Light measurement apparatus for reaction vessel and light measurement method

Abstract

The invention relates to a method and apparatus for measuring the optical state on the inside of a reaction vessel. The apparatus includes: linking portions that optically connect with the interior of the reaction vessel that is linkable with reaction vessels, a connecting end arranging body having an arranging surface that arranges and supports along a predetermined path, connecting ends to which is provided back ends of the light guide portions, the front ends thereof being provided to the linking portions, the connecting ends being provided to the linking portions; a measurement device having measuring ends that are successively optically connectable with the connecting ends, and in which light based on the optical state is receivable by optical connections between the connecting ends and the measuring ends; and a light guide switching mechanism that successively optically connects the connecting ends and the measuring ends.

Claims

1. A light measurement apparatus for a reaction vessel comprising: two or more linking portions to which is provided front ends of one or two or more light guide portions that are directly or indirectly linkable with two or more reaction vessels and optically connect with the interior of the reaction vessel to which it is linked; a connecting end arranging body that has an arranging surface that arranges and supports along a predetermined path, two or more connecting ends, to which is provided back ends of the light guide portions, in which the front ends thereof are provided to the linking portions, the connecting ends are provided corresponding to the respective linking portions; a measurement device that has one or two or more measuring ends that are successively optically connectable with the respective two or more connecting ends along the predetermined path on the arranging surface, and in which light based on an optical state within the reaction vessels is receivable by means of optical connections between the two or more connecting ends and the measuring ends, the measuring ends are provided approaching or making contact with the arranging surface; and a light guide switching mechanism that relatively moves the respective two or more connecting ends arranged on the connecting end arranging body and the respective measuring ends along the predetermined path on the arranging surface such that they are successively optically connected, in a state that the two or more linking portions are held to be linked to the respective two or more reaction vessels, wherein the measurement device comprises a plurality of types of measurement devices each including a photoelectric element, and provided with respect to the respective measurement devices, and at the very least between adjacent measurement devices, are a modulator that modulates the intensity of the light to be received by the photoelectric elements provided in the interior of the respective measurement devices at mutually different predetermined frequencies, and a tuner that, with regard to the light received by the respective measurement devices, performs tuning to the predetermined frequencies, and obtains the corresponding intensity of the received light, such that optical crosstalk mainly due to the entry of excitation light from adjacent measurement devices is prevented.

2. A light measurement apparatus for a reaction vessel according to claim 1, wherein at least a portion of the light guide portion has flexibility.

3. A light measurement apparatus for a reaction vessel according to claim 1, wherein the measurement device comprises a plurality of types of specific wavelength measurement devices that are able to receive the respective light of specific wavelengths or specific wavelength bands, the specific wavelength measurement devices having at least one measuring end that is successively optically connectable with the connecting ends along the predetermined path, and the respective predetermined frequencies of the modulators corresponding to the specific wavelength measurement devices being mutually different, at least between adjacent specific wavelength measurement devices, and in a case where the light based on the optical state is a fluorescent light, the specific wavelength measurement devices are provided with an irradiation source that irradiates a predetermined excitation light that excites the corresponding fluorescent light of a specific wavelength or a specific wavelength band, and a light receiving portion that is able to receive the fluorescent light of the specific wavelength or the specific wavelength band, and the modulators that correspond to the respective specific wavelength measurement devices are provided with an excitation light modulation portion that modulates the predetermined excitation lights at the predetermined frequency, and the tuners are provided with a bandpass filter circuit or a lock-in amplifier that, with respect to the light received by the light receiving portion, extracts the intensity data of the light having the predetermined frequencies.

4. A light measurement apparatus according to claim 1, that has two or more linking portions provided on a light guide stage, and a stage transfer mechanism that relatively moves the light guide stage with respect to the reaction vessels such that the linking portion is simultaneously directly or indirectly linked with the two or more reaction vessels.

5. A light measurement apparatus for a reaction vessel according to claim 1, provided with a temperature controller having a temperature source that is provided making contact with or approaching the lower side wall sections of the reaction vessels, and a heating portion provided such that it is able to make contact with or approach the upper side wall sections of the reaction vessels positioned further on an upper side than the lower side wall sections of the reaction vessels, and that has a heat source that is able to heat the upper side wall sections.

6. A light measurement apparatus for a reaction vessel according to claim 1, wherein a linking portion, which is directly or indirectly linkable with the reaction vessels and is provided with an optical element in the interior, is made from a material that is usable to a temperature higher than the dew point of water vapor, and has a thermal conductivity smaller than 1.0 W/(m.Math.K).

7. A light measurement apparatus for a reaction vessel according to claim 1, wherein front ends of a light guide portion bundle which comprises a plurality of light guide portions, are provided to the respective linking portions, back ends of a light guide portion bundle of a portion of the light guide portion bundle are provided to first connecting ends of the connecting end arranging body, a portion or all of the remainder of the light guide portion bundle is provided to second connecting ends of the connecting end arranging body, the predetermined path comprises a first path and a second path, and the connecting end arranging body has a plurality of pairs of first connecting ends and second connecting ends that are optically connected with the linking portion, and a single pair of a third connecting end and a fourth connecting end that is optically mutually connected by means of the light guide portion, and the first measuring end provided to the measurement device is provided relatively movable along a first path, which comprises a plurality of the first connecting ends and a single third connecting end, and the second measuring end provided to the measurement device is provided relatively movable along a second path, which comprises a plurality of the second connecting ends and a single fourth connecting end, and the first measuring end is optically connect with the irradiation source of the measurement device, and the second measuring end is connected with the light receiving portion of the measurement device, and for each pair, the first measuring end is successively connectable with the plurality of first connecting ends and the single third connecting end, and the second measuring end is successively connectable with the plurality of second connecting ends and the single fourth connecting end.

8. A light measurement apparatus for a reaction vessel comprising: a nozzle head provided with a suction-discharge mechanism that performs suction and discharge of gases, and one or two or more nozzles that detachably mount dispensing tips in which the suction and the discharge of liquids is possible by means of the suction-discharge mechanism; a vessel group having at the very least one or two or more liquid housing parts that house reaction solutions used for various reactions, a liquid housing part that houses a magnetic particle suspension in which magnetic particles that are able to capture a target compound are suspended, a liquid housing part that houses a sample, one or two or more liquid housing parts that house a solution for separating and extracting of the target compound, and two or more reaction vessels; a nozzle head transfer mechanism that makes an interval between the nozzle head and the vessel group relatively movable; a magnetic force part that is able to adsorb the magnetic particles on an inner wall of dispensing tips mounted on the nozzles; a light guide stage provided to the nozzle head and having two or more linking portions to which ends of one or two or more light guide portions, which have a flexibility, that are directly or indirectly linkable with the respective reaction vessels and optically connect with the interior of the linked reaction vessels, are provided; a connecting end arranging body having an arranging surface that arranges and supports along a predetermined path two or more connecting ends, to which back ends of the light guide portions, in which front ends thereof are provided to the linking portions, are provided, the connecting ends are provided corresponding to the respective linking portions; a measurement device having one or two or more measuring ends that are successively optically connectable with the respective two or more connecting ends along the predetermined path on the arranging surface, that is able to receive light based on an optical state within the reaction vessels by means of optical connections between the two or more connecting ends and the measuring ends, the measuring ends are provided approaching or making contact with the arranging surface; and a light guide switching mechanism provided along the predetermined path of the connecting end arranging body that relatively moves the respective two or more connecting ends and the respective measuring ends along the predetermined path on the arranging surface such that they become successively optically connected, in a state that the two or more linking portions are held to be linked to the respective two or more reaction vessels, wherein the measurement device comprises a plurality of types of measurement devices each including a photoelectric element, and the apparatus is configured to at the very least between adjacent measurement devices, modulate the intensity of the light to be received by the photoelectric elements provided in the interior of the respective measurement devices at mutually different predetermined frequencies, tune the light received by the measurement devices to the predetermined frequencies, and obtain the corresponding intensity of the received light, such that optical crosstalk mainly due to the entry of excitation light from adjacent measurement devices is prevented.

9. A light measurement method for a reaction vessel comprising: moving two or more linking portions provided with an end of one or two or more light guide portions, with respect to two or more reaction vessels; simultaneously directly or indirectly linking the reaction vessels and the linking portions and optically connecting the interior of the linked reaction vessels and the light guide portions; performing temperature control within the reaction vessels; guiding light from the reaction vessels to a connecting end arranging body having an arranging surface that arranges and supports along a predetermined path two or more connecting ends which are directly or indirectly optically linked to two or more reaction vessels, and to which back ends of the light guide portions, in which front ends thereof are provided to the linking portions, are provided, the connecting ends are provided corresponding to the respective linking portions; and successively optically connecting along the predetermined path the one or two or more measuring ends provided to a measurement device, which are provided approaching or in contact with the arranging surface, and the respective two or more connecting ends, in a state that the two or more linking portions are held to be linked to the respective two or more reaction vessels, by relatively moving the connecting end arranging body along the predetermined path on the arranging surface, to thereby make a plurality of types of measurement devices receive light based on an optical state within the reaction vessels, wherein the measurement device comprises a plurality of types of measurement devices each including a photoelectric element, and the method comprises at the very least between adjacent measurement devices, modulating the intensity of the light to be received by the photoelectric elements provided in the interior of the respective measurement devices at mutually different predetermined frequencies, tuning the light received by the measurement devices to the predetermined frequencies, and obtaining the corresponding intensity of the received light, such that optical crosstalk mainly due to the entry of excitation light from adjacent measurement devices is prevented.

10. A light measurement method for a reaction vessel according to claim 9, wherein an optical connection between the reaction vessel and the connecting ends is directly or indirectly linkable with two or more reaction vessels, and back ends of one or two or more light guide portions that optically connect with the interior of the linked reaction vessels, are provided on the connecting ends, and the optical connection is performed by relatively moving a light guide stage on which is provided the two or more linking portions provided with front ends of the light guide portions, with respect to the reaction vessel.

11. A light measurement method for a reaction vessel according to claim 9, wherein the front ends of a light guide portion bundle comprising a plurality of light guide portions are provided to the respective linking portions, the light guide portion bundle is such that the rear ends of a portion of the light guide portion bundle are provided to first connecting ends of the connecting end arranging body, a portion or all of the remaining rear ends of the light guide portion bundle are provided to second ends of the connecting end arranging body, the predetermined path comprises a first path and a second path, the connecting end arranging body has a plurality of pairs of first connecting ends and second connecting ends that are optically connected with the linking portion, and a single pair of a third connecting end and a fourth connecting end that is mutually optically connected by means of the light guide portion, and at the time the first measuring end provided to the measurement device relatively moves along the first path, which comprises a plurality of the first connecting ends and a single third connecting end, and the second measuring end provided to the measurement device relatively moves along the second path, which comprises the plurality of second connecting ends and a single fourth connecting end, the first measuring ends optically connect with the irradiation source of the measurement device, and the second measuring ends connect with the light receiving portion of the measurement device, and for each pair, the first measuring end successively connects with the plurality of first connecting ends and the single third connecting end, and the second measuring end successively connects with the plurality of second connecting ends and the single fourth connecting end.

12. A light measurement method for a reaction vessel according to claim 9, comprising: directly or indirectly linking an aperture of the reaction vessel and the linking portion; and at the time of performing temperature control within the reaction vessels, according to temperature control by a temperature controller, which has a temperature source provided making contact with or approaching lower side wall sections of the reaction vessels, heating upper side wall sections of the reaction vessels, which are positioned further on an upper side than the lower side wall sections, by means of a heat source of a heating portion, which is provided making contact with or approaching the upper side wall sections, and thereby preventing direct or indirect condensation of the linking portions.

13. A light measurement method for a reaction vessel comprising: detachably mounting dispensing tips on respective nozzles, which are provided to a nozzle head and perform the suction and the discharge of gases; separating a target compound by using a magnetic force part, a nozzle head transfer mechanism that relatively moves an interval between the nozzle head and a vessel group, a magnetic particle suspension housed within a vessel group, in which magnetic particles that are able to capture a target compound are suspended, a sample, and a solution for separating and extracting of a target compound; introducing the separated target compound and a reaction solution used for reactions to a plurality of reaction vessels provided to a vessel group; moving a light guide stage, which has two or more linking portions that are provided to the nozzle head and in which front ends of one or two or more light guide portions are also provided, with respect to the reaction vessels by means of, at the very least, the nozzle head transfer mechanism; directly or indirectly simultaneously linking the respective reaction vessels and the linking portions, and optically connecting the interior of the reaction vessels and the light guide portions that are linked; performing temperature control within the reaction vessels; guiding light from the reaction vessels to a connecting end arranging body that arranges and supports along a predetermined path two or more connecting ends, to which back ends of the light guide portions, in which front ends thereof are provided to the linking portions, are provided, the connecting ends are provided corresponding to the respective linking portions; and successively optically connecting one or two or more measuring ends that are provided to a measurement device, and provided approaching or making contact with an arranging surface, and the respective two or more connecting ends, in a state that the two or more linking portions are held to be linked to the respective two or more reaction vessels, along the predetermined path by moving the connecting end arranging body, to thereby make the measurement device receive the light based on an optical state within the reaction vessels, wherein the measurement device comprises a plurality of types of measurement devices each including a photoelectric element, and the method comprises, at the very least between adjacent measurement devices, modulating the intensity of the light to be received by the photoelectric elements provided in the interior of the respective measurement devices at mutually different predetermined frequencies, tuning the light received by the respective measurement devices to the predetermined frequencies, and obtaining the corresponding intensity of the received light, such that optical crosstalk mainly due to the entry of excitation light from adjacent measurement devices is prevented.

