TARGET MATERIAL EXTRACTION KIT FOR CENTRIFUGAL SEPARATOR, AND METHOD FOR EXTRACTING TARGET MATERIAL BY USING SAME
20230012405 · 2023-01-12
Assignee
Inventors
Cpc classification
B01L3/5021
PERFORMING OPERATIONS; TRANSPORTING
A61J1/05
HUMAN NECESSITIES
International classification
B01L3/00
PERFORMING OPERATIONS; TRANSPORTING
A61J1/05
HUMAN NECESSITIES
B04B11/00
PERFORMING OPERATIONS; TRANSPORTING
Abstract
The present invention relates to a target material extraction kit for a centrifugal separator and a method for extracting a target material by using the target material extraction kit for a centrifugal separator, and more specifically, to a target material extraction kit for a centrifugal separator and a method for extracting a target material by using the target material extraction kit for a centrifugal separator by which the target material can be more easily extracted from a source material that is separated into layers by the centrifugal separator, and thus simplification of the extraction operation of the target material results in improvement in easiness and efficiency of the extraction operation.
Claims
1. (canceled)
2. (canceled)
3. (canceled)
4. A method for extracting a target material by using a target material extraction kit for a centrifugal separator, the method comprising: a source material collecting step (S100) of collecting a source material of a subject by using a source material collecting means (100); and a target material extracting step (S200) of extracting the target material from the source material separated by the centrifugal separator by using a target material extracting means (200) after the source material collecting step (S100), wherein the source material collecting step (S100) is configured to include: a collecting means preparing step (S110) of preparing the source material collecting means (100) so as to collect the source material from the subject; and a source material filling step (S120) of collecting the source material from the subject by using the source material collecting means (100), wherein the target material extracting step (S200) is configured to include: an extracting means preparing step (S210) of preparing the target material extracting means (200); an extracting means coupling step (S220) of coupling the target material extracting means (200) to the source material collecting means (100) filled with the source material collected from the subject; a centrifugation preparing step (S230) of moving the source material, with which the source material collecting means (100) is filled, into the target material extracting means (200) coupled to the source material collecting means (100) by applying pressure to the source material collecting plunger unit (120); a plunger element decoupling step (S240) of decoupling a plunger element (122) from the source material collecting barrel unit (110) of the source material collecting means (100) before the source material collecting means is inserted into the centrifugal separator; a centrifuging step (S250) of separating a source material through centrifugation by inserting the source material collecting means (100), from which the source material collecting plunger unit (120) is decoupled, and the target material extracting means (200) into the centrifugal separator; a plunger element coupling step (S260) of coupling and fixing the plunger element (122) to a target material discharging plunger unit (220) of the target material extracting means (200) filled with the source material separated into layers by the centrifugal separator; a primary target material extracting step (S270) of injecting a part of the source material separated into layers, with which a target material containing barrel unit (210) is filled, into the source material collecting barrel unit (110) by applying pressure to the plunger element (122) coupled and fixed to the target material discharging plunger unit (220) to move the target material discharging plunger unit forward; a syringe body coupling step (S280) of decoupling the source material collecting means (100) from the target material extracting means (200) after the primary target material extracting step (S270) and coupling and fixing a syringe body which is to be filled with a target material, to the target material extracting means (200); a secondary target material extracting step (S290) of injecting a part of the source material separated into layers, which remains in the target material containing barrel unit (210), into the syringe body by re-applying pressure to the plunger element (122) coupled and fixed to the target material discharging plunger unit (220) to move the target material discharging plunger unit forward; and a target material preparation finishing step (S300) of decoupling the syringe body from the target material extracting means (200) and finishing a target material extracting operation, thereby the target material can be easily extracted from the source material.
