Microfluidic chip capable of finely adjusting coaxial alignment of capillary tubes

Abstract

A microfluidic chip includes an integrated chip support (1), a continuous phase liquid inlet (1-6), an intermediate phase liquid inlet (1-7), a dispersed phase liquid inlet (2), an injection capillary tube (3), a collection capillary tube (4), a collection port (5), capillary tube nesting assemblies (6) and capillary tube coaxial fine adjustment assemblies (7). The chip support (1) is provided with threaded holes (1-1), sealing holes (1-2), capillary tube coaxial alignment holes (1-3), adjusting holes (1-4) and positioning holes (1-5). The injection capillary tube (3) and the collection capillary tube (4) present a three-dimensional coaxial alignment under the combined adjustment of the adjusting holes (1-4) and the capillary tube coaxial fine adjustment assemblies (7), and the capillary tube nesting assemblies (6) can realize the fixing and sealing of the capillary tubes in the chip support (1).

Claims

1. A microfluidic chip capable of finely adjusting coaxial alignment of capillary tubes, comprising a dispersed phase liquid inlet (2), an injection capillary tube (3), a collection capillary tube (4) and a collection port (5), further comprising an integrated chip support (1), capillary tube nesting assemblies (6) and capillary tube coaxial fine adjustment assemblies (7); wherein threaded holes (1-1), sealing holes (1-2), capillary tube coaxial alignment holes (1-3), adjusting holes (1-4) and positioning holes (1-5) are symmetrically formed on two sides of the integrated chip support (1), wherein the threaded holes (1-1), the sealing holes (1-2) and the capillary tube coaxial alignment holes (1-3) are sequentially connected; a continuous phase liquid inlet (1-6) and an intermediate phase liquid inlet (1-7) are further formed on the integrated chip support (1); there are two capillary tube nesting assemblies (6) arranged on two sides of the microfluidic chip respectively and used for fixing the injection capillary tube (3) and the collection capillary tube (4), and each of the capillary tube nesting assemblies comprises a fastener (6-1), a spacer sleeve 1 (6-2), an O-shaped sealing ring (6-3), a spacer sleeve 2 (6-4) and an O-shaped adjusting ring (6-5); there are 6 capillary tube coaxial fine adjustment assemblies (7), each of the capillary tube coaxial fine adjustment assemblies comprises a set screw (7-1) and a sealing gasket (7-2), and the set screws (7-1) are inserted into the adjusting holes (1-4) after being sleeved with the sealing gaskets (7-2); there are 6 adjusting holes (1-4), 3 adjusting holes are formed on each of the two sides of the integrated chip support (1) and arranged at 120 degrees, and the adjusting holes communicate with the sealing holes (1-2), and are right opposite to the O-shaped adjusting rings (6-5); and the injection capillary tube (3) and the collection capillary tube (4) present a three-dimensional coaxial alignment under the combined adjustment of the adjusting holes (1-4) and the capillary tube coaxial fine adjustment assemblies (7).

2. The microfluidic chip according to claim 1, wherein cross sections of the injection capillary tube (3) and the collection capillary tube (4) are round, opposite ends of the injection capillary tube and the collection capillary tube are tapered ends, and the other ends are flat port ends.

3. The microfluidic chip according to claim 1, wherein the continuous phase liquid inlet (1-6) and the intermediate phase liquid inlet (1-7) communicate with the capillary tube coaxial alignment holes (1-3) in a right opposite manner on an injection capillary tube side and a collection capillary tube side respectively; the dispersed phase liquid inlet (2) is located at a flat port end of the injection capillary tube (3); and the collection port (5) is located at a flat port end of the collection capillary tube (4).

4. The microfluidic chip according to claim 1, wherein an internal diameter of a tapered end of the injection capillary tube (3) ranges from 50 ?m to 80 ?m, and an internal diameter of the tapered end of the collection capillary tube (4) ranges from 100 ?m to 160 ?m.

5. The microfluidic chip according to claim 1, wherein an interval between opposite tapered ends of the injection capillary tube (3) and the collection capillary tube (4) ranges from 50 ?m to 100 ?m.

6. The microfluidic chip according to claim 1, wherein sizes of the threaded holes (1-1) are M8*1.0, diameters of the sealing holes (1-2) are 4.0 mm, and diameters of the capillary tube coaxial alignment holes (1-3) are 1.5 mm.