14. A light measurement method for a reaction vessel according to claim 13, wherein the measurement device comprises a plurality of types of measurement devices, and the method comprises at the time of guiding light from the reaction vessels to a connecting end arranging body, at the very least between adjacent measurement devices, modulating the intensity of the light to be received by the measurement devices at mutually different predetermined frequencies, and at the time the measurement device receives the light based on an optical state within the reaction vessels, tuning the light received by the measurement devices to the predetermined frequencies, and obtaining the corresponding intensity of the received light.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is an overall block-diagram showing a light measurement apparatus for a reaction vessel according to a first embodiment of the present invention.

(2) FIG. 2 is an overall perspective view showing the light measurement apparatus for a reaction vessel according to a first exemplary embodiment.

(3) FIG. 3 is a plan view showing enlarged, a vessel group of the light measurement apparatus for a reaction vessel shown in FIG. 2.

(4) FIG. 4 is a front view and a side view showing enlarged, a nozzle head of the light measurement apparatus for a reaction vessel shown in FIG. 2.

(5) FIG. 5 is a perspective view as viewed from the front side of the nozzle head shown in FIG. 4.

(6) FIG. 6 is a side view showing more specifically, the transfer mechanisms and the suction-discharge mechanism shown in FIG. 4.

(7) FIG. 7 is a perspective view showing more specifically, the suction-discharge mechanism 53 and the like shown in FIG. 4.

(8) FIG. 8 is a perspective view as viewed from the rear side of the nozzle head shown in FIG. 4.

(9) FIG. 9 is a cross-sectional view showing a state in which the linking portion shown in FIG. 4 is linked with a reaction vessel.

(10) FIG. 10 is a drawing showing the specific wavelength measurement device shown in FIG. 4.

(11) FIG. 11 is a perspective view showing a light guide stage, a connecting end arranging body, and a sealing lid transport mechanism of a light measurement apparatus for a reaction vessel according to a second exemplary embodiment.

(12) FIG. 12 is an enlarged perspective view showing the light guide stage shown in FIG. 11 with a portion cut away.

(13) FIG. 13 is an enlarged cross-sectional view of the linking portion shown in FIG. 12.

(14) FIG. 14 is a cross-sectional view showing an example of the sealing lid shown in FIG. 11.

(15) FIG. 15 is an enlarged perspective view of the sealing lid transporting body shown in FIG. 11.

(16) FIG. 16 is a cross-sectional view of the sealing lid transporting body shown in FIG. 15.

(17) FIG. 17 is a perspective view as viewed from the lower side of the sealing lid transporting body shown in FIG. 15.

(18) FIG. 18 is a schematic diagram showing an example of the positional relationship between the optical fiber ends provided on the linking portions and the reaction vessel.

(19) FIG. 19 is an overall block-diagram showing a light measurement apparatus for a reaction vessel according to a second embodiment of the present invention.

(20) FIG. 20 is a side view showing more specifically, the transfer mechanism and the suction-discharge mechanism according to a first exemplary embodiment of FIG. 19.

(21) FIG. 21 is a cross-sectional view showing a state in which a linking portion according to the first exemplary embodiment of FIG. 19 is linked with a reaction vessel.

(22) FIG. 22 is a cross-sectional view showing a state in which a linking portion according to a second exemplary embodiment of FIG. 19 is linked with a reaction vessel.

(23) FIG. 23 is a cross-sectional view showing a state in which a linking portion according to a third exemplary embodiment of FIG. 19 is linked with a reaction vessel.

(24) FIG. 24 is a drawing showing heating control according to a fourth exemplary embodiment of FIG. 19.

(25) FIGS. 25A, B and C are drawings showing the temperature-fluorescence intensity characteristics within a reaction vessel according to a fifth exemplary embodiment of FIG. 19.

(26) FIG. 26 is a drawing showing temperature measurement results of a ferrule within a linking portion according to a sixth exemplary embodiment of FIG. 19.

(27) FIG. 27 is a block diagram showing a measurement device of a light measurement apparatus for a reaction vessel according to a third embodiment of the present invention.

(28) FIG. 28 is a drawing showing connection between the connecting end arranging body and a measuring end according to a first exemplary embodiment of FIG. 27.

(29) FIGS. 29A, B, C, D, and E are drawings showing the effects between measurement devices with regard to the light to be received by the measurement devices of FIG. 27.

(30) FIG. 30 is a drawing showing the spacing of the linking portions and the spacing of the connecting ends of the connecting end arranging body of FIG. 27.

(31) FIG. 31 is a drawing showing a measurement possibility of a measurement device for the case of FIG. 30.

(32) FIG. 32 is a drawing showing connection between the connecting end arranging body and a measuring end according to a seventh embodiment of the present invention.

(33) FIG. 33 is a graph showing fluorescence intensities within the reaction vessels using the connecting end arranging body according to FIG. 32, and the standardized fluorescence intensities.

(34) FIG. 34 is a block diagram showing a measurement device of a light measurement apparatus for a reaction vessel according to an eighth embodiment of the present invention.

BEST MODE FOR CARRYING OUT THE INVENTION

(35) Next, an embodiment of the present invention is described with reference to the drawings. This embodiment is not to be interpreted as limiting the present invention unless particularly specified. Furthermore, in the embodiments or in the exemplary embodiments, the same objects are denoted by the same reference symbols, and the descriptions are omitted.

(36) FIG. 1 is shows a block-diagram of a light measurement apparatus for a reaction vessel 10 according to a first embodiment of the present invention.

(37) The light measurement apparatus for a reaction vessel 10 broadly has: a vessel group 20 in which a plurality (twelve in this example) of reaction vessel groups 23.sub.i (i=1, . . . , 12, omitted hereunder) are arranged; a nozzle head 50 that has a nozzle arranging portion 70 in which a plurality (twelve in this example) of nozzles 71.sub.i that detachably mount dispensing tips are arranged, and a light guide stage 32 that has a plurality (twelve in this example) of linking portions 31.sub.i provided with the ends of two or more light guide portions, which have a flexibility, that are directly or indirectly linkable with the apertures of the reaction vessels and optically connect to the linked reaction vessel interior; a measurement device 40 that is provided fixed to the nozzle head 50; a nozzle head transfer mechanism 51 that makes the nozzle head 50 movable in the X axis direction for example; a temperature controller 29 that performs predetermined temperature control with respect to the reaction vessel group 23.sub.i of the vessel group; a CPU+program 60 composed of a CPU, a ROM, a RAM, various types of external memory, communication functions such as a LAN, and a program stored in the ROM, and the like; and a control panel 13 having a display portion such as a liquid crystal display, and an operation portion, such as operation keys or a touch panel.

(38) The nozzle head 50 has: a stage Z axis transfer mechanism 35 that makes the light guide stage 32 movable in the Z axis direction with respect to the vessel group 20 independent of the nozzle arrangement portion 70; a nozzle Z axis transfer mechanism 75 that makes the nozzle arrangement portion 70 movable in the Z axis direction with respect to the vessel group 20 independent of the stage 30; a magnetic force part 57 that, by means of a magnet 571 provided on a narrow diameter portion 211ia of a dispensing tip 211i detachably mounted on the nozzle 71i such that it can approach and separate, is able to apply and remove a magnetic field with respect to the interior; a suction-discharge mechanism 53 that makes the suction and the discharge of liquids with respect to the dispensing tip 211i mounted on the nozzle 71i possible by performing the suction and the discharge of gases with respect to the nozzle 71i; and a punching mechanism 55 which is driven by the suction-discharge mechanism 53, for punching a film that covers the apertures of the liquid housing parts of the vessel group 20 to seal various liquids in advance. The stage transfer mechanism corresponds to the nozzle head transfer mechanism and the stage Z axis transfer mechanism.

(39) The nozzle head 50 further has a connecting end arranging body 30 that arranges and supports a plurality (twelve in this example) of connecting ends 34.sub.i, which are provided corresponding to the respective linking portions 31.sub.i and are provided with the back ends of optical fibers (bundle) 33.sub.i, which represent light guide portions in which the front ends thereof are provided to the linking portions 31.sub.i, such that, as an arranging surface, they are integrated along a predetermined path (a linear path along the Y axis direction in this example) provided on a vertical plane at a narrower spacing than the spacing between the linking portions 31.sub.i. Furthermore, the connecting end arranging body 30 is provided at a position that is separated from the light guide stage 32 and the reaction vessel group 23.sub.i.

(40) The measurement device 40 is able to respectively receive the light of specific wavelengths or specific wavelength bands of six types of fluorescent light, and also has six types of specific wavelength measurement devices 40.sub.j (j=1, . . . , 6, omitted hereunder) that are able to irradiate excitation light of six types of specific wavelengths or specific wavelength bands that are irradiated for the emission of light.

(41) The respective specific wavelength measurement devices 40.sub.j have measuring ends 44.sub.j that are provided approaching or making contact with the arrangement surface, and are successively connectable with the respective connecting ends 34.sub.i along the predetermined path (a linear path along the Y axis direction). Furthermore, the respective measuring ends 44.sub.j have two ends, namely a first measuring end 42.sub.j and a second measuring end 43.sub.j arranged along the Y axis direction. The first measuring ends 42.sub.j optically connect with an irradiation source provided to the specific wavelength measurement devices 40.sub.j. The second measuring ends 43.sub.j optically connect with a photoelectric device, such as a photomultiplier tube, provided to the specific wavelength measurement devices 40.sub.j.

(42) Furthermore, the nozzle head 50 has an arranging body Y axis transfer mechanism 41, which represents a light guide switching mechanism, that moves the connecting end arranging body 30 along the Y axis direction on the nozzle head 50 such that the respective connecting ends 34.sub.i arranged on the connecting end arranging body 30 and the respective measuring ends 44.sub.j are successively connected.

(43) Moreover, the light guide stage 32 has a heater 37, which represents the heating portion, for preventing condensation of the ends of the linking portions 31.sub.i or the mounted sealing lids 251.sub.i, which have transparency, by heating.

(44) The vessel group 20 comprises a plurality (twelve in this example) of exclusive regions 20.sub.i, in which one (in this example, one group corresponds to one) nozzle enters and the other nozzles do not enter, that correspond to the respective nozzles. The respective exclusive regions 20.sub.i have: a liquid housing part group 27.sub.i comprising a plurality of housing parts in which reagent solutions and the like are housed or are housable; a sealing lid housing part 25.sub.i in which one or two or more sealing lids 251.sub.i, which have transparency, that are detachably mounted on the nozzles are housed or are housable; and housing parts for tips and the like 21.sub.i that house, a plurality of dispensing tips 211.sub.i that are detachably mounted on the nozzles, and the samples, and the like. The liquid housing part group 27.sub.i has, at the very least, one or two or more liquid housing parts that house a magnetic particle suspension, and two or more liquid housing parts that house a solution for separating and extracting used for the separation and the extraction of nucleic acids or the fragments thereof If necessary, it further has two or more liquid housing parts that house a solution for amplification used for the amplification of nucleic acids, and a liquid housing part that houses a sealing liquid for sealing the solution for amplification housed in a PCR tube 231.sub.i, which represents the reaction vessel, within the PCR tube 231.sub.i.

(45) It is preferable for the exclusive regions 20.sub.i to display a barcode as the sample information and the inspection information for identifying the exclusive regions 20.sub.i. Furthermore, the nozzle head 50 is provided with a single traversable nozzle 71.sub.0 in which liquids are transportable or dispensable by traversing (moving in the Y axis direction) the exclusive regions 20.sub.i, and suction and discharge is made to be performed by a traversable nozzle suction-discharge mechanism 17 that is separate from the suction-discharge mechanism 53. Consequently, the solution of DNA and the like housed in a given exclusive region 20.sub.i can be dispensed or delivered to the other exclusive regions 20.sub.k (ki). It is preferable for this movement in the Y axis direction to be also used by the arranging body Y axis transfer mechanism 41.

(46) The CPU+program 60 has, at the very least: a nucleic acid processing control portion 63 that performs instructions for a series of processes, such as extraction and amplification with respect to nucleic acids or the fragments thereof, and sealing of the solution for amplification, with respect to the temperature controller 29, the nozzle head transfer mechanism 51, the tip detaching mechanism 59, the suction-discharge mechanism 53, the magnetic force part 57, and the nozzle Z axis transfer mechanism 75; and a measurement control portion 61 that, after the nozzle head transfer mechanism 51 and the stage Z axis transfer mechanism 35 are controlled such that the linking portions 31, are simultaneously directly or indirectly linked with the apertures of the plurality (twelve in this example) of PCR tubes 231.sub.i, instructs a measurement by the measurement devices 40.sub.j by controlling the arranging body Y axis transfer mechanism 41 such that the optical fibers (bundle) 33.sub.i, which represent the light guide portions of the linking portions 31.sub.i, and the first measuring ends 42.sub.j and the second measuring ends 43.sub.j of the measuring ends 44.sub.j of the measurement devices 40.sub.j mentioned below are optically connected.

(47) Furthermore, the nucleic acid processing control portion 63 has an extraction control part 65 and a sealing lid control part 67. The nucleic acid processing control portion 63 has the extraction control part 65 that performs instructions with respect to the tip detaching mechanism 59, the suction-discharge mechanism 53, the magnetic force part 57, the nozzle Z axis transfer mechanism 75, the nozzle head transfer mechanism 51, and the stage Z axis transfer mechanism 35, for a series of processes with respect to the nucleic acids or the fragments thereof, and the sealing lid control part 67 that performs instructions with respect to the stage Z axis transfer mechanism 35 and the nozzle head transfer mechanism 51 for a sealing process by the sealing lids.

(48) Hereunder, a more specific first embodiment of the light measurement apparatus for a reaction vessel 10 mentioned above according to an embodiment of the present invention, is described with reference to FIG. 2 to FIG. 10. FIG. 2 is a see-through perspective view showing an external view of the light measurement apparatus for a reaction vessel 10 according to the first embodiment of the present invention.

