Use of stationary phase comprising fibril cellulose in separation methods

Abstract

Separation methods based on electrophoresis or chromatography that use a stationary phase including fibril cellulose.

Claims

1. A separation method comprising: separating two components by using a stationary phase comprising mechanically disintegrated nanofibrillar cellulose derived from plant material, wherein the stationary phase comprising mechanically disintegrated nanofibrillar cellulose is in the form of a hydrogel, the separation method being selected from methods based on electrophoresis or chromatography and the components being selected from synthetic polymers, natural polymers, macromolecules, biomolecules, nanoparticles, charged particles, charged ions, charged compound and combinations thereof.

2. The separation method according to claim 1, wherein the method based on electrophoresis is selected from gel electrophoresis, temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis, two-dimensional gel electrophoresis and DNA gel electrophoresis.

3. The separation method according to claim 1, wherein the method based on chromatography is selected from exclusion chromatography, ion exchange chromatography and affinity chromatography.

4. The separation method according to claim 1 wherein the amount of the nanofibrillar cellulose in the stationary phase is from 0.05 to 50 wt %.

5. A method for separating components selected from synthetic polymers, natural polymers, macromolecules, biomolecules, nanoparticles, charged particles, charged ions, charged compound and combinations thereof from a mixture, wherein said method comprises the steps of carrying out an electrophoresis method or chromatographic method where a stationary phase comprising mechanically disintegrated nanofibrillar cellulose derived from plant material is used, wherein the stationary phase comprising nanofibrillar cellulose is in the form of a hydrogel.

6. The method according to claim 5 wherein the electrophoresis method is selected from gel electrophoresis, temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis, two-dimensional gel electrophoresis and DNA gel electrophoresis.

7. The method according to claim 6 wherein the electrophoresis method comprises the steps of dispensing the mixture on or in the stationary phase, and applying an electrical field on the stationary phase whereby the components are separated.

8. The method according to claim 5 wherein the chromatographic method is selected from exclusion chromatography, ion exchange chromatography and affinity chromatography.

9. The method according to claim 8 wherein the chromatographic method comprises the steps of passing a mobile phase comprising at least one liquid through the stationary phase, where the mixture is dispersed in or on the stationary phase or it is mixed to the mobile phase, whereby the components are separated.

10. The method according to claim 9, wherein pressure or gravitational force is used.

11. The method of claim 1, further comprising forming the stationary phase from dry mechanically disintegrated nanofibrillar cellulose.

12. An electrophoresis kit for use in the method of claim 1, comprising stationary phase comprising mechanically disintegrated nanofibrillar cellulose derived from plant material, and reagents.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 depicts gel electrophoresis carried out with fibril cellulose gel matrix and agarose gel matrix. The gel was stained with ethidium bromide to make DNA visible under UV-light.

(2) FIG. 2 illustrates graphically quantitative PCR analysis results of extracted DNA samples from fibril cellulose gel after gel electrophoresis.

(3) FIG. 3 illustrates graphically results of B. cereus enumeration, said B. cereus cultured in two media, one containing 1.5 wt % of fibril cellulose (fc) and one without.

(4) FIG. 4 illustrates graphically quantitative PCR run from C. perfringens dilution series that show strong linear correlation between the dilution and the PCR response in the presence or absence of 1.5 wt % fibril cellulose.

(5) FIG. 5 depicts diffusion of different molecular weight dextrans (20 kDa, 70 kDa, and 250 kDa) through 1 wt % native fibril cellulose hydrogel.

DEFINITIONS

(6) Unless otherwise specified, the terms, which are used in the specification and claims, have the meanings commonly used in the field. Specifically, the following terms have the meanings indicated below.

(7) The term stationary phase refers here to an immobile phase or anticonvective medium and/or sieving medium.

(8) The term mobile phase refers here to liquid medium, which may comprise one or more component in liquid form. Typically the mobile phase is allowed to move through the stationary phase

(9) As used herein, the term fibril cellulose is understood to encompass all microfibrillated celluloses (MFC) and nanocelluloses. Further, there are several other widely used synonyms for fibril cellulose. For example: cellulose nanofiber, nanofibrillated cellulose (CNF), nanofibrillar cellulose (NFC), nano-scale fibrillated cellulose, microfibrillar cellulose, or cellulose microfibrils.