Description
BRIEF DESCRIPTION OF DRAWINGS
[0042] The above and other objects, features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which:
[0043]
[0044]
[0045]
[0046]
[0047]
[0048]
REFERENCE CHARACTER LIST
[0049] 1: target material extraction kit for a centrifugal separator
[0050] 2: method for extracting a target material by using the target material extraction kit for a centrifugal separator
[0051] 100: source material collecting means
[0052] 110: source material collecting barrel unit
[0053] 111: source material collecting space module
[0054] 111a: first slope surface
[0055] 112: target material extracting means coupling module
[0056] 112a: source material passage projecting element
[0057] 112b: step element
[0058] 112c: source material flowing passage element
[0059] 113: first barrel handle
[0060] 114: first airtight projection element
[0061] 120: source material collecting plunger unit
[0062] 121: first airtight packing element
[0063] 122: plunger element
[0064] 123: first airtight packing-plunger connector element
[0065] 200: target material extracting means
[0066] 210: target material containing barrel unit
[0067] 211: target material containing space module
[0068] 211a: second slope surface
[0069] 212: source material collecting means coupling module
[0070] 212a: target material passage projecting element
[0071] 212b: target material flowing passage element
[0072] 212c: leakage preventive space element
[0073] 213: plunger fine adjustment zone module
[0074] 214: second airtight projection element
[0075] 220: target material discharging plunger unit
[0076] 221: second airtight packing element
[0077] 222: second airtight packing-plunger connector element
[0078] S100: source material collecting step
[0079] S110: collecting means preparing step
[0080] S120: source material filling step
[0081] S200: target material extracting step
[0082] S210: extracting means preparing step
[0083] S220: extracting means coupling step
[0084] S230: centrifugation preparing step
[0085] S240: plunger element decoupling step
[0086] S250: centrifuging step
[0087] S260: plunger element coupling step
[0088] S270: primary target material extracting step
[0089] S280: syringe body coupling step
[0090] S290: secondary target material extracting step
[0091] S300: target material preparation finishing step
[0092] SY: syringe body
[0093] a.sub.1: angle of first slope surface (111a)
[0094] a.sub.2: angle of second slope surface (211a)
[0095] 122′: target material extracting operation-dedicated plunger element
BEST MODE
Mode for Invention
[0096] Hereinafter, functions, configurations, and operations of a target material extraction kit (1) for a centrifugal separator and a method (2) for extracting a target material by using the same of the present invention will be described in detail with reference to the accompanying drawings.
[0097]
[0098] According to the present invention, as illustrated in
[0099] The target material is to be easily separated and extracted from the source material separated into the layers formed inside the source material collecting means (100) and the target material extracting means (200) by the centrifugal separator.
[0100] In other words, the present invention relates to the target material extraction kit (1) for a centrifugal separator which enables a target material to be extracted from a source material even through centrifugation performed only once by using the source material collecting means (100) and the target material extracting means (200) and enables an extraction operation to be hygienically performed in terms of an extraction operation environment, thereby resulting in not only easiness of the extraction operation but also improvement in the extraction operation environment.
[0101] More specifically, a first embodiment is described as follows. The source material collecting means (100) that is a means for collecting a certain amount of source material including a target material from a subject is configured to include: a source material collecting barrel unit (110) which is to be filled with the certain amount of source material; and a source material collecting plunger unit (120) which is inserted into the source material collecting barrel unit (110), is moved forward and backward, and generates pressure by moving forward and backward to fill the source material collecting barrel unit (110) with the certain amount of source material.
[0102] The source material collecting barrel unit (110) is configured to include: a source material collecting space module (111) in which the certain amount of source material is stored; and a target material extracting means coupling module (112) which is formed to project from one end portion of the source material collecting space module (111) such that a part of the target material extracting means coupling module is inserted into and coupled to the target material extracting means (200).
[0103] The source material collecting plunger unit (120) is configured to include: a first airtight packing element (121) which seals an inside of the source material collecting barrel unit (110) to form the airtight inside; a plunger element (122) which moves the first airtight packing element (121) forward and backward inside the source material collecting barrel unit (110); and a first airtight packing-plunger connector element (123) that has one end portion to which the first airtight packing element (121) is coupled and fixed and the other end portion to which the plunger element (122) is coupled and fixed such that the first airtight packing element (121) is moved forward and backward simultaneously along with forward and backward movement of the plunger element (122).
