The sample container 5 houses a rotor 7, and the rotor 7 stirs the sample solution S. The rotor 7 is housed in the sample container 5 together with the sample solution S and a luminescence reagent, and is closed with a lid 30. In addition, the rotor 7 is configured to be, when the sample container 5 is housed in the measurement unit 6, rotationally driven by a stirrer 27 incorporated in the measurement unit 6.

[0060] The inspection apparatus 1 illustrated in FIG. 1 is dimensioned such that it can be carried around while being put in a handheld trunk, for example. Therefore, it is possible to perform measurement on a vessel or outside by carrying around the inspection apparatus.

[0061] The sample container 5 is formed in the shape of a prism having a bottom face with a size of 50.5 mm×51.5 mm and a height of 67 mm, and has a volume set to about 100 ml when the water level in the container is 43 mm. Although the sample container 5 is formed in the shape of a prism in the present embodiment, the sample container 5 may have other shapes, such as a cylindrical shape. In such a case, however, the sample container 5 is desirably dimensioned to be able to secure a volume of about 100 ml to facilitate measurement.

[0062] Further, the electrical control configuration of the inspection apparatus 1 of the present embodiment will be described based on FIG. 2. A CPU board 23, which is supplied with power from an AC power supply 21 or a secondary battery 22, is arranged in the center of a housing 20 forming the body unit 2. The CPU board 23 analyzes an output signal obtained by converting a light beam into electricity with a detector 14, determines if the luminance is greater than or equal to a given luminance level, counts pulses of a given luminance signal, and controls on/off of an LED light source 10, for example. An AC/DC converter 24 is arranged between the AC power supply 21 and the CPU board 23.

[0063] The CPU board 23 has electrically connected thereto the detector 14, the LED light source 10, a RAM 25 as a read/write memory portion, and a ROM 26 as a read-only memory portion.

[0064] Besides, the CPU board 23 has connected thereto the stirrer 27, which rotates the rotor 7 with a magnetic force, the display unit 4 formed with a liquid crystal panel, for example, a cooling fan 28 for control devices, such as the CPU board 23, and an external output terminal 29, such as RS-232C.

[0065] Next, the configuration of the measurement unit 6 of the present embodiment will be described based on FIG. 3. FIG. 3 is a side view of the measurement unit 6 of the present embodiment. The sample solution S and the rotor 7 are put in the sample container 5, and the rotor 7 can be rotated by the stirrer 27 provided in the measurement unit 6. A light source unit 13 is provided on the bottom face side of the sample container 5, and an excitation filter 12 is provided on the front face side of the LED light source 10, and further, a diaphragm 11 is provided on the front face side of the excitation filter 12. As the excitation filter 12, a bandpass filter can be used. The shape of the diaphragm will be described later. The light-receiving unit 19 is provided on the lateral face side of the sample container 5, and includes the detector 14 and a light-receiving filter 15. As the detector 14, it is possible to use a photomultiplier, for example, that is arranged and configured to receive fluorescence emitted by microorganisms that have been irradiated with a light beam emitted from the light source unit 13 toward the sample solution S in the sample container 5. As the color of the LED light source 10, blue is preferably used.

[0066] The rotor 7 is arranged on the bottom face of the sample container 5. Thus, when the rotor 7 is rotated by the stirrer 27, the flow of water is stirred mainly in the horizontal direction along the bottom face. Therefore, although excitation light beam irradiation is performed in a direction parallel with the flow of water in the conventional art, in the present embodiment, excitation light beam irradiation is performed in a direction perpendicular to the flow of water. Therefore, the influence of the flow of water is reduced, and detection of the number of microorganisms can be performed with higher accuracy and with suppressed irradiation unevenness.

[0067] FIG. 4 illustrates exemplary shapes of the diaphragm 11. A diaphragm A has a circular shape, a diaphragm B has a shape with arc-like portions, such as an elliptical shape, an elongated circular shape, or a rounded rectangular shape, a diaphragm C has a square shape, and a diaphragm D has a rectangular shape. Such diaphragms are selectively used according to the intended use.

