Device for electrical measurement of target chemical substance, and method therefor

Abstract

A device for electrical measurement of a target chemical substance includes: an electrode array element including a base, an electrode array section in which electrodes which are disposed on the base and to each of which a test substance is fixed or can be fixed are arrayed, and a terminal array section in which terminals disposed on the base so as to correspond to the electrodes and electrically connected to the electrodes are arrayed; a processing head having an electrode array element support section supporting the electrode array element detachably to make electrical connection to the terminals possible; a container group including a liquid storing section and a measurement container; a support section moving mechanism; and a measurement section capable of measuring a signal generated by applying a predetermined voltage to a test substance fixed to each of the electrodes in the measurement container.

Claims

1. A device for electrical measurement of a target chemical substance, comprising: one or more electrode array elements each including a base, an electrode array section in which one or more electrodes are disposed on the base and to each of which a test substance is fixed, the test substance having a bonding property to a target chemical substance, and a terminal array section in which one or more terminals disposed on the base so as to correspond to the electrodes and electrically connected to the electrodes are arrayed; a processing head including one or more electrode array element support sections supporting the electrode array elements detachably to make electrical connection to the terminals possible; a container group including one or more liquid storing sections for of storing liquid, and one or more measurement containers for receiving the target chemical substance and for receiving the electrode array section of an electrode array element; a support section moving mechanism for relatively moving the one or more electrode array element support sections with respect to the container group such that the test substance fixed to one or more electrodes of the electrodes array section encounters the target chemical substance; and a measurement section capable of measuring a signal generated by applying a predetermined voltage to the test substance fixed to the one or more electrodes of the electrode array section when the electrode array section is received in the measurement containers, wherein the container group includes one or more electrode array element storing sections for of storing the electrode array elements such that the electrode array elements are supported by the electrode array element support sections due to the support section moving mechanism.

2. The device for electrical measurement of a target chemical substance according to claim 1, wherein the base is formed in a plate shape having a predetermined height, a predetermined width, and a predetermined thickness shorter than the predetermined height and the predetermined width, the electrode array element support section includes a plug detachably attached to the terminal array section formed on an upper side of the base and electrically connectable to the terminals of the terminal array section, and the container group includes a liquid storing section or a measurement container having a longer inner width than the predetermined width of the base and an inner depth longer than the predetermined thickness but shorter than the predetermined width.

3. The device for electrical measurement of a target chemical substance according to claim 1, wherein the processing head further includes a suction and discharge mechanism for sucking and discharging gas, and the electrode array element support section includes a dispensing element support section detachably supporting a dispensing element capable of sucking and discharging liquid through a mouth portion at a lower end due to the suction and discharge mechanism, and a plug detachably attached to the terminal array section of the electrode array element and electrically connected to the terminals.

4. The device for electrical measurement of a target chemical substance according to claim 3, wherein the base of the electrode array element includes the dispensing element, the electrode array section is disposed inside the dispensing element, and the terminal array section is disposed inside the dispensing element so as to be located above the electrode array section or is disposed outside the dispensing element.

5. The device for electrical measurement of a target chemical substance according to claim 3, wherein the dispensing element is a dispensing tip, the dispensing element support section includes a nozzle communicating with a suction and discharge mechanism for sucking and discharging gas, the dispensing tip is supported by being detachably attached to the nozzle through an attachment opening at an upper end thereof, the plug is disposed at a tip end of the nozzle such that the electrode array element is located inside the dispensing tip, and the container group further includes a tip storing section for storing the dispensing tip such that the dispensing tip is attachable to the nozzle.

6. The device for electrical measurement of a target chemical substance according to claim 3, wherein the dispensing element includes: a thin tube having the mouth portion; a thick tube communicating with the thin tube; and a storage tube communicating with the thick tube, the electrode array section is disposed in the thick tube, and the storage tube is disposed so as to have a capacity capable of storing all the liquid introduced into the thick tube from the mouth portion.

7. The device for electrical measurement of a target chemical substance according to claim 3, wherein the processing head includes a magnetic force mechanism capable of applying a magnetic force to an inside of the attached dispensing element and removing the magnetic force therefrom and/or a temperature raising and lowering body for raising and lowering a temperature according to a signal from an outside, disposed so as to be close to or be able to approach an outside of the dispensing element or the electrode array element attached to the processing head, and the magnetic force mechanism and/or the temperature raising and lowering body are controlled by a control section.

8. The device for electrical measurement of a target chemical substance according to claim 1, wherein two non-wetted electrodes for application of an external electric field are disposed in the measurement container or the liquid storing section so as to sandwich an inside of the measurement container or an inside of the liquid storing section such that an electric field can be externally applied to the inside of the measurement container or the liquid storing section while the non-wetted electrodes are not in contact with liquid stored in the measurement container or the liquid storing section.

9. The device for electrical measurement of a target chemical substance according to claim 8, wherein the processing head includes a magnetic force mechanism capable of applying a magnetic force to an inside of the attached dispensing element and removing the magnetic force therefrom and/or a temperature raising and lowering body for raising and lowering a temperature according to a signal from an outside, disposed so as to be close to or be able to approach an outside of the dispensing element or the electrode array element attached to the processing head, and the magnetic force mechanism and/or the temperature raising and lowering body are controlled by a control section.

Description

BRIEF DESCRIPTION OF DRAWINGS

(1) FIG. 1 is a block diagram illustrating a device for electrical measurement of a target chemical substance according to an embodiment of the present invention.

(2) FIG. 2 is a perspective view illustrating an external appearance of a device illustrating First Example obtained by further embodying the device for electrical measurement of a target chemical substance according to the embodiment of the present invention illustrated in FIG. 1.

(3) FIG. 3 is an enlarged plan view illustrating an electrode array element illustrated in the device for electrical measurement of a target chemical substance according First Example illustrated in FIG. 2.

(4) FIG. 4 is a perspective view illustrating a main part of the device for electrical measurement of a target chemical substance according First Example illustrated in FIG. 2.

(5) FIG. 5 is a perspective view illustrating another example of the device for electrical measurement of a target chemical substance according to First Example illustrated in FIG. 2.

(6) FIG. 6 is an operation explanatory diagram of the device for electrical measurement of a target chemical substance according First Example illustrated in FIG. 3.

(7) FIG. 7 is a schematic view illustrating another example of a liquid storing section or a measurement container of the device for electrical measurement of a target chemical substance according First Example illustrated in FIG. 2.

(8) FIG. 8 is a perspective view illustrating an external appearance of a device illustrating Second Example obtained by further embodying the device for electrical measurement of a target chemical substance according to the embodiment of the present invention illustrated in FIG. 1.

(9) FIG. 9 is a perspective view illustrating a main part of the device for electrical measurement of a target chemical substance according Second Example illustrated in FIG. 8.

(10) FIG. 10 is an enlarged plan view illustrating an electrode array element illustrated in the device for electrical measurement of a target chemical substance according Second Example illustrated in FIG. 9.

(11) FIG. 11 is an operation explanatory diagram of the device for electrical measurement of a target chemical substance according Second Example illustrated in FIG. 9.

(12) FIG. 12 is an operation explanatory diagram of the device for electrical measurement of a target chemical substance according Second Example illustrated in FIG. 9.

(13) FIG. 13 is a perspective view illustrating a device for electrical measurement of a target chemical substance according to Third Example of the present invention.

(14) FIG. 14 is an enlarged plan view illustrating an electrode array element illustrated in the device for electrical measurement of a target chemical substance according Third Example illustrated in FIG. 13.

DESCRIPTION OF EMBODIMENTS

(15) Subsequently, a device for electrical measurement of a target chemical substance 10 according to an embodiment of the present invention will be described with reference to FIGS. 1 to 6.

(16) FIG. 1 is a block diagram illustrating the device for electrical measurement 10.

