LOW TEMPERATURE INDICATOR MIXTURE, DEVICE AND METHOD FOR MONITORING A TEMPERATURE TRANSITION AT LOW TEMPERATURES

Abstract

The invention relates to a low temperature indicator mixture for indicating/monitoring a temperature transition within a temperature range of between 20 C. and 160 C., which comprises at least one dye, e.g. oil red, methyl rot, brilliant green, rhodamine B, and at least one alcohol selected from the group that comprises octane-1-ol, nonan-1-ol, propane-1,2-diol, propane-1,3-diol, butane-1,2-diol, butane-1,3-diol, butane-2-ol, pentane-1,5- diol, pentane-1-ol, cyclopentanol, benzyl alcohol, wherein the melt temperature of the mixture is within the aforementioned temperature range. Further aspects of the invention relate to devices and methods for indicating/monitoring a temperature transition within the aforementioned temperature range, in which devices and methods the aforementioned low temperature indicator mixture is used. In a specific embodiment, a device of this kind comprises at least the following components in at least one closed space: a) a partial region, which is at least partially filled with an indicator mixture as defined above; b) a second partial region, which is at least partially filled with an absorbent material, said material being optionally separated from the indicator mixture by a separating element; and c) optionally means to penetrate the separating element to bring the indicator mixture in a liquid state into contact with the absorbent material. In a method according to the invention for temperature monitoring using a device of this kind, a check is carried out to determine whether at least part of the indicator mixture was absorbed by the absorbent material, which indicates an at least temporary increase in the temperature of the device to at least the melt temperature of the indicator mixture.

Claims

1. A low temperature indicator mixture for indicating/monitoring a temperature transition within a temperature range from 20 C. to 160 C., which comprises: at least one dye; and at least one alcohol selected from the group consisting of octan-1-ol, nonan-1-ol, propane-1,2-diol, propane-1,3-diol, butane-1,2-diol, butane-1,3-diol, butan-2-ol, pentane-1,5-diol, pentan-1-ol, cyclopentanol, and benzyl alcohol; and wherein a melting temperature of the mixture lies within a temperature range from 20 C. to 160 C.

2. The low temperature indicator mixture according to claim 1, wherein the dye is selected from the group consisting of triphenylmethane dyes, rhodamine dyes, azo dyes, dyes and phenothiazine dyes.

3. The low temperature indicator mixture according to claim 1, wherein the dye is selected from the group consisting of oil red, methyl red, brilliant green, rhodamine B, neutral red, methylene blue.

4. The low temperature indicator mixture according to claim 1, wherein the alcohol is propane-1,3-diol, propane-1,2-diol or butan-2-ol.

5. The low temperature indicator mixture according to claim 1, wherein the concentration of the dye in the alcohol component is <10%, <1% or <0.1% of the saturation concentration.

6. The low temperature indicator mixture according claims 1-4, characterized in that it comprises or represents a solution of the dye in a concentration in the range of 10-100%, in particular 50-100% or 70-100% of the saturation concentration in the alcohol component.

7. The low temperature indicator mixture according to claim 1, comprising at least two different alcohol components: a) an alcohol selected from the group consisting of octan-1-ol, nonan-1-ol, propane-1,2-diol, propane-1,3-diol, butane-1,2-diol, butane-1,3-diol, butan-2-ol, pentane-1,5-diol, pentan-1-ol, cyclopentanol, and benzyl alcohol; b) an alcohol selected from the group consisting of octan-1-ol, nonan-1-ol, propane-1,2-diol, propane-1,3-diol, butane-1,2-diol, butane-1,3-diol, butan-2-ol, pentane-1,5-diol, pentan-1-ol, cyclopentanol, and benzyl alcohol with a lower melting point than the alcohol of component a); wherein a mixture ratio of components a) and b) is set so that a melting temperature of the mixture lies within a temperature range from 20 C. to 160 C., or from 25 C. to 160 C. or 50 C. to 150 C.