[0068] An excitation light beam excited by the LED light source 10 passes through the excitation filter 12 so that unwanted wavelengths are filtered out, and then, the beam shape is adjusted by the diaphragm 11. Accordingly, it is possible to reduce variation in the amount of fluorescence emitted by microorganisms depending on the portion through which an excitation light beam passes.

[0069] In the present embodiment, the number of optical members on the side of the light source unit 13 and the side of the light-receiving unit 19 can be reduced in comparison with that of the conventional art, and thus, the optical configuration can be simplified.

[0070] Specifically, the number of the LED light sources 10 can be reduced from 6 to 1, and the number of the excitation filters 12 can be reduced from 2 to 1. The diameter of the LED light source 10 can also be reduced to half, specifically, from 25 mm to 12.5 mm. In addition, the number of pieces of cover glass provided on the excitation filter 12 can also be reduced from 2 to 1, and the size of the cover glass can also be reduced.

[0071] Regarding the light source unit 13, although the LED light source 10 is used as a light source, it is possible to use not only the LED light source 10 but also a collimated-light-beam LED light source capable of emitting a collimated light beam, a laser light source, or a light bulb as long as a fluorescent material contained in microorganisms can be excited.

[0072] Regarding the optical components on the side of the light-receiving unit 19, there is no need to use a relay lens, a slit, or a cylindrical lens provided in the conventional art. In addition, regarding the light-receiving filter 15 and the detector 14, a single light-receiving filter 15 and a single detector 14 may be provided.

[0073] In this manner, reducing the number of optical components on the side of the light source unit 13 and the side of the light-receiving unit 19 to simplify the configuration can reduce the overall size of the apparatus.

[0074] Although the sample container 5 in the present embodiment is formed in the shape of a prism, the sample container 5 may be formed in a cylindrical shape. Further, the sample container 5 may also be formed in the shape of a polygonal prism, such as a triangular prism, a pentagonal prism, or a hexagonal prism.

[0075] Although an example in which a photomultiplier (PMT) is used as a light-receiving sensor of the light-receiving unit 19 has been illustrated, the present invention is not limited thereto, and it is possible to use various photodetectors, such as a silicon photodiode (SiPD) and an avalanche photodiode (APD), that can detect light emission of a fluorescent material contained in microorganisms as with a photomultiplier (PMT).

[0076] FIG. 5 is a flowchart illustrating a measurement flow. A measurement flow of each step will be described based on FIG. 5.

[0077] (Step 1) An operator collects 100 ml (milliliter) of a sample from ballast water at a temperature of about 20° C., using a pipette, for example, and then puts the sample into the sample container 5.

[0078] (Step 2) A fluorescent staining reagent is added into the sample container 5. As the fluorescent staining reagent, it is possible to use commonly known calcein AM (Calcein-AM manufactured by Promocell GMBH in Germany) or FDA (Fluorescein Diacetate manufactured by Tokyo Chemical Industry Co., Ltd.), for example. Calcein AM tends to stain phytoplankton more easily, while FDA tends to stain zooplancton more easily. Therefore, when staining with a staining reagent is performed with a reagent containing a mixture of calcein AM and FDA, it is possible to shorten the reagent staining time and specifically reduce the time required for staining to half that of the conventional art.

[0079] (Step 3) The operator puts the rotor 7 into the sample container 5 and places the sample container 5 in the measurement unit 6 of the inspection apparatus 1, and then puts the lid 30 of the measurement unit 6, thereby completing the preparation for measurement. Then, the operator presses the power button 3a to cause the rotor 7 to rotate with the drive of the magnetic stirrer 27 incorporated in the measurement unit 6, and thus stir the sample solution S.

[0080] (Step 4) The operator presses the measurement start button 3b of the operation unit. Then, after a predetermined time has elapsed, the LED light source 10 is lighted so that the sample container 5 is irradiated with a light beam transmitted through the bandpass filter 12 for an excitation light beam. At this time, for example, the sample container 5 is irradiated with a light beam with a wavelength of 450 nm to 490 nm as the wavelength characteristics so that a specimen (i.e., microorganisms) in the sample container 5 emits fluorescence.

[0081] (Step 5) The detector 14 of the light-receiving unit 19 detects the fluorescence.