(17) The device for electrical measurement 10 roughly includes: for example, a container group 3 including storing section groups 3.sub.1 to 3.sub.n (n is equal to the number of the electrode array elements) in which a plurality of storing sections storing various solutions, an electrode array element described below, and various dispensing tips, arrayed in a row in a Y-axis direction is arrayed inn rows in an X-axis direction on a stage; a processing head 7 disposed so as to be movable in a relatively horizontal direction with respect to the container group 3, for example, in the Y-axis direction, and including electrode array element support sections 4.sub.1 to 4.sub.n which can sequentially selectively support either one or more (n in this example) electrode array elements 2.sub.1 to 2.sub.n or dispensing elements 8.sub.1 to 8.sub.n a tip end portion of which can be inserted into each of the storing sections, or can support both thereof overlappingly; a support section moving mechanism 5 for making the electrode array element support sections 4.sub.1 to 4.sub.n disposed in the processing head 7 relatively movable with respect to the container group 3; a CPU+program+memory 9 for performing information processing for various kinds of control; and an operation panel 14 for performing operation such as an instruction of a user to the CPU+program+memory 9. Examples of the various solutions include a sample solution which can contain a target chemical substance, a solution of an intercalating agent or an electrically active substance, a detergent, a buffer solution, a dissociated solution, a solution for extraction, a solution for PCR, and other various reagents.

(18) The processing head 7 further includes an array element support 4 in which one or more electrode array element support sections 4.sub.1 to 4.sub.n which sequentially selectively support either the electrode array elements 2.sub.1 to 2.sub.n or the dispensing elements 8.sub.1 to 8.sub.n or support both thereof overlappingly are arrayed at a predetermined pitch; a suction and discharge mechanism 83 for sucking and discharging gas with respect to the dispensing elements 8.sub.1 to 8.sub.n; applying units 62.sub.1 to 62.sub.n for applying a predetermined voltage to a test substance fixed to an electrode disposed in each of the electrode array elements 2.sub.1 to 2.sub.n; and measurement sections 64.sub.1 to 64.sub.n capable of measuring an electric signal from the electrode generated by applying the predetermined voltage to the test substance.

(19) Each of the storing section groups 3.sub.1 to 3.sub.n of the container group 3 at least includes: a tip and the like storing section group 3a storing the dispensing elements 8.sub.1 to 8.sub.n and the electrode array elements 2.sub.1 to 2.sub.n such that the dispensing elements 8.sub.1 to 8.sub.n and the electrode array elements 2.sub.1 to 2.sub.n can be supported by the electrode array element support sections 4.sub.1 to 4.sub.n; a liquid storing section group 3b storing various liquids; and a measurement container 3c for measuring the electrode array elements, including antipoles 63.sub.1 to 63.sub.n at an inner bottom thereof and disposed so as to be able to come into contact with liquid stored.

(20) The support section moving mechanism 5 includes: a processing head moving mechanism 52 for making it possible to relatively move the processing head 7 with respect to the container group 3 in the Y-axis direction; and a Z-axis moving mechanism 53 as a part of the support section moving mechanism 5 for making it possible to move the electrode array element support sections 4.sub.1 to 4.sub.n which sequentially selectively can support either the electrode array elements 2.sub.1 to 2.sub.n or the dispensing elements 8.sub.1 to 8.sub.n or can support both thereof overlappingly with respect to the processing head 7 in the Z-axis direction.

(21) The CPU+program+memory 9 includes: a reaction control section 91 for instructing the suction and discharge mechanism 83, the processing head moving mechanism 52, the Z-axis moving mechanism 53, a temperature control section, and the like to perform suction and discharge of the liquid stored in a predetermined liquid storing section group 3b by the dispensing elements 8.sub.1 to 8.sub.n, attachment of the dispensing elements 8.sub.1 to 8.sub.n to the electrode array element support sections 4.sub.1 to 4.sub.n, support of the dispensing elements 8.sub.1 to 8.sub.n by the electrode array element support sections 4.sub.1 to 4.sub.n, temperature control, and the like; a measurement control section 92 for instructing the applying units 62.sub.1 to 62.sub.4, the measurement section 64, the Z-axis moving mechanism 53, and the processing head moving mechanism 52 to perform measurement; and an analysis unit 93 for converting analog data measured by the measurement section into digital data and analyzing the data.

(22) The electrode array element support sections 4.sub.1 to 4.sub.n can support the electrode array elements 2.sub.1 to 2.sub.n and the dispensing elements 8.sub.1 to 8.sub.n, respectively, and are electrically connected to the electrodes disposed in the electrode array elements 2.sub.1 to 2.sub.n, respectively. As described below, in a case where the dispensing elements 8.sub.1 to 8.sub.n are dispensing tips, each of the electrode array element support sections 4.sub.1 to 4.sub.n includes at least a nozzle and a plug as the suction and discharge mechanism 83. In a case where the dispensing elements 8.sub.1 to 8.sub.n are deformable dispensing tips, each of the electrode array element support sections 4.sub.1 to 4.sub.n includes a support for supporting the deformable dispensing tip in place of the nozzle. In this case, the suction and discharge mechanism 83 includes a movable member for deforming the deformable dispensing tip.

(23) Subsequently, a device for electrical measurement of a target chemical substance 100 according to First Example of the embodiment of the present invention will be described with reference to FIGS. 2 to 7.

(24) The device for electrical measurement of a target chemical substance 100 is incorporated in a housing 11. As illustrated in the figures, the housing 11 includes a main body 12 in which a processing head 71 and a container group 31 as main parts of the device 100 are incorporated, and a lid 13 disposed so as to be able to open and close on an upper side. A reference numeral 14 denotes a touch panel type tablet connected to a main body as an operation panel wirelessly. Reference numerals 21.sub.3 and 21.sub.4 denote electrode array elements attached to the processing head 71.

(25) FIG. 3 is an enlarged view of the electrode array element 21.sub.4 of the device for electrical measurement of a target chemical substance 100 illustrated in FIG. 2.

(26) The electrode array element 21.sub.4 includes: a thin plate-shaped base 21d formed of an insulator such as glass, ceramic, or resin; one or more (12 in this example) electrodes 21a which are disposed on the base 21d and to each of which a test substance having a bonding property to a target chemical substance in a solution stored in the liquid storing section group 31b or a measurement container 31c is fixed; an electrode array section 21e in which the electrodes 21a are arrayed and which is disposed on a lower side of the base 21d; one or more (12 in this example) terminals 21b disposed on the base 21d so as to correspond to the electrodes 21a and electrically connected to the electrodes 21a by a conductive wire 21c; a terminal array section 21f in which the terminals 21b are arrayed and which is disposed on an upper side of the base 21d; and two protruding portions 21g horizontally protruding from both sides of the base 21d on an upper side of the base 21d, lower than the terminal array section 21f. The conductive wire 21c is embedded in a base or a layered portion covered with or formed of an insulator.

(27) The base 21d is formed into a thin rectangular parallelepiped shape having a predetermined height, for example, 1 cm to 10 cm, a predetermined width, for example, 1 cm to 10 cm, and a predetermined thickness shorter than the predetermined height and the predetermined width, for example, 5 mm or less. That is, the predetermined thickness is shorter than the predetermined height and the predetermined width.

(28) FIG. 4 more specifically illustrates the main part of the device for electrical measurement of a target chemical substance 100 illustrated in FIG. 2, extracted from the housing 11.

(29) The device for electrical measurement of a target chemical substance 100 roughly includes: a container group 31 including cartridge containers 31.sub.1 to 31.sub.4 in which a plurality of storing sections arrayed in a row so as to extend in the Y-axis direction is arrayed in a plurality of rows (four rows in this example) in the X-axis direction; a processing head 71 disposed so as to be relatively movable in a horizontal direction with respect to the container group 31, for example, in the Y-axis direction, and including electrode array element support sections 41.sub.1 to 41.sub.4 which can sequentially selectively support a plurality of (four in this example) electrode array elements 21.sub.1 to 21.sub.4 and dispensing tips 81.sub.1 to 81.sub.4; and the processing head moving mechanism 52 and a Z-axis moving mechanism 531 capable of moving the electrode array element support sections 4.sub.1 to 4.sub.n in the Z-axis direction as the support section moving mechanism 5 for relatively moving the electrode array element support sections 41.sub.1 to 41.sub.4 which can selectively support the electrode array elements 21.sub.1 to 21.sub.4 or the dispensing tips 81.sub.1 to 81.sub.4 disposed in the processing head 71 with respect to the container group 31 in the Y-axis direction.