[0104] The target material extracting means coupling module (112) is configured to include: a source material passage projecting element (112a) which extends and projects from the source material collecting space module (111) by a certain length and is inserted into and fixed to the target material extracting means (200) so as to be coupled to the target material extracting means (200); a step element (112b) which is provided to form a step by being positioned between the source material passage projecting element (112a) and the source material collecting space module (111) and having a diameter larger than an outer diameter of the source material passage projecting element (112a); and a source material flowing passage element (112c) which penetrates the source material passage projecting element (112a) and is connected to the source material collecting space module (111) so as to enable a source material to flow into the source material collecting space module (111).
[0105] Accordingly, the source material can be easily collected from a subject.
[0106] In this case, the target material extracting means (200) that is coupled and fixed to one side of the source material collecting means (100) filled with the source material collected from the subject and is inserted into the centrifugal separator is configured to include: a target material containing barrel unit (210) in which the source material including a target material separated by the centrifugal separator is collected; and a target material discharging plunger unit (220) which is inserted into the target material containing barrel unit (210) to form the airtight target material containing barrel unit and is moved forward and backward depending on an operation of an operator to generate pressure inside the target material containing barrel unit (210).
[0107] The target material containing barrel unit (210) is configured to include: a target material containing space module (211) in which the source material including the target material separated by the centrifugal separator is stored; and a source material collecting means coupling module (212) which is formed to project from an end portion of the target material containing space module (211) such that an end portion of the source material collecting means (100) is inserted into and coupled to the source material collecting means coupling module.
[0108] The target material discharging plunger unit (220) is configured to include: a second airtight packing element (221) which seals an inside of the target material containing barrel unit (210) to form the airtight inside; and a second airtight packing-plunger connector element (222) that has one end portion to which the second airtight packing element (221) is coupled and fixed and the other end portion to which a plunger element (122) is coupled and fixed such that the second airtight packing element (221) is moved forward and backward simultaneously along with forward and backward movement of the plunger element (122).
[0109] The source material collecting means coupling module (212) is configured to include: a target material passage projecting element (212a) that extends and projects from the target material containing space module (211) by a certain length so as to be coupled to the source material collecting means (100) by enabling the end portion of the source material collecting means (100) to be inserted into and fixed in the target material passage projecting element; a target material flowing passage element (212b) that penetrates the target material passage projecting element (212a) and is connected to the target material containing space module (211) so as to enable the target material to be extracted from the separated source material in the target material containing space module (211); and a leakage preventive space element (212c) that is formed at an end portion of the target material flowing passage element (212b), into which the end portion of the source material collecting means (100) is inserted, and that has a diameter larger than an outer diameter of the target material flowing passage element (212b) so as to inhibit the source material or the target material from leaking outside due to surface tension when the source material collecting means (100) is decoupled.
[0110] Accordingly, the target material can be easily extracted from the separated source material contained in the target material containing barrel unit (210).
[0111] In other words, the source material is collected from the subject by using the source material collecting means (100) described above, and the target material extracting means coupling module (112) is inserted into and fixed to the source material collecting means coupling module (212) of the target material extracting means (200) in a state where the source material collecting space module (111) is filled with the collected source material, such that the source material, which is filled in the source material collecting space module (111), is separated by the centrifugal separator and is to be located in both the source material collecting space module (111) and the target material containing space module (211).
[0112] As described above, the source material collecting means (100) and the target material extracting means (200) which are coupled to each other by an operator is inserted into the centrifugal separator with the target material extracting means (200) being positioned at a bottom side, and then the source material is subjected to centrifugation.
[0113] In this manner, the source material is separated into layers formed in the source material collecting space module (111) and the target material containing space module (211) by the centrifugal separator, and the target material is to be extracted from the source material having the layers, as illustrated by an embodiment in
[0114] Hereinafter, a configuration of the target material extraction kit (1) for a centrifugal separator of the present invention will more specifically described. The plunger element (122) that is coupled to and decoupled from the first airtight packing-plunger connector element (123) and the second airtight packing-plunger connector element (222) according to a procedure of an operation (2) for extracting a target material can be formed by a male and female thread coupling method such that an operator can easily couple and decouple the plunger element to and from the first airtight packing-plunger connector element (123) and the second airtight packing-plunger connector element (222).