(10) Fibril cellulose produced by certain microbes has also various synonyms, for example, bacterial cellulose (BC), microbial cellulose (MC), biocellulose, nata de coco (NDC), or coco de nata (CDN).

DETAILED DESCRIPTION OF THE INVENTION

(11) It was surprisingly found that fibril cellulose can be used for providing a stationary phase particularly suitable for several separation methods. Fibril cellulose forms readily a stable gel in polar solvents and water. The properties of the gel may be varied according to the desired separation method and compounds to be separated or analyzed. Particularly the invention is directed to the use of a stationary phase comprising fibril cellulose in separation methods. Said separation methods are suitably based on electrophoresis or chromatography. Said chromatographic methods may suitably be selected from methods based on exclusion chromatography, ion exchange chromatography or affinity chromatography. Said methods are particularly useful in analyzing, separation and fractionation of natural polymers, synthetic polymers, macromolecules, biomolecules and nanoparticles and charged particles or compounds, including also preparative chromatographic methods. Electrophoresis includes here gel electrophoresis, temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis and two-dimensional gel electrophoresis and DNA gel electrophoresis.

(12) Exclusion chromatography includes size-exclusion chromatography, which may also be called as gel-filtration chromatography or gel-permeation chromatography.

(13) Ion exchange chromatography includes here anion exchange chromatography and cation exchange chromatography.

(14) Affinity chromatography includes here affinity chromatography, immunoaffinity chromatography and immobilized metal ion affinity chromatography.

(15) Fibril cellulose is obtained from any non-animal based cellulose raw material.

(16) The term cellulose raw material refers to any cellulose raw material source that can be used in production of cellulose pulp, refined pulp, and fibril cellulose. The cellulose raw material may be based on any plant material that contains cellulose or any microbial cellulose.

(17) Plant material may be wood and said wood can be from softwood tree such as spruce, pine, fir, larch, douglas-fir or hemlock, or from hardwood tree such as birch, aspen, poplar, alder, eucalyptus or acacia, or from a mixture of softwoods and hardwoods.

(18) Non-wood material can be from agricultural residues, grasses or other plant substances such as straw, leaves, bark, seeds, hulls, flowers, vegetables or fruits from cotton, corn, wheat, oat, rye, barley, rice, flax, hemp, manilla hemp, sisal hemp, jute, ramie, kenaf, bagasse, bamboo or reed.

(19) The cellulose raw material may be also derived from the cellulose-producing micro-organism, such as from bacterial fermentation processes. The micro-organisms can be of the genus Acetobacter, Agrobacterium, Rhizobium, Pseudomonas or Alcaligenes, preferably of the genus Acetobacter and more preferably of the species Acetobacter xylinum or Acetobacter pasteurianus.

(20) The term cellulose pulp refers to cellulose fibers, which are isolated from any cellulose raw material using chemical, mechanical, thermo-mechanical, or chemi-thermo-mechanical pulping processes. Cellulose pulp, which can be pulp of plant origin, especially wood (softwood or hardwood pulp, for example bleached birch pulp) and where the cellulose molecules are oxidized in one of the above-described methods, is easy to disintegrate to fibril cellulose.

(21) The term fibril cellulose refers to a collection of isolated cellulose microfibrils (nanofibers) or microfibril bundles derived from cellulose raw material. Microfibrils have typically high aspect ratio: the length exceeds one micrometer while the number-average diameter is typically below 200 nm. The diameter of microfibril bundles can also be larger but generally less than 1 m. The smallest microfibrils are similar to so called elementary fibrils, which are typically 2-12 nm in diameter. The dimensions of the fibrils or fibril bundles are dependent on raw material and disintegration method.

(22) Fibril cellulose is characterized by very high water retention values, a high degree of chemical accessibility and the ability to form stable gels in water or other polar solvents. Fibril cellulose product is typically a dense network of highly fibrillated celluloses. The fibril cellulose may also contain some hemicelluloses; the amount is dependent on the plant source.

(23) To obtain fibril cellulose mechanical disintegration of cellulose pulp, oxidized cellulose raw material or microbial cellulose is carried out with suitable equipment such as a refiner, grinder, homogenizer, colloider, friction grinder, ultrasound-sonicator, fluidizer such as microfluidizer, macrofluidizer or fluidizer-type homogenizer. Preferably mechanically disintegrated fibril cellulose is used.