(49) FIG. 2A is a drawing showing an external view of the light measurement apparatus for a reaction vessel 10, which has: an enclosure 11 with a size of 500 mm in depth (Y axis direction), 600 mm in width (X axis direction), and 600 mm in height (Z axis direction) for example, in which the vessel group 20, the nozzle head a nozzle head transfer mechanism 51 described in FIG. 1, and a CPU+program 60 are housed in the interior; a control panel 13 provided on the enclosure 11; and a drawer 15 to which a stage is provided.

(50) FIG. 2B is a perspective view that sees through the interior of the enclosure 11, wherein the stage, into which the vessel group 20 is built-in, is able to be drawn out to the exterior by means of the drawer 15, and further, the nozzle head 50 is movably provided in the X axis direction with respect to the vessel group 20 by means of the nozzle head transfer mechanism 51 of FIG. 1.

(51) FIG. 2B is a drawing showing that the nozzle head 50 is largely provided with: various transfer mechanisms 52 having an arranging body Y axis transfer mechanism 41, a stage Z axis transfer mechanism 35, and a nozzle Z axis transfer mechanism 75; a traversable nozzle suction-discharge mechanism 17; the measuring device 40; a connecting end arranging body 30; an optical fiber (bundle) 33.sub.i; and the magnetic force part 57. The traversable nozzle suction-discharge mechanism 17 and the traversable nozzles 71.sub.0 are supported such that they are movable in the Y axis direction by means of the arranging body Y axis transfer mechanism 41 such that they traverse the exclusive regions 20.sub.i.

(52) FIG. 3 is a plan view showing enlarged, the vessel group 20 shown in FIG. 2. The vessel group 20 is one in which twelve exclusive regions 20i (i=1, . . . , 12), wherein the longitudinal direction thereof is along the X axis direction and housing parts are arranged in a single row form, are arranged in parallel along the Y axis direction at a pitch of 18 mm for example. The exclusive regions 20.sub.i are separately provided with a cartridge vessel for PCR amplification 201.sub.i, a cartridge vessel for nucleic acid extraction 202.sub.i, and a cartridge vessel for housing tips 203.sub.i. The prevention of cross-contaminations between the exclusive regions 20.sub.i is achieved by providing partition walls 201.sub.0, 202.sub.0, and 203.sub.0 on the cartridge vessels 201.sub.i, 202.sub.i, and 203.sub.i of the exclusive regions 20.sub.i on the edge of one side along the X axis direction.

(53) The cartridge vessel for PCR amplification 201.sub.1 has: the PCR tubes 231.sub.1, which represent the reaction vessel that are detachably linked with the twelve linking portions 31.sub.i provided to the light guide stage 32, via a single sealing lid 251.sub.i which has transparency; the liquid housing parts 271.sub.i which house a buffer solution necessary for the PCR reaction; the sealing lid housing parts 25.sub.i which house the sealing lids 251.sub.i; the housing part for tips and the like 21.sub.i that house the tips for punching for punching the film covering the PCR tubes 231.sub.i and the liquid housing parts 271.sub.i, and the dispensing tips 211.sub.i, and barcodes 81.sub.i that display the sample information and the inspection information relating to the cartridge vessels for PCR amplification 201.sub.i.

(54) The cartridge vessels for nucleic acid extraction 202.sub.i has: seven liquid housing parts 272.sub.i for example, that house various reagents for nucleic acid extraction; reaction vessels 232.sub.i that house the extracted nucleic acids; and barcodes 82.sub.i that display various information, such as the sample information and the inspection information, related to the cartridge vessel. The PCR tubes 231.sub.i and the reaction vessels 232.sub.i are temperature controllable by means of the temperature controller 29.

(55) The cartridge vessels for housing tips 203.sub.i has: a tip for punching that is able to punch the film covering the cartridge vessel for nucleic acid extraction 202.sub.i; two small-quantity dispensing tips that perform the dispensing of small quantities of liquids; housing parts for tips and the like 21.sub.i that house dispensing tips for separations that are able to perform separation by adsorbing magnetic particles on an inner wall by applying and removing a magnetic force from the exterior, and a barcode 83.sub.i that displays various information relating to the cartridge vessel 203.sub.i.

(56) The capacity of the PCR tube 231.sub.i, which represents the reaction vessel, is of the order of approximately 200 L, and the capacity of the other reaction vessels, liquid housing parts, and tubes is of the order of approximately 2 mL.

(57) The PCR tube 231.sub.i is used for the amplification of nucleic acids or the fragments thereof, and temperature control is performed by means of the temperature controller 29 based on a predetermined amplification method, such as a thermal cycle (from 4 C. to 95 C.) for example. The PCR tube 231.sub.i is formed with two levels as shown in FIG. 9 for example, and has a narrow-mouthed piping part 233 provided on the lower side in which the solution for amplification 234.sub.i is housed, and a wide-mouthed piping part 235.sub.i provided on the upper side in which the sealing lid 251.sub.i is fittable. The inner diameter of the wide-mouthed piping part 235.sub.i is 8 mm for example, and the inner diameter of the aperture of the narrow-mouthed piping part 233.sub.i is approximately 5 mm for example. The reaction vessels 232.sub.i housed in the reaction tube housing holes are temperature controlled for incubation to a constant temperature of 55 C. for example.

(58) The liquid housing part group 272.sub.i houses the solutions for separating and extracting as follows. A first liquid housing part houses 40 L of Lysis 1, a second liquid housing part houses 200 L of Lysis 2, a third liquid housing part houses 500 L of a binding buffer solution, a fourth liquid housing part houses a magnetic particle suspension, a fifth liquid housing part houses 700 L of a washing liquid 1, a sixth liquid housing part houses 700 L of a washing liquid 2, a seventh liquid housing part houses 50 L of distilled water as a dissociation liquid, and an eighth liquid housing part, which is slightly separated, houses 1300 L of isopropyl alcohol (isopropanol) used for the removal of protein and the like, as a portion of the solution for separating and extracting protein. The respective reagents and the like are prepacked as a result of the punchable film covering the respective apertures thereof.

(59) In addition, 1.2 mL of distilled water is housed in a separate distilled water reservoir, and tubes that house suspensions of bacteria, cells, and the like, or samples such as whole blood, are separately prepared for each of the respective exclusive regions 20.sub.i.

(60) FIG. 4 is a front view and a side view of the nozzle head 50 according to the first embodiment of the present invention, and FIG. 5 is a perspective view from the front side.

(61) The nozzle head 50 is one having: a nozzle arranging portion 70 in which twelve nozzles 71.sub.i are arranged; a tip detaching mechanism 59 that is able to detach dispensing tips 211.sub.i mounted on the nozzles 71.sub.i; a suction-discharge mechanism 53; a magnetic force part 57 having twelve magnets 571 provided such that they are able to approach and separate with respect to the dispensing tips 211.sub.i; a light guide stage 32; twelve linking portions 31.sub.i provided to the light guide stage 32; a transfer mechanism portion 52 having a nozzle Z axis transfer mechanism 75 and a stage Z axis transfer mechanism 35; optical fibers (bundles) 33.sub.i representing flexible light guide portions that extend to the rear side from the linking portions 31.sub.i; a connecting end arranging body 30; the arranging body Y axis transfer mechanism 41; a measuring device 40 having a measuring end 44; a traversable nozzle 71.sub.0; and a suction-discharge mechanism 17 thereof.

(62) The nozzle arranging portion 70 is provided with a cylinder supporting member 73 that supports twelve cylinders 531.sub.i such that they are arranged along the Y axis direction at a predetermined pitch of 18 mm for example. The nozzles 71.sub.i are provided on the downward end of the cylinders 531.sub.i such that they are communicated with the cylinders 531.sub.i.

(63) The tip detaching mechanism 59 is provided with detaching shafts 593 on both sides, and has a tip detaching member 591 that detaches the twelve dispensing tips 211.sub.i from the nozzles 71.sub.i by sliding in the vertical direction.

(64) As shown specifically in FIG. 6 and FIG. 7, the tip detaching member 591 is interlocked with the lowering of two tip detaching shafts 593 and detaches the dispensing tips 211.sub.i from the nozzles 71.sub.i. The tip detaching shaft 593 is elastically supported by the cylinder support member 73 by means of a spring 600 wrapped around the outer periphery such that it is biased in the upward direction, and the upper end thereof is positioned above the upper ends of the cylinders 531.sub.i but below the lower limit position of the vertical movement range of the normal suction and discharge of a cylinder drive plate 536 mentioned below. The two tip detaching shafts 593 are pushed in the downward direction by means of the cylinder drive plate 536 exceeding the vertical movement range and being lowered near the upper end of the cylinder 531.sub.i, thus lowering the tip detaching member 591. The tip detaching member 591 has twelve holes having an inner diameter that is larger than the outer diameter of the nozzles 71.sub.i but smaller than the mounting portions 211.sub.ic, which represents the largest outer diameter of the dispensing tips 211.sub.i, arranged at the pitch mentioned above such that the nozzles 71.sub.i pass therethrough.

(65) As shown specifically in FIG. 6 and FIG. 7, the suction-discharge mechanism 53 has: the cylinders 531.sub.i for performing suction and discharge of gases with respect to the dispensing tips 211.sub.i which are communicated with the nozzles 71.sub.i and mounted on the nozzles 71.sub.i, and a piston rod 532 that slides within the cylinders 531.sub.i; a drive plate 536 that drives the piston rod 532; a ball screw 533 that threads with the drive plate 536; a nozzle Z axis movable body 535 that, in addition to axially supporting the ball screw 533, is integrally formed with the cylinder support member 73; and a motor 534 mounted on the nozzle Z axis movable body 535 that rotatingly drives the ball screw 533.

(66) The magnetic force part 57 has a magnet 571 that is provided such that it can approach and separate with respect to the narrow diameter portions 211.sub.ia of the dispensing tips 211.sub.i detachably mounted on the nozzles 71.sub.i, and is able to apply and remove a magnetic field in the interior of the dispensing tips 211.sub.i.

(67) As shown specifically in FIG. 6, the nozzle Z axis transfer mechanism 75 has: a ball screw 752 that threads with the Z axis movable body 535 and vertically moves the Z axis movable body 535 along the Z direction; a nozzle head substrate 753 that axially supports the ball screw 752, and in addition to axially supporting the magnet 571 on the lower side thereof such that it is movable in the X axis direction, is itself movable in the X axis direction by means of the nozzle head transfer mechanism 51 mentioned below; and a motor 751 provided on the upper side of the nozzle head substrate 753 that rotatingly drives the ball screw 752.

(68) As shown specifically in FIG. 6, the light guide stage 32 comprises a horizontal plate 32a and a vertical plate 32b, which are letter-L shaped plates in cross-section, and is provided with twelve cylinder-shaped linking portions 31.sub.i having front ends of optical fibers (bundles) 33.sub.i, which are directly or indirectly linkable with the apertures of the PCR tubes 231.sub.i and are optically connected with the interior of the linked PCR tubes 231.sub.i, protruding in the downward direction from the horizontal plate 32a. Furthermore, a heater 37 that heats the sealing lids 251.sub.i mounted on the linking portions 31.sub.i and prevents condensation, is built into the bases of the linking portions 31.sub.i. The temperature of the heater is set to approximately 105 C. for example. Since the light guide stage 32 is supported by the nozzle head substrate 753 by means of the nozzle head stage Z axis transfer mechanism 35 such that it is movable in the Z axis direction, it is movable in the nozzle X axis direction and Z axis direction.

(69) The stage Z axis transfer mechanism 35 has: a side plate 355 provided on the nozzle head substrate 753; a mount driving band-shaped member 354 that is supported by a timing belt 352 spanning between two sprockets 353 arranged in the vertical direction axially supported by the side plate 355, and vertically moves in the Z axis direction; and a motor attached to the rear side of the nozzle head substrate 753 that rotatingly drives the sprockets 353.

(70) As shown in FIG. 7, the traversable nozzle suction-discharge mechanism 17 is provided with a tip detaching mechanism 592 on the lower side of the suction-discharge mechanism 17 and on the upper side of the nozzle 71.sub.0. Furthermore, the suction-discharge mechanism 17 is provided with a digital camera 19. The suction-discharge mechanism 17 is movably provided in the Y axis direction by being attached to a timing belt 171 spanning between two sprockets 173 that are rotatingly driven by a motor 172.

(71) FIG. 8 represents two perspective views of the nozzle head according to the first embodiment viewed from the rear side, which show the connection starting position (FIG. 8A) and the connection finishing position (FIG. 8B) at the time the respective connecting ends of the connecting end arranging body 30 and the respective measuring ends are successively optically connected.

(72) There are provided a connecting end arranging body 30 in which the connecting ends 34, provided corresponding to the respective linking portions 31, to which the front ends of the optical fibers (bundles) 33.sub.i which pass through the horizontal plate 32a of the light guide stage 32, are provided, and provided with the back ends thereof, are arranged on an arranging surface on a path along a straight line in the Y axis direction, which represents a predetermined path, at a shorter spacing than the spacing of the linking portions 31.sub.i; and six measuring ends that are provided in the vicinity of, or making contact with, the arranging surface, and are successively optically connectable with the connecting ends 34.sub.i along the straight line. There is also provided a measuring device 40 in which, by means of optical connections between the connecting ends and the measuring ends, the fluorescent light within the PCR tubes 231.sub.i, which represents the optical state, is receivable, and excitation light is also able to be irradiated.

(73) Furthermore, the light guide stage 32 has a cylinder-shaped body 311.sub.i, which retains the optical fibers (bundles) 33.sub.i extending to the rear side from the linking portions 31.sub.i such that they pass through the interior in order to prevent folding, protrudingly provided upward from the horizontal plate 32a directly above the linking portions 31.sub.i. In the same manner, the connecting end arranging body 30 is also provided with a cylinder-shaped body 301.sub.i, which retains the optical fibers (bundles) 33.sub.i extending from the connecting ends 34.sub.i such that they pass through the interior in order to prevent folding, on the connecting end 34.sub.i side.