[0115] In addition, as illustrated in
[0116] In addition, the source material collecting barrel unit (110) is configured to further include: a first barrel handle (113) which is formed to project in a horizontal direction by a certain length from an outer circumferential edge of the other end portion of the source material collecting space module (111) such that an operator easily and stably handles the source material collecting barrel unit (110) and the source material collecting plunger unit (120); and a first airtight projection element (114) which is formed to project in a horizontal direction by a certain length from one side of an inner circumferential edge of the other end portion of the source material collecting space module (111) so as not only to maximize airtightness and hermeticity obtained by the source material collecting plunger unit (120) but also to inhibit the source material collecting plunger unit (120) from being freely decoupled from the source material collecting space module (111).
[0117] Accordingly, the operator can easily handle the source material collecting means (100).
[0118] The target material containing barrel unit (210) is also configured to further include, as the same function as that of the first airtight projection element, a second airtight projection element (214) which is formed to project in the horizontal direction by a certain length from one side of an inner circumferential edge of the other end portion of the target material containing space module (211) so as not only to maximize airtightness and hermeticity obtained by the target material discharging plunger unit (220) but also to inhibit the target material discharging plunger unit (220) from being freely decoupled from the target material containing space module (211).
[0119] Accordingly, the operator can easily handle the target material extracting means (200).
[0120] In this case, the free decoupling of the source material collecting plunger unit (120) from the source material collecting space module (111) and the target material discharging plunger unit (220) from the target material containing space module (211) means easy decoupling even by a relatively weak force and means the decoupling by an unintentional force other than a force which is applied by an operator to intentionally decouple the source material collecting plunger unit (120) and the target material discharging plunger unit (220) from the source material collecting space module (111) and the target material containing space module (211), respectively.
[0121] In addition, the source material collecting space module (111) has a first slope surface (111a) on one end portion thereof such that movement of the source material collecting plunger unit (120), movement of a source material, and movement of a target material are easily performed.
[0122] In other words, if the one end portion of the source material collecting space module (111) connected to the source material flowing passage element (112c) is formed in a flat surface, the source material collecting plunger unit (120) moves only when high pressure is momentarily generated. Hence, an operator cannot completely control the movement of the source material collecting plunger unit (120).
[0123] Consequently, the pressure inside the source material collecting space module (111) is distributed over the first slope surface (111a) such that the operator can completely control the movement of the source material collecting plunger unit (120).
[0124] Similarly, the target material containing space module (211) has a second slope surface (211a) on one end portion thereof such that movement of the target material discharging plunger unit (220), movement of a source material, and movement of a target material are easily performed.
[0125] In this case, the second slope surface (211a) is to be formed at an angle steeper than the first slope surface (111a) (a.sub.1≥a.sub.2) so as to completely extract a small amount of target material.
[0126] In other words, a small amount of target material located in the target material containing space module (211) gradually rises as an operator applies pressure to the target material discharging plunger unit (220) such that a thickness of a layer of target material to be extracted by the operator is gradually increased as the target material is moved toward the end portion of the target material containing space module (211) which has a cross-sectional area that is gradually decreased due to the second slope surface (211a) (effect of maximizing visibility of the target material).
[0127] Consequently, the second slope surface (211a) distributes the pressure inside the target material containing space module (211), similarly to the function of the first slope surface (111a), such that not only the operator can completely control the movement of the target material discharging plunger unit (220), but also the visibility of the target material to be extracted is maximized.
[0128] The above-described process is to be more specifically described with a method (2) for extracting a target material by using the target material extraction kit for a centrifugal separator.