(24) Several different grades of fibril celluloses have been developed using various production techniques. The grades have different properties depending on the manufacturing method, degree of fibrillation and chemical composition. The chemical compositions of the grades also vary. Depending on the raw material source, e.g. HW vs. SW pulp, different polysaccharide composition exists in the final fibril cellulose product. Typically, non-ionic or native or neutral grades have wider fibril diameter while the chemically modified grades are a lot thinner. Size distribution is also narrower for the modified grades.

(25) The fibril cellulose refers here to one grade of fibril cellulose or a combination of two or more different grades of fibril cellulose. For example modified grades of fibril cellulose may be blended with native grade for enhancing binding of certain compounds to the gel or binding of some specific impurities etc.

(26) According to one embodiment of the invention plant derived native fibril cellulose may be used, suitably as a gel, such as a hydrogel (aqueous gel).

(27) Fibril cellulose is understood to encompass here also any chemically or physically modified derivates of cellulose, cellulose nanofibers or nanofiber bundles, obtained from any cellulose raw materials. The chemical modification may be based for example on carboxymethylation, oxidation, (TEMPO-oxidation), esterification, or etherification reaction of cellulose molecules, whereby aninically or cationically modified grades are obtained. Modification may also be realized by physical adsorption of anionic, cationic, or non-ionic substances or any combination of these on cellulose surface. The described modification can be carried out before, after, or during the production of cellulose nanofibers. Certain modifications may lead to materials that are degradable in human body. Modified grades are typically prepared from bleached pulps. In the modified grades, the hemicelluloses are also modified together with the cellulose domain. Most probably, the modification is not homogeneous, i.e. some parts are more modified than others. Thus, detailed chemical analysis is not possible-the modified products are always complicated mixtures of different polysaccharide structures.

(28) Chemically modified grades, such as anionic and cationic grades typically have their surface charge modified and they may suitably be used as dry powder or an aqueous gel. Chemically modified grades may be used particularly in the separation of compounds, where a specific surface charge enhances the separation. Thus suitable fibril cellulose or a combination of different fibril celluloses may de selected and designed for this purpose according to the type of the compounds to be separated. Dry powders of fibril cellulose may conveniently be manufactured by spray drying and/or lyophilization of suspension or dispersions containing said fibril cellulose, using any conventional methods known in the art. Suitably the chemically modified grades are spray dried and optionally granulated to small beads. These may be reconstituted into gel with a polar solvent, suitably water.

(29) The fibril cellulose gel or hydrogel refers here to a dispersion of fibril cellulose. The fibril cellulose has excellent gelling ability, which means that it forms a hydrogel already at a low consistency in an aqueous medium.

(30) Suitably the cellulose raw material such as cellulose pulp is pretreated with acid and base prior to the mechanical disintegration. The pretreatment is effected by subjecting the cellulose pulp to acid treatment, preferably with hydrochloric acid for removing any positively charged ions having a charge more than +1, followed by treatment with an inorganic base containing positively charged ions having a charge +1, preferably NaOH, where Na.sup.+ ions replace the earlier ions. The absence of any positively charged ions having a charge more than +1 is particularly advantageous in life science and molecular biology applications where complex formation of DNA with ions with charges more than +1 can be avoided. The pretreatment provides the final product excellent gelling properties and transparency. The fibril cellulose obtained from pretreated cellulose raw material is referred to here as ion exchanged fibril cellulose. According to one embodiment of the invention ion exchanged fibril cellulose is used.

(31) Microbial purity of fibril cellulose is often essential. Therefore, fibril cellulose may be sterilized prior to use, suitably in a gel form. In addition, it is important to minimize the microbial contamination of the product before and during the mechanical disintegration, such as fibrillation. Prior to fibrillation/mechanical disintegration, it is advantageous to aseptically collect the cellulose pulp from the pulp mill immediately after bleaching stage when the pulp is still sterile.

(32) Fibril cellulose hydrogels have typically remarkable high yield stress and high zero-shear viscosity at low concentrations. Thus, i.e. if gas bubbles are generated in the fibril cellulose hydrogels they may stay still for long periods of time. The buoyancy of gas bubbles can be, however, easily increased by lowering gas pressure (e.g. 15 mmHg) above the gel, which lowers the solubility of gas in the hydrogels phase and, respectively increases the volumes of initial gas bubbles. The increased gas bubbles escape easily to upper gas phase.