(74) The arranging body Y axis transfer mechanism 41 that moves the connecting end arranging body 30 in the Y axis direction has: arms 412 and 413 provided to the connecting end arranging body 30; a joining body 411 that joins the arms 412 and 413 and the timing belt; a guide rail 414 that guides the Y axis movement of the joining body 411; and two sprockets spanned by the timing belt and arranged along the Y axis direction.

(75) The measuring device 40 is one that supports the measurement of fluorescent light and comprises six types of specific wavelength measuring devices 40.sub.j that are linearly aligned along a straight line in the Y axis direction, which represents the predetermined path, such that they support the measurement of six types of fluorescent light, and they are provided fixed on a substrate of the nozzle head 50, such as the frame that encloses the transfer mechanism portion 52, or a member that supports the same. Therefore, depending on the mechanism provided to the transfer mechanism portion 52, the measuring device 40 does not move.

(76) The measuring device 40 is one in which the measuring ends of the plurality of types (six in this example) of specific wavelength measuring devices 40.sub.j (j=1, 2, 3, 4, 5, 6), and therefore, in this case, the specific wavelength measuring devices 40.sub.j themselves are aligned in a single row form, and integrally fixed to a member joined with the nozzle head substrate 753 using fixtures 45.sub.j. The specific wavelength measuring devices 40.sub.j have: measuring ends 44.sub.j arranged along a straight line path in the Y axis direction which represents the predetermined path, such that they successively optically connect to the connecting ends 34.sub.i; light detectors 46.sub.j in which an optical system component having an irradiation source that irradiates excitation light to the PCR tubes 231.sub.i and a light receiving portion that receives the fluorescent light generated in the PCR tubes 231.sub.i are built-in; and circuit boards 47.sub.j. The measuring ends 44.sub.j have first measuring ends 42.sub.j that optically connect with the irradiation source, and second measuring ends 43.sub.j that optically connect with the light receiving portion. Here, the light detectors 46.sub.j and the circuit boards 47.sub.j correspond to the measuring device main body.

(77) The pitch between the respective connecting ends 34.sub.j, assuming a pitch between the linking portions 31.sub.i of 18 mm, is 9 mm, which is half thereof. Then, the pitch between the measuring ends 44.sub.j is 9 mm or less for example.

(78) There is a case where the first measuring ends 42.sub.j and the second measuring ends 43.sub.j of the measuring ends 44.sub.j of the respective specific wavelength measuring devices 40j are arranged aligned in a lateral direction (Y axis direction) along the straight line of the Y axis direction along the predetermined path, and a case where they are arranged aligned in a longitudinal direction (X axis direction). In the former case, without stopping the emission of the excitation light, the respective measuring devices successively receive light at a timing for receiving light determined based on the speed of the connecting end arranging body, the pitch between the connecting ends, the distance between the first measuring ends and the second measuring ends of the measuring ends, and the pitch between the measuring ends.

(79) On the other hand, in the latter case, as shown in FIG. 8, with respect to the connecting end, a first connecting end and a second connecting end are provided. The first connecting end connects only with the first measuring ends 42.sub.j, and the second measuring ends 43.sub.j connect only with the second connecting end. The fixed path represents two paths. Furthermore, the optical fibers (bundles) 33.sub.i have optical fibers (bundles) 331.sub.i for receiving light that have the first connecting end, and optical fibers (bundles) 332.sub.i for irradiation that have the second connecting end. In this case, compared to the former case, connection with the linking portions is performed by means of optical fibers in which the irradiation source and the light receiving portion are dedicated, and therefore, the control is simple, and the reliability is high since optical fibers that are respectively suitable for irradiation and receiving light can be used.

(80) The speed of the connecting end arranging body 30 with respect to the measuring ends 44.sub.j is determined with consideration of the stable light receivable time, the lifetime of the fluorescent light with respect to excitation light irradiation, the number of connecting ends, the pitch between the connecting ends, and the like (the distance of the predetermined path). In the case of a real-time PCR measurement, it is controlled such that it becomes 100 mm to 500 mm per second for example. In the present embodiment, since the movement is performed by sliding the arranging surface with respect to the measuring ends 44, the incidence of optical noise to the measuring ends 44 can be prevented. Furthermore, the connecting end arranging body 30 moves with respect to the measuring ends intermittently such that it momentarily stops at each pitch advance between the connecting ends or between the measuring ends, or continuously.

(81) FIG. 9A is a drawing showing a state in which the linking portion 31i (here, i=1 for example) that protrudes on the lower side from the horizontal plate 32a of the light guide stage 32 is indirectly linked with the PCR tube 231i via the sealing lid 251i, which has transparency, that is mounted on the aperture of the PCR tube 231i in the exclusive region 20i, and the linking portion 31i is inserted within the indentation of the sealing lid 251i, and the end surface thereof is adhered to the bottom surface of the indentation of the sealing lid 251i. The PCR tube 231i comprises a wide-mouthed piping part 235i and a narrow-mouthed piping part 233i that is communicated with the wide-mouthed piping part 235i and is formed narrower than the wide-mouthed piping part 235i. Furthermore, the narrow-mouthed piping part 233i is dried beforehand, or a liquid state solution for amplification 234i is housed beforehand. Here, the reagent for real-time amplification represents 70 L, of a master mix (SYBR (registered trademark) Green Mix) consisting of enzymes, buffers, primers, and the like.

(82) For the aperture of the wide-mouthed piping part 235i, since the sealing lid 251i that protrudes on the lower side of the sealing lid 251i, which has transparency, is mounted on the reaction vessel, it is mounted to the reaction vessel as a result of a tubular sealing portion 252i, which encloses the center portion of the sealing lid 251i in which light passes through, being fitted. At the time the sealing portion 252i is fitted, it is preferable for the diameter of the optical fibers (bundle) 33i, which represents the light guide portion that passes through the interior of the linking portion 31i, to be the same or larger than the diameter of the aperture of the narrow-mouthed piping part 233i. Consequently, it becomes possible to receive the light from the PCR tube 231i with certainty. The narrow-mouthed piping part 233i is housed within a block for temperature control that is heated or cooled by means of the temperature controller 29.

(83) In this example, the optical fibers (bundle) 33i comprise optical fibers (bundle) for irradiation 332i that are connectable with the second measuring end 43j and optical fibers (bundle) for receiving light 331i that are connectable with the first measuring end 42j.

(84) FIG. 9B is a drawing showing an example in which the optical fibers (bundle) 33i comprise an optical fiber bundle in which an optical fiber bundle comprising a plurality of optical fibers for receiving light that are connectable with the second measuring end 43j, and an optical fiber bundle comprising a plurality of optical fibers for irradiation that are connectable with the first measuring end 42j, are combined such that they become uniform.

(85) It is preferable for a feeding device for samples and the like to be integrated to the light measurement apparatus for a reaction vessel 10. The feeding device for samples and the like is a device for dispensing and supplying parent samples and the like with respect to the vessel group 20, and the stage in which is integrated the vessel group 20 to which the parent samples and the like have been supplied, is automatically moved to the light measurement apparatus for a reaction vessel and the like. The feeding device for samples and the like, for example, has a parent vessel group that houses parent samples and the like, a tip detaching mechanism, a suction-discharge mechanism, and a single nozzle that, in addition to the suction and the discharge of gases being performed by means of the mechanisms, is detachably mounted with dispensing tips 211i. Furthermore, it has a nozzle head provided with a mechanism that moves along the Z axis direction with respect to the parent vessel group and the housing part group for tips and the like 21 of the vessel group 20, an X axis movable body provided with a Y axis transfer mechanism that moves the nozzle head in the Y axis direction with respect to the parent vessel group and the like, an X axis transfer mechanism that moves the X axis movable body along the X axis with respect to the parent vessel group and the like, and the parent vessel group. It is preferable for the parent vessel group to have a parent sample housing part group arranged in a 12 row8 column matrix form that houses the parent samples to be supplied to the housing part group for tips and the like 21 of the vessel group 20, a distilled water and washing liquid group, and a reagent bottle group.

(86) FIG. 10 is a drawing showing a light detector 46.sub.1 of a single specific wavelength measurement device 40.sub.1 belonging to the measurement device 40 according to a first exemplary embodiment of the present invention.

(87) The specific wavelength measurement device 40.sub.1 according to the present exemplary embodiment, in addition to having an optical fiber 469 for excitation light to be outgoing to the PCR tube 231i and an optical fiber 479 for light from the PCR tube 231i to be incoming, has a measuring end 441 provided on the lower ends of a first measuring end 421 of the optical fiber 469 and a second measuring end 431 of the optical fiber 479, an irradiation portion 462 that has a LED 467 that irradiates excitation light through the optical fiber 469 and a filter 468, and a light receiving portion that has an optical fiber 479, a drum lens 478, a filter 477, and a photodiode 472. This example shows a case where the first measuring end 421 and the second measuring end 431 are provided along a direction (X axis direction) perpendicular to the straight line of the Y axis direction, which represents the predetermined path.

(88) Next, a series of processing operations that perform real-time PCR of the nucleic acids of a sample containing bacteria using the light measurement apparatus for a reaction vessel 10 according to the embodiment is described. Step S1 to step S13 below correspond to separation and extraction processing.

(89) In step S1, the drawer 15 of the light measurement apparatus for a reaction vessel 10 shown in FIG. 2 is opened, the vessel group 20 is pulled out, and by utilizing a feeding device for samples and the like, which is provided separately from the vessel group 20, the samples which are subject to testing, various washing liquids, and various reagents, are supplied beforehand, and furthermore, a liquid housing part in which reagents and the like are prepacked is mounted.

(90) In step S2, following returning of the vessel group 20 and closing of the drawer 15, the start of the separation and extraction and amplification processing is instructed by means of the operation of the touch panel of the control panel 13 for example.

(91) In step S3, the extraction control part 65 provided to the nucleic acid processing controller 63 of the CPU+program 60 of the light measurement apparatus for a reaction vessel 10 instructs the nozzle head transfer mechanism 51 and moves the nozzle head 50 in the X axis direction, positions the tip for punching mounted to the nozzle 71.sub.i above the first liquid housing part of the liquid housing part group 27.sub.i of the vessel group, and punches the film covering the aperture of the liquid housing part by lowering the nozzle by means of the nozzle Z axis transfer mechanism 75, and in the same manner, the other liquid housing parts of the liquid housing part group 27.sub.i and the reaction vessel group 23.sub.i are successively punched by moving the nozzle head 50 in the X axis direction.

(92) In step S4, the nozzle head 50 is again moved in the X axis direction and moved to the housing part for tips and the like 21.sub.i, and the nozzles 71.sub.i are lowered by means of the nozzle Z axis transfer mechanism 75, and the dispensing tips 211.sub.i are mounted. Next, after being raised by the nozzle Z axis transfer mechanism 75, the dispensing tips 211.sub.i are moved along the X axis by means of the nozzle head transfer mechanism 51, and advanced to the eighth liquid housing part of the liquid housing part group 27.sub.i. Then a predetermined amount of isopropanol is aspirated from the liquid housing part, and they are again moved along the X axis direction, and predetermined amounts are respectively dispensed into the solution components (NaCl, SDS solutions) housed in the third liquid housing part and the fifth liquid housing part, and the distilled water housed in the sixth liquid housing part, so that 500 L of a binding buffer solution (NaCl, SDS, isopropanol), 700 L of a washing liquid 1 (NaCl, SDS, isopropanol), and 700 L of a washing liquid 2 (water 50%, isopropanol 50%) are respectively prepared as solutions for separating and extracting within the third, the fifth, and the sixth liquid housing parts.

(93) In step S5, following movement to, among the housing parts for tips and the like 21.sub.i, the sample tube in which the sample is separately housed, the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is loweringly inserted using the nozzle Z axis transfer mechanism 75, and, with respect to the suspension of the sample housed in the sample tube, following suspension of the sample within the liquid by repeating the suction and the discharge by raising and lowering the drive plate 536 of the suction-discharge mechanism 53, the sample suspension is aspirated within the dispensing tip 211.sub.i. The sample suspension is moved along the X axis by means of the nozzle head transfer mechanism 51 to the first liquid housing part of the liquid housing part group 27.sub.i housing the Lysis 1 (enzyme) representing the solution for separating and extracting, and the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is inserted through the hole in the punched film, and the suction and the discharge is repeated in order to stir the sample suspension and the Lysis 1.

(94) In step S6, the entire amount of the stirred liquid is aspirated by the dispensing tip 211.sub.i, and incubation is performed by housing it in the reaction vessel 232.sub.i comprising the reaction tubes retained in the housing holes, that is set to 55 C. by means of the constant temperature controller. Consequently, the protein contained in the sample is broken down and made a low molecular weight. After a predetermined time has elapsed, the reaction mixture is left in the reaction tube, the dispensing tip 211.sub.i is moved to the second liquid housing part of the liquid housing part group 27.sub.i by means of the nozzle head transfer mechanism 51, and the entire amount of the liquid housed within the second liquid housing part is aspirated by using the nozzle Z axis transfer mechanism 75 and the suction-discharge mechanism 53, and it is transferred using the dispensing tip 211.sub.i by means of the nozzle head transfer mechanism 51, and the reaction solution is discharged within the third liquid housing part by penetrating the hole in the film and inserting the narrow diameter portion.

(95) In step S7, the binding buffer solution housed within the third liquid housing part, which represents a separation and extraction solution, and the reaction solution are stirred, the solubilized protein is further dehydrated, and the nucleic acids or the fragments thereof are dispersed within the solution.

(96) In step S8, using the dispensing tip 211.sub.i, the narrow diameter portion thereof is inserted into the third liquid housing part by passing through the hole in the film, the entire amount is aspirated and the dispensing tip 211.sub.i is raised by means of the nozzle Z axis transfer mechanism 75, and the reaction solution is transferred to the fourth liquid housing part, and the magnetic particle suspension housed within the fourth liquid housing part is stirred with the reaction solution. A cation structure in which Na+ ions bind to the hydroxyl groups formed on the surface of the magnetic particles contained within the magnetic particle suspension is formed. Consequently, the negatively charged DNA is captured by the magnetic particles.