[0129] As simply illustrated in
[0130] The target material extracting step (S200) is configured to include: an extracting means preparing step (S210) of preparing the target material extracting means (200); an extracting means coupling step (S220) of coupling the target material extracting means (200) to the source material collecting means (100) filled with the source material collected from the subject; a centrifugation preparing step (S230) of moving the source material, with which the source material collecting means (100) is filled, into the target material extracting means (200) coupled to the source material collecting means (100) by applying pressure to the source material collecting plunger unit (120); a plunger element decoupling step (S240) of decoupling the plunger element (122) from the source material collecting barrel unit (110) of the source material collecting means (100) before the source material collecting means is inserted into the centrifugal separator, a centrifuging step (S250) of separating a source material through centrifugation by inserting the source material collecting means (100), from which the source material collecting plunger unit (120) is decoupled, and the target material extracting means (200) into the centrifugal separator, a plunger element coupling step (S260) of coupling and fixing the plunger element (122) to the target material discharging plunger unit (220) of the target material extracting means (200) filled with the source material separated into layers by the centrifugal separator, a primary target material extracting step (S270) of injecting a part of the source material separated into layers, with which the target material containing barrel unit (210) is filled, into the source material collecting barrel unit (110) by applying pressure to the plunger element (122) coupled and fixed to the target material discharging plunger unit (220) to move the target material discharging plunger unit forward; a syringe body coupling step (S280) of decoupling the source material collecting means (100) from the target material extracting means (200) after the primary target material extracting step (S270) and coupling and fixing a syringe body (SY), which is to be filled with a target material, to the target material extracting means (200); a secondary target material extracting step (S290) of injecting a part of the source material separated into layers, which remains in the target material containing barrel unit (210), into the syringe body (SY) by re-applying pressure to the plunger element (122) coupled and fixed to the target material discharging plunger unit (220) to move the target material discharging plunger unit forward; and a target material preparation finishing step (S300) of decoupling the syringe body (SY) from the target material extracting means (200) and finishing a target material extracting operation.
[0131] Accordingly, the target material can be easily extracted from the source material.
[0132] For example, when the source material is blood, and the target material which is to be extracted from the blood is platelet rich plasma (PRP) and platelet poor plasma (PPP), the extraction is as follows, with reference to
[0133] (a) illustrates a state where a subject's blood is collected in the source material collecting means (100).
[0134] (b) illustrates a state where the source material collecting means (100), in which the subject's blood is collected, is coupled to the target material extracting means (200).
[0135] (c) illustrates a state where centrifugation is prepared by moving a certain amount of blood, with which the source material collecting means (100) is filled, into the target material extracting means (200) (a state where a certain amount of blood is moved into the target material extracting means (200) before the centrifugation).
[0136] (d) illustrates a state after the plunger element (122) is decoupled from the source material collecting barrel unit (110) from the state illustrated in (c) described above, the source material collecting means is inserted into the centrifugal separator, and the blood is subjected to the centrifugation.
[0137] (e) illustrates a state where the plunger element (122) decoupled from the source material collecting barrel unit (110) from (c) described above is coupled to the second airtight packing-plunger connector element (222), and a start of an extraction operation for extracting the target material is prepared.
[0138] (f) illustrates a state where the plunger element (122) in (e) is pressed in an arrow direction, and the platelet poor plasma (PPP) which is a first target material is extracted by an extraction operation performed by an operator.
[0139] (g) illustrates a state where the source material collecting means (100) filled with the platelet poor plasma (PPP) which is the first target material extracted from (f) is decoupled from the target material extracting means (200).
[0140] (h) illustrates a state of preparing a syringe body (SY) into which the platelet rich plasma (PRP) that is a secondary target material is to be extracted.
[0141] (i) illustrates a state where the syringe body (SY) is coupled to the target material extracting means (200).
[0142] (j) illustrates a state where the plunger element (122) coupled to the second airtight packing-plunger connector element (222) is re-pressed in the arrow direction, similarly to (f) described above, and the platelet rich plasma (PRP) which is a secondary target material is extracted into the syringe body (SY) by the extraction operation performed by an operator.
[0143] (k) illustrates a state where the syringe body (SY) filled with platelet rich plasma (PRP) which is the secondary target material is decoupled from the target material extracting means (200).