8. The low temperature indicator mixture according to claim 7, comprising one of the following combinations of components a) and b): octan-1-ol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; octan-1-ol and pentan-1-ol in a mixture ratio of 5 to 95 vol.-%; octan-1-ol and propane-1,2-diol in a mixture ratio of 5 to 95 vol.-%; nonan-1-ol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; nonan-1-ol and propane-1,2-diol in a mixture ratio of 5 to 95 vol.-%; nonan-1-ol and pentan-1-ol in a mixture ratio of 5 to 95 vol.-%; propane-1,2-diol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; propane-1,2-diol and propane-1,3-diol in a mixture ratio of 5 to 95 vol.-%; propane-1,2-diol and butane-1,2-diol in a mixture ratio of 5 to 95 vol.-%; propane-1,3-diol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; propane-1,3-diol and butane-1,2-diol in a mixture ratio of 5 to 95 vol.-%; pentane-1,5-diol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; benzyl alcohol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; pentan-1-ol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; pentan-1-ol and methanol in a mixture ratio of 5 to 95 vol.-%; cyclopentanol and butan-2-ol in a mixture ratio of 5 to 95 vol.-%; cyclopentanol and propane-1,2-diol in a mixture ratio of 5 to 95 vol.-%; cyclopentanol and pentan-1-ol in a mixture ratio of 5 to 95 vol.-%; or cyclopentanol and butane-1,2-diol in a mixture ratio of 5 to 95 vol.-%; wherein the mixture ratio relates in each case to a ratio of the former component in the mixture of both components.

9. The low temperature indicator mixture according to claim 7, wherein components a) and b) are selected from the group consisting of propane-1,3-diol, propane-1,2-diol and butan-2-ol and/or the dye is selected from the group consisting of oil red, methyl red, brilliant green and rhodamine B.

10. The low temperature indicator mixture according to claim 9, comprising propane-1,2-diol and butan-2-ol in a mixture ratio of 40 to 60 vol.-%, or propane-1,2-diol and propane-1,3-diol in a mixture ratio of 30 to 70 vol.-%, or propane-1,3-diol and butan-2-ol in a mixture ratio of 30 to 70 vol.-%.

11. The I ow temperature indicator mixture according to claim 1. wherein the liquid mixture in a temperature range of 3-30 C. or 5-30 C. above the melting temperature has a viscosity in a range from 10 to 10.sup.6 mPa.Math.s, or from prefably 10 to 10.sup.4 mPa.Math.s.

12-22. (canceled)

23. A device for indicating/monitoring a temperature transition within a temperature range from 20 C. to 160 C., 25 C. to 160 C. or 50 C. to 150 C., comprising at least the following components in at least one closed-off space: a) a first sub-region of the space which is filled at least partially with an indicator mixture according to claim 1; b) a second sub-region which is accessible for the indicator mixture in a liquid state; c) where applicable, a barrier which adjoins the first sub-region and which is located between the first sub-region and the second sub-region or in a portion of the second sub-region which adjoins the first sub-region, as well as, where applicable, means for breaking through the barrier.

24. The device according to claim 23, wherein: a the second sub-region which is at least partially filled with an absorbent material which is separated, where applicable, by a separating element from the indicator mixture; and further comprises where applicable, means for breaking through the separating element in order to bring the indicator mixture in the liquid state into contact with the absorbent material.

25. The device according to claim 23 forms a part of a cover, a base or a wall of a container for frozen material, in particular for a cryopreserved biological sample or represents this.

26. The device according to claim 24, wherein the absorbent material is selected from the group consisting of cellulose materials, plaster, chalk, porous membranes, fabrics, knitted fabrics, nano-porous aluminum oxide layers and microporous aluminumoxide layers.

27. A method for indicating/monitoring a temperature transition within a temperature range from 20 C. to 160 C., or from in particular 25 C. to 160 C. or 50 C. to 150 C., comprising a) providing an indicator mixture according to any one of claims 1 11 in a first sub-region of a closed-off space which comprises a first sub-region and a second sub-region, wherein the closed-off space also, where applicable, has a barrier, which adjoins the first sub-region, for the indicator mixture which is located between the first sub-region and the second sub-region or in a portion of the second sub-region which adjoins the first sub-region; b) cooled storage of the indicator mixture at a desired temperature within the above temperature range at which the indicator mixture is frozen; c) where applicable, causing the breaking through of a barrier between the first sub-region and the second sub-region or in a portion of the second sub-region which adjoins the first region after freezing of the indicator mixture; and d) checking whether at least a part of the indicator mixture is located in the second sub-region which indicates an at least temporary increase in the temperature to at least the melting temperature of the indicator mixture.