(30) As illustrated in conjunction with FIG. 5, the cartridge containers 31.sub.1 to 31.sub.4 of the container group 31 (31) are formed in a plurality of rows (four rows in this example), and each of the cartridge containers 31.sub.1 to 31.sub.4 includes: a tip and the like storing section group 31a; a liquid storing section group 31b; a sealing lid storing section 31k; a measurement container 31c; a substrate 31e having a rectangular flat surface from which these storing section groups protrude downward; and a partition wall 31d disposed so as to extend upward along one edge extending in a longitudinal direction of the substrate 31e for preventing entry of splashes from an adjacent cartridge container. The liquid storing section group 31b includes a sample storing section 31g and a reaction container 31f (a difference from FIG. 4). A reference numeral 31h denotes a two-dimensional barcode for displaying specimen information and test information on the cartridge containers 31.sub.1 to 31.sub.4. Note that, in this example, the tip and the like storing section group 31a and the liquid storing section group 31b are formed separately.

(31) In the processing head 71, the electrode array element support sections 41.sub.1 to 41.sub.4 capable of sequentially selectively supporting the electrode array elements 21.sub.1 to 21.sub.4 and the dispensing tips 81.sub.1 to 81.sub.4 are arrayed in the X-axis direction at the same pitch as an array pitch of the cartridge containers 31.sub.1 to 31.sub.4 in the X-axis direction. The electrode array elements 21.sub.1 to 21.sub.4 are supported such that a normal direction of an array surface of the base 21d on which the electrode array section 21e and the terminal array section 21f of each of the electrode array elements 21.sub.1 to 21.sub.4 are formed faces the X-axis direction.

(32) Array element support 41 includes: a plurality of (four in this example) nozzles 83a attachable to the dispensing tips 811 to 814 and communicating with a suction and discharge mechanism; a plug 41a fitted with the terminal array section 21f of each of the electrode array elements 211 to 214 so as to be electrically connectable to each terminal 21b and having an elongated hole-shaped opening in the Y-axis direction; two gripping sections 41b capable of holding the two protruding portions 21g protruding in the Y-axis direction from each of the electrode array elements 211 to 214 fitted with the plug 41a and disposed with a gap in the Y-axis direction; an arm member 41c for supporting the two gripping sections 41b; and an X-axis movable support section 41d for supporting the arm member 41c such that the arm member 41c can slide by a predetermined distance in the X-axis direction based on an instruction from an outside.

(33) The processing head 71 further includes a suction and discharge mechanism 831 communicating with the nozzle 83a. The suction and discharge mechanism 831 includes: a cylinder 83b having a plunger slidably disposed therein; a detachment mechanism 85 including a cutout portion 85a having a larger inner diameter than the nozzle 83a and having a smaller inner diameter than an outer diameter of a storage tube above a thick tube of each of the dispensing tips 811 to 814, and a detachment member 85b including the cutout portion 85a; the array element support 41 for supporting a cylinder drive mechanism for reciprocating the cylinder 83b and the plunger sliding in the cylinder 83b; and the Z-axis moving mechanism 531 for making the array element support 41 movable in the Z-axis direction.

(34) When the cylinder drive mechanism pushes down the plunger so as to be in a range beyond a suction and discharge range of the plunger, the cylinder drive mechanism pushes down also an upper end of the detachment member 85b, and the detachment member 85b thereby detaches the dispensing tips 81.sub.1 to 81.sub.4 from the nozzles 83a. As the detachment member for the electrode array elements 21.sub.1 to 21.sub.4, the gripping section 41b is used. Also for the arm member 41c supporting the gripping section 41b, when the cylinder drive mechanism pushes down the plunger so as to be in a range beyond a suction and discharge range of the plunger, the cylinder drive mechanism pushes down also an upper end of the arm member 41c, and the electrode array elements 21.sub.1 to 21.sub.4 are thereby detached from the plugs 41a.

(35) The processing head 71 further includes a magnetic force mechanism 87 for applying a magnetic force to an inside of each of the dispensing tips 81.sub.1 to 81.sub.4 attached to the nozzle. For example, the magnetic force mechanism 87 includes at least: n (four in this example) permanent magnets 871 arrayed at intervals corresponding to the array of the dispensing tips 81.sub.1 to 81.sub.4; a magnet array member 872 for supporting the n permanent magnets 871; and an actuator 873 disposed in the Y-axis direction so as to move the magnet array member 872 forward and backward with respect to the dispensing tips 81.sub.1 to 81.sub.4 in the Y-axis direction, having a ball screw having one end pivotally supported by the magnet array member and the other end pivotally supported by a ball screw shaft support plate, and a motor for rotationally driving a nut portion screwed with the ball screw built-in, and supported by the processing head 71 (refer to FIG. 12). The processing head 71 further includes: a temperature raising and lowering body 88a for controlling the temperature of a liquid sucked into the dispensing tips 81.sub.1 to 81.sub.n; a raising and lowering body advancing and retracting drive mechanism 88b for advancing or retracting the temperature raising and lowering body 88a so as to make the temperature raising and lowering body 88a approach the dispensing tips 81.sub.1 to 81.sub.n and the like; and a temperature controller 88c for controlling the temperature rising and falling of the temperature raising and lowering body 88a.

(36) As illustrated in FIG. 5, the tip and the like storing section group 31a is stored in a first storing section such that each of the electrode array elements 21.sub.1 to 21.sub.4 can be fitted with the plug 41a of the array element support 41, the protruding portion 21g can be gripped by the gripping section 41b, and the terminal array section 21f of each of the electrode array elements 21.sub.1 to 21.sub.4 is exposed to an upper side of the storing section with an elongated hole-shaped opening in the Y-axis direction. The dispensing tips 81.sub.1 to 81.sub.4 are stored in a second storing section with an attachment opening 81c facing up such that the dispensing tips 81.sub.1 to 81.sub.4 are attachable to the nozzles 83a of the electrode array element support sections 41.sub.1 to 41.sub.4, respectively. A piercing tip 86 is stored in a third storing section such that the piercing tip 86 is attachable to the nozzle 83a.

(37) As illustrated in FIG. 5, the dispensing tip 81 includes: a tapered cylindrical thin tube 81a; a thick tube 81b communicating with the thin tube 81a through a transition portion 81e; a mouth portion 81f which is disposed at a tip end of the thin tube 81a, can be inserted into a container, and sucks and discharges liquid; an attachment opening 81c disposed above the thick tube 81b and attachable to the nozzle 83a (refer to FIG. 6) communicating with a suction and discharge mechanism for sucking and discharging gas; a plurality of vertically extending ridges 81d disposed along an outer periphery of an outer surface of a storage tube on an upper side of the thick tube 81b having the attachment opening 81c; and the transition portion 81e disposed between the thin tube 81a and the thick tube 81b.

(38) The liquid storing section group 31b includes a plurality of (nine in this example) liquid storing sections capable of storing liquid. Specifically, the liquid storing section group 31b includes a sample storing section 31g (first liquid storing section) storing, for example, a food extract solution extracted from food as an extracted sample for a test, and a liquid storing section group storing a plurality of liquid storing sections storing various cleaning buffer solutions.

(39) The measurement container 31c has an elongated hole-shaped opening with a longitudinal direction in the Y-axis direction, and includes antipoles 63.sub.1 to 63.sub.4 at an inner bottom portion thereof so as to be able to come into contact with liquid stored in the container 31c. The measurement container 31c is formed so as to have an inner width longer than the predetermined width of the base 21d, for example, 1 cm to 10 cm, for example, an inner width of 1.1 cm to 11 cm, and an inner depth longer than the predetermined thickness but shorter than the predetermined width, for example, an inner depth of 1 mm to 10 mm. As a result, even a liquid having a small volume can come into contact with the electrode.

(40) As illustrated in FIG. 6, on an outer wall of a side surface having the largest area among side surfaces of the measurement container 31c or in a wall thickness of the outer wall, two non-wetted electrodes 15 and 16 for application of an external electric field for applying Brownian motion to liquid or a solution stored in the measurement container 31c are disposed so as to sandwich the measurement container 31c and not to be in contact with the liquid stored therein. By applying an alternating high voltage between the electrodes, Brownian motion of the solution stored in the measurement container 31c can be activated, encounterability between the electrodes and a target substance in the solution can be enhanced, and a reaction can be accelerated.