(97) In step S9, the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i by approaching the magnet 571 of the magnetic force part 57 to the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i. In a state in which the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, the dispensing tip 211.sub.i is raised by means of the nozzle Z axis transfer mechanism 75 and moved from the fourth liquid housing part to the fifth liquid housing part using the nozzle head transfer mechanism 51, and the narrow diameter portion 211.sub.ia is inserted by passing through the hole in the film.

(98) In a state in which the magnetic force within the narrow diameter portion 211.sub.ia is removed by separating the magnet 571 of the magnetic force part 57 from the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, by repeating the suction and the discharge of the washing liquid 1 (NaCl, SDS, isopropanol) housed in the fifth liquid housing part, the magnetic particles are released from the inner wall, and the protein is washed by stirring within the washing liquid 1. Thereafter, in a state in which the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia as a result of approaching the magnet 571 of the magnetic force part 57 to the narrow diameter portion 211.sub.ia of the narrow diameter portion 211.sub.ia again, the dispensing tip 211.sub.i is, by means of the nozzle Z axis transfer mechanism 75, moved from the fifth liquid housing part to the sixth liquid housing part by means of the nozzle head transfer mechanism 51.

(99) In step S10, the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is inserted by passing through the hole in the film using the nozzle Z axis transfer mechanism 75. By repeating the suction and the discharge of the washing liquid 2 (isopropanol) housed in the sixth liquid housing part in a state in which the magnetic force within the narrow diameter portion 211.sub.ia is removed by separating the magnet 571 of the magnetic force part 57 from the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, the magnetic particles are stirred within the liquid, the NaCl and the SDS is removed, and the protein is washed. Thereafter, in a state in which the magnetic particles are adsorbed on the inner wall of the narrow diameter portion 211.sub.ia by approaching the magnet 571 of the magnetic force part 57 to the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i again, the dispensing tip 211.sub.i is, following raising by means of the nozzle Z axis transfer mechanism 75, moved from the sixth liquid housing part to the seventh liquid housing part in which the distilled water is housed, by means of the nozzle head transfer mechanism 51.

(100) In step S11, the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i is lowered through the hole by means of the nozzle Z axis transfer mechanism 75, and by repeating the suction and the discharge of the distilled water at a slow flow rate in a state where the magnetic force is applied within the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, the washing liquid 2 (isopropanol) is substituted by water and is removed. Thereafter, by stirring the magnetic particles by repeating the suction and the discharge within the distilled water which represents the dissociation liquid, in a state in which the magnet 571 of the magnetic force part 57 is separated from the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i and the magnetic force is removed, the nucleic acids or the fragments thereof retained by the magnetic particles are dissociated (eluted) from the magnetic particles into the liquid. Thereafter, a magnetic field is applied within the narrow diameter portion and the magnetic particles are adsorbed on the inner wall by approaching the magnet 571 to the narrow diameter portion 211.sub.ia of the dispensing tip 211.sub.i, and the solution containing the extracted nucleic acids, and the like, is made to remain in the eighth liquid housing part. The dispensing tip 211.sub.i is moved to the storage part of the housing parts for tips and the like 21.sub.i in which the dispensing tip 211.sub.i was housed, by means of the nozzle head transfer mechanism 51, and the dispensing tip 211.sub.i to which magnetic particles are adsorbed, is detached from the nozzle 71.sub.i together with the magnetic particles and dropped into the storage part, using the detaching member 591 of the tip detaching mechanism 59.

(101) The following step S12 to step S15 corresponds to nucleic acid amplification and measurement processing.

(102) In step S12, a new dispensing tip 211.sub.i is mounted on the nozzle 71.sub.i, the solution housed within the eighth liquid housing part, which contains nucleic acids and the like, is aspirated, and by transferring it to the PCR tube 231.sub.i in which the solution for amplification 234.sub.i is housed beforehand, and discharging it, it is introduced into the vessel. As a result of moving the nozzle head 50 by means of the nozzle head transfer mechanism 51, the nozzle 71.sub.i is moved above the sealing lid housing part 25.sub.i of the vessel group 20, which houses the sealing lid 251.sub.i. Mounting is performed by lowering using the nozzle Z axis transfer mechanism 75 and fitting an indentation for linking 258.sub.i on the upper side of the sealing lid 251 to the lower end of the nozzle 71.sub.i. After being raised by the nozzle Z axis transfer mechanism 75, the sealing lid 251 is positioned above the PCR tube 231.sub.i using the nozzle head transfer mechanism 51, and by lowering the sealing lid 234.sub.i by means of the nozzle Z axis transfer mechanism 75, it is fitted with the aperture of the wide-mouthed piping part 235.sub.i of the PCR tube 231.sub.i, mountingly sealing it.

(103) In step S13, the nozzle head transfer mechanism 51 is instructed by means of an instruction from the measurement control portion 61, and by moving the nozzle head 50 along the X axis, the linking portion 31.sub.i of the light guide stage 32 is positioned above the PCR tube 231.sub.i, which is mounted with the sealing lid 251.sub.i. Then, by lowering the light guide stage 32 by means of the stage Z axis transfer mechanism 35, the linking portion 31.sub.i is inserted into the indentation of the sealing lid 251.sub.i, and the lower end thereof is made to make contact with, or adhere to, the bottom surface of the indentation.

(104) In step S14, due to an instruction by the nucleic acid processing controller 63, the temperature controller 29 instructs a temperature control cycle by real-time PCR, such as a cycle in which the PCR tube 231.sub.i is heated for five seconds at 96 C. and heated for 15 seconds at 60 C., to be repeated forty nine times for example.

(105) In step S15, when temperature control at each cycle by the nucleic acid processing controller 63 is started, the measurement control portion 61 determines the start of elongation reaction processing at each cycle, and instructs the continuous or intermittent movement of the connecting end arranging body 30 with respect to the measuring ends 44.sub.j of the measuring device 40. For the movement speed thereof, it is moved at a speed that is calculated based on the stable light receivable time, the fluorescence lifetime, and the number (twelve in this example) of exclusive regions 20.sub.i. Consequently, the receiving of light from all twelve PCR tubes 231.sub.i within the stable light receivable time becomes completed.

(106) In step S16, the measurement control portion 61 determines the moment of each optical connection between the optical fibers (bundles) 33.sub.i of the linking portions 31.sub.i and the first measuring end and the second measuring end of the measuring end 44, and instructs the receiving of light to the measuring device 40 for example.

(107) This measurement is executed with respect to cycles in which exponential amplification is performed, and an amplification curve is obtained based on the measurement, and various analyses are performed based on the amplification curve. At the time of the measurement, the measurement control portion 61 heats the heater 37 built into the light guide stage 32 and prevents the condensation on the sealing lid 251, and a clear measurement can be performed.

(108) FIG. 11 is a perspective view of the front side of a nozzle head 500 of a light measurement apparatus for a reaction vessel according to a second exemplary embodiment of the present invention, and a perspective view showing a portion thereof cut away. FIG. 12 is a perspective view showing enlarged, the portion of FIG. 11 shown cut away.

(109) As shown in FIG. 11, in this example, unlike the light measurement apparatus for a reaction vessel according to the first exemplary embodiment, the PCR tubes 236.sub.i, which represent the reaction vessel, have a vessel group in which three or more rows of twelve each are arranged.

(110) There are no large differences with the first exemplary embodiment with respect to the section of the nozzle head 500 related to a dispensing device, which includes the nozzles, and the section related to the traversable nozzle, the transfer mechanisms of the nozzle head, and the arranging body transfer mechanism, and they are omitted from the descriptions. The nozzle head 500 has: a light guide stage 320; twelve linking portions 310.sub.i provided on the light guide stage 320; optical fibers (bundle) 33.sub.i that extend from the linking portions 310.sub.i on the rear side; a connecting end arranging body 300; a measuring device 400 having a measuring end comprising six types of specific wavelength measurement devices that are aligningly mounted on the light guide stage 320; and a sealing lid transporting body 125.

(111) The light guide stage 320 according to the second exemplary embodiment has a linking portion arranging body 322, in which two or more (twelve in this example) linking portions 310, that are simultaneously linkable with two or more (twelve in this example) PCR tubes 236.sub.i representing reaction vessels, are arranged, that is movable in the horizontal direction (the X axis direction in this example) with respect to the light guide stage 320. Furthermore, by means of the movement of the linking portion arranging body 322, without moving the light guide stage 320, it is linkable with more PCR tubes 236.sub.i representing reaction vessels (three rows of reaction vessels with twelve per row in this example) than the number of reaction vessels (twelve in this example) that are simultaneously linkable by the linking portion arranging body 322.

(112) The light guide stage 320 has a horizontal plate 320a, a vertical plate 320b, and a triangular-shaped support side plate 320c. The horizontal plate 320a of the light guide stage 320, according to the arrangement of the linking portions 310, arranged on the linking portion arranging body 322, is etchingly provided with two or more, or twelve in this example, long holes 321.sub.i that correspond to shielding regions.

(113) The measurement device 400 is mounted fixed to the upper edge of the vertical plate 320b of the light guide stage 320. Therefore, since the light guide stage 320 is stationary at the time of receiving light, the measurement device 400 is immovably provided with respect to the reaction vessel and the light guide stage 320.

(114) The optical fibers (bundle) 33.sub.i having the end of the linking portion 310.sub.i, separate midway into optical fibers for receiving light (bundle) 331.sub.i and optical fibers for irradiation (bundle) 332.sub.i. The optical fibers for receiving light (bundle) 331.sub.i connect to a second connecting end 341.sub.i, and the optical fibers for irradiation (bundle) 332.sub.i connect to a first connecting end 342.sub.i, and are arranged as two paths along the Y axis direction on a downwardly facing horizontal plane, which represents an arranging surface on the lower side of the connecting end arranging body 300. At that time, the spacing between adjacent connecting ends on these respective paths is such that they are integrated at approximately half or one-third of the spacing of the linking portions for example. The first connecting ends 342.sub.i are successively connectable with the first measuring ends of the measurement device 400, and the second connecting ends 341.sub.i are successively connectable with the second measuring ends.

(115) As shown in FIG. 12 or FIG. 13, the horizontal plate 320a of the light guide stage 320 is laminatingly provided with a thermal insulation plate 323 formed by a resin and the like, a heater 370 provided on the lower side of the thermal insulation plate 323 for preventing condensation of the sealing lids 253.sub.i by heating the sealing lids 253.sub.i, and a thermally conductive metallic plate 325 provided on the lower side of the heater 370. Reference symbol 238 is a housing hole that houses the PCR tubes 236.sub.i representing reaction vessels, and is piercingly provided in the cartridge vessel. Reference symbol 239 represents a liquid surface that is controlled at a fixed height within the PCR tubes 236.sub.i representing reaction vessels. Reference symbol 291 is a temperature controller for PCR.

(116) The long holes 321.sub.i that are etchingly provided in the horizontal plate 320a reach the metallic plate 325. Holes 326 for light transmission that are the same size as the apertures are piercingly provided above the apertures of the PCR tubes 236.sub.i representing reaction vessels, of the metallic plate 325 on the bottom of the long holes 321.sub.i, and are optically communicated with the bottom of the long holes 321.sub.i.

(117) The linking portions 310.sub.i provided on the linking portion arranging body and the ends of the optical fibers (bundle) 33.sub.i provided in the interior are, as a result of approaching the sealing lids 253.sub.i, linked with the PCR tubes 236.sub.i representing reaction vessels.

(118) FIG. 14 is a drawing showing the various sealing lids 254.sub.i to 257.sub.i according to the second exemplary embodiment, that are mountable on the reaction vessel.

(119) In FIG. 14A, the sealing lid 253.sub.i has: a cover plate 253.sub.ia that covers the aperture 236.sub.ia of the PCR tube 236.sub.i representing a reaction vessel; a central portion 253.sub.ic that is formed at the center of the cover plate 253.sub.ia and thinner than the periphery, and has an increased light transmittance; and a clamp 253.sub.ib comprising a double annular wall that is provided such that it encloses the central portion 253.sub.ic and protrudes on the lower side, that represents a mounting portion that is mountable to the outer edge portion 236.sub.ib of the aperture of the reaction vessel.

(120) The sealing lid 254.sub.i shown in FIG. 14B is formed thick in a convex lens form having a curved surface that expands from a central portion 254.sub.ic toward the vessel exterior. Consequently, the light that is generated within the reaction vessel is made to converge at the end of an optical fiber, or the excitation light from the optical fiber is made to converge at the liquid surface and the like, and the light can be efficiently collected.

(121) The sealing lid 255.sub.i shown in FIG. 14C is formed in a convex lens form having a curved surface that expands from a central portion 255.sub.ic toward the vessel exterior, and consequently, the effects demonstrated in FIG. 14B are achieved.

(122) The sealing lid 256.sub.i shown in FIG. 14D is formed thick such that it has a curved surface that expands from a central portion 256.sub.ic toward the vessel exterior. Consequently, the effects demonstrated in FIG. 14B are achieved.

(123) The sealing lid 257.sub.i shown in FIG. 14E is formed such that it has a curved surface that expands from a central portion 257.sub.ic toward the vessel exterior, and consequently, the effects demonstrated in FIG. 14B are achieved.

(124) FIG. 15 shows a sealing lid transporting body 125 according to the second exemplary embodiment.

(125) The sealing lid transporting body 125 is one having: a prismatic substrate 128 that is movable in the X axis direction with respect to the vessel group 20, which has at least three rows of PCR tubes 236.sub.i representing reaction vessels of twelve per row; one or two or more (twelve in this example) grippers 127.sub.i arranged on the prismatic substrate 128 according to the arrangement of the reaction vessels that grip the cover plate such that, with respect to the sealing lid 253.sub.i (to 256.sub.i), the lower side is exposed in a state in which the mounting portion is mountable to the reaction vessel; and a bottom plate 126 that is mounted on the lower side of the prismatic substrate 128.