(33) The stationary phase comprising fibril cellulose may comprise one grade of fibril cellulose or a combination of different grades, such as native and modified grades (anionic grade, cationic grade etc.).

(34) Modified grades and native or non-ionic grades may be used in gel electrophoresis methods, however native grades are particularly suitable.

(35) In exclusion chromatographic and affinity chromatographic methods the selection of the fibril cellulose grade depends on the compounds, which are analyzed or separated. In size exclusion chromatography suitably native or non-ionic grade of fibril cellulose is used.

(36) In ion-exchange chromatography the modified grades, such as anionic or cationic grades may be suitable. The modified grades may be regenerated after the separation or analysis in a similar manner as conventional ion-exchange resins, followed by reuse.

(37) The stationary phase may comprise the fibril cellulose as a gel, preferably a hydrogel, or alternatively as a dry powder, which may be reconstituted prior use to a gel by bringing it in contact with a polar solvent or a mixture thereof, suitably water whereby a hydrogel is obtained. Particularly suitably the modified grades, such as anionic and cationic grades, are provided as dry powder. The native and non-ionic grades are suitably provided as gels, such as hydrogels.

(38) The number average fibril diameter of the fibril cellulose in a stationary phase is selected according to the requirements of the separation method, thus a suitable stationary phase may be designed for each specific method, taking into account for example the chromatographic requirements, desired resolution, properties of the compounds which are separated etc. The number average fibril diameter of the fibril cellulose may range from 1 to 1000 nm, suitably from 1 to 200 nm, and according to one embodiment the number average fibril diameter of native grades is from 1 to 100 nm, and in chemically modified grades from 1 to 20 nm.

(39) The stationary phase may comprise 0.05-100 wt % of at least one grade of fibril cellulose. The stationary phase may optionally comprise additives.

(40) Suitably, in gel electrophoresis, a gel comprising 0.05-20 wt %, suitably 0.1-3 wt % and according to one embodiment 0.5-1.5 wt % of fibril cellulose is used as stationary phase. Suitably the fibril cellulose is native or non-ionic fibril cellulose. The stationary phase may optionally comprise additives, such as dyes, markers, activated carbon etc. The gel may be a ready-to-use casted gel, which may additionally be sterilized and packed in sterile packages, or it may be packed as a hydrogel in a syringe, which can be used for the application of the gel. The gel may also be used in combination with conventional gels, such as agarose or polyacrylamide gels, for example as sections in said gels. The gel may also be in the form of a dry membrane which can be reconstituted with water prior to use. Anionic grades, such as carboxymethylated fibril cellulose may be used for dry membranes.

(41) After running the gel electrophoresis, separated compounds can easily be removed for example by pipetting, followed by suitable rework, purification etc. The stationary phase comprising fibril cellulose may be used in any gel electrophoresis applications known in the art. Particularly suitably the stationary phase comprising fibril cellulose may be used in application in the field of forensic, molecular biology, genetics, microbiology and biochemistry.

(42) Further, the stationary phase comprising fibril cellulose may be incorporated in a gel electrophoresis kit, which comprises reagents necessary for carrying out the gel electrophoresis and the stationary phase comprising fibril cellulose as precasted gel or provided as hydrogel, suitably in a dispenser such as in a syringe.

(43) In exclusion chromatography dry powder or beads or a gel comprising 0.05-50 wt %, suitably 1-30 wt % and according to one embodiment 2-20 wt % of fibril cellulose may be used as the stationary phase. The stationary phase in the form of dry powder or beads may be reconstituted prior use to a gel by bringing it in contact with a polar solvent or a mixture thereof, suitably water. The stationary phase may optionally comprise additives, such as dyes, markers, activated carbon etc. The dry powder or beads, or the hydrogel comprising the fibril cellulose may easily be packed in a vessel, such as a column, having at least one filter arranged therein, preferably at the other end (bottom) of the column for keeping the fibril cellulose therein. Accordingly ready-to-use prepacked columns, which may also be sterilized, may be provided to end users. The stationary phase comprising fibril cellulose may be used in any exclusion chromatography applications known in the art.