(41) Subsequently, an operation in a case where the device for electrical measurement of a target chemical substance 100 and a method therefor according to First Example of the present embodiment are applied to detection of a specific food allergen on the display obligation 7 items (egg, milk, wheat, buckwheat, peanut, shrimp, and a crab) for four kinds of foods will be described with reference to FIG. 5.

(42) Two of the twelve electrodes 21a of the electrode array elements 211 to 214 are electrodes 21a for negative control and positive control. The electrode 21a for negative control is blocked such that an antigen or an antibody is not bonded to the electrode 21a. The electrode 21a for positive control necessarily generates a predetermined electric signal. An antibody (for example, an anti-wheat antibody or an anti-egg antibody) capable of capturing an allergen is fixed to each electrode 21a as a test substance. Furthermore, each liquid storing section of the liquid storing section group 31b includes, in advance, in order, two sets of a liquid storing section storing 100 L of a food extract solution extracted from food and three liquid storing sections each storing 200 L of cleaning buffer solution (1PBS 0.05% Tween). Note that an allergen in each food extract solution is labeled in advance by bonding the allergen to an electrochemically active substance such as a metal complex via an amino group, a carboxyl group, or the like included in the antigen.

(43) In step S1, the processing head moving mechanism 53 moves the processing head 7 in the Y-axis direction so as to located above the first storing section of the tip and the like storing section group 31a storing the electrode array elements 21.sub.1 to 21.sub.4. By lowering the plug 41a of each of the electrode array element support sections 41.sub.1 to 41.sub.4 disposed on the array element support 41 of the processing head 71 by the Z-axis moving mechanism 531, the terminal array section 21f of each of the electrode array elements 21.sub.1 to 21.sub.4 is fitted with and attached to the plug 41a. At this time, the gripping section 41b moves to a position retracted with respect to the plug 41a by the X-axis movable support member 41d. After each of the electrode array elements 21.sub.1 to 21.sub.4 is attached to the plug 41a, the X-axis movable support member 41d moves the electrode array elements 21.sub.1 to 21.sub.4 in the X-axis direction so as to pass over the plug 41a, and the protruding portions 21g of the electrode array elements 21.sub.1 to 21.sub.4 are gripped.

(44) In step S2, the Z-axis moving mechanism 531 raises the electrode array elements 21.sub.1 to 21.sub.4 attached to the electrode array element support sections 41.sub.1 to 41.sub.4 in the Z-axis direction. Thereafter, the processing head moving mechanism 52 moves the electrode array elements 21.sub.1 to 21.sub.4 in the Y-axis direction to locate the electrode array elements 21.sub.1 to 21.sub.4 above the sample storing section 31g storing the food extract solution. The Z-axis moving mechanism 531 lowers the electrode array elements 21.sub.1 to 21.sub.4, and suction is performed until reaching such a capacity that the electrode array section 21e of each of the electrode array elements 21.sub.1 to 21.sub.4 is immersed in the food extract solution to bring the electrode array elements 21.sub.1 to 21.sub.4 into contact with the food extract solution.

(45) In step S3, the Z-axis moving mechanism 531 raises the electrode array elements 21.sub.1 to 21.sub.4 in the Z-axis direction. Thereafter, the processing head moving mechanism 52 moves the electrode array elements 21.sub.1 to 21.sub.4 in the Y-axis direction above the liquid storing section 31b storing a cleaning liquid. The Z-axis moving mechanism 531 lowers the electrode array elements 21.sub.1 to 21.sub.4 to bring the electrode array section of each of the electrode array elements 21.sub.1 to 21.sub.4 into contact with the cleaning liquid for cleaning. Similarly, cleaning is repeated with respect to other cleaning liquids.

(46) In step S4, the processing head moving mechanism 52 moves the electrode array elements 21.sub.1 to 21.sub.4 in the Y-axis direction above the measurement container 31c. The Z-axis moving mechanism 531 lowers the electrode array elements 21.sub.1 to 21.sub.4 to bring the electrode array elements 21.sub.1 to 21.sub.4 into contact with the solution in the measurement container 31c. While the electrode array section of each of the electrode array elements 21.sub.1 to 21.sub.4 is immersed in the solution, the applying units 62.sub.1 to 62.sub.4 apply a voltage to an antibody bonding to the electrodes 21a via the counter electrode 63, and the measurement sections 64.sub.1 to 64.sub.4 measure the amount of the voltage. The measurement result is digitally converted, is then stored in a storage unit in the CPU+program+memory 9, and is analyzed by the analysis unit 93.

(47) As illustrated in FIG. 6, at least in the measurement step of step S4, by applying the high voltage (1 kV) with an alternating voltage 17 having a predetermined frequency (for example, 20 Hz) through the non-wetted electrodes 15 and 16 for application of an external electric field disposed so as to sandwich the measurement container 31c on an outer surface having the largest area in the measurement container 31c, Brownian motion is applied to an inside of the measurement container 31c to stir the electrolytic solution. A target chemical substance is thereby stirred in a solution to enhance encounterability with a test substance fixed to each of the electrodes of the electrode array elements 21.sub.1 to 21.sub.4 and to accelerate a reaction.

(48) FIG. 7 illustrates that each liquid storing section belonging to the liquid storing section group 31b is formed into a rectangular tube shape, and non-wetted electrodes 151 to 156 for application of an external electric field for applying an electric field for stirring to an inside of each liquid storing section are disposed on an outer surface of each rectangular tubular liquid storing section so as to stir the liquid.

(49) Next, a device for electrical measurement of a target chemical substance 101 according to Second Example of the embodiment of the present invention will be described with reference to FIGS. 8 to 11. The basic structure of the device for electrical measurement of a target chemical substance 101 is as illustrated in the block diagram of FIG. 1, but details thereof are as follows.

(50) FIG. 8 illustrates an external appearance of the device for electrical measurement of a target chemical substance 101.

(51) The device for electrical measurement of a target chemical substance 101 is incorporated in a housing 11 in a similar manner to the above device for electrical measurement of a target chemical substance 100 according to First Example. The housing 11 includes a main body 12 in which a processing head 72 and a container group 32 as main parts of the device 101 are incorporated, and a lid 13 disposed so as to be able to open and close on an upper side. A reference numeral 14 denotes a touch panel type tablet connected to a main body as an operation panel wirelessly. A reference numeral 81.sub.4 denotes a dispensing tip including an electrode array element 21.sub.4.

(52) FIG. 9 more specifically illustrates the main part of the device for electrical measurement of a target chemical substance 101 illustrated in FIG. 8, extracted from the housing 11.

(53) The device for electrical measurement of a target chemical substance 101 roughly includes: a container group 32 including cartridge containers 32.sub.1 to 32.sub.4 in which a plurality of storing sections arrayed in a row so as to extend in the Y-axis direction is arrayed in a plurality of rows (four rows in this example) in the X-axis direction; a processing head 72 disposed so as to be relatively movable in a horizontal direction with respect to the container group 32, for example, in the Y-axis direction, and including electrode array element support sections 42.sub.1 to 42.sub.4 which can support a plurality of (four in this example) electrode array elements 23.sub.1 and 23.sub.4 and dispensing tips 81.sub.1 to 81.sub.4 in this order; and a processing head moving mechanism 52 and a Z-axis moving mechanism 531 capable of moving the electrode array element support sections 42.sub.1 to 42.sub.4 in the Z-axis direction as a support section moving mechanism 5 capable of relatively moving the electrode array element support sections 42.sub.1 to 42.sub.4 which can overlappingly support the electrode array elements 23.sub.1 to 23.sub.4 and the dispensing tips 81.sub.1 to 81.sub.4 disposed in the processing head 72 with respect to the container group 32 in the Y-axis direction.