7. The microfluidic chip according to claim 1, wherein sizes of the adjusting holes (1-4) are M6.

8. The microfluidic chip according to claim 1, wherein external threads of the fasteners (6-1) are matched with internal threads of the threaded holes (1-1), sizes of the spacer sleeves 1 (6-2) and the spacer sleeves 2 (6-4) are 4.0 mm*2.0 mm (external diameter*internal diameter), and sizes of the O-shaped sealing rings (6-3) and the O-shaped adjusting rings (6-5) are 4.0 mm*1.5 mm (external diameter*internal diameter).

9. The microfluidic chip according to claim 1, wherein the integrated chip support (1) is made of glass, and selected from a cylinder or a hexagonal prism, preferably, the hexagonal prism.

10. The microfluidic chip according to claim 1, wherein the continuous phase liquid inlet (1-6), the intermediate phase liquid inlet (1-7) and the dispersed phase liquid inlet (2) in the chip can be used in combination with a peristaltic pump, an injection pump or a pressure controller, so as to control a flow rate of each phase of liquid; and the collection port (5) can be connected to a photo-curing apparatus, a heater or a cryogenic freezer, so as to enable the micro-droplets obtained to undergo further processing.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) In order to be illustrative, instead of being limitative, the present disclosure will be described now according to preferred embodiments of the present disclosure, especially reference accompanying drawings, where,

(2) FIG. 1 is a schematic diagram of integral assembly of a microfluidic chip capable of finely adjusting coaxial alignment of capillary tubes according to the present disclosure;

(3) FIG. 2 is a schematic structural diagram of an integrated chip support;

(4) FIG. 3 is a schematic diagram of assembly of capillary tubes and capillary tube nesting assemblies; and

(5) FIG. 4 is a side view after capillary tube coaxial fine adjustment assemblies are arranged into an integrated chip support.

(6) Reference signs in the drawings are shown as follows: 1: integrated chip support; 1-1: threaded hole; 1-2: sealing hole; 1-3: capillary tube coaxial alignment hole; 1-4: adjusting hole; 1-5: positioning hole; 1-6: continuous phase liquid inlet; 1-7: intermediate phase liquid inlet; 2: dispersed phase liquid inlet; 3: injection capillary tube; 4: collection capillary tube; 5: collection port; 6: capillary tube nesting assembly; 6-1: fastener; 6-2: spacer sleeve 1; 6-3: O-shaped sealing ring; 6-4: spacer sleeve 2; 6-5: O-shaped adjusting ring; 7: capillary tube coaxial fine adjustment assembly; 7-1: set screw; 7-2: sealing gasket.

DETAILED DESCRIPTION OF THE INVENTION

(7) The present disclosure is further described below with reference to specific embodiments and accompanying drawings.

Embodiment 1: Preparation of Water-In-Oil-In-Water (W/O/W) Type Micro-Droplets by Using a Microfluidic Chip Capable of Finely Adjusting Coaxial Alignment of Capillary Tubes

(8) Specific Implementation Steps:

(9) 1. Assembly of the Chip

(10) A round capillary tube is machined into two sections of capillary tubes with lengths being 5 cm and with tapered ends by a capillary tube pulling instrument, the tapered ends of the capillary tubes are ground till inner diameters are 55 ?m and 110 ?m, and the capillary tubes are used as an injection capillary tube (3) and a collection capillary tube (4) respectively. The obtained glass capillary tubes are cleaned and dried to remove residual glass particles, and the collection capillary tube (4) is hydrophobically treated with octadecyltrimethoxysilane, cleaned with ethanol, and aired for later use.

(11) The hydrophobically treated injection capillary tube (3) is sequentially sleeved with a fastener (6-1), a spacer sleeve 1 (6-2), an O-shaped sealing ring (6-3), a spacer sleeve 2 (6-4) and an O-shaped adjusting ring (6-5) from a tapered end side, and then penetrates through a threaded hole (1-1) and a sealing hole (1-2) from one side of a hexagonal-prism-shaped chip support (1) to reach a middle of a capillary tube coaxial alignment hole (1-3) with a diameter being 1.5 mm, and fixing of the injection capillary tube (3) and sealing of a flat port side of a micro-channel structure are realized by screwing the fastener (6-1) and pressing the O-shaped sealing ring (6-3); and the collection capillary tube (4) is sequentially sleeved with the other group of the fastener (6-1), the spacer sleeve 1 (6-2), the O-shaped sealing ring (6-3), the spacer sleeve 2 (6-4) and the O-shaped adjusting ring (6-5) from a tapered end side, and then penetrates through the threaded hole (1-1) and the sealing hole (1-2) in this side from the other side of the chip support (1) to reach a middle of the capillary tube coaxial alignment hole (1-3), an interval between tapered ends of the injection capillary tube (3) and the collection capillary tube (4) is adjusted to be 70 ?m via an optical microscope by screwing the fastener (6-1), and the collection capillary tube (4) is screwed down and fixed by pressing the O-shaped sealing ring (6-3); where materials of the O-shaped sealing rings (6-3) and the O-shaped adjusting rings (6-5) are selected from silicone rubber.