(44) In ion exchange chromatography dry powder or beads or a gel comprising 0.05-50 wt %, suitably 1-30 wt % and according to one embodiment 2-20 wt % of fibril cellulose may be used as stationary phase. The dry powder or beads may be reconstituted prior use to a gel by bringing it in contact with a polar solvent or a mixture thereof, preferably water. The stationary phase may optionally comprise additives, such as dyes, markers, activated carbon etc. The dry powder or beads, or the hydrogel comprising the fibril cellulose may easily be packed in a vessel, such as a column, having at least one filter arranged therein, preferably at the other end (bottom) of the column for keeping the fibril cellulose therein. Accordingly ready-to-use prepacked columns, which may also be sterilized, may be provided to end users. The stationary phase comprising fibril cellulose may be used in any ion exchange chromatography applications known in the art, including large scale applications for the separation of charged particles, charged ion or charged compounds from mixtures.

(45) In applications based on affinity chromatography dry powder or beads or elements or a gel comprising 0.05-50 wt %, suitably 1-30 wt % and according to one embodiment 2-20 wt % of fibril cellulose may be used as the stationary phase. The stationary phase in the form of dry powder or beads or elements may be reconstituted prior use to a gel by bringing it in contact with a polar solvent or a mixture thereof, suitably water. The stationary phase may optionally comprise additives, such as dyes, markers, activated carbon etc. The dry powder or beads or the hydrogel comprising the fibril cellulose may easily be packed in a vessel, such as a column, having at least one filter arranged therein, preferably at the other end (bottom) of the column for keeping the fibril cellulose therein. Accordingly ready-to-use prepacked columns, which may also be sterilized, may be provided to end users. The stationary phase comprising fibril cellulose may be used in any affinity chromatography applications known in the art.

(46) The elements may be obtained by casting the gel and drying it or by extrusion of a fibril cellulose gel into an organic solvent.

(47) The hydrogel comprising fibril cellulose can very easily be dispersed, or injected in a column for example with a syringe.

(48) The stationary phase comprising fibril cellulose may suitably also be used in separation methods on a larger industrial scale, in process scale applications, particularly in the field of dairy industry for separation of proteins, in the separation and fractionation of carbohydrates and other synthetic and natural polymers, biomolecules, macromolecules and nanoparticles, and in purification methods of liquids and mixtures, where separation of charged particles, charged ions such as metal ions or charged compounds is desired.

(49) The used stationary phase, containing for example protein or carbohydrate fractions or residues may easily be taken out from the process equipment for example by draining and it may conducted for further use, such as a component in animal feeds, particularly in the case non-pathogenic and non-toxic ingredients were used in the separation method.

(50) The working range pH of the stationary phase may be adjusted by selecting a suitably modified grade of fibril cellulose.

(51) The diffusion rate may be adjusted by selecting a suitable chemically modified fibril cellulose grade or a combination of fibril celluloses, suitable number average fibril size range of the fibril cellulose, suitable force which is used for driving the mobile phase through the stationary phase, for example pressure, gravity, electromotive force, etc.

(52) The use of stationary phase comprising fibril cellulose in separation methods based on gel electrophoresis or exclusion chromatography or ion exchange chromatography provides several valuable effects. As fibril cellulose is not a polymerization product, there are no monomer residues, such as neurotoxic acrylamide, left in the product. With regard to nucleic acid analysis and isolation, the risk of potential enumeration and detection problems can be avoided or at least significantly reduced by the use of a stationary phase comprising fibril cellulose in the separation methods.

(53) The stationary phase comprising fibril cellulose is a nontoxic product, which is easy to manufacture, easy to handle and requires no specific precautions from the end user. Suitably the hydrogel may be injected to column, injected or casted or spread on plates and wells and it may be dispersed or pipetted. As can be seen from the examples it has no adverse effects and does not interfere with DNA isolation or PCR analysis, and in fact the use of fibril cellulose improves DNA isolation yields.

(54) The material remaining in the stationary phase after separation may easily be removed or isolated by diluting with water, followed by decanting or centrifuging, or alternatively the fibril cellulose may be enzymatically degraded with for example cellulose enzymes.

(55) In the case of gel electrophoresis, the bands containing the desired components obtained after the separation procedure can easily and exactly be drawn with a syringe and the components can be isolated from the hydrogel.

(56) The following examples are illustrative of embodiments of the present invention, as described above, and they are not meant to limit the invention in any way.