(54) As illustrated in conjunction with FIG. 11, the cartridge containers 32.sub.1 to 32.sub.4 of the container group 32 are formed in a plurality of rows (four rows in this example), and each of the cartridge containers 32.sub.1 to 32.sub.4 includes: a tip and the like storing section group 32a; a liquid storing section group 32b; a lid storing section 32k for storing a sealing lid used for sealing an opening of a measurement container 32c; the measurement container 32c; a substrate 32e having a rectangular flat surface from which these storing section groups protrude downward; and a partition wall 32d disposed so as to extend upward along one edge extending in a longitudinal direction of the substrate 32e for preventing entry of splashes from an adjacent cartridge container. A reference numeral 32h denotes a two-dimensional barcode for displaying specimen information and test information on the cartridge containers 32.sub.1 to 32.sub.4. Note that, in this example, the tip and the like storing section group 32a and the liquid storing section group 32b are formed separately.

(55) In the processing head 72, nozzles 83a as the electrode array element support sections 42.sub.1 to 42.sub.4 capable of supporting the dispensing tips 81.sub.1 to 81.sub.4 alone or the electrode array elements 23.sub.1 to 23.sub.4 and the dispensing tips 81.sub.1 to 81.sub.4 while the electrode array elements 23.sub.1 to 23.sub.4 and the dispensing tips 81.sub.1 to 81.sub.4 are attached in this order and the electrode array elements 23.sub.1 to 23.sub.4 are enclosed in thick tubes of the dispensing tips 81.sub.1 to 81.sub.4 are arrayed in the X-axis direction at the same pitch as the array pitch of the cartridge containers 32.sub.1 to 32.sub.4 in the X-axis direction. Note that the electrode array elements 23.sub.1 to 23.sub.4 are disposed such that a normal direction of a plane having the largest area of the base 23d faces the X-axis direction.

(56) FIG. 10 is an enlarged view of the electrode array element 23 of the device for electrical measurement of a target chemical substance 101 illustrated in FIG. 9.

(57) The electrode array element 23 includes: a thin plate-shaped base 23d formed of an insulator such as glass, ceramic, or resin; one or more (12 in this example) electrodes 23a which are disposed on the base 23d and to each of which a test substance having a bonding property to a target chemical substance in a solution stored in the liquid storing section group 32b or the measurement container 32c is fixed; an electrode array section 23e in which the electrodes 23a are arrayed and which is disposed on a lower side of the base 23d; one or more (12 in this example) terminals 23b disposed on the base 23d so as to correspond to the electrodes 23a and electrically connected to the electrodes 23a by a conductive wire 23c; and a terminal array section 23f in which the terminals 23b are arrayed and which is disposed on an upper side of the base 23d. The conductive wire 23c is embedded in a base or a layered portion covered with or formed of an insulator.

(58) Returning to FIG. 9, the electrode array element support sections 42.sub.1 to 42.sub.4 include a plurality of (four in this example) nozzles 84a attachable to the dispensing tips 81.sub.1 to 81.sub.4 and the electrode array elements 23.sub.1 to 23.sub.4 and communicating with a suction and discharge mechanism. Each of the nozzles 84a includes a plug fitted with the terminal array section 23f of each of the electrode array elements 23.sub.1 to 23.sub.4 so as to be electrically connectable to each terminal 23b and having an elongated hole-shaped opening in the Y-axis direction.

(59) The processing head 72 further includes a suction and discharge mechanism 832 communicating with the nozzle 84a. The suction and discharge mechanism 832 includes: a cylinder 84b having a plunger slidably disposed therein; a detachment mechanism 85 including a cutout portion 85a having a larger inner diameter than the nozzle 84a and having a smaller inner diameter than an outer diameter of a storage tube above a thick tube of each of the dispensing tips 81.sub.1 to 81.sub.4, and a detachment member 85b including the cutout portion 85a; an array element support 42 for supporting a cylinder drive mechanism for reciprocating the cylinder 84b and the plunger sliding in the cylinder 84b; and the Z-axis moving mechanism 531 for making the array element support 42 movable in the Z-axis direction.

(60) Note that the detachment member 85b, the magnetic force mechanism 44, the temperature raising and lowering body 481, the dispensing tip 81, and the like have already been described above, and therefore explanation thereof is omitted.

(61) As illustrated in FIG. 9, the tip and the like storing section group 32a is stored in a first storing section such that each of the electrode array elements 23.sub.1 to 23.sub.4 can be fitted with a plug disposed at a lower end of the nozzle 84a as the electrode array element support section 43, and the terminal array section 23f of each of the electrode array elements 23.sub.1 to 23.sub.4 is exposed to an upper side of the storing section with an elongated hole-shaped opening in the Y-axis direction. The dispensing tips 81.sub.1 to 81.sub.4 are stored in a second storing section with an attachment opening 81c facing up such that the dispensing tips 81.sub.1 to 81.sub.4 are attachable to the nozzles 84a of the electrode array element support section 43. A piercing tip 86 is stored in a third storing section such that the piercing tip 86 is attachable to the nozzle 84a. Note that the dispensing tip 81 has been described above, and therefore description thereof is omitted here.

(62) The liquid storing section group 32b includes a plurality of (nine in this example) liquid storing sections capable of storing liquid. More specifically, as illustrated in conjunction with FIG. 11, for example, the liquid storing section group 32b includes: a sample storing section 32g (first liquid storing section, counting method is the same as in the liquid storing section group 32b) for storing a sample collected from a subject, such as serum, plasma, whole blood, or body fluid in order to specify bacteria or viruses of infectious diseases which the subject has developed; a magnetic particle suspension storing section used for collecting bacteria and the like (second liquid storing section); a storing section for storing 40 L of a protein denaturating enzyme solution (Lysis 1) for crushing bacteria and cells (third liquid storing section); a storing section for storing 200 L of a proteolytic enzyme solution (Lysis 2) (fourth liquid storing section); a storing section for storing 500 L of a separated extraction solution (bonding buffer solution, NaCl, SDS, and isopropanol) (fifth liquid storing section and sixth liquid storing section); a storing section for storing 700 L of cleaning liquid (water and isopropanol) (seventh liquid storing section); a dissociated solution (eighth liquid storing section); and a temperature-controllable reaction container 32f (ninth liquid storing section).

(63) Furthermore, the measurement container 32c also serves as a PCR container capable of performing temperature control so as to make amplification of an extracted nucleic acid possible. The liquid storing section group 32b further includes a lid storing section 32k for storing a sealing lid which is used for sealing the measurement container 32c and which can be pierced.

(64) Subsequently, the device for electrical measurement of a target chemical substance 101 according to Second Example of the present embodiment and an operation of a method therefor will be described with reference to FIGS. 11 and 12. Here, whether there is a bacterium to cause a predetermined infectious disease in a specimen of a subject is tested. Steps S11 to S19 correspond to a nucleic acid extraction step.

(65) In step S11, by operating a touch panel or the like of the operation panel 14, an instruction to start test processing of an infectious disease is given.

(66) In step S12, an extraction/reaction control section 91 disposed in the CPU+program+memory 9 (information processing device) of the device for electrical measurement of a target chemical substance 101 instructs the processing head moving mechanism 52 to move the processing head 72 in the Y-axis direction above the piercing tip 86 stored in the tip and the like storing section group 32a of each of the cartridge containers 321 to 324. Thereafter, by driving the Z-axis moving mechanism 531, the nozzle 84a is lowered, is attached to the piercing tip 86, and is raised. The processing head moving mechanism 52 locates the piercing tip 86 above the second liquid storing section of the liquid storing section group 32b of the container group 32. The Z-axis moving mechanism 531 lowers the piercing tip 86, and thereby pierces a film covering an opening of the liquid storing section. Similarly, by moving the processing head 72 in the Y-axis direction, another liquid storing section and the reaction container 32f in the liquid storing section 32b are also pierced sequentially.

