SINGLE CELL GEL ELECTROPHORESIS FLOW CYTOMETRY

Abstract

A system ensuring the scattering of cells one by one, at a certain speed and direction, on the agarose layer on the lame in SCGE assays is provided. The system includes an electro-magnetic focusing system, a droplet nozzle moving with electro-magnetic guidance, an electric winding creating electromagnetic field for an electro-magnetic charging, an electric motor providing the movement of the lame, an electro-magnetic focusing system, and a control system controlling the electric motor.

Claims

1. A system ensuring a scattering of cells on an LMA in a desired depth and pattern, comprising; an electro-magnetic focusing system, wherein the electro-magnetic focusing system guides the cells in the LMA to be sprayed on a lame constantly moving at a low speed with at least one electric motor and containing a flexible connection determining a scattering pattern of the cells coming out of at least one droplet nozzle, and the electro-magnetic focusing system allows the cells to send individual droplets from the at least one droplet nozzle in different directions in x and y coordinates at the desired depth and a desired speed depending on a nozzle movement with an electro-magnetic direction, the at least one droplet nozzle with the flexible connection, wherein a magnetic material is provided on the at least one droplet nozzles to be used in a movement of the at least one droplet nozzle and enables the individual droplets to exit and move in x and y axes, and the at least one droplet nozzle moves with an electro-magnetic guidance, an electrical winding, wherein the electrical winding provides the electro-magnetic guidance on the movement of the at least one droplet nozzle by determining different current directions and current amounts for an electro-magnetic focusing, the at least one electric motor, wherein the at least one electric motor enables a movement of the lame carrying the LMA, and provides an arrangement of the scattering pattern of the cells by determining a direction and a speed of the movement of the lame with a worm screw and the at least one electric motor, a control system, wherein the control system provides an adjustment of a speed of the electro-magnetic focusing system in accordance with a user's desire, and controls voltage and current directions of electro-magnetic focusing windings and the at least one electric motor in terms of the speed and the direction of the movement of the lame carrying the LMA.

2. The system according to claim 1, comprising the control system enabling a control of the at least one electric motor to move the worm screw in both directions with steps.

3. A device allowing the cells to be scattered on the LMA at the desired depth and pattern, comprising the system according to claim 1.

4. The system according to claim 1, comprising the flexible connection enabling the arrangement of the scattering pattern of the cells in the x and y coordinates.

5. The system according to claim 1, wherein the at least one electric motor is a step motor.

6. The system according to claim 1, wherein the scattering of the cells and the desired depth of the cells in the lame depend on a speed of the at least one electric motor, the movement of the at least one droplet nozzle due to the electro-magnetic focusing, a spray speed at the at least one droplet nozzle, a current amount and current loads of the electrical winding providing the electro-magnetic focusing.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0011] For a better understanding of the invention in question, FIGS. 1-5 and explanations about the drawings are given below.

[0012] FIG. 1 Electro-magnetic focusing system

[0013] FIG. 2 Droplet nozzle moving with electro-magnetic guidance

[0014] FIG. 3 Winding for electromagnetic charging

[0015] FIG. 4 Electric motor system providing the movement of the lame

[0016] FIG. 5 Basic design of the device

REFERENCE NUMBERS

[0017] For a better understanding of the present invention, the elements and their reference numbers are given below. [0018] 1 Electro-magnetic focusing system [0019] 2 Droplet nozzles [0020] 3 Electric winding [0021] 4 Electric motors [0022] 5 Basic device [0023] 6 Magnetic material [0024] 7 Possible directions of movement of the nozzle [0025] 8 Flexible connection [0026] 9 Nozzle tip [0027] 10 Droplet [0028] 11 Copper winding [0029] 12 LMA Lame [0030] 13 Foot [0031] 14 Worm screw [0032] 15 Control system

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0033] In this detailed explanation, the innovation that is the subject of the invention is only explained for a better understanding of the subject with examples that will not have any limiting effect.

[0034] The present invention relates to single cell gel electrophoresis flow cytometry. The invention includes (1) Electro-magnetic focusing system, (1) droplet nozzle moving with electro-magnetic guidance (2), electric winding that creates electromagnetic field for electro-magnetic charging (3), electric motor providing the movement of the lame (4) electro-magnetic focusing system and control system (15) that controls the electric motor.

[0035] The present invention guides the cells in the LMA at the appropriate temperature in the electric field with electro-magnetic focusing and sprays them onto the lame that is constantly moving at low speed with an electric motor. The scattering of the cells and their depth in the lame differ depending on the speed of the electric motor, the movement of the moving nozzle due to electro-magnetic focusing, the spray speed in the nozzle, the amount of current sent and current loads of the electric windings that provide electro-magnetic focusing, and in this way, regular and repeatable cell scattering in the desired pattern, frequency and depth are provided.

[0036] Based on the detailed explanations above, the present invention is a system that allows cells to scatter on the LMA at the appropriate depth and pattern, including; [0037] An electro-magnetic focusing system (1) that guides the cells in the LMA to be sprayed on the lame which constantly moves at low speed with an electric motor and contains a flexible connection (8) that determines the scattering pattern of the cells coming out of the nozzle, allows the cells to send individual droplets from the nozzle in different directions in x and y coordinates, at the desired depth and at the desired speed, depending on the nozzle movement with electro-magnetic direction, [0038] At least one droplet nozzle (2) with a flexible connection (8) that has magnetic material (6) on it to be used in its movement and enables the droplets to exit and move in x and y axes, and that moves with electro-magnetic guidance, [0039] The electrical winding (3), which provides the electro-magnetic guidance movement of the droplet nozzle (2), and which provides electro-magnetic guidance by determining the different current direction and current amount for electro-magnetic focusing, [0040] At least one electric motor (4), which enables the movement of the lame carrying the LMA, and provides the arrangement of the cell scattering pattern by determining the direction and speed of movement of the lame with the worm screw and electric motor, and [0041] The control system (5) which provides the adjustment of the speed of the electro-magnetic focusing (1) in accordance with the user's desire, and controls the voltage and current directions of the electro-magnetic focusing windings and the electric motor (4) (in terms of movement speed and direction of movement) that moves the lame carrying the LMA.

[0042] Other embodiments of the invention are; [0043] A device that allows the cells to be scattered on the LMA at the appropriate (desired) depth and pattern, including the above-mentioned system. [0044] It includes the flexible connection that ensures the arrangement of the cell scattering in the x and y coordinates. [0045] It includes the control system (5) that enables the aforementioned electric motor (4) to be precisely controlled along the length of the lame, in both directions, with very small steps with the worm screw. [0046] Said electric motor (4) is a step motor. [0047] Said scattering of cells and their depth in the lame depend on the speed of the electric motor, the movement of the moving nozzle due to electro-magnetic focusing, the spray speed at the nozzle, the amount of current sent and the current loads of the electric windings that provide electro-magnetic focusing.