28. A method for indicating/monitoring a temperature transition within a temperature range from 20 C. to 160 C., or from 25 C. to 160 C. or 50 C. to 150 C., comprising a) providing a device according to claim 2 which comprises an absorbent material; b) cooled storage of the device at a desired temperature within the above temperature range at which the indicator mixture is frozen; c) bringing about the breaking through of a separating element between the indicator mixture and the absorbent material after freezing of the indicator mixture; and d) checking whether at least a part of the indicator mixture was absorbed by the absorbent material, which indicates an at least temporary increase in the temperature to at least the melting temperature of the indicator mixture.

29. The method according to claim 27, wherein the device comprises a barrier and/or an absorbent material and the presence of indicator mixture in the second sub-region of the closed-off space or in the absorbent material indicates that the indicator mixture was increased at least temporarily to a temperature above the melting temperature at which the viscosity of the melted indicator mixture undershoots a certain target value so that the melted indicator mixture can pass the barrier or be absorbed by the absorbent material.

30. The in method according to any one of claims 27, wherein the indicator mixture is selected so that its melting temperature or the temperature at which the viscosity of the melted indicator mixture undershoots a certain target value corresponds to a predetermined limit temperature, the exceeding of which is monitored.

31. The method according to claim 29, wherein the indicator mixture is selected so that its viscosity at the melting temperature or a predetermined limit temperature above the melting temperature, the exceeding of which is monitored, lies in a range which allows the absorption of liquid or transport of liquid through or into the absorbent.

32. The method according to claim 31, wherein the viscosity is in in a range from 10 to 1000 mPa.Math.s.

33. The low temperature indicator mixture according to claim 6, wherein the concentration of the dye in the alcohol component is set sufficiently high such that a melting point of the mixture is higher than the melting point of the mixture without dye by at least 10 C. or at least 20 C. or 30-70 C.

34. The low temperature indicator mixture according to claim 6, wherein the solution of the dye is a saturated solution of rhodamine B in butan-2-ol.

Description

[0137] A non-restrictive, illustrative example of a device according to the invention for indicating or monitoring a temperature transition is depicted in FIG. 1.

[0138] FIG. 1 illustrates in several cross-sectional views an exemplary embodiment 100 of a device for temperature monitoring. View A shows in this case an exploded representation of a cover 101 of a cryogenic tube 1 in which an apparatus for temperature monitoring is integrated. Device 100 comprises cryogenic tube 1 with a receiving space 2 for receiving a biological sample 6 as well as cover 101 which has a chamber 102 which contains an indicator mixture 7 which is separated from an absorbent material 103 by a separating element in the inactive state. Cover 101 is also referred to below as a temperature-sensitive (T-sensitive) cover. View B shows cover 101 in the mounted state, view C illustrates a cryogenic tube 1 which is ready for sale with a receiving space 2 for receiving a biological sample 6 and with screwed-on T-sensitive cover 101. Views A to C show device 100 in the as yet inactive state.

[0139] T-sensitive cover 101 comprises four parts: a screw insert 104, referred to below as screw-in part 104, an insert with liquid-absorbing structure 103, referred to below as absorbent material 103, which must be able to suck up liquid indicator mixture 7, a container 105 for the indicator mixture 7, embodied here by way of example as plastic pad 105, which contains dyed liquid as indicator mixture 7, if this is not frozen, and a base body 106 for screwing onto a commercially available cryogenic tube 1, embodied here as plastic cap 106.

[0140] In the inactive state of device 100, plastic pad 105 forms an impervious shell for indicator mixture 7 located therein; this shell forms a barrier which, in the inactive state, prevents indicator mixture 7 from coming into contact with absorbent material 103. The embodiment as a plastic pad has the advantage that it can be produced at very low cost.

[0141] Screw-in part 104 is embodied to be transparent or semi-transparent at at least one point so that at least a part of absorbent material 103 is visible from above through screw-in part 104. Screw-in part 104 can be produced, for example, from a transparent or semi-transparent material for this purpose. Base body 106 can furthermore be produced from a transparent or semi-transparent material for this purpose. In this manner, it is possible to check by means of a simple visual inspection from above whether the color state of absorbent material 103 has changed. This is the case e.g. if dyed indicator mixture 7 has penetrated into absorbent material 103 and as a result dyes it, which is further described below.