(12) 3 set screws (7-1) are inserted into 3 corresponding adjusting holes (1-4) in one side of the hexagonal-prism-shaped chip support (1) respectively after being sleeved with sealing gaskets (7-2), and are right opposite to the O-shaped adjusting ring (6-5) sleeved on the injection capillary tube (3); similarly, 3 set screws (7-1) sleeved with the sealing gaskets (7-2) are inserted into 3 adjusting holes (1-4) in the other side respectively, and are right opposite to the O-shaped adjusting ring (6-5) sleeved on the collection capillary tube (4); and relative angles of the injection capillary tube (3) and the collection capillary tube (4) in the micro-channel are adjusted respectively via observation by the optical microscope by screwing the 3 set screws (7-1) arranged at 120 degrees and pressing the O-shaped adjusting ring (6-5), and the hexagonal-prism-shaped chip support (1) is sequentially rotated to adjust relative positions of the capillary tubes at different angles, finally ensuring 360 degrees coaxial alignment of the injection capillary tube (3) and the collection capillary tube (4).

(13) 2. Preparation of Micro-Droplets

(14) In the embodiment, a 2 wt % PVA aqueous solution is selected as a dispersed phase, a 1 wt % Span 80 liquid paraffin solution is selected as an intermediate phase, a 5 wt % PVA aqueous solution is selected as a continuous phase, the dispersed phase, intermediate phase and continuous phase solutions are filled into 3 screw injectors respectively, and the screw injectors are installed on an injection pump.

(15) Two ends of a PTFE tubing with an external diameter being 1/16 are sleeved with a PEEK connector with an M6 thread and a pressing ring, bottoms of the pressing rings are flush with end faces of the tubing, one end is connected with the screw injector filled with the dispersed phase solution by a Luer taper, and the other end is connected with the fastener (6-1) on one side of the injection capillary tube (3); and similarly, the screw injectors filled with the intermediate phase solution and the continuous phase solution are connected with an intermediate phase liquid inlet (1-7) and a continuous phase liquid inlet (1-6) of the chip by PEEK connectors and PTFE tubings respectively.

(16) One end of a PTFE tubing with an external diameter of 1/16 is sleeved with a PEEK connector with an M6 thread and a pressing ring and connected with the fastener (6-1) on one side of the collection capillary tube (4), so that a collection port (5) is connected with the PTFE tubing, and the other end of the PTFE tubing can be put into a glass beaker.

(17) Flow rates of the dispersed phase, the intermediate phase and the continuous phase are set as 0.3-0.4 ml/h, 0.2-0.5 ml/h and 1.6-2.0 ml/h respectively, the injection pump is switched on, the flow rate of each phase is adjusted, and generation of the micro-droplets in the micro-channel is observed under the optical microscope. When stable W/O/W double-emulsion micro-droplets are formed in the collection capillary tube (4), the micro-droplets are collected by a glass beaker filled with a 5 wt % PVA aqueous solution.

(18) After the micro-droplets are prepared, the chip should be cleaned for next use, and ethanol and deionized water are used as cleaning fluids. Firstly, injection of three-phase fluids is stopped, and the injectors used by the three-phase fluids are replaced with injectors filled with the ethanol. The drive injection pump of the three-phase fluids is switched on till an oil-phase solution or an oil-water mixture in the chip is completely removed, the injectors are replaced with injectors filled with the deionized water, the chip is repeatedly cleaned and then continues to be cleaned with the ethanol, and the chip can be reused after being completely cleaned and aired.

(19) When the capillary tubes in the chip are blocked, the capillary tubes are detached by screwing capillary tube nesting assemblies (6) and loosening the set screws (7-1), and after the capillary tubes are dredged and cleaned, the chip can continue to be assembled and used.