EXAMPLES

(57) Fibril cellulose samples: Native fibril cellulose was produced by high pressure homogenization (five subsequent cycles) of highly purified bleached birch pulp, followed by autoclave sterilization. After fluidization, the fibril cellulose was dilute hydrogel (1.8 wt %). Ion-exchanged native fibril cellulose was obtained in a similar manner but additionally prior to fibrillation it was subjected to acid-base treatment in order to remove high valency cations (method described in previous sections). After high pressure homogenization (15 subsequent cycles) the ion-exchanged fibril cellulose forms a strong hydrogel having lower turbidity compared to the other sample. Fibril cellulose was sterilized by autoclaving when necessary. Transparent anionic fibril cellulose was obtained as hydrogel (0.9 wt %) by similar homogenization process of a chemically modified cellulose pulp (TEMPO-oxidized cellulose pulp).

Example 1

Use of Electrophoresis Gel Matrix Based on Fibril Cellulose

(58) Agarose gel containing 1.5% by weight of agarose was prepared according normal laboratory practice, after which two about 3 cm5 cm slices of the gel were cut and removed from the central part of the gel. These two holes were filled with fibril cellulose hydrogel containing 1.5% by weight of native fibril cellulose and the whole gel system was immersed in 1TAE buffer for electrophoresis. The selection of the fibril cellulose type and grade and mixing of the fibril cellulose hydrogel have impact on the turbidity, appearance and separation capability of the hydrogel. Preferably the mixing is carried out carefully in order to avoid any air bubbles in the gel, which may cause increased turbidity of the gel.

(59) The gel was loaded with complete 16S gene E. coli gene (estimated MW about 1.4 kb) and taxonomically important smaller C. perfringens S16 fragment (estimated molecular weight about 0.6 kb). The electrophoresis was carried out and the gel was stained with ethidium bromide that makes DNA visible under UV-light. (A) the whole 16S gene, (B) 16S fragment and (C) dimer of 16S fragment (i.e. dimer of 16S fragment). The ladders are molecular weight markers.

(60) The smaller 16S fragment contains two bands, the smaller is the actual fragment and the higher molecular weight fragment is its dimer. The gel was run at 120 volt until the ethidium bromide stained DNA fragments were clearly migrated inside the fibril cellulose matrix, after which the gel run was stopped and photographed, see FIG. 1.

(61) The stained gel demonstrates that the fibril cellulose matrix acts in a similar way as agarose and it is able to separate the DNA fragments based on their molecular weight. The DNA bands are little fainter in fibril cellulose, which is likely caused by lower transparency of the used fibril cellulose material. Furthermore, the migration rate is higher in fibril cellulose gel, which means that similar migration rate can be achieved by using more concentrated gel mixture.

(62) Next procedure was to isolate the DNA fragments from the gel. In agarose it requires mechanical cutting of gel material. In fibril cellulose the DNA fragments were simply pipetted out from the gel. This is clearly faster and easier. Thereafter quantitative PCR analysis of extracted DNA samples from fibril cellulose gel was carried out using universal primers and Sybergreen I chemistry as described above, see FIG. 2. The data indicates that almost same signal was gained from line 4 and 9 and from line 5 and 10, which show good reproducibility for extracted DNA fragments.

Example 2

Enumeration of Microorganisms from Fibril Cellulose Carrier

(63) Bacillus cereus bacterium was cultivated in two different media, one containing 1.5 wt % of native fibril cellulose and one without it, and it was measured if the fibril cellulose was able to provide higher microbial cell numbers. PCR based enumeration was used in the trial and it provided excellent example of fibril cellulose's interaction with the PCR detection.

(64) The growth medium used in the experiment was standard trypsin soy broth, and it was prepared according the manufacturer's instructions and autoclaved for sterility. Before autoclaving the growth medium was divided into the two parts, one of which 1.5 wt % of fibril cellulose was added. Small inoculum of B. cereus was grown overnight at 37 C. to reach dense microbial culture, and that was used to inoculate both growth media, about thousand fold dilution was used in the inoculation. Both test media were sampled 0 and 24 hours after the inoculation. 0.1 ml sample of culture was used for PCR based microbial enumeration. The enumeration method was based on the Ruminolyze protocol, where the microbial sample is first diluted to washing buffer, and microbial cells are pelleted by centrifugation (10 min 18 000g). The supernatant is discarded and pellet is suspended to enzymatic lysis buffer and thereafter strongly beaten with glass beads in order to both enzymatically and mechanically lyse microbial cells to release their DNA content. The released DNA is purified with phenol-chloroform extraction, then precipitated with ethanol and finally dissolved to the DNA storage buffer. The B. cereus enumeration was performed with two B. cereus selective DNA primers and Sybergreen I chemistry. The results are shown in FIG. 3.