[0144] Then, the first target material (PPP) and the secondary target material (PRP) are both extracted from the blood, and remaining red blood cells remains as it is in the target material extracting means (200) to be easily disposed.
[0145] In this manner, in the method (2) for extracting a target material described above, a needling operation for extracting a target material by using a needle and a dropping operation for dropping a certain small amount of source material to the floor and removing the certain small amount of source material are omitted such that an extraction operation environment is cleanly and hygienically maintained, and easiness of the extraction operation of the target material is maximized.
[0146] For reference, in the operation (2) for extracting a target material by using the target material extraction kit for a centrifugal separator of the present invention, preparation of the target material extracting means (200) in the extracting means preparing step (S210) means that the target material discharging plunger unit (220) is prepared to be coupled to the source material collecting means (100) in a state where the target material discharging plunger unit is positioned at a specific position in the target material containing barrel unit (210) (the specific position of the target material discharging plunger unit (220) means that the target material discharging plunger unit (220) is positioned at the uppermost end in an opposite direction of a direction in which the target material extracting means (200) coupled to the source material collecting means (100) is inserted into the centrifugal separator).
[0147] The above-described process is performed such that minimum air remains inside the target material extracting means (200) which is to be coupled to the source material collecting means (100).
[0148] In other words, when pressure which is to be applied by the centrifugal separator to the inside of the source material collecting means (100) and the target material extracting means (200) is minimized, and the locations of the first airtight packing element (121) and the second airtight packing element (221) can be completely controlled when locations of the first airtight packing element (121) and the second airtight packing element (221) are simultaneously changed by the plunger element (122).
[0149] In addition, when the subject's blood is collected into the source material collecting means (100), a certain amount of anticoagulant has to be included (to inhibit coagulation of blood).
[0150] In addition, the ‘source material’ in the present invention can be mainly defined as blood, as described with the example in the above description of
[0151] As described above, the present invention is a result of modification of Prior Technology 1 that is applied and registered by the present applicants, and lack of description for a part other than the technical features of the present invention which are described in this specification is replaced with technical contents of Prior Technology 1.
[0152] In addition, compared to Prior Technology 1, the target material extraction kit (1) for a centrifugal separator of the present invention is configured as follows. A volume of the source material which can be collected into the source material collecting barrel unit (110) is 20 ml. A volume of the target material containing barrel unit (210) is increased to 12.5 ml. The target material containing barrel unit (210) contains 100% of red blood cells (RBC), 100% of platelet rich plasma (PRP), and a part of platelet poor plasma (PPP), and thereby the target material can be easily extracted from the source material by using the method (2) for extracting a target material as described above.
[0153] On the other hand, a second embodiment of the present invention has the same configuration as that of the first embodiment; however, the source material collecting means coupling module (212) formed at the target material containing barrel unit (210) has a structure of a luer-lock tip such that a coupling force to the target material extracting means coupling module (112) formed at the source material collecting barrel unit (110) can be improved and the sealing force is maximized so as to inhibit the source material and the target material from leaking.
[0154] In this case, when the source material collecting means coupling module (212) is configured to have a luer-lock tip structure, the target material extracting means coupling module (112) is also formed to have a structure corresponding thereto.
[0155] In addition, as another embodiment of the present invention, a third embodiment is configured to have the same configuration as that of any one embodiment of the first embodiment and the second embodiment described above. However, as illustrated in
[0156] As described above, the present invention is not limited to the described embodiment, and it is obvious for those who have common knowledge in the art to variously modify and change the present invention without departing from the idea and the scope of the present invention.
[0157] Hence, since the present invention can be realized as various embodiments without departing from the technical idea or the major feature, the embodiments of the present invention are only provided as simple examples and are not to be construed narrowly but can be variously modified.
INDUSTRIAL APPLICABILITY
[0158] The present invention relates to a target material extraction kit for a centrifugal separator and a method for extracting a target material by using the same and can be applied to a manufacturing business of manufacturing extraction kits and a sales business thereof, hospitals and research institutes where the operation for extracting the target material using the extraction kit according to the present invention is performed, and a promotion of the medical industry and the research industry.