(67) In step S13, the piercing tip 86 is detached into the tip and the like storing section group 32a using the detachment mechanism 85. Thereafter, the processing head 72 is moved to the tip and the like storing section group 32a storing the dispensing tips 81.sub.1 to 81.sub.4 in the Y-axis direction. The Z-axis moving mechanism 531 lowers the nozzles 84a, and the dispensing tips 81.sub.1 to 81.sub.4 are thereby attached to the processing head 72 via the nozzles 84a. Subsequently, the Z-axis moving mechanism 531 raises the dispensing tips 81.sub.1 to 81.sub.4, and then the processing head moving mechanism 52 moves the dispensing tips 81.sub.1 to 81.sub.4 in the Y-axis to the sample storing section 32g (first liquid storing section) storing a parent specimen. Thereafter, the Z-axis moving mechanism 531 lowers mouth portions 81f of the dispensing tips 81.sub.1 to 81.sub.4 for insertion. The suction and discharge mechanism 832 raises and lowers a plunger. The sample liquid stored in the sample storing section 32g is thereby repeatedly sucked and discharged such that the sample is suspended in the liquid. Thereafter, the sample liquid is sucked into the dispensing tips 81.sub.1 to 81.sub.4. The processing head 72 moves the sample liquid in the Y-axis direction while the sample liquid is sucked into the dispensing tips 81.sub.1 to 81.sub.4, and moves the sample liquid to a third liquid storing section storing Lysis 1 (enzyme) as a separated extraction solution. The thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4 is inserted into the third liquid storing section through a hole of a pierced film. Suction and discharge for stirring the sample liquid and the Lysis 1 are repeated.

(68) In step S14, the whole amount of the stirred liquid is sucked by the dispensing tips 811 to 814, is stored in the reaction container 32f (ninth liquid storing section) formed of a reaction tube held in a storing hole set at 55 C. by constant temperature controller, and is incubated. A protein contained in the sample is thereby denatured. After a lapse of predetermined time, the processing head moving mechanism 52 moves the dispensing tips 811 to 814 which have sucked the reaction liquid to a fourth liquid storing section of each of the cartridge containers 321 to 324. The Z-axis moving mechanism 531 and the suction and discharge mechanism 832 mix the reaction liquid with the whole amount of Lysis 2 stored in the fourth liquid storing section to lower the molecular weight of the protein.

(69) In step S15, a bonding buffer solution as a separated extraction solution stored in the fifth liquid storing section and the reaction solution are stirred to further dehydrate a solubilized protein, and a nucleic acid or a fragment thereof is dispersed in the solution.

(70) In step S16, using the dispensing tips 81.sub.1 to 81.sub.4, the thin tube is inserted into the fourth liquid storing section through the hole of the film, and the whole amount is sucked. The Z-axis moving mechanism 531 raises the dispensing tips 81.sub.1 to 81.sub.4, and transfers the reaction solution to the second liquid storing section. By repeating suction and discharge of a magnetic particle suspension liquid and the reaction solution stored in the second liquid storing section using the suction and discharge mechanism 832, mixing and stirring are performed. A cation structure in which a Na+ ion is bonded to a hydroxy group formed on a surface of silica or the like covering each magnetic particle contained in the magnetic particle suspension is formed. Therefore, negatively charged DNA is captured by magnetic particles.

(71) In step S17, as illustrated in FIG. 12, by making the magnet 871 of the magnetic force mechanism 87 approach the thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4, the magnetic particles are adsorbed by an inner wall of the thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4. The Z-axis moving mechanism 531 raises the magnetic particles while the magnetic particles are adsorbed by the thin tubes 81a of each of the dispensing tips 81.sub.1 to 81.sub.4. The processing head moving mechanism 52 moves the dispensing tips 81.sub.1 to 81.sub.4 from the second liquid storing section to the sixth liquid storing section, and the thin tube is inserted into the sixth liquid storing section through the hole of the pieced film.

(72) While a magnetic force to an inside of the thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4 is removed by separating the magnet 871 of the magnetic force mechanism 87 from the thin tube, a cleaning liquid 1 (NaCl, SDS, and isopropanol) stored in the sixth liquid storing section is repeatedly sucked and discharged. The magnetic particles are thereby detached from the inner wall, are stirred in the cleaning liquid 1, and cleaning is thereby performed. Thereafter, while the magnetic particles are adsorbed by the inner wall of the thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4 by making the magnet of the magnetic force mechanism 87 approach the thin tube again, the Z-axis moving mechanism 531 raises the dispensing tips 81.sub.1 to 81.sub.4, and then the processing head moving mechanism 52 moves the dispensing tips 81.sub.1 to 81.sub.4 from the sixth liquid storing section to the seventh liquid storing section.

(73) In step S18, the thin tube of each of the dispensing tips 81.sub.1 to 81.sub.4 is inserted through the hole of the film using the Z-axis moving mechanism 531. While a magnetic force to an inside of the thin tube of each of the dispensing tips 81.sub.1 to 81.sub.4 is removed by separating the magnet of the magnetic force mechanism 87 from the thin tube, a cleaning liquid 2 (isoproanol) stored in the seventh liquid storing section is repeatedly sucked and discharged. The magnetic particles are thereby stirred in the liquid, NaCl and SDS are removed, and cleaning is performed. Thereafter, while the magnetic particles are adsorbed by the inner wall of the thin tube of each of the dispensing tips 81.sub.1 to 81.sub.4 by making the magnet of the magnetic force mechanism 87 approach the thin tube again, the Z-axis moving mechanism 531 raises the dispensing tips 81.sub.1 to 81.sub.4, and then the processing head moving mechanism 52 moves the dispensing tips 81.sub.1 to 81.sub.4 from the seventh liquid storing section to the eighth liquid storing section storing distilled water.

(74) In step S19, the Z-axis moving mechanism 531 lowers the thin tube of each of the dispensing tips 81.sub.1 to 81.sub.4 through the hole. By repeating suction and discharge of the distilled water at a slow flow rate while the magnetic force is applied to an inside of the thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4, the cleaning liquid 2 (isopropanol) is replaced with the distilled water, and is removed. Thereafter, by sucking and discharging the magnetic particles repeatedly into distilled water as the dissociated solution stored in the eighth liquid storing section while the magnet of the magnetic force mechanism 87 is separated from the thin tube of each of the dispensing tips 81.sub.1 to 81.sub.4 and the magnetic force is removed, stirring is performed, and a nucleic acid retained by the magnetic particles or a fragment thereof is dissociated (eluted) from the magnetic particles into the liquid. Thereafter, by making the magnet 871 approach the thin tube 81a of each of the dispensing tips 81.sub.1 to 81.sub.4, a magnetic field is applied to an inside of the thin tube and the magnetic particles are adsorbed by the inner wall, and the liquid is left such that the extracted nucleic acid or the like is contained in the eighth liquid storing section. The processing head moving mechanism 52 moves the dispensing tips 81.sub.1 to 81.sub.4 to the eighth liquid storing section holding the cleaning liquid 3. By repeating suction and discharge with the magnetic field removed, the magnetic particles are discharged together with the cleaning liquid 3 while the magnetic particles are resuspended to clean the dispensing tips 81.sub.1 to 81.sub.4.

(75) Hereinafter, steps S20 to S25 correspond to a nucleic acid amplification and measurement step.

(76) In step S20, the processing head moving mechanism 52 moves the processing head 72. A solution containing a nucleic acid or the like stored in the eighth liquid storing section is sucked, is transferred to the PCR container 32c storing an amplification solution in advance, is discharged, and is introduced into the container. The PCR container 32c contains a primer for amplifying a characteristic base sequence portion of a target bacterium or a target virus of an infectious disease to be detected in advance. If the target bacterium or the like exists, the base sequence portion is amplified. The processing head moving mechanism 52 returns the dispensing tips 81.sub.1 to 81.sub.4 to tip and the like storing section 32a. The dispensing tips 81.sub.1 to 81.sub.4 are detached by the detachment member 85b. The nozzle 84a is moved again above the sealing lid storing section 32k. By lowering the nozzle 84a using the Z-axis moving mechanism 531, a recess on an upper side of the sealing lid is fitted with a lower end of the nozzle 84a for attachment. The Z-axis moving mechanism 531 lowers the sealing lid, and the sealing lid is fitted with and attached to an opening of the PCR container-measurement container 32c, and is sealed.