[0142] The base body or plastic cap 106 has an H-shaped cross-section, as a result of which two cylindrical cavities are formed. Upper cavity 102 forms a chamber with a first sub-region 102a in which container 105 with indicator substance 7 is located and with a second sub-region 102b in which absorbent material 103 is located.

[0143] The lower cavity serves to receive an upper end portion of cryogenic tube 1 in order to imperviously close it off. Cryogenic tube 1 is sealed off with a sealing ring 107. An engagement 4, e.g. a hexagonal hole via which screw-in part 104 can be screwed into base body 106 is located in screw-in part 103. Screw-in part 104 can also have blades 109 via which screw-in part 104 can also be screwed in. For the purpose of screwing in, there is provided on screw-in part 104 an external thread 104a which engages in a corresponding internal thread 106a of base body 106 which is provided on a side wall of upper cavity 102.

[0144] As already mentioned, views A to C show device 100 in the as yet inactive state. This means that plastic pad 105 filled with dyed indicator mixture is not destroyed and is impervious so that indicator mixture 7 in the liquid state cannot escape. Indicator mixture 7 in liquid form is initially located inside plastic pad 105. Absorbent material 103 located thereabove is not in contact with indicator mixture 7 as a result of intact plastic pad 105.

[0145] Screw-in part 104 is initially half screwed in (inactive state). In order to screw it further in, e.g. a quarter or half turn, a plastic stop in thread 103a, 106a must be broken through. It is thus ensured that the apparatus for temperature monitoring and/or the T-sensitive cover cannot be activated prior to use. Should this nevertheless occur prior to cryogenic storage of device 100 or sample 6, absorbent material 103 located in base body 106 is dyed and may consequently not be used. The transport and intermediate storage of cover 101 in the inactive state can be carried out over any desired period of time which promotes trade and stockpiling.

[0146] View D of FIG. 10 shows the activation of the device after the storage temperature is reached, in the present case e.g. <140 C.

[0147] Indicator mixture 7 is selected so that it is no longer liquid at the storage temperature in container 105, but rather is already solidified. Activation of the device is performed by screwing in screw-in part 104 in the case of frozen indicator mixture 7. Screw-in part 104 has at its lower side facing absorbent material 103 a protruding projection 108, e.g. in the form of a tip or a thorn. By screwing in screw-in part 104, projection 108 is bored into plastic pad 105 and destroys it. As a result, absorbent material 103 comes into direct contact with dyed indicator mixture 7. This is so viscous or even solid at the storage temperature that it is not drawn into absorbent material 103. The still ongoing thermal shocks are also not adequate for diffusion.

[0148] The device for temperature monitoring is now activated (active). As soon as a threshold value temperature which indicates the melting point of the mixture of indicator mixture during cryogenic storage is exceeded, frozen indicator mixture 7 becomes liquid. The viscosity drops with rising temperature until a threshold is exceeded from which absorbent material 103 takes in the liquid via capillary forces and thus takes on its color. This process is irreversible, i.e. even after subsequent renewed freezing of indicator mixture 7, the coloring of absorbent material 103 is maintained. If it is thus subsequently ascertained in the event of a visual inspection that absorbent material 103 has a coloring, it can be concluded that the melting temperature of the indicator mixture and moreover a slightly higher temperature than that of the melting temperature were also exceeded, at which indicator mixture 7 has become so viscous that it was taken in via capillary forces into absorbent material 103. The type and thickness of absorbent material 103 determines how quickly the apparent coloring occurs.

[0149] That which applies to absorbent material 103 and indicator mixture 7 inside base body 106 also applies to the temperature in biosample 6. An absorbent material which is dyed (e.g. red) thus indicates that biosample 6 has also at least temporarily exceeded the above-mentioned temperatures. Since this process can also not be reversed upon renewed deep-freezing, device 1 thus retains the information about impermissible heating. Although the invention has been described with reference to specific exemplary embodiments, it is apparent for a person skilled in the art that various changes can be made and equivalents can be used as a replacement without departing from the scope of the invention. The invention should consequently not be restricted to the disclosed exemplary embodiments, but rather should comprise all exemplary embodiments which fall into the scope of the enclosed claims. In particular, the invention also claims protection for the subject matter and the features of the subordinate claims independently of the claims referred to.