Embodiment 2: Preparation of Oil-In-Water-In-Oil (O/W/O) Type Micro-Droplets by Using a Microfluidic Chip Capable of Finely Adjusting Coaxial Alignment of Capillary Tubes

(20) The embodiment is basically the same as Embodiment 1, and mainly differs from it in materials, sizes and structures of some assemblies during chip assembly, as well as compositions of three-phase fluids.

(21) Specific Implementation Steps:

(22) 1. Assembly of the Chip

(23) A round capillary tube is machined into two sections of capillary tubes with lengths being 5 cm and with tapered ends by a capillary tube pulling instrument, the tapered ends of the capillary tubes are ground till inner diameters are 60 ?m and 120 ?m, and the capillary tubes are used as an injection capillary tube (3) and a collection capillary tube (4) respectively. The obtained glass capillary tubes are cleaned and dried to remove residual glass particles, the injection capillary tube (3) is hydrophilically treated with a Piranha solution (H.sub.2SO.sub.4: H.sub.2O.sub.2=7/3, V/V), the collection capillary tube (4) is hydrophobically treated with octadecyltrimethoxysilane, and then the capillary tubes are respectively cleaned with ethanol and aired for later use.

(24) The hydrophilically treated injection capillary tube (3) is sequentially sleeved with a fastener (6-1), a spacer sleeve 1 (6-2), an O-shaped sealing ring (6-3), a spacer sleeve 2 (6-4) and an O-shaped adjusting ring (6-5) from a tapered end side, and then penetrates through a threaded hole (1-1) and a sealing hole (1-2) from one side of a micro-channel structure of a hexagonal-prism-shaped chip support (1) to reach a middle of a capillary tube coaxial alignment hole (1-3) with a diameter being 1.5 mm, and fixing of the injection capillary tube (3) and sealing of this side of the micro-channel structure are realized by screwing the fastener (6-1) and pressing the O-shaped sealing ring (6-3); and the hydrophobically treated collection capillary tube (4) is sequentially sleeved with the other group of the fastener (6-1), the spacer sleeve 1 (6-2), the O-shaped sealing ring (6-3), the spacer sleeve 2 (6-4) and the O-shaped adjusting ring (6-5) from a tapered end side, and then penetrates through a threaded hole (1-1) and a sealing hole (1-2) in this side from the other side of the micro-channel structure to reach a middle of the capillary tube coaxial alignment hole (1-3), an interval between tapered ends of the injection capillary tube (3) and the collection capillary tube (4) is adjusted to be 100 ?m via an optical microscope by screwing the fastener (6-1), and the collection capillary tube (4) is screwed down and fixed by pressing the O-shaped sealing ring (6-3); where materials of the O-shaped sealing rings (6-3) and the O-shaped adjusting rings (6-5) are selected from fluorine rubber.

(25) 3 set screws (7-1) are inserted into 3 corresponding adjusting holes (1-4) in one side of the hexagonal-prism-shaped chip support (1) respectively after being sleeved with sealing gaskets (7-2), and are right opposite to the O-shaped adjusting ring (6-5) sleeved on the injection capillary tube (3); similarly, 3 set screws (7-1) sleeved with the sealing gaskets (7-2) are inserted into 3 adjusting holes (1-4) in the other side respectively, and are right opposite to the O-shaped adjusting ring (6-5) sleeved on the collection capillary tube (4); and relative angles of the injection capillary tube (3) and the collection capillary tube (4) in the micro-channel are adjusted respectively via observation by the optical microscope by screwing the 3 set screws (7-1) arranged at 120 degrees and pressing the O-shaped adjusting ring (6-5), and the hexagonal-prism-shaped chip support (1) is sequentially rotated to adjust relative positions of the capillary tubes at different angles, finally ensuring 360 degrees coaxial alignment of the injection capillary tube (3) and the collection capillary tube (4).

(26) 2. Preparation of Micro-Droplets

(27) In the embodiment, a 8 wt % PLGA dichloromethane solution is selected as a dispersed phase, an aqueous solution containing 1 wt % PVA aqueous solution and 0.5 wt % sodium alginate is selected as an intermediate phase, a 10 wt % Span 80 methylbenzene solution is selected as a continuous phase, the dispersed phase, intermediate phase and continuous phase solutions are filled into 3 screw injectors respectively, and the screw injectors are installed on an injection pump.