(77) In step S21, when amplification of a nucleic acid extracted by performing temperature control based on a PCR method is completed, the sealing lid is pierced by the piercing tip 86, and the measurement control section 92 instructs the processing head moving mechanism 52 to bring the processing head 72 above the storing section of the piercing tip 86 of the tip and the like storing section group 32a. The piercing tip 86 is detached by the detachment member. Furthermore, the processing head 72 is moved in the Y-axis direction above the storing sections of the electrode array elements 23.sub.1 to 23.sub.4. The Z-axis moving mechanism 531 lowers the nozzles 84a, and the electrode array elements 23.sub.1 to 23.sub.4 are attached to the plugs disposed at lower ends of the nozzles 84a. Subsequently, the Z-axis moving mechanism 531 raises the nozzles 84a. Thereafter, the processing head 72 is moved in the Y-axis direction above the storing sections of the dispensing tips 81.sub.1 to 81.sub.4, and is lowered such that the dispensing tips 81.sub.1 to 81.sub.4 are attached to the nozzles 84a while the electrode array elements 23.sub.1 to 23.sub.4 are attached to the nozzles 84a to form a combination state in which the electrode array elements 23.sub.1 to 23.sub.4 are supported inside the dispensing tips 81.sub.1 to 81.sub.4.

(78) In step S21, in that state, using the dispensing tips 81.sub.1 to 81.sub.4, a liquid storing section storing a solution of an electrochemically active substance such as an intercalating agent or a metal complex is prepared in the liquid storing section group or the like. The intercalating agent or the like is sucked from the liquid storing section. The processing head moving mechanism 52 is instructed to move the processing head 72 in the Y axis direction above the measurement container 32c. By lowering the dispensing tips 81.sub.1 to 81.sub.4 and the electrode array elements 23.sub.1 to 23.sub.4 by the Z-axis moving mechanism 531, the intercalating agent or the like is discharged, mixing with an amplified product is performed, and suction and discharge are repeated. A double stranded base sequence generated by hybridization between the test substance fixed to each electrode 23a of the electrode array elements 23.sub.1 to 23.sub.4 and a target chemical substance is labeled with the intercalating agent or the like. A change in an electric signal is measured by applying a predetermined voltage by the applying unit. Presence or absence and the amount of bonding between the amplified product and the test carrier fixed to each electrode are measured.

(79) Subsequently, a device for electrical measurement of a target chemical substance 102 according to Third Example of the embodiment of the present invention will be described with reference to FIG. 13. The basic structure of the device for electrical measurement of a target chemical substance 102 is as illustrated in the block diagram of FIG. 1, but details thereof are as follows.

(80) The device for electrical measurement of a target chemical substance 102 roughly includes: a container group 33 including cartridge containers 33.sub.1 to 33.sub.4 in which a plurality of storing sections arrayed in a row so as to extend in the Y-axis direction is arrayed in a plurality of rows (four rows in this example) in the X-axis direction; a processing head 73 (refer to FIG. 14) disposed so as to be relatively movable in a horizontal direction with respect to the container group 33, for example, in the Y-axis direction, and including electrode array element support sections 43.sub.1 to 43.sub.4 which can support a plurality of (four in this example) electrode array elements 22.sub.1 to 22.sub.4 described below; and a processing head moving mechanism 52 and a Z-axis moving mechanism 531 capable of moving the electrode array elements 22.sub.1 to 22.sub.4 or the dispensing tips 81.sub.1 to 81.sub.4 in the Z-axis direction as a support section moving mechanism 5 capable of relatively moving the electrode array element support sections 43.sub.1 to 43.sub.4 disposed in the processing head 73, that is, the electrode array elements 22.sub.1 to 22.sub.4 supported by the electrode array element support sections 43.sub.1 to 43.sub.4 with respect to the container group 33 in the Y-axis direction.

(81) As illustrated in FIG. 13, the cartridge containers 33.sub.1 to 33.sub.4 of the container group 33 are formed in a plurality of rows (four rows in this example), and each of the cartridge containers 33.sub.1 to 33.sub.4 includes: a tip and the like storing section group (not illustrated); a liquid storing section group 33b; a measurement container 33c; a substrate 33e having a rectangular flat surface from which these storing section groups protrude downward; and a partition wall 33d disposed so as to extend upward in a longitudinal direction of the substrate 33e for preventing entry of splashes from an adjacent cartridge container. Note that, in this example, the tip and the like storing section group 33a and the liquid storing section group 33b are formed separately. In FIG. 13, a reference numeral 33g denotes a sample storing section, and a reference numeral 33f denotes a reaction container.

(82) In the processing head 73, as the electrode array element support sections 43.sub.1 to 43.sub.4 capable of being attached to and supporting the electrode array elements 22.sub.1 to 22.sub.4, nozzles 83a (refer to FIG. 4) communicating with a suction and discharge mechanism 83 for sucking and discharging gas and plugs 43a are arrayed in the X-axis direction at the same pitch as an array pitch of the cartridge containers 33.sub.1 to 33.sub.4 in the X-axis direction.

(83) As illustrated in FIG. 13, each of the electrode array elements 22.sub.1 to 22.sub.4 includes, as a base, a dispensing tip 82 as a dispensing element and a plate 22d. The dispensing tip 82 includes: a tapered cylindrical thin tube 82a; a thick tube 82b communicating with the thin tube 82a through a transition portion 82e; a mouth portion 82f which is disposed at a tip end of the thin tube 82a, can be inserted into a container, and sucks and discharges liquid; an attachment opening 82c disposed in a storage tube above the thick tube 82b and attachable to the nozzle; a plurality of vertically extending ridges 82d disposed along an outer periphery of an outer surface on an upper side of the thick tube 82b having the attachment opening 82c; and a transition portion 82e disposed between the thin tube 82a and the thick tube 82b. An electrode array section 22e is disposed on the plate 22d inside the thick tube 82b of the dispensing tip 82, and the terminal array section 22f is disposed outside the dispensing tip 82 to form an electrode array plate 22 (refer to FIG. 14).

(84) FIG. 14 illustrates the electrode array plate 22 and the plug 43a as the electrode array element support sections 43.sub.1 to 43.sub.4.

(85) The electrode array plate 22 includes: the plate 22d formed of an insulator such as glass, ceramic, or resin as a part of a base; one or more (12 in this example) electrodes 22a which are disposed on the plate 22d and to each of which a test substance having a bonding property to a target chemical substance in a solution stored in the liquid storing section group 33b or the measurement container 33c is fixed; an electrode array section 22e in which the electrodes 22a are arrayed and which is disposed on one side of the thin plate-shaped plate 22d; one or more (12 in this example) terminals 22b disposed on the thin plate-shaped plate 22d so as to correspond to the electrodes 22a and electrically connected to the electrodes 22a by a conductive wire 22c; and a terminal array section 22f in which the terminals 22b are arrayed and which is disposed on the other side of the thin plate-shaped plate 22d. The electrode array section 22e is disposed in the thick tube 82b such that liquid sucked by the dispensing tip 82 can come into contact with the electrode 22a. The terminal array section 22f is disposed outside the thick tube 82b and is attachable to the plug 43a. Not that a reference numeral 42b at least includes a rod 42b through which a conductor passes. The conductive wire 22c is embedded in the plate 22d or a layered portion covered with or formed of an insulator.

(86) Subsequently, an operation in a case where the device for electrical measurement of a target chemical substance 102 and a method therefor according to Third Example of the present embodiment are applied to detection of a specific food allergen on the display obligation 7 items (egg, milk, wheat, buckwheat, peanut, shrimp, and a crab) for four kinds of foods will be described with reference to FIG. 13.

(87) The tip and the like storing section group 33a stores the electrode array elements 22.sub.1 to 22.sub.4 and the piercing tip with the attachment opening 82c facing upward such that the electrode array elements 22.sub.1 to 22.sub.4 and the piercing tip can be supported by being attached to the nozzles and the plugs 42a as the electrode array element support section or only to the nozzles.

(88) Two of the twelve electrodes of the electrode array elements 22.sub.1 to 22.sub.4 are electrodes for negative control and positive control. The electrode for negative control is blocked such that an antigen or an antibody is not bonded to the electrode. The electrode for positive control necessarily generates a predetermined electric signal. An antibody (for example, an anti-wheat antibody or an anti-egg antibody) capable of capturing an allergen is fixed to each electrode. Furthermore, each liquid storing section of the liquid storing section group 31b includes, in advance, in order, two sets of a liquid storing section storing 100 L of a food extract solution extracted from food and three liquid storing sections each storing 200 L of cleaning buffer solution (1PBS 0.05% Tween). Note that an antigen in each food extract solution is labeled in advance by bonding the antigen to an electrochemically active substance such as a metal complex via an amino group, a carboxyl group, or the like included in the antigen.