(126) As shown in the cross-sectional view of FIG. 16 and the perspective view as viewed from the lower side of FIG. 17, the grippers 127.sub.i have a cavity 124.sub.i that is cut out from the prismatic substrate 128 in an approximate semicircular column shape such that most of the cover plate 253.sub.ia of the sealing lid 253.sub.i is housable. Furthermore, the bottom plate 126 is provided with a semicircular hole shaped notch portion 129.sub.i such that the clamp 253.sub.ib, which represents the mounting portion of the sealing lid 253.sub.i, is exposable on the lower side.

(127) Next, the processing operation using the nozzle head 500 according to the second exemplary embodiment is described.

(128) Among the processes described in the first exemplary embodiment, the separation and extraction process is omitted, and step S12 to step S16, which correspond to nucleic amplification and measurement processes, are described.

(129) In step S12, a new dispensing tip 211.sub.i is mounted on the nozzle 71.sub.i, the solution containing nucleic acids and the like, which is housed within the eighth liquid housing part is aspirated, transported to the PCR tube 236.sub.i representing a reaction vessel in which the solution for amplification 234.sub.i is housed beforehand, and discharged and introduced into the vessel. As a result of moving the nozzle head 500 by means of the nozzle head transfer mechanism 51, the sealing lids 253.sub.i from the sealing lid housing part in the sealing lid transporting body 125 in which twelve sealing lids 253.sub.i are housed, are simultaneously housed in the cavity 124.sub.i of the grippers 127.sub.i, and gripped.

(130) Since the sealing lid transporting body 125 gripping the sealing lid 253.sub.i is linked with the light guide stage 320, then by using the stage Z axis transfer mechanism 35 and moving it somewhat upwardly together with the stage 320 and then moving it in the X axis direction, and by transporting it to above the PCR tubes 236.sub.irepresenting reaction vessels and lowering it, the twelve sealing lids 253, are sealed by mounting the clamps 253.sub.ib, which are exposed on the lower side from the sealing lid transporting body 125, to the PCR tubes 236.sub.i. In the same manner, the rows of the twenty four reaction vessels of the other two rows are successively sealed by the sealing lids.

(131) In step S13, due to an instruction by the measurement control portion 61, as a result of instructing the nozzle head transfer mechanism 51 and moving the nozzle head 500 along the X axis, the light guide stage 320 is moved such that it covers the thirty six reaction vessels of the three rows, on which the sealing lids are mounted.

(132) In step S14, due to an instruction by the nucleic acid processing control portion 63, the temperature controller 29 instructs a temperature control cycle by real-time PCR, such as a cycle in which the PCR tubes 231.sub.i are heated for five seconds at 96 C. and heated for fifteen seconds at 60 C., to be repeated forty nine times for example.

(133) In step S15, when temperature control at each cycle is started by the nucleic acid processing control portion 63, the measurement control portion 61 determines the start of the elongation reaction process at each cycle, moves the linking portion arranging body 322 provided on the light guide stage 320 over the light guide stage 320, indirectly links the respective linking portions 310.sub.i that are inserted into the long holes 321.sub.i provided on the light guide stage 320 via the reaction vessels and the sealing lids 253.sub.i, and successively receives the light from the reaction vessels while irradiating excitation light from the measurement device to the interior of the reaction vessels. At the same time, the continuous or intermittent movement of the connecting end arranging body 300 with respect to the respective measuring ends 44.sub.j of the measurement device 400 is instructed. The movement speed thereof is such that movement is performed at a speed that is calculated based on the stable light receivable time, the fluorescence lifetime, the number (three rows of twelve reaction vessels per row in this example) of reaction vessels of the exclusive regions 20.sub.i that are measurable by the light guide stage 320, and the like. Consequently, by moving the linking portion arranging body 322 over the light guide stage 320 within the stable light receivable time, in this example, measurements can be performed in parallel with respect to thirty six reaction vessels of three rows, with twelve per row.

(134) In step S16, the measurement control portion 61 determines the moment of the respective optical connections between the optical fibers (bundle) of the linking portions 310.sub.i and the first measuring end and the second measuring end of the measuring end 44, and instructs the irradiation of excitation light and the receiving of light to the measurement device 400.

(135) FIG. 18 is a drawing showing an example of the position of the optical fiber front ends for receiving light and for irradiation provided to the linking portion in a case where the linking portion is linked at a location other than the aperture of the PCR tube 236.sub.i representing a reaction vessel. FIG. 18A is a drawing showing a case where the optical fibers (bundle) for receiving light 331.sub.i are in the vicinity of the outer bottom portion of the reaction vessel, and the optical fibers (bundle) for irradiation 332.sub.i are in the vicinity of the outer wall of the reaction vessel. FIG. 18B is a drawing showing a case where the optical fibers (bundle) for receiving light 331.sub.i and the optical fibers (bundle) for irradiation 332.sub.i are in the vicinity of the outer wall of the reaction vessel. FIG. 18C is a drawing showing a case where the optical fibers (bundle) for receiving light 331.sub.i and the optical fibers (bundle) for irradiation 332.sub.i are in the vicinity of the outer bottom portion of the reaction vessel. These are only examples, and cases where they are joined with the reaction vessel by making contact, and the like, in place of being in the vicinity are also possible.

(136) FIG. 19 represents a block-diagram of a light measurement apparatus for a reaction vessel 110 according to a second embodiment of the present invention. Since the same reference symbols as the reference symbols used in the first embodiment represent the same objects or similar (only differing by size) objects, the descriptions thereof are omitted.

(137) The light measurement apparatus for a reaction vessel 110 according to the second embodiment differs from the light measurement apparatus for a reaction vessel 10 according to the first embodiment in the aspect that the nozzle head 150 thereof has a light guide stage 132 that is different from the light guide stage 32. The light guide stage 132 according to the second embodiment differs from the light guide stage 32 according to the first embodiment in the aspects that it has a plurality (twelve in this example) of linking portions 131.sub.i in which the front ends of optical fibers, which represent two or more light guide portions, which have a flexibility, that optically connect with the interior of the PCR tubes 231.sub.i, and an optical element for collecting light are provided in the interior, and the heat source of the heater 137, which represents a heating portion for heating the reaction vessels, is provided not to the light guide stage 132, but to the vessel group 120 or the stage.

(138) Further, it differs in the aspects that the sealing lids 251.sub.i are transported not by the nozzles 71.sub.i but by fitting to the linking portions 131.sub.i, and are detached from the linking portions by means of a dedicated sealing lid detaching mechanism 39. Therefore, the sealing lid control portion 167, and therefore, the nucleic acid processing control portion 163 and the CPU+program 160 differ from the device 10 according to the first embodiment.

(139) The vessel group 120 is one in which twelve exclusive regions 120.sub.i (i=1, . . . , 12), wherein the longitudinal direction thereof is along the X axis direction and housing parts are arranged in a single row form, are arranged in the Y axis direction for example. The respective exclusive regions 120, have: a reaction vessel group 23.sub.i; a liquid housing part group 27.sub.i; a sealing lid housing part 25.sub.i that houses sealing lids 251.sub.i, which have transparency, that are detachably mounted on the linking portions 131.sub.i provided to the light guide stage 132; and housing parts for tips and the like 21.sub.i.

(140) The reaction vessel 23.sub.i, the temperature controller 29, and the heater 137 correspond to the reaction vessel control system 90.

(141) FIG. 20 is a cross-sectional view primarily showing, within the nozzle head 150 according to a first exemplary embodiment of the second embodiment, the transfer mechanism and the suction-discharge mechanism.

(142) Here, since the diameter of the linking portion 131.sub.i is thicker than the nozzle 71.sub.i, the sealing lid 251.sub.i to be mounted on the PCR tube is transported by the linking portion 131.sub.i. Consequently, by utilizing the transfer mechanism of the magnet 571 of the magnetic force part 57, a sealing lid detaching mechanism 39 is provided that has a comb-shaped detaching member 391 in which a notch portion, which has a semicircular-shaped arch that is approximately equivalent to the diameter of the twelve linking cylinders provided such that they can approach and separate with respect to the linking portion 131.sub.i, is arranged. In the present exemplary embodiment, since the detachment of the sealing lids can be performed by utilizing existing mechanisms, the expansion of the apparatus scale, and increases in complexity, can be prevented.

(143) FIG. 21 is a drawing showing a reaction vessel control system 901 according to the first exemplary embodiment of the second embodiment and a state in which, to the apertures of the reaction vessel group, to which the PCR tubes 231.sub.i representing a plurality (twelve in this example) of reaction vessels of the reaction vessel control system 901 are provided, the linking portions 131, (here, i=1 for example) protruding on the lower side from the horizontal plate 132a of the light guide stage 132 are indirectly linked with the PCR tubes 231i via the sealing lids 251i, which have transparency, that are mounted on the apertures of the PCR tubes 231.sub.i in the exclusive regions 120.sub.i. As a result of the linking portions 131.sub.i fitting within the indentation for linking 253.sub.i of the sealing lids 251.sub.i, they are linked with the PCR tubes 231.sub.i.

(144) As shown in FIG. 21, the linking portion 131.sub.i is indirectly linked with the PCR tube 231.sub.i via the sealing lid 253, and has an approximately cylinder-shaped linking cylinder 131a.sub.i that is protrudingly provided further in the downward direction than the horizontal plate 132a of the light guide stage 132. Furthermore, a circular hole 131b.sub.i having an aperture of a size corresponding to the liquid surface of the liquid that is housed in the narrow-mouthed piping part is piercingly provided in the center portion of the bottom plate of the linking cylinder 131a.sub.i, and the periphery of the bottom plate is provided with a circular edge portion 131d.sub.i that is protrudingly provided below it. Consequently, the adhesion of the linking portion and the sealing lid is prevented. A spherical ball lens 381.sub.i that has a diameter corresponding to the inner diameter of the linking cylinder is loosely inserted within the linking cylinder 131a.sub.i and mounted on the circular hole 131b.sub.i. At a predetermined distance above the ball lens 381.sub.i, an optical fiber 33.sub.i, in which the end is positioned and is covered by a resin-made ferrule 131c.sub.i that passes through the horizontal plate 132a and reaches the exterior, is provided. The linking cylinder 131a.sub.i, the circular hole 131b.sub.i, the ball lens 381.sub.i, and the optical fiber 33.sub.i bundle are arranged on the same axis in the interior of the linking cylinder 131a.sub.i.

(145) As shown in FIG. 21, the reaction vessel control system 901 has: PCR tubes 231.sub.i that represent reaction vessels, in which target solutions of DNA having a target base sequence, and the like, are stored and reactions, such as amplification, are performed; a heater 137; and a temperature controller 291.sub.i for PCR. The heater 137 is laminatingly provided with a heating block 137c comprising an aluminum plate having a high thermal conductivity, a sheet heater 137a, and a heat insulator 137b. Twelve through holes 137d.sub.i that house and retain a plurality (twelve in this example) of PCR tubes 231.sub.i are piercingly provided in the same heater 137, and the wide-mouthed piping parts 235.sub.i are supported by the heating block 137c.

(146) The temperature controller 291 for PCR has: a block for temperature control 292.sub.i that makes contact with, and is housable in, the narrow-mouthed piping part 233.sub.i of the PCR tube 231.sub.i, which represents the reaction vessel; a Peltier element 293.sub.i; and a heat sink 294.sub.i.

(147) The narrow-mouthed piping part 233.sub.i of the PCR tube 231.sub.i has a lower side wall section 233a.sub.i of the section in which the block for PCR 292.sub.i is making contact and is provided. Furthermore, it has an upper side wall section 235a.sub.i provided on the upper side leaving a spacing with the lower side wall section 233a.sub.i that corresponds to the wall section of the wide-mouthed piping part 235.sub.i that makes contact with the block for heating 137c of the heater.

(148) According to the present exemplary embodiment, firstly, by means of an instruction by the sealing lid control portion 167 (the CPU+program 160), the nozzle head transfer mechanism 51 is instructed and following movement of the respective linking portions 131.sub.i of the light guide stage 132 to the sealing lid housing parts 25.sub.i, the stage Z axis transfer mechanism 35 is instructed and the sealing lids 251.sub.i are fitted and mounted to the linking portions 131.sub.i. Next, by fitting the apertures of the predetermined PCR tubes 231.sub.i with the sealing lids 251.sub.i, the linking portions 131.sub.i are simultaneously linked with the PCR tubes 231.sub.i.

(149) Next, according to the temperature control by the temperature controller 29 as a result of an instruction by the measurement control portion 161, in the case of PCR, by controlling the heater 137 such that the upper side wall section 235a.sub.i is heated at a fixed temperature (100 C. for example) that is several degrees, or preferably approximately 5 C., higher than the maximum predetermined temperature (94 C. for example), the sealing lid 251.sub.i fitted to the wide-mouthed piping part 235.sub.i of the PCR tube 231.sub.i is heated, and condensation of the sealing lid can be prevented. At that time, the upper side wall section 235a.sub.i is separated by a fixed spacing from the lower side wall section 233a.sub.i, in which temperature control is performed, and the upper side wall section 235a.sub.i, which has a smaller surface area than the lower side wall section, is heated by bringing the heat source into contact or into its vicinity. Consequently, the effect of heating the upper side walls section 235a.sub.i is such that the lower surface of the sealing lid 251.sub.i, which is provided at a position near the upper side walls section 235a.sub.i, is heated, and condensation can be prevented.