(28) Two ends of a PEEK tubing with an external diameter being 1/16 are each sleeved with a PEEK connector with an M6 thread and a pressing ring, bottoms of the pressing rings are flush with end faces of the tubing, one end is connected with the screw injector filled with the dispersed phase solution by a Luer taper, and the other end is connected with the fastener (6-1) on one side of the injection capillary tube (3); and similarly, the screw injectors filled with the intermediate phase solution and the continuous phase solution are connected with an intermediate phase liquid inlet (1-7) and a continuous phase liquid inlet (1-6) of the chip by PEEK connectors and PTFE tubings respectively.

(29) One end of a PEEK tubing with an external diameter of 1/16 is sleeved with a PEEK connector with an M6 thread and a pressing ring and connected with the fastener (6-1) on one side of the collection capillary tube (4), so that a collection port (5) is connected with the PTFE tubing, and the other end of the PTFE tubing can be put into a glass beaker.

(30) Flow rates of the dispersed phase, the intermediate phase and the continuous phase are set as 0.4-0.8 ml/h, 0.4-0.85 ml/h and 2.0-6.0 ml/h respectively, the injection pump is switched on, the flow rate of each phase is adjusted, and generation of the micro-droplets in the micro-channel is observed under the optical microscope. When stable O/W/O double-emulsion micro-droplets are formed in the collection capillary tube (4), the micro-droplets are collected by a glass beaker filled with a 20 mM calcium chloride aqueous solution.

(31) After the micro-droplets are prepared, the chip should be cleaned for next use. Firstly, injection of three-phase fluids is stopped, and the injectors used by the three-phase fluids are replaced with injectors filled with dichloromethane. The drive injection pump of the three-phase fluids is switched on till a residual solution in the chip is completely removed, the injectors are replaced with injectors filled with ethanol, the chip is repeatedly cleaned for 2 times, and the chip can be reused after being completely cleaned and aired.

(32) When the capillary tubes in the chip are blocked, the capillary tubes are detached by screwing capillary tube nesting assemblies (6) and loosening the set screws (7-1), and after the capillary tubes are dredged and cleaned, the chip can continue to be assembled and used.

Embodiment 3: Preparation of Water-In-Oil-In-Water (W/O/W) Type Micro-Droplets by Using a Microfluidic Chip Capable of Finely Adjusting Coaxial Alignment of Capillary Tubes

(33) The embodiment is basically the same as Embodiment 1 and Embodiment 2, and mainly differs from them in materials, shapes, sizes and structures of some assemblies during chip assembly, as well as compositions of three-phase fluids.

(34) Specific Implementation Steps:

(35) 1. Assembly of the Chip

(36) A round capillary tube is machined into two sections of capillary tubes with lengths being 5 cm and with tapered ends in one ends by a capillary tube pulling instrument, the tapered ends of the capillary tubes are ground till inner diameters are 75 ?m and 150 ?m, and the capillary tubes are used as an injection capillary tube (3) and a collection capillary tube (4) respectively. The obtained glass capillary tubes are cleaned and dried to remove residual glass particles, and the injection capillary tube (3) is hydrophobically treated with octadecyltrimethoxysilane, then cleaned with ethanol, and aired for later use.