[0082] (Step 6) The detector 14 detects the emission of fluorescence by converting the light energy into electrical energy utilizing the photoelectric effect, and also with a current amplification function added thereto. The detected electric signal is sent to the CPU board 23 so that received-light waveforms that are greater than or equal to a given threshold are counted.

[0083] (Step 7) Further, the CPU board 23 estimates the number of microorganisms existing in 100 ml (milliliter) of water in the sample container 5 from the counted value of the received-light waveforms, and then displays on the display unit 4 information about whether the estimated number of microorganisms satisfies the effluent standard.

[0084] The configuration of the measurement unit 6 according to another embodiment will be described based on FIG. 6. The present embodiment differs from the previous embodiment in that the light source unit 13 is arranged above the sample container 5. When light beam irradiation is performed from above as in the present embodiment, the light beam may not travel straight and may not reach the inside of the water due to ruffle generated at the boundary portion between the air and the water surface or due to the difference in refractive index between the air and the water if no measure is taken. Thus, an optical guide bar 50 is provided ahead of the diaphragm 11 of the light source unit 13, and the optical guide bar 50 is arranged in a range including the air and the water. In the present embodiment, the lid 30 is preferably provided with a hole for passing the optical guide bar 50. A light beam emitted from the LED light source 10 and transmitted through the excitation filter 12 and the diaphragm 11 passes through the optical guide bar 50, and thus is guided to the vicinity of the measurement unit. The optical guide bar 50 is formed of transparent resin, such as acrylic resin, and guides a light beam utilizing internal reflection. Accordingly, the influence between the air and the water can be reduced, and a light beam from the light source unit 13 can be guided to the vicinity of the measurement unit for microorganisms. The other configurations of the light source unit 13 and the light-receiving unit 19 are similar to those of the previous embodiment. Thus, the description thereof is omitted herein.

[0085] The configuration of the measurement unit 6 according to further another embodiment will be described based on FIG. 7. The present embodiment differs from the previous embodiments in that the light source unit 13 is arranged on the same side as the light-receiving unit 19 that is arranged on the lateral side of the sample container 5. In the present embodiment, a mirror 51 is arranged in the sample solution S to allow microorganisms to be irradiated with a light beam from below. Specifically, the direction of a light beam from the light source unit 13 is switched to the vertical direction so that the light beam irradiates the microorganisms. The light-receiving unit 19 is arranged so as to receive a light beam which the microorganisms emit as fluorescence, which has become incident on the microorganisms in the vertical direction, at an angle perpendicular thereto. The other configurations of the light source unit 13 and the light-receiving unit 19 are similar to those of the previous embodiments. Thus, the description thereof is omitted herein. Although the mirror 51 is used to switch the direction of a light beam in the present embodiment, it is also possible to use other elements, such as a prism 52, to switch the direction of a light beam. In the present embodiment, since the light source unit 13 and the light-receiving unit 19 are arranged on the same lateral side, the size of the inspection apparatus can be reduced.

[0086] The configuration of the measurement unit 6 according to still another embodiment will be described based on FIG. 8. In the present embodiment, the light source unit 13 is arranged on the same side as the light-receiving unit 19 that is arranged on the lateral side of the sample container 5 as in the previous embodiment. In the present embodiment, the light source unit 13 is arranged at a position above the light-receiving unit 19, and a mirror 51 is arranged in the sample solution S so as to allow microorganisms to be irradiated with a light beam from above. Specifically, the direction of a light beam from the light source unit 13 is switched to the vertical direction so that the light beam irradiates the microorganisms. The light-receiving unit 19 is arranged so as to receive a light beam which the microorganisms emit as fluorescence, which has become incident on the microorganisms in the vertical direction, at an angle perpendicular thereto. The other configurations of the light source unit 13 and the light-receiving unit 19 are similar to those of the previous embodiments. Thus, the description thereof is omitted herein. Although the mirror 51 is used to switch the direction of a light beam in the present embodiment, it is also possible to use other elements, such as a prism 52, to switch the direction of a light beam. In the present embodiment, the light source unit 13 and the light-receiving unit 19 are also arranged on the same lateral side. Thus, the size of the inspection apparatus can be reduced.