(150) On the other hand, since the linking portion 131.sub.i is only making contact with the upper side of the sealing lid 251.sub.i via the circular edge portion 131d.sub.i, the effect of heating is not as much as with respect to the sealing lid 251.sub.i. In the same manner, the lower side wall section 233a.sub.i is temperature controlled to the predetermined temperature using a Peltier element having a heating and cooling function, and furthermore, measurements are simultaneously performed. After completion of the measurement, then by means of an instruction by the sealing lid control portion 167, the linking portion 131.sub.i is made to approach using the detaching member 391, and then by upwardly moving the light guide stage 132 by means of the stage Z axis transfer mechanism 35, the sealing lid 251.sub.i is detached from the linking portion and while remaining on the PCR tube 231.sub.i, the linking portion is moved and the linking is released.

(151) FIG. 22 is a drawing showing a second exemplary embodiment, and represents a linking portion 131.sub.i in which, in place of the ball lens 381.sub.i, a drum lens 382.sub.i having a lens diameter corresponding to the inner diameter of the linking cylinder 131a.sub.i is loosely inserted within the linking cylinder 131a.sub.i and mounted on the circular hole 131b.sub.i, and is provided such that light is collected at the end of the optical fiber 33.sub.i.

(152) FIG. 23 is a drawing showing a third exemplary embodiment, and represents a linking portion 131.sub.i in which, in place of the ball lens 381.sub.i and the like, an aspheric surface lens 383.sub.i having a lens diameter corresponding to the inner diameter of the linking cylinder 131a.sub.i is loosely inserted within the linking cylinder 131a.sub.i and mounted on the circular hole 131b.sub.i, and is provided such that light is collected at the end of the optical fiber 33.sub.i. Reference symbol 391 represents a comb-shaped detaching member of the sealing lid detaching mechanism 39, and shown is a state in which it is in the vicinity of, or making contact with, the linking portion 131.sub.i. In this state, by raising the linking portion 131.sub.i, the sealing lid 251.sub.i engages with the sealing lid detaching member 391 and is detached from the linking portion 131.sub.i, but remains still mounted on the PCR tube 231.sub.i. Furthermore, the respective lenses 381.sub.i to 383.sub.i may be made to be loosely mounted within the linking cylinder 131a.sub.i by installing a tube-shaped frame from the upper side.

(153) FIG. 24 is a drawing in which PCR tubes 231.sub.i (FIG. 24A) are housed and retained in twelve through holes 137d provided in a heater 137 (FIG. 24B) according to the present embodiment, with the heater 137 then heated to a predetermined temperature. The top surface temperature of the sealing lid is measured at the time the heating temperature of the heater thereof is increased, and the temperature at which condensation occurs, and the temperature at which condensation is eliminated, are measured.

(154) As shown in FIG. 24A, the apertures of the PCR tubes 231.sub.i are sealed by a sealing lid 259, which has a transparency. The diameter of the upper side of the sealing lid is 14 mm, the height of the mounting portion of the sealing lid 259 is 8.6 mm, and the height from the base of the PCR tube to the upper side of the sealing lid 259 is 21.4 mm. FIG. 24C is a drawing showing the actual measured temperature at the top surface of the sealing lid with respect to the set temperature of the heater.

(155) In step S101, 25 L of distilled water is dispensed into the narrow-mouthed piping parts of the respective PCR tubes 231.sub.i, and as shown in FIG. 24A, the apertures of the wide-mouthed piping parts are sealed by the sealing lid 259, and the PCR tubes 231.sub.i are retained in the twelve through holes of the heater 137. The top surface temperature of the sealing lid 259 is measured by opening a small hole (diameter: 0.5 mm) in the sealing lid 259, inserting a thermocouple 900, and installing it on the inside of the top surface of the sealing lid 259.

(156) In step S102, the temperature of blocks for PCR 292.sub.i, which are provided such that the narrow-mouthed piping parts of the PCR tubes 231.sub.i are making contact, are set to 95 C., and the presence of condensation on the top surface of the sealing lid is visually observed. At this time, the set temperature of the heater 137 is gradually raised from 100 C. to 115 C., and the set temperature at which condensation is eliminated is confirmed. Reference symbol 902 represents the position of a thermistor, and a thermocouple is installed in the vicinity thereof.

(157) In a separate experiment, the temperatures were higher, in block division units of the heater 137, in the order of lanes 1 to 4, lanes 9 to 12, and lanes 5 to 8. When the top surface temperature of the sealing lid was measured for lane 1, which had the lowest temperature, and for lane 6, which had the highest temperature, the top surface temperature of the sealing lid of lane 1 was 13 C. lower than the set temperature, and the top surface temperature of the sealing lid of lane 6 was virtually the same as the set temperature. When the set temperature was 113 C., condensation was resolved at all lanes, and it can be inferred that the top surface temperature at lane 1 at this time was approximately 100 C.

(158) FIGS. 25A, 25B and 25C are drawings showing, when the material of the linking portions 131.sub.i is stainless steel, or when it is a resin with a lower thermal conductivity than stainless steel (PEEK), an improvement to the anti-fogging effect when they are linked with the PCR tubes 231.sub.i. FIG. 25A is a drawing showing a PCR tube 231 to which a linking portion 131 is linked. FIGS. 25B and 25C respectively show the temperature-fluorescence intensity characteristics when a stainless steel-made linking portion and a PEEK-made linking portion are used. The vertical axis represents the fluorescence intensity.

(159) In this experiment, in step S201, 20 L of a fluorescent reagent (0.25 L ROX) is dispensed into the PCR tubes 231.sub.i representing the reaction vessels, the apertures of which are sealed by the sealing lid 259, and the equivalent of twelve lanes are set to the temperature control block. Then, the linking portion 131 is mounted to the indentation section of the sealing lid. Here, the linking portions 131.sub.i have an outer diameter of 10 mm.

(160) In step S202, the block temperature is raised from 60 C. to 95 C. by means of the temperature control block, and the fluorescence intensity is measured for the respective lanes every 1 C. Consequently, in contrast to a marked decrease in the fluorescence intensity being observed at a PCR block temperature of approximately 80 C. or higher when a stainless steel-made linking portion is used, this is improved when a PEEK-made linking portion is used. Here, in comparison to the thermal conductivity (approximately 16.7 W/(m.Math.K)) of stainless steel (SUS303), the thermal conductivity of PEEK of approximately 0.25 W/(m.Math.K) is very low. Therefore, it is considered that the anti-fogging properties are improved as a result of a decrease in the heat release from the heater 137 that occurs by transmission to the linking portion, and the heating effect toward the sealing lid thereby improving.

(161) FIG. 26 is a drawing showing the thermal effects of heat transfer from the heater 137 imparted on an optical fiber 33 representing the light guide portion, which is provided within the linking portion 131 and surrounded by a ferrule 131c.

(162) In conclusion, the temperature within the ferrule is a maximum of 41 C. at the time of execution of temperature control by a thermal cycle. This is sufficiently lower than the heat resistance temperature of 70 C. of the optical fiber 33 representing the light guide portion, and it is considered that heat transfer from the heater imparts no effects on the degradation of the optical fiber.

(163) The experiment was performed using a thermo-recorder (KEYENCE NR-250(K05003)), an extra fine thermocouple 01-K manufactured by Ninomiya Electric Wire Co., Ltd. as a temperature sensor, and a ferrule (front end outer diameter: 3.5 mm, length: 32.5 mm) not containing a fiber.

(164) The thermocouple was inserted into the ferrule. A Teflon (registered trademark) tube was pressed into the ferrule, and one of the thermocouples was pressed and fixed against the inner wall of the ferrule. Two such ferrules were prepared and installed within the linking portion 131. The linking portions were mounted onto lane 6, which had the highest temperature on the heater 137, and lane 1, which had the lowest temperature. Then, after PCR temperature control to 95 C. (2 min), a thermal cycle that repeats 50 times a cycle from 60 C. (30 sec) to 95 C. (5 sec) was executed, and the temperature change within the ferrule was recorded. The temperature of the heater 137 was set to 113 C., at which condensation was eliminated.

(165) In FIG. 26, (1) represents room temperature, (2) represents the temperature within the apparatus, (3) represents the temperature of lane 1, and (4) represents the temperature of lane 6.

(166) As a result of this experiment, the temperature within the ferrule 131c at completion of the thermal cycle had risen by approximately 2 C. compared to the start. Therefore, the temperature within the ferrule at completion of the thermal cycle was a maximum of approximately 41 C. (steady state). Consequently, this temperature is sufficiently lower than the heat resistance temperature of 70 C. of the optical fibers 33.sub.i of the light guide portions, and it is considered that heat transfer from the heater 137 imparts no effects on the degradation of the optical fibers 33.sub.i within the linking portions 131.sub.i.

(167) FIG. 27 is a drawing showing extracted two adjacent specific wavelength measurement devices 140.sub.1 and 140.sub.2 among the six specific wavelength measurement devices 140.sub.j (j=1, . . . , 6) that correspond to the plurality of types of measurement devices 140 according to a fourth embodiment of the present invention.

(168) The specific wavelength measurement devices 140.sub.1 and 140.sub.2 are directly or indirectly linkable with the two or more reaction vessels 231.sub.i, and are provided corresponding to two or more linking portions 131.sub.i, to which are provided the front ends of two or more light guide portions 133.sub.i, which have a flexibility, that optically connect with the interior of the linked reaction vessels 231.sub.i. The configuration is such that the two or more connecting ends 134.sub.i, which are provided with the rear ends of the light guide portions that have the front ends provided to the linking portions 131.sub.i, are provided in the vicinity of, or making contact with, the arranging surface of the connecting end arranging body 130, which maintains them along the predetermined path in a specifiable array, and have one or two or more measuring ends 142.sub.1 and 143.sub.1 (142.sub.2 and 143.sub.2) that are successively optically connectable with the respective connecting ends 134.sub.i along the predetermined path. They are thus able to receive the light based on the optical state within the reaction vessels by means of an optical connection between the connecting ends 134.sub.j and the measuring ends 142.sub.i and 143.sub.i.

(169) There are provided corresponding to the respective specific wavelength measurement devices 140.sub.1 and 140.sub.2, at the very least between adjacent specific wavelength measurement devices, modulators 48.sub.1 and 48.sub.2 that modulate the intensity of the light of the specific wavelengths 1 and 2 to be received by the respective specific wavelength measurement devices at the mutually different predetermined frequencies A and B, and tuners 49.sub.1 and 49.sub.2 that perform tuning from the received light to the frequency of the light intensity of the modulated specific wavelengths 1 and 2.

(170) In a case where the light based on the optical state is a fluorescent light, the respective specific wavelength measurement devices 140.sub.1 and 140.sub.2 are provided with irradiation sources 146.sub.1 and 146.sub.2 that irradiate the predetermined excitation light 1 and 2 that excite fluorescent light of the corresponding specific wavelengths or specific wavelength bands 1 and 2, and light receiving portions 147.sub.1 and 147.sub.2 that are able to receive fluorescent light of the specific wavelengths or the specific wavelength bands 1 and 2. Generally, the wavelength of the excitation light is shorter than the specific wavelength .

(171) The irradiation sources 146.sub.1 and 146.sub.2 are provided with light emitting elements 1464.sub.1 and 1464.sub.2, such as an LED or an incandescent bulb, that emit light containing the corresponding excitation lights 1 and 2, and an excitation light 1 optical filter 1468.sub.1 and an excitation light 2 optical filter 1468.sub.2 that extract and transmit the excitation lights 1 and 2 from the emissions of the light emitting elements 1464.sub.1 and 1464.sub.2.

(172) The light receiving portions 147.sub.1 and 147.sub.2 are provided with a specific wavelength 1 optical filter 1477.sub.1 and a specific wavelength 2 optical filter 1477.sub.2 that extract and transmit light of the corresponding specific wavelengths or the specific wavelength bands 1 and 2, and light receiving elements 1474.sub.1 and 1474.sub.2, such as a photodiode, a phototransistor, a photomultiplier tube, a photoelectric cell, or a photoconductive cell, which convert the light transmitted by the specific wavelength 1 optical filter 1477.sub.1 and the specific wavelength 2 optical filter 1477.sub.2 into a corresponding electrical signal.

(173) Consequently, the modulators 48.sub.1 and 48.sub.2 have a frequency A oscillating voltage supply circuit 148.sub.1 and a frequency B oscillating voltage supply circuit 148.sub.2 representing the excitation light modulation portions, which supply to the irradiation sources 146.sub.1 and 146.sub.2 voltages that oscillate with a predetermined waveform at mutually different predetermined frequencies A and B, which are voltages for driving the light emitting elements of the irradiation sources 146.sub.1 and 146.sub.2. The modulators 48.sub.1 and 48.sub.2 also include the irradiation sources 146.sub.1 and 146.sub.2, and in addition, the reaction vessels 231.sub.i in which the fluorescent compound is housed.

(174) On the other hand, the tuners 49.sub.1 and 49.sub.2 tune the electrical signals converted from the light received by the light receiving portions 147.sub.1 and 147.sub.2 to the frequency A or the frequency B by passing them through a frequency A bandpass filter circuit or a frequency B bandpass filter circuit. Then, of the fluorescent light emitted based on the excitation lights 1 and 2 from the specific wavelength measurement devices 140.sub.1 and 140.sub.2, the light intensity data (electrical signals) for the frequency A or the frequency B that is received is extracted, which prevents crosstalk between adjacent specific wavelength measurement devices 140.sub.1 and 140.sub.2, and measurements with a high reliability can be performed.

(175) FIG. 28, which corresponds to FIG. 27, shows the movement direction between six pairs of measuring ends 142.sub.j and 143.sub.j of the plurality of types (six types in this example) of specific wavelength measurement devices 140.sub.j (j=1, . . . 6), and the connecting ends 134.sub.i of the connecting end arranging body 130.