(37) The hydrophobically treated injection capillary tube (3) is sequentially sleeved with a fastener (6-1), a spacer sleeve 1 (6-2), an O-shaped sealing ring (6-3), a spacer sleeve 2 (6-4) and an O-shaped adjusting ring (6-5) from a tapered end side, and then penetrates through a threaded hole (1-1) and a sealing hole (1-2) from one side of a micro-channel structure of a cylindrical chip support (1) to reach a middle of a capillary tube coaxial alignment hole (1-3) with a diameter being 1.5 mm, and fixing of the injection capillary tube (3) and sealing of this side of the micro-channel structure are realized by screwing the fastener (6-1) and pressing the O-shaped sealing ring (6-3); and the collection capillary tube (4) is sequentially sleeved with the other group of the fastener (6-1), the spacer sleeve 1 (6-2), the O-shaped sealing ring (6-3), the spacer sleeve 2 (6-4) and the O-shaped adjusting ring (6-5) from a tapered end side, and then penetrates through the threaded hole (1-1) and the sealing hole (1-2) in this side from the other side of the micro-channel structure to reach a middle of the capillary tube coaxial alignment hole (1-3), an interval between tapered ends of the injection capillary tube (3) and the collection capillary tube (4) is adjusted to be 100 ?m via an optical microscope by screwing the fastener (6-1), and the collection capillary tube (4) is screwed down and fixed by pressing the O-shaped sealing ring (6-3); where materials of the O-shaped sealing rings (6-3) and the O-shaped adjusting rings (6-5) are selected from nitrile rubber. 3 set screws (7-1) are inserted into 3 corresponding adjusting holes (1-4) in one side of the cylindrical chip support (1) respectively after being sleeved with sealing gaskets (7-2), and are right opposite to the O-shaped adjusting ring (6-5) sleeved on the injection capillary tube (3); and similarly, the 3 set screws (7-1) sleeved with the sealing gaskets (7-2) are inserted into 3 adjusting holes (1-4) in the other side respectively, and are right opposite to the O-shaped adjusting ring (6-5) sleeved on the collection capillary tube (4); and relative angles of the injection capillary tube (3) and the collection capillary tube (4) in the micro-channel are adjusted respectively via observation by the optical microscope by screwing the 3 set screws (7-1) arranged at 120 degrees and pressing the O-shaped adjusting ring (6-5), and the cylindrical chip support (1) is sequentially rotated to adjust relative positions of the capillary tubes at different angles, finally ensuring 360 degrees coaxial alignment of the injection capillary tube (3) and the collection capillary tube (4).

(38) 2. Preparation of Micro-Droplets

(39) In the embodiment, a 1 wt % PVA aqueous solution is selected as a dispersed phase, a 4-cyano-4-pentylbiphenyl (a liquid crystal system being a mobile phase at room temperature, not dissolved in water) is selected as an intermediate phase, an aqueous solution containing 1 wt % PVA aqueous solution and 60 wt % glycerol is selected as a continuous phase, the dispersed phase, intermediate phase and continuous phase solutions are filled into 3 screw injectors respectively, and the screw injectors are installed on an injection pump.

(40) Two ends of a PEEK tubing with an external diameter being 1/16 are each sleeved with a PEEK connector with an M6 thread and a pressing ring, bottoms of the pressing rings are flush with end faces of the tubing, one end is connected with the screw injector filled with the dispersed phase solution by a Luer taper, and the other end is connected with the fastener (6-1) on one side of the injection capillary tube (3); and similarly, the screw injectors filled with the intermediate phase solution and the continuous phase solution are connected with an intermediate phase liquid inlet (1-7) and a continuous phase liquid inlet (1-6) of the chip by PEEK connectors and PTFE tubings respectively.

(41) One end of a PEEK tubing with an external diameter of 1/16 is sleeved with a PEEK connector with an M6 thread and a pressing ring and connected with the fastener (6-1) on one side of the collection capillary tube (4), so that a collection port (5) is connected with the PTFE tubing, and the other end of the PTFE tubing can be put into a glass beaker.

(42) Flow rates of the dispersed phase, the intermediate phase and the continuous phase are set as 0.1-0.5 ml/h, 0.25-0.5 ml/h and 1.0-5.0 ml/h respectively, the injection pump is switched on, the flow rate of each phase is adjusted, and generation of the micro-droplets in the micro-channel is observed under the optical microscope. When stable W/O/W double-emulsion micro-droplets are formed in the collection capillary tube (4), the micro-droplets are collected by a glass beaker filled with a 1 wt % PVA aqueous solution and 60 wt % glycerol aqueous solution.

(43) After the micro-droplets are prepared, the chip should be cleaned for next use. Firstly, injection of three-phase fluids is stopped, and the injectors used by the three-phase fluids are replaced with injectors filled with the ethanol. The drive injection pump of the three-phase fluids is switched on till the liquid crystal system or other mixtures in the chip are completely removed, the injectors are replaced with injectors filled with deionized water, the chip is repeatedly cleaned and then continues to be cleaned with ethanol, and the chip can be reused after being completely cleaned and aired.

(44) When the capillary tubes in the chip are blocked, the capillary tubes are detached by screwing capillary tube nesting assemblies (6) and loosening the set screws (7-1), and after the capillary tubes are dredged and cleaned, the chip can continue to be assembled and used.

(45) The above specific implementations do not constitute a limitation to the scope of protection of the present disclosure. Those skilled in the art should understand that various modifications, combinations, sub-combinations and replacements can occur depending on design requirements and other factors. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present disclosure should fall within the scope of protection of the present disclosure.