(89) In step S31, the processing head moving mechanism 52 moves the processing head 7 in the Y-axis direction, and moves the processing head 7 above a first storing section (electrode array element storing section) of the tip and the like storing section group 33a storing the electrode array elements 22.sub.1 to 22.sub.4. By lowering the four nozzles disposed in the processing head 7 by the Z-axis moving mechanism 53, attachment openings of the electrode array elements 22.sub.1 to 22.sub.4 are fitted with and attached to the nozzles. At the same time, the terminal array section 22f of the electrode array plate 22 is attached to each of the plugs 43.sub.1 to 43.sub.4.

(90) In step S32, the Z-axis moving mechanism 53 raises the nozzles 83a and the electrode array elements 22.sub.1 to 22.sub.4 in the Z-axis direction. Thereafter, the processing head moving mechanism 52 moves the nozzles 83a and the electrode array elements 22.sub.1 to 22.sub.4 in the Y-axis direction above the liquid storing section 33b storing the food extract solution. The Z-axis moving mechanism 53 lowers the electrode array elements 22.sub.1 to 22.sub.4 supported by the nozzles such that a mouth portion 82f at a tip end of each of the electrode array elements 22.sub.1 to 22.sub.4 is inserted into the food extract solution. The suction and discharge mechanism 83 repeatedly sucks and discharges the food extract solution. Suction is performed until reaching such a capacity that the electrode array section 22e of the electrode array plate 22 is immersed in the food extract solution to bring the electrodes 22a into contact with the food extract solution efficiently.

(91) In step S33, the Z-axis moving mechanism 53 raises the electrode array elements 22.sub.1 to 22.sub.4 in the Z-axis direction. Thereafter, the processing head moving mechanism 52 moves the electrode array elements 22.sub.1 to 22.sub.4 in the Y-axis direction above the liquid storing section 33b storing a cleaning liquid. The Z-axis moving mechanism 53 lowers the electrode array elements 22.sub.1 to 22.sub.4. By repeating suction and discharge by the suction and discharge mechanism 83, cleaning is performed. Similarly, cleaning is repeated with respect to other cleaning liquids.

(92) In step S34, the processing head moving mechanism 52 moves the electrode array elements 22.sub.1 to 22.sub.4 in the Y-axis direction above the measurement container 33c. The Z-axis moving mechanism 53 lowers the electrode array elements 22.sub.1 to 22.sub.4. Thereafter, the suction and discharge mechanism 83 sucks a solution stored in the measurement container 33c. While the mouth portion 82f is immersed in the solution, the applying units 62.sub.1 to 62.sub.4 apply a voltage to an antibody bonding to the electrode via the counter electrode 63, and the measurement sections 64.sub.1 to 64.sub.4 measure the amount of the voltage. The measurement result is digitally converted, is then stored in a storage unit in the CPU+program+memory 9, and is analyzed by the analysis unit 93.

(93) According to the device for electrical measurement of a target chemical substance and a method therefor according to the present Example, an electrode array element can be automatically attached to each nozzle (electrode array element support section). Therefore, contact with the electrode array element and a dispensing tip by a user can be prevented, and highly reliable processing without cross contamination can be performed. In addition, contact between each electrode of the electrode array element and liquid can be reliably performed by repeatedly sucking and discharging liquid. Therefore, efficiency and rapidity of the processing are high. Furthermore, all processing from liquid dispensing to measurement can be performed using an electrical mechanism. Therefore, it is not necessary to dispose an optical mechanism, and manufacturing can be performed compactly and easily, thereby inexpensively. Furthermore, according to the present Example, although the electrode array section is disposed so as to be able to come into contact with liquid sucked in the thick tube, the terminal array section and the plug are disposed outside the thick tube. Therefore, the terminal array section or the plug does not come into contact with the liquid, short circuit between electrodes can be prevented reliably, and highly reliable processing can be performed.

(94) The above-described embodiments have been described specifically for the purpose of better understanding of the present invention, and do not limit another embodiment. Therefore, the above-described embodiments can be changed within a range not changing the gist of the invention. For example, the above-described Examples have described that the processing head and the electrode array element support section move with respect to the container group. However, the container group may move while the processing head and the electrode array element support section are stationary.

(95) In addition, the numerical values, the number of times, the shape, the number, the amount, and the like used in the above description are not limited to these cases. For example, only the case where the cartridge containers of the container group, the electrode array elements, and the nozzles are arrayed in four rows has been described. However, it is needless to say that the present invention is not limited to this case. In addition, only the case where the number of electrodes or terminals of the electrode array element is 12 has been described. However, it is needless to say that the number of electrodes or terminals is not limited to this case.

(96) Furthermore, only the case where measurement is performed by performing labeling with an intercalating agent or a metal complex has been described. However, measurement can be performed by, for example, an alternating current impedance method without performing labeling.

(97) The devices described in Examples of the present invention, components forming these devices, devices for forming these components, reagents, and the like can be appropriately selected, can be appropriately modified, and can be mutually combined. Presence or absence of an antigen of each subject can be tested using, for example, an electrode array element, a dispensing element (dispensing tip and deformable dispensing tip), an electrode array element support section, a container group, a tip and the like storing section group, a cartridge container, a liquid storing section group, a measurement container or the like, a processing head, a support section moving mechanism, a measurement section, or the like. Alternatively, for example, in a case where a test in a serum solution is performed, presence or absence of an antigen of each subject can be tested by concentrating the serum solution using magnetic particles and fixing an antibody corresponding to each particle for use.

(98) In addition, for the electrode array element described in each of the above Examples, only the case where the electrodes and the terminals are arrayed on a plate-shaped base or a substrate has been described. However, it is needless to say that a case where electrodes and terminals are arrayed on an inner wall of a cylindrical or tubular base is included within the scope of the gist of the present invention.

(99) Incidentally, in the present application, a spatial expression such as X-axis, Y-axis, Z-axis, above, below, inside, outside, up and down, line, or row is only for illustrative purposes, and does not limit a particular spatial direction or arrangement of the structure.

INDUSTRIAL APPLICABILITY

(100) The present invention relates to a device for electrical measurement of a target chemical substance, and a method therefor, performs a test of a specimen collected from a patient or the like, and measurement thereof, and can be used particularly in a field requiring handling of a biopolymer such as a gene, an immune system, an amino acid, a protein, or a sugar, and a low molecular biological substance, for example, in various fields such a biochemistry field, an industrial field, an agriculture field such as food, agriculture, or fishery processing, a pharmaceutical field, and a medical field such as hygiene, health, immunity, diseases, or genetics.

REFERENCE SIGNS LIST

(101) 10, 101, 102 Device for electrical measurement of target chemical substance 2.sub.1 to 2.sub.n, 21.sub.1 to 21.sub.4, 22.sub.1 to 22.sub.4, 23.sub.1 to 23.sub.4 Electrode array element 21e, 22e, 23e Electrode array section 21a, 22a, 23a Electrode 21f, 22f, 23f Terminal array section 21b, 22b, 23b Terminal 21c, 22c, 23c Conductive wire 21d, 23d Base 22d Plate 22 Electrode array plate 3, 31, 32, 33 Container group 3.sub.1 to 3.sub.n Storing section group 31, 32.sub.1 to 32.sub.4, 33.sub.1 to 33.sub.4 Cartridge container 4, 41 Array element support 4.sub.1 to 4.sub.n, 41.sub.1 to 41.sub.4, 42.sub.1 to 42.sub.4, 43.sub.1 to 43.sub.4 Electrode array element support section 5(52, 53) Moving mechanism 7, 71, 72, 73 Processing head 8.sub.1 to 8.sub.n, 81.sub.1 to 81.sub.n Dispensing tip 82 Base (dispensing tip) 9 CPU+program+memory 15, 16, 151 to 156 Non-wetted electrode for application of external electric field