[0087] FIG. 9 illustrates the configuration of the measurement unit 6 according to yet another embodiment. The present embodiment differs from the other embodiments in the configuration of the light-receiving unit 19. The light-receiving unit 19 according to the present embodiment is provided on the lateral face side of the sample container 5, and includes the detector 14 and the light-receiving filter 15. A condensing lens 16 is arranged on the front face side of the light-receiving filter 15, and a slit 17 is arranged on the front face side of the lens 16. In addition, a relay lens 18 is arranged between the slit 17 and the sample container 5. The relay lens 18 has functions of exciting a fluorescent material contained in microorganisms, and collecting the resulting fluorescence emission to form an image.

[0088] The slit 17 is used to narrow the observation plane in a slit form. Using the slit 17 can narrow the light-receiving area of the light-receiving surface and can also narrow the area of the background fluorescence emission that would become noise. This improves the ratio of a signal of the fluorescence emission of microorganisms to the background fluorescence emission, and thus improves detection accuracy for the fluorescence emission of microorganisms.

[0089] The configuration of the measurement unit 6 according to yet another embodiment will be described based on FIG. 10. In the present embodiment, the light source unit 13 is arranged below the sample container 5 so as to emit an excitation light beam vertically upward, but the present embodiment differs from the previous embodiment in that a light-shielding plate 55 is arranged in the vicinity of the water surface. Such a configuration allows a light beam, which has irradiated microorganisms and passed therethrough in the upward direction, to be shielded by the light-shielding plate 55. Thus, even when the lid 30 is not provided, it is possible to perform measurement without the operator feeling dazzling. The other configurations of the light source unit 13 and the light-receiving unit 19 are similar to those of the previous embodiments. Thus, the description thereof is omitted herein.

[0090] The configuration of the measurement unit 6 according to yet another embodiment will be described based on FIG. 11. The present embodiment differs from the previous embodiment in that the optical guide bar 50 is provided for excitation light beam irradiation in addition to the light-shielding plate 55 provided as in the previous embodiment so that an excitation light beam can be reliably transmitted from the bottom face of the sample container 5 to the vicinity of the measurement range of microorganisms. Such a configuration allows a light beam, which has irradiated the microorganisms and passed therethrough in the upward direction, to be shielded by the light-shielding plate 55. Thus, even when the lid 30 is not provided, it is possible to perform measurement without the operator feeling dazzling. Further, the measurement range of microorganisms, including its vicinity, can be reliably irradiated with an excitation light beam. The other configurations of the light source unit 13 and the light-receiving unit 19 are similar to those of the previous embodiments. Thus, the description thereof is omitted herein.

[0091] Although the aforementioned embodiments have illustrated examples in which a single LED light source is provided, it is also possible to arrange a plurality of LED light sources, such as three LED light sources. In such a case, if the LED light sources are arranged on the bottom face side of the sample container 5, for example, the LED light sources are preferably arranged in the horizontal direction along the direction of the flow of the sample solution S based on the rotor 7. In addition, in such embodiments, although the rotor 7 is arranged on the bottom face and excitation light beam irradiation is performed in the vertical direction with respect to the rotor 7, it is also possible to dispose the rotor 7 on the lateral face and perform excitation light beam irradiation in the horizontal direction perpendicular to the rotor 7.

REFERENCE SIGNS LIST

[0092] 1 Inspection apparatus [0093] 2 Body unit [0094] 3 Operation unit [0095] 4 Display unit [0096] 5 Sample container [0097] 6 Measurement unit [0098] 7 Rotor [0099] 8 Holding plate [0100] 9 Sample container housing unit [0101] 10 LED light source [0102] 11 Diaphragm [0103] 12 Excitation filter [0104] 13 Light source unit [0105] 14 Detector [0106] 15 Light-receiving filter [0107] 16 Condensing lens [0108] 17 Slit [0109] 18 Relay lens [0110] 19 Light-receiving unit [0111] 20 Housing [0112] 21 AC power supply [0113] 22 Secondary battery [0114] 23 CPU board [0115] 24 AC/DC converter [0116] 25 RAM [0117] 26 ROM [0118] 27 Stirrer [0119] 28 Fan [0120] 29 External output terminal [0121] 30 Lid [0122] 50 Optical guide bar [0123] 51 Mirror [0124] 52 Prism [0125] 55 Light-shielding plate