(65) Accordingly Bacillus cereus cell numbers on growth media (TSB) and fibril cellulose (fc) in the beginning (0 h) and after overnight incubation were analyzed by quantitative PCR. The experiment indicates that small number of B. cereus cells, about 10.sup.5 cells/ml, can be detected in the presence of fibril cellulose that indicates that fibril cellulose has no effect on the sensitivity of the PCR assay. Furthermore, 24 hour sample gives higher cell numbers than the 0 hour sample. This means that the dynamic range of PCR is not disturbed by the presence of fibril cellulose and the fibril cellulose does not hinder the PCR reaction when is it run at its maximum speed, in other words when there are large amounts of template DNA. FIG. 3 also shows that more cells are counted in the presence of fibril cellulose at both 0 hours and 24 hours sampling points. This suggests that fibril cellulose aids the DNA isolation and provides higher DNA yields for B. cereus enumerations. This also suggests that fibril cellulose even improves PCR protocols.

(66) Similar experiments were also performed with other bacterial species, Clostridium perfringens, Lactobacillus salivarius and Desulfovibrio desulficans. The results were similar to B. cereus findings, and they support the contention that the fibril cellulose does not inhibit DNA isolation or PCR procedures.

Example 3

Microbial Quantification in the Presence of Fibril Cellulose

(67) Dilution series (10-, 100 and 1000-fold dilution) of dense Clostridium perfringens culture (about 10.sup.9 cells/ml) was prepared in the presence of 1.5 wt % of native fibril cellulose and without it. The diluted microbial samples were suspended to lysis buffer and glass beads and exposed to microbial DNA isolation method (as described in example 2 above). After the DNA isolation the DNA samples were used for PCR based enumeration by using C. perfringens specific primers and Sybergreen I chemistry. The results of PCR quantification are shown graphically in FIG. 4. The data shows strong linear correlation between PCR results and calculated dilution without any interference from fibril cellulose, i.e. between the dilution and the PCR response in the presence or absence of 1.5 wt % of fibril cellulose.

Example 4

Diffusion of Dextrans through Fibril Cellulose Hydrogels

(68) Diffusion properties of fibril cellulose hydrogel were demonstrated with different molecular weight dextrans in the following manner:

(69) 400 l of transparent or opaque fibril cellulose hydrogel (1 wt % native fibril cellulose) was planted per filter on the apical compartment in Transwell filter well plates (filter pore size 0.4 m). 1 ml of PBS was added into the basolateral side and 100 l (25 g) of fluorescent labeled dextrans were added on top of the hydrogels (MW of 20 k, 70 k and 250 k). Plate was fixed firmly and left undisturbed on a well plate rocker. 100 l samples were taken from the basolateral side and equal amount was replaced with PBS. First samples were taken with 15 minute intervals, other samples were taken with different time points ranging from 30 minutes to 2 hours and final samples at 24 hours. Total of 168 samples were taken. Target plate (OptiPlate-96 F) was measured at excitation and emission wavelengths 490 nm and 520 nm respectively.

(70) Diffusion of different molecular weight dextrans through 1% native fibril cellulose gel is presented in FIG. 5. The diffusion of the model compounds takes place at constant rate and it is highly dependent on the molecular weight (size) of the compound. It is clear that in the CNF hydrogels molecules are able to diffuse efficiently although the gel structure is firm enough to stabilize the cell suspension.

(71) The diffusion of compounds can be controlled as a function of the size of the molecule (protein) or as a hydrogel concentration.

(72) While the invention has been described with respect to specific examples including presently preferred modes of carrying out the invention, those skilled in the art will appreciate that there are numerous variations and permutations of the above described embodiments that fall within the spirit and scope of the invention. It should be understood that the invention is not limited in its application to the details of construction and arrangements of the components set forth herein. Variations and modifications of the foregoing are within the scope of the present invention.