(176) In FIGS. 29A, 29B, 29C, 29D, and 29E the respective modulators corresponding to the six pairs of specific wavelength measurement devices 140.sub.j (j=1, . . . 6) shown in FIG. 28 are set to the respective frequencies A to F (A, B, C, D, E, F)=(1 kHz, 4 kHz, 7 kHz, 7 kHz, 4 kHz, 1 kHz). In a case where the intensity of the excitation light is modulated as a blinking signal, a non-reflective sheet housed within the reaction vessels is irradiated instead of a sample, and data that is tuned to the respective frequencies from the electrical signal of the light received at a sampling rate of 5 ms is extracted. Then, the crosstalk between the specific wavelength measurement devices 140.sub.j was measured by measuring the intensity (data that corresponds to intensity data of the received fluorescent light) of the corresponding excitation light. Here, the intensity of the excitation light is overwhelmingly higher (on the order of several hundred times) than the intensity of the fluorescent light. Furthermore, the wavelength of the excitation light is short compared to the wavelength of the fluorescent light that is excited. However, if several types of fluorescent light are used, there are cases where the wavelength or the wavelength band of the excitation light overlaps with the wavelength or the wavelength band of the fluorescent light used in an adjacent measurement device that does not support the excitation light thereof. In such a case, there is a concern of the excitation light not being able to be excluded at the time the fluorescent light is received, generating crosstalk between the measurement devices.

(177) In the case of FIG. 29A (1), only the specific wavelength measurement devices 140.sub.1, 140.sub.2, and 140.sub.3 provided on the substrate 1 are set with mutually different frequencies A, B, and C (=1 kHz, 4 kHz, 7 kHz), and for the case where the respective excitation lights 1, 2, and 3 are irradiated, there is shown the intensities of the excitation lights received by the respective measurement devices as a result of leaks, scattering, reflection, and the like. In this case, as shown in the graph of (1), light of a constant intensity is respectively obtained. Therefore, crosstalk from the respective adjacent specific wavelength measurement devices was not observed.

(178) In the case of FIG. 29B (2), all of the specific wavelength measurement devices 140.sub.1, 140.sub.2, and 140.sub.3 provided on the substrate 1 and 140.sub.4, 140.sub.5, and 140.sub.6 provided on the substrate 2 are set with the frequencies A to F as described above. Then, the respective excitation lights 1, 2, and 3 are irradiated, and shown are the intensities of the excitation lights measured at the specific wavelength measurement devices 140.sub.1, 140.sub.2, and 140.sub.3 provided on the substrate 1. In this case, as shown in the graph of (2), the specific wavelength measurement device 140.sub.3 is such that crosstalk of the excitation light exists with the adjacent specific wavelength measurement device 140.sub.4 due to the excitation light being modulated at the same frequency of 7 kHz, and it is observed that there is a fluctuation in the intensity resulting from a combination of an offset in the phases of the modulated waveforms (blinking type) of the two devices.

(179) In the case of FIG. 29C (3), shown is a case where all of the specific wavelength measurement devices 140.sub.1, 140.sub.2, and 140.sub.3 provided on the substrate 1, and only the specific wavelength measurement device 140.sub.6 provided on the substrate 2 are driven, and a measurement is performed at the specific wavelength measurement devices provided on the substrate 1. As shown in the graph of (3), crosstalk of the excitation light is not observed since the spacing between measurement devices having the same frequency is long.

(180) In the case of FIG. 29D (4), shown is a case where all of the specific wavelength measurement devices 140.sub.1, 140.sub.2, and 140.sub.3 provided on the substrate 1, and only the specific wavelength measurement device 140.sub.5 provided on the substrate 2 are driven, and a measurement is performed at the specific wavelength measurement devices provided on the substrate 1. As shown in the graph of (4), crosstalk of the excitation light is not observed.

(181) In the case of FIG. 29E (5), shown is a case where all of the specific wavelength measurement devices 140.sub.1, 140.sub.2, and 140.sub.3 provided on the substrate 1, and only the specific wavelength measurement device 140.sub.4 provided on the substrate 2 are driven, and a measurement is performed at the specific wavelength measurement devices provided on the substrate 1. As shown in the graph of (5), the specific wavelength measurement device 140.sub.3 is such that crosstalk of the excitation light exists with the adjacent specific wavelength measurement device 140.sub.4 due to the excitation light being modulated at the same frequency of 7 kHz, and it is observed that there is a fluctuation in the intensity resulting from a combination of an offset in the phases of the modulated waveforms of the two devices.

(182) In FIG. 30, shown is a case where, in accordance with 96-well microplates that are utilized as an international standard, the linking portions 131 are arranged with a 9 mm pitch corresponding to the pitch of 9 mm when the PCR tubes 231, representing the reaction vessels are arranged, and the spacing of the connecting ends 134.sub.i on the arranging surface of the connecting end arranging body 130 is integrated to 3.75 mm when the diameter of the optical fiber 133, which represents the light guide portion, that is arranged on the connecting end arranging body 130 with one end thereof provided to the linking portion 131, and the other end to the connecting end 134, is 1.5 mm. Even with such a spacing, as shown in FIG. 31, the data of the light received by the measuring end 143.sub.1 of a given specific wavelength measurement device 140.sub.1 of the measurement device 140 identifies with certainty the light from the eight connecting ends.

(183) FIG. 32 is a drawing showing a connecting end arranging body 1300 according to a fourth embodiment of the present invention, and the movement thereof The respective linking portions 131.sub.2 to 131.sub.12 are provided with the front ends of light guide portion bundles 133.sub.2 to 133.sub.12 comprising a plurality of light guide portions. Among the light guide portion bundles, a portion of the rear ends of the light guide portion bundles is provided to a first connecting end of the connecting end arranging body 1300, and the remaining light guide portion bundles are provided to a second connecting end of the connecting end arranging body 1300. The predetermined path comprises a linear first path and a linear second linear path for example. The connecting end arranging body is such that, with regard to the eleven pairs of first connecting ends and second connecting ends that are optically connected with the eleven linking portions 131.sub.2 to 131.sub.12, and a single pair of third connecting ends and fourth connecting ends that are optically mutually connected by means of a light guide portion 133.sub.0, the eleven first connecting ends and the single third connecting end are arranged along the linear first path, and the eleven second connecting ends and the single fourth connecting end are arranged along the linear second path, which is parallel to the first path leaving a predetermined spacing. In this example, for simplicity, the connecting end arranging body 1300 of the present embodiment has been achieved by removing the light guide bundle 133.sub.1 provided to the first pair of the first connecting end and the second connecting end of the connecting end arranging body 130 according to the embodiment described above, and further replacing it with the light guide portion 133.sub.0 that optically mutually connects between the third connecting end and the fourth connecting end.

(184) As a result of movement of the connecting end arranging body 1300, the first measuring ends 140.sub.1 respectively provided to the measurement devices 140.sub.1 to 140.sub.6 relatively move along the first path comprising the eleven first connecting ends and the single third connecting end, and the second measuring ends respectively provided to the measurement devices 140.sub.1 to 140.sub.6 relatively move along the second path comprising the eleven second connecting ends and the single fourth connecting ends, respectively. The first measuring ends are optically connected with the irradiation source of the measurement device, and the second measuring ends are connected with the light receiving portion of the measurement device. In contrast to a device in which the pairs of first measuring ends and second measuring ends respectively provided to the measurement devices 140.sub.1 to 140.sub.6 are integrally provided, as a result of movement of the connecting end arranging body 1300, for each pair, the first measuring ends of the respective measurement devices successively connect with the eleven first connecting ends and the single third connecting end, and at the same time, the second measuring ends of the respective measurement devices successively connect with the corresponding eleven second connecting ends and the single fourth connecting end. Therefore, together with measurements of the fluorescence intensity by the eleven pairs of first connecting ends and second connecting ends, it becomes possible, by means of the single pair of the third connecting end and the fourth connecting end, for the light receiving portion to directly receive the excitation light via the light guide portion 133.sub.0 without it being diverted to the reaction vessels, and a genuine excitation light intensity can be measured.

(185) FIG. 33A is a graph plotting, in a case where the temperature of the surroundings of the LED has been intentionally raised (by stopping the cooling fan for example), the intensity of excitation light measured by the light receiving portion using the pair of the third connecting end and the fourth connecting end, and data measured for the intensity of fluorescent light obtained from the respective reaction vessels by irradiating excitation light using the eleven pairs of first connecting ends and second connecting ends with respect to the reaction vessels via the linking portions 131.sub.2 to 131.sub.12. In the case of this example, the center wavelength of the LED is 590 nm, and a modulated frequency of 7 kHz is added to the excitation light of the LED circuit. Furthermore, distilled water is housed in the respective tubes, and after heating for 5 min at 95 C., a temperature control of 95 C. for 5 sec and 60 C. for 30 sec is repeated for 80 cycles. FIG. 33B is a graph plotting the fluorescence intensities, which have been normalized by removing a fluctuating component of the excitation light by dividing the fluorescence intensity by the measured intensity of the excitation light.

(186) As shown in FIG. 33A, together with the temperature increase of the LED, the intensity of the excitation light measured by the receiving portion using the single pair of the third connecting end and the fourth connecting end is gradually becoming weaker. Consequently, in that case, the nature of the fluctuations in the intensity of the excitation light and the respective fluorescent light from 5 min to 80 min after stabilization of the intensity is expressed as approximately 1.89% to 1.37% as a CV value, in which the standard deviation is divided by the average value. In contrast, as shown in FIG. 33B, looking at the value of the intensity of the fluorescent light divided by the intensity of the excitation light measured using the single pair of the third connecting end and the fourth connecting end, the nature of the fluctuations in the intensity of the fluorescent light shows approximately 0.40% to 0.63% as a CV value. Therefore, the fluctuations in the fluorescence intensity are small. That is to say, it shows that fluorescence intensities that have excitation light fluctuations removed, which are mainly determined by the fluorescent material concentration, are obtained.

(187) FIG. 34 shows specific wavelength measurement devices 1400.sub.1 and 1400.sub.2 in a case where, as tuners 49.sub.1 and 49.sub.2, lock-in amplifiers 1490.sub.1 and 1490.sub.2 are used instead of bandpass filter circuits. In this case, the reference signal can utilize the outputs of the frequency A and B oscillating voltage supply circuits 148.sub.1 and 148.sub.2 of the modulators 48.sub.1 and 48.sub.2. Therefore, the configuration is simplified as a whole, and measurements with a high reliability can be performed.

(188) The foregoing exemplary embodiments have been specifically described in order to better understand the present invention, and they are in no way limiting of other embodiments. Therefore, modifications are possible within a scope that does not depart from the gist of the invention. The configurations, shapes, materials, arrangements, numbers, and amounts of the nozzles, the dispensing tips, the punching tips, the vessel group, the exclusive regions thereof, the housing parts, the measuring ends, the measurement devices, the specific wavelength measurement devices, the suction-discharge mechanism, the transfer mechanism portion, the magnetic force part, the heating portion, the reaction vessels, the sealing lids, the light guide stage, the linking portions, the light guide portions, the connecting ends, the connecting end arranging body, the linking portion arranging body, the nozzle head, the temperature controller, the nozzle detaching mechanism, the sealing lid detaching mechanism, the modulators, the tuners, and the like, and the utilized reagents and samples are also in no way limited by the examples illustrated in the exemplary embodiments. Furthermore, although the nozzles were made to move with respect to the vessel group, it is possible to also move the vessel group with respect to the nozzles.

(189) Furthermore, in the foregoing descriptions, although the amplification solution was sealed using a sealing lid for the sealing of the reaction vessel for PCR, it may be made such that, in its place or in combination, it is sealed using a sealing liquid, such as mineral oil. Furthermore, in place of punching by mounting a tip for punching on the nozzles, it is possible to use a punching pin that is driven by the suction-discharge mechanism. Moreover, although a real-time PCR measurement was described in the foregoing descriptions, it is in no way limited to this measurement, and it can be applied to other various measurements in which temperature control is performed. In the foregoing descriptions, although a case where the measurement device is provided to a dispensing device was described, it is not necessarily limited to this. Although only an optical system using optical fibers was described, it is possible to also employ an optical system using a lens system in the interior of the measurement device.

(190) Furthermore, the apparatuses described in the respective exemplary embodiments of the present invention, the components that form these apparatuses, or the components that form these components, can be appropriately selected, and can be mutually combined by applying appropriate modifications. The spatial representations within the present application, such as above, below, interior, exterior, X axis, Y axis, and Z axis are for illustration only, and are in no way limiting of the specific spatial directions or arrangements of the construction.

INDUSTRIAL APPLICABILITY

(191) The present invention is related to fields in which the processing, testing, and analysis of nucleic acids, which primarily includes DNA, RNA, mRNA, rRNA, and tRNA for example, is required, and is related to industrial fields, agricultural fields such as food, agricultural products, and fishery processing, chemical fields, pharmaceutical fields, health care fields such as hygiene, insurance, diseases, and genetics, and scientific fields such as biochemistry or biology for example. The present invention is, in particular, able to be used in processing and analysis that handles various nucleic acids, and the like, such as PCR and real-time PCR.

BRIEF DESCRIPTION OF THE REFERENCE SYMBOLS

(192) 10, 110 Light measurement apparatus for reaction vessel 20, 120 Vessel group 20.sub.i, 120.sub.i (i=1, . . . , 12) Exclusive regions 211.sub.i (i=1, . . . , 12) Dispensing tips 231.sub.i, 236.sub.i (i=1, . . . , 12) PCR tubes (reaction vessels) 30, 300, 130, 1300 Connecting end arranging body 31.sub.i, 131.sub.i (i=1, . . . , 12) Linking portions 32, 320, 132 Light guide stage 33.sub.i Optical fibers (example of light guide portions) 40, 400, 140 Measurement device 40.sub.j, 400.sub.j, 1400.sub.j (j=1, . . . , 6) Specific wavelength measurement devices 44 Measuring end 48.sub.j Modulator 49.sub.j Tuner 50, 500, 150 Nozzle head 52 Transfer mechanism portion 53 Suction-discharge mechanism 59 Tip detaching mechanism 61, 161 Measurement control portion 70 Nozzle arranging portion 71.sub.i (i=1, . . . , 